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1 Introduction
A fuel cell that uses microorganisms as catalysts for the oxidation of substrates, leading to electricity generation, is termed a microbial fuel cell (MFC). They are deployed for sustainable energy production, treatment of wastes, and minimizing CO2 emissions. There is a compelling need for energy- and cost-efficient alternates to the conventional wastewater treatment systems due to their high energy requirements. Domestic and industrial effluents contain a multitude of organic compounds that can fuel MFCs, and the microbes in MFCs can accomplish both pollutant degradation and power generation in parallel. Moreover, it minimizes solid disposal by 50–90% [23] and hence is generally accepted as a promising sustainable biotechnological solution to future energy requirements. However, it is yet to be commercially exploited since there are many hurdles to be overcome. This chapter mainly focuses on the application of MFCs toward the removal of environmental pollutants, such as synthetic dyes and polycyclic aromatic compounds. Recent progress in the understanding of system architecture, anode/cathode materials, and biocatalysts employed in MFCs for pollutant degradation to achieve high energy density has been reviewed.
2 Bioremediation of Petrochemicals Through Microbial Fuel Cells
The ever-growing global economy and population have ensured an increasing demand for petroleum products, thus aiding the growth and multiplication of relevant industries worldwide [101]. The environmental impact of petroleum products is a correspondingly vast and extensive field. Petroleum hydrocarbons are harmful to many soil organisms and humans. This fossilized fuel has become a necessary source of energy since the late nineteenth century due to elevated consumption globally. The petrochemical industry involves the production of solvents, resins, lubricants, and plastics, generating wastewater containing toxic compounds [79]. In the downstream sector, wastewater from petroleum industries and refineries contains phenolic compounds, aromatic hydrocarbons, salts, alkanes, naphthalene, nitrobenzene, oil, and grease. These pollutants are toxic, not biodegradable, and carcinogenic [17]. In the upstream sector, the exploration and production process of conventional crude oil generates the largest volume of waste stream termed Produced water (PW). Typically, PW contains emulsified oils besides a high amount of sodium chloride, carbonates of magnesium, sulfates, and other inorganic dissolved and suspended solids. Benzene, xylenes, ethylbenzene, and toluene are hazardous chemicals, also known as BTEX materials. They are a threat to human health and are common PW water contaminants [121]. Petroleum product contamination degrades the ecological structure and function of the soil, affecting moisture, pH, carbon/nitrogen ratio, and porosity. As the pollutant concentration rises, the hydrophobicity increases, thereby inhibiting the seed germination [39]. The aromatic hydrocarbon groups of compound derivatives have molecular rings, including benzene rings. Usually, instead of a hydrogen atom in the benzene ring, methyl or ethyl groups are found (toluene, methyl/dimethyl benzene and ethylbenzene) [92]. These hydrocarbons, referred to as polycyclic aromatic hydrocarbons (PAHs), can cause musculoskeletal malformation and bone marrow suppression [93]. PAHs toxicology and physicochemical characteristics are varied according to their molecular weight [104]. Recent studies demonstrate that benzenes can easily bind to soil particles, and BTEX chemical compounds need sufficient oxygen to break down slowly. These compounds are volatile and hence can affect the human respiratory system [58]. Hence, researchers have taken great efforts to upgrade the different technologies used to treat wastewater from the petroleum industry, such as adsorption, coagulation, membrane separation process, dissolved air flotation, and chemical destabilization [103]. However, these unit operations are ineffective for the treatment of petroleum wastewater because of the complexity of PAHs. Although incineration and chemical oxidation can remove up to 99.0 and 92.3% of the total petroleum hydrocarbons from wastewater, respectively, both these restoration procedures have disadvantages. Toxic substances, such as dioxins, furans, polychlorinated biphenyls, and volatile heavy metals, are released into the atmosphere as a result of the incomplete burning of crude oils [19]. Therefore, an effective remediation measures should be tailored considering the human health and ecosystem management.
The biological treatment of petroleum wastewater can be accomplished by aerobic and anaerobic processes. However, the anaerobic process is preferred over aerobic due to lower energy consumption and less sludge production, though it is time-consuming [1, 102]. Bioelectrochemical systems (BES) are versatile [89] because, in contrast to typical bioreactors, they can concurrently generate electricity and treat wastewater. Similarly, in recent years, an emerging technology based on biostimulation, namely microbial fuel cells (MFCs), has been demonstrated to remove PAHs from soils and sediments.
2.1 MFCs for Petroleum Product Degradation
2.1.1 In-Situ Bioremediation of PAH-Contaminated Soil in Microbial Fuel Cells
In situ bioremediation is a biological treatment method to clean up PAHs present in the environment. The potential advantages of in-situ bioremediation are (i) minimal site disruption, (ii) continuous treatment of contaminated soil and groundwater, and (iii) economical. The anaerobic degradation of petroleum compounds has been identified in a wide spectrum of microbial pathways, including denitrification [4, 51], metal reduction [52], and sulfate reduction [84]. MFC configurations with different electrode materials for in-situ bioremediation to remove hydrocarbons from soils and sediments as shown in Table 1. Yuan et al. [113] developed a tubular air-cathode MFC (TAC-MFC) with a cloth cathode assembly and utilized organic sediment as the substrate, demonstrating the highest power density of 107.1 ± 8.6 mW/m2, coulombic efficiency of 17.9%, and the lowest internal resistance (20 Ω). Bioremediation of PAHs in sediments through MFC technology is shown in Fig. 1; the anodic reactions include the oxidation of organic matter, sulfides, and other potential pollutants. The color of the sediment apparently turned from black to brown after MFC operation in the contaminated sites. Huang et al. [25] reported enhanced phenol biodegradation and simultaneous electricity production from organic pollutants in a soil MFC (Fig. 2). An in-situ MFC was inserted into waterlogged anoxic soil for the remediation of organic pollutants, and the power reached 29.45 mW/m2 with 90.1% removal of phenol. The phenol degradation rate in this BES was approximately 23 times higher than biological treatment. Similarly, another bioelectrochemical treatment (BET) system showed 41.08% petroleum hydrocarbon removal compared to 20.72% obtained using conventional anaerobic treatment with petroleum sludge as the substrate [59]. Wang et al. [105] demonstrated a U-tube MFC for the bioremediation of PAHs-contaminated soil. Due to the presence of exoelectrogenic biofilms on the surface of the anode, it could effectively remove n-alkanes and PAHs from the soil. However, the power density was low (0.85 + 0.05 mW/m2), suggesting that the U-tube air-cathode MFC was not as suitable for soil remediation systems.
To increase the degradation efficiency of total petroleum hydrocarbons (TPH) [117], varied the arrangement of anodes from vertical to a horizontal position in a soil MFC (Fig. 3 and found that the horizontally arranged anodes (50.6% showed superior performance than the vertical counterparts (8.3%. Charge output was also higher in the horizontal anode configuration (833 C compared with the vertical mode (762 C. The PAH degradation rate increased from 2 to 24% in 66 d; however, low power output was observed in the in-situ MFCs with vertical anodes (an average of 37 mWm−3). In most cases, the internal resistance of the in-situ MFC was high was mainly due to the high ohmic resistance i.e., low soil conductivity and mass transfer limitations [67]. To overcome these issues, the amendment of PAH-contaminated soil with different additives, such as sand, carbon fibers, and biochar, was adapted to enhance the bioelectrochemical remediation of PAHs. Amendment with sand increased the soil porosity from 44.5 to 51.3%, decreased Ohmic resistance by 46%, and increased the charge output from 2.5 to 3.5 C g−1 [42]. The porosity of the soil plays a significant role in increasing both dissolved oxygen content and proton transport, thereby decreasing charge transfer resistance. Moreover, the internal resistance of the soil MFC decreased by 58% after mixing 1% carbon fiber with the soil samples, leading to a 15-fold increment in electricity production and 329% higher removal of petroleum hydrocarbons [44, 45]. Similarly, the addition of biochar with high N content accelerated the selective enhancement of the bacterial community and exhibited the best efficiency in the removal of recalcitrant contaminants, such as aromatics [47]. A plant microbial fuel cell (PMFC) is a promising modified MFC that fosters plant-microbe relationship at the rhizosphere region of a plant, which consequently increases the biofilm adhesion on the anode, thereby improving electron transfer from the biofilm to the anodes in the PMFC. The synergistic action of plants and surfactants significantly enhances the efficiency of the MFC system in the removal of petroleum from the soil and encourages the applications of P-MFCs for the in-situ remediation of petroleum-contaminated soils. Zhao et al. [118] used plant MFC to degrade phenanthrene and pyrene in contaminated soils. The addition of surfactant β-cyclodextrin enabled the desorption of PAHs from the soil to the plant at a higher rate than Tween 80 and decreased the charge transfer resistance by 63.4%. This suggests that the amendment of contaminated soil with some additives, which possess the characteristics of conductivity and micronutrient, enhances the removal of petroleum from the soil in MFC systems.
2.1.2 Bioremediation of Petroleum Wastewater in MFCs
Petroleum hydrocarbons are very common groundwater contaminants that usually deplete oxygen when they undergo biodegradation. The anaerobic degradation of petroleum compounds has been identified to involve a wide spectrum of microbial pathways, including denitrification [4, 51], metal reduction [52], and sulfate reduction [84]. The bioremediation of petroleum waste, including wastewater from petroleum refineries, petroleum sludge, and oil-contaminated soil, has been studied in MFCs (Table 2). The downstream petroleum refinement processes generate large volumes of wastewater containing hydrocarbons, such as phenols, benzene, and xylenes, as well as inorganic compounds, such as ammonia, nitrite, sulfides, and heavy metals. Wastewater from petroleum refineries can range from 0.4 to 1.6 times the volume produced during crude oil processing [9] The low BOD/COD ratio (<0.2) and high total dissolved solids (TDS) render the petroleum refinery wastewater (PRW) unsuitable for biological treatment.
In 2008, Morris and Jin reported, for the first time, MFC technology for the degradation of hydrocarbon contaminants in groundwater under anaerobic conditions. Using refinery wastewater as the sole substrate, they achieved power generation (as high as 120 mW/m2 at the cathode) in approximately ∼6 d with a single cell MFC. It is known that the larger the electrode spacing, the potential losses in the cell are higher. The authors reported that the cell potential decreased by ∼55% over a 9 m-long proton bridge, with a 6.9% decrease in potential per meter of the bridge [66]. To enhance the treatment of PRW in a single-chamber air-cathode microbial fuel cell (MFC), external voltage from 100 to 500 mV was applied to the electrochemically active anodic biofilm [60,53,62]. The MFC operated with 500 mV supplemental voltage exhibited a maximum power density of 132 mW/m2, which was three times higher than control MFC (45 mW/m2). Similarly, the highest substrate removal efficiency (48%) was obtained with the MFC supplemented by 500 mV, followed by those supplied with 300 mV (37%), 100 mV (32%), and the 0 V control (27%). Therefore, the applied potential enhanced the diesel-range organics (DROs)/straight chain-alkane degradation efficiency. [88] proposed the coupling of a hydraulically connected osmotic microbial fuel cell (OsMFC) and an up-flow microbial desalination cell (UMDC) for enhanced PAH removal, along with high power output in the MFC and simultaneous seawater desalination in the UMDC (Fig. 4). Both OsMFC and UMDC were connected to 100 Ω external resistance, and the system achieved 93% chemical oxygen demand (COD) removal from the petroleum refinery wastewater besides 48% salt removal from the seawater. Sarmin et al. [87] treated the petroleum chemical wastewater from an acrylic acid plant using an MFC, which generated 850 mW/m2 maximum power density at 1500 mA/m2 current density and 45,000 mg L−1 COD. Produced water (PW) refers to the large quantity of wastewater generated during oil and gas extraction. PW is usually characterized by high amounts of dissolved solids (TDS) and residual petroleum hydrocarbons, which are considerably hazardous to the environment. The level of environmental damage is dependent on the geographical location, age of the well, nature of geological formations at the specific location, the type of hydrocarbon products found the well and the stage of oil extraction. The bioelectrochemical treatment of PW was studied by Mohanakrishna et al. [64, 65] in two configurations viz., single- and dual-chamber microbial fuel cells (MFCs), of which the latter configuration displayed superior function with respect to power generation (1089 mW/m2) and substrate degradation (COD removal efficiency = 60.2%) compared with the single-chamber configuration (PD 789 mW/m2; COD removal efficiency = 54.7%); However, since the nature of PW varies based on the location and oil extraction stage, intensive studies are required to develop suitable processes with reference to the operating conditions; further, understanding the microbiology in connection with the composition of PW is also essential.
2.1.3 Microorganisms for PAH Treatment in Fuel Cells
A summary of the different microbial species employed in MFC operation for specific PAH substrates and their possible performance parameters are given in Table 3.
The hydrocarbon-degrading bacterial strain Alcanivorax has been shown to simultaneously improve bioelectricity generation and bioremediation performance in soil MFCs [42]. Li et al. [44, 45] suggested that employing the Alcanivorax strain is an effective approach to eliminate soil contamination in barren areas/extreme environments. Hassan et al. [22] found that various exoelectrogens, including Arcobacter, Aeromonas, Pseudomonas, Acinetobacter, Cloacibacterium, and Shewanella sp., were involved in the degradation of 2, 4-DCP (dichlorophenol). This study discovered that Cloacibacterium sp. can degrade phenol in MFCs. Sarmin et al. [87] utilized pre-acclimatized mixed culture inoculum composed of electrogenic genera, such as Bacillus sp., Pseudomonas sp., and Methanobacterium sp. (methanogenic archaea), as biocatalysts in treating petrochemical wastewater (PCW) from an acrylic acid plant. This suggests that MFCs could be potential alternatives to conventional aerobic and anerobic processes, which are high energy-intensive.
2.1.4 Influence of Co-substrates and pH in MFCs for PAHs Degradation
The cultivation medium used for microbial consortia in MFC systems generally contains co-substrates to drive microbial growth during operation. Most studies have reported the use of co-substrates along with petroleum refinery wastewater in the anode chamber [22, 24, 44, 45, 66, 114, 116, 118]. In addition [60,53,62], used acetate as a co-substrate and it can be inferred that the substrate can stabilize microbial activity in an anode and continue electricity output at a steady current density level. pH is a crucial factor for anaerobic processes and serves as an intrinsic index of the changes in biological systems. Srikanth et al. explained that in batch mode operation, a change in pH toward the acidic range (pH < 4.0) [94] can be attributed to the competing action of mixed consortia in the redox reactions. Mohanakrishna et al. [60,53,62] reported that a single-chamber air-cathode MFC operated with PRW as the anolyte showed negligible change in the pH of the effluent. However [113], explained that the pH of all sediments slightly decreased, probably due to the fermentation of organics. Mohan and Chandrasekhar [59] Mohan & Chandrasekhar et al., demonstrated that the system pH was strongly controlled by the microbial metabolic rate, bioavailability, and mass transfer. In soil MFCs, the depletion of existing organic acids and the formation of bicarbonate salts leads to an increase in soil pH [26]. Li et al. [42] showed that pH values of the soil effectively correlated with the addition of sand as vast pH shifts were observed with increasing sand quantities. Li et al. [44, 45] elucidated that the addition of glucose substrate can neutralize soil pH and improve conductivity, and then the glucose is partly metabolized to organic acids (such as acetate). Soil pH and conductivity were the prominent factors that controlled the polyphenol oxidase and dehydrogenase activities, thus increasing TPH degradation and power output.
3 MFC for Dyes Degradation
Dyes containing one or more azo bonds are widely used in textile, leather, plastics, cosmetics, and food industries [74]. The color of these dyes is due to the azo bond and associated chromophores; so, the disposal of these dyes into the surface water not only affects the aesthetics, but their breakdown products have often been found to be carcinogenic, mutagenic, or toxic for humans [18]. These dyes can also obstruct the light and oxygen penetration into water, thus affecting the aquatic life. Several methods for the treatment of dye-containing wastewater which broadly fall into three categories: physical (adsorption, coagulation/flocculation, membrane filtration etc.), chemical (chemical oxidation, photo-catalytic oxidation, electrolysis, Fenton reagent etc.), and biological (biosorption, enzymatic degradation etc.) [34]. These physicochemical methods can be quite effective for color removal, but they have inherent disadvantages, including high operational costs and the generation of huge quantities of sludge for disposal. Recently, attempts have been made to address this issue through the biological route, using specific microorganisms for the cleaving of the azo bond. The biological treatment for the decolorization and degradation of azo dyes may be either aerobic, anaerobic, or a combination of both, depending on the type of microorganisms being employed [5, 35]. Most of the azo dyes are decolorized under anaerobic conditions however the anaerobic reduction is generally very slow, and produce methane rich gas. In most cases, the aromatic amines derived from azo dyes are not degraded under anaerobic conditions due to its high redox potential. These product amines released from azo dye decomposition are known, or suspected, to be more carcinogenic for humans [81] than the parent compound azo dyes. Thus, MFC has been employed for performing the dual duty of degrading the textile dyes and generating power. Moreover, such a system can reduce solids generation by 50–90% and reduce the load on their disposal. Based on their structure, dyes are classified into: acid/basic dyes, direct dyes, azo dyes, sulfur dyes, fiber reactive mordant dyes, etc., [38]. Dye molecule consists of two parts: (1) chromophores, which are functional groups of dyes, and (2) auxochromes, which enhance the color. Azo dyes account for more than 70% in the textile effluent. Most of the studies have focused the dye decolorization in the anode of MFC and few reports on dye decolorization in the cathode [27].
3.1 Azo Dye Decolorization and Degradation in the Anode
The co metabolism reaction is probably the main mechanism of dye degradation at the anode in which the reducing equivalents (electrons) are formed during the anaerobic oxidation of co-substrate. Once the substrate was oxidized and transferring the some portion of electrons for the exoelectrogens accumulated on the anode that pass through an external circuit producing current. Simultaneously, the other part of the electrons transferred to split the −N=N− bond in the azo dyes to form aromatic amines in the anode chamber. Hence, there will be a competition for electrons between dye substances and an anode in an MFC. Different dyes were tested as anolyte with different substrates in MFC as shown in Table 4.
Electricity generation from glucose as co-substrate accompanied by decolorization of azo dye active brilliant red X-3B (ABRX3)was investigated by Sun et al. [97] using a microfiltration membrane air-cathode single-chamber microbial fuel cell (MFC). Around 90% of the dye was removed within 12 h at an dye concentration of 100 mg/l. The dye degradation mechanism via MFC technology as shown in Fig. 5. Anaerobic-aerobic sequential MFC reactor couple system was developed for Congo red degradation [48].
It was observed that the amines are formed in the anode chamber, and they are resistant to further degradation in the MFC under an anaerobic environment. To overcome this, a novel aerobic biocathode was designed by Sun et al. [95], since these intermediates are transferred to the cathodic chamber and further biodegradation takes place under aerobic conditions. Degraded intermediates transferred to the biocathode resulted in an almost 150% increase in open cycle potential (OCP) of the cathode accompanied by a 73% increase in stable voltage output from 0.33 to 0.57 V and a 300% increase in maximum power density from 50.74 to 213.93 mW/m2. Azo dyes removal in CW has been investigated by Noonpui and Thiravetyan [73] and they demonstrated that a maximum 97% of dye removal in the constructed wetland (MFCCW); however, the removal efficiency was found to be a function of structure and size of the dye molecule. Moreover, they emphasized that the plant, soil, and microorganisms might all influence the efficiency of dye removal in a CW.
Double chambered MFCs are the most common design in MFCs studies which separated by a PEM. The protons transfer through PEM can be a limiting factor due to the suspended solids and soluble contaminants [28]. Moreover, double chambered MFC is difficult to scale-up. Single chambered up-flow membrane-less microbial fuel cell (UFML MFC) was developed by Thung et al. [100] for decolorization of Acid Orange 7 (AO7) and electricity generation simultaneously. Azo bond and naphthalene moieties were completely degraded but aromatic amines were remained in the effluent and however the COD and color removal by the bioreactor was up to 90%. The azo dyes (methyl orange (MO), Congo red (CR), reactive yellow (RY), reactive red (RR), along with nutrient medium were tested as anolytes in the Pseudomonas-catalyzed MFC at an external resistance of 220 Ω [29]. In the anode chamber maintained under anaerobic conditions more than 90% of MO and RB could be decolorized by P. aeruginosa in 5 d, but RR was decolorized only about 74%. Generally, the microorganisms produce azoreductases which reductively cleave the highly electrophilic azo bond at the expense of a reducing agent, typically NAD(P)H [54]. It is expected that reduction under anaerobic environments will be nonspecific, although the rate of decolorization depends on the organic source and the dye structure (Engineering 2012). It suggested that a simple structure and low-molecular weight azo dye MO degraded faster in MFC than CR, RR, and RY which are highly substituted and of high molecular weight. On the other hand, it was also observed that bacterial decolorization of di azo RB was more superficial when compared with either of the mono azo dyes (RR, RY). The presence of two electron withdrawing group (SO3 −1) at ortho position to azo bonds and OH group of the naphthol ring in RB were also the main aspect of the azo bond cleavage. The sulfo groups could withdraw electrons from azo bond by resonance, and the environment in the proximity of azo bond could become more electrophilic for decolorization [115].
Reduction of azo dye in the anode chamber might be performed either by direct enzymatic reaction or mediated reaction of enzyme cofactors, which are biologically regenerated by sulfate-reduction bacteria (SRB) to break down the azo bond [85]. Santos et al. [11] used single-chamber air-cathode microbial fuel cells to investigate the interaction mechanisms among Congo red decolorization, sulfide oxidation, and bioelectricity generation. The results showed that effective removal of sulfide (98%) and azo dyes (88%) was achieved at an initial sulfide/dye ratio of 0.9 under neutral conditions, accompanied by a maximum power output of approximately 23.50 mWm−2.
3.2 Azo Dye Decolorization and Degradation in the Cathode
The color of dyes is due to azo bond and associated chromophores, so disposal of dyes into surface water not only affects the aesthetic but cause also biotoxicity. The azo dye was used in the cathode with the dual objective of solving as facilitator for electron acceptor and the possibility of decolorization. The cathode chamber was continuously sparged with N2 gas to avoid O2 competition with the azo dyes for electrons. The reduction reactions in the cathode chamber are described by Goyal and Minocha [20], Menek, Zeyrekl, and Karaman n.d.; Parthasarathy and Narayanan [78] in which the –N=N– double bond was reduced to hydrazo (1) or amine (2), via the consumption of two or four electrons.
Mu et al. [68] achieved an effective decolorization rate of dye was 2.64 ± 0.03 mol m−3 d−1 achieved at the cathode of a bioelectrochemical system while acetate was used in the anode [68]. Reduction of azo dyes in the cathode of MFC using electrons produced from metabolic oxidation of Klebsiella pneumoniae strain L17 in the anode [50]. Different azo dyes congo red, methyl orange and reactive blue, were tested as catholyte in Pseudomonas-catalyzed MFC using a graphite block [29]. Compared to potassium ferricyanide (72.8 ± 17.8 µW/m2) and potassium permanganate (86.28 ± 16.8), addition of azo dyes enhanced the power output by several fold (congo red-91.73 ± 21.2; Methyl orange 314.07 ± 22.6; Reactive blue 172 294.43 ± 11.9 µW/m2). Lot of research findings (Table 5), the degradation efficiency was high; however it was found that low power density resulting from the small specific capacitance of the carbon-based materials.
3.3 Key Parameters Influencing the MFC for Dyes Degradation
Developing an MFC for dye waste water treatment with a simple configuration at affordable costs for the ease of scale-up still remains the challenge. Many workers have reported that the several factors that affect the MFC performance including the microbes, the proton selective membrane, the material of the electrodes, the cathode/anode catalyst, the electrode spacing, and the system design [31]. It is known that electron transfer between the electron carriers in the bacteria transport chain and the anode/cathode is low, leading to a high internal resistance that reduces the power generated in MFCs.
3.3.1 Effect of Co-substrate
One of the most crucial aspects of MFC is the substrates used due to their effect on the dye degradation and power output. A report by [97], when confectionary and glucose wastewater were utilized as co-substrates in MFC along with a model azo dye, there is a competition between the anode and azo dye reduction for electrons from co-substrate oxidation. Bioelectricity generation with a simultaneous Congo red degradation can be achieved using glucose, acetate sodium, and ethanol as co-substrate in PEM air-cathode single-chamber MFC. More than 98% of the Congo red (300 mg/L) was decolorized within 36 h for all tested co-substrates during the electricity generation. Glucose produced the highest power densities when compared to Ethanol [6, 57] studied that simultaneous electricity generation and tetra-azo dye (Direct Red 80) decolorization was examined in a dual-chamber MFC. Glucose was identified as a better co-substrate than acetic, propionic, and lactic acid for dye removal and current production (477.8 mW/m2). It can be understood that the low-molecular co-substrates can be easily degraded, excessive co-substrate addition can lead to high COD, in addition to resource (dye) waste. Anaerobic azo dyes degradation in MFC are strongly influenced by co-substrate type and its concentration suggested that the combination of dye containing waste water and high organic content waste water from food/biorefinery industries that is easily biodegraded simultaneously that can both improve cost and energy.
3.3.2 Effect of PH
Anodic pH microenvironment is one of the important factors which can influence substrate metabolic activity and as well as proton transfer mechanism [80]. pH has a significant factor on dye degradation efficiency because of enzymatic activity depends on the pH. The color of the solution and solubility of the dyes is affected by the pH. The release of metabolic products by a biocatalyst into the anolyte will lower the system pH [31] acidic can bring about alterations in several MFC system parameters such as concentration of ions, membrane potential, proton motive force and adversely affect bacterial growth and adhesion during MFC operation. Raghavulu et al. [80] found that acidophilic pH in anodic chamber was effective performance with respect to power output compared to the corresponding neutral and alkaline operations. However, substrate degradation was observed to be higher in neutral condition followed by alkaline and acidophilic operations. On the other side, though cation exchange membranes (CEM) and anion exchange membranes (AEM) are less expensive separators [72], pH in the cathode chamber increases because of the transport of the cationic species other than protons (particularly of alkali and alkaline earth metals) through the CEM. This increase in pH negatively impacts the performance of the MFC. For every 3 unit increase in pH, a loss of potential of 0.18 V occurs in the cathode, according to [83]. Ideally, the only membranes that can prevent the pH effect on MFC performance are 100% proton selective membrane. Buffers have been commonly used in MFCs in order to (i) maintain a suitable pH for the anodic bacteria biofilm, (ii) increase solution conductivity, (iii) increase the transfer of protons generated at the anode to the cathode, and (iv) reduce the operational pH, which would be beneficial for oxygen reduction. Hence, the primary challenge in scaling-up an MFC application is to ensure a more stable electrolyte environment in the MFC by adding buffers in continuous mode.
3.3.3 Effect of Hydraulic Retention Time (HRT)
HRT is the average amount of time that reactants stay in a reactor and it is calculated by dividing the volume of a reactor by the influent flow rate. Oon et al. [75] studied the effect of HRT on MFC performance and reported that longer HRT allows longer contact time between the substrates and biofilm, which enabled more organic matter to be oxidized by microbes, thus, led to a higher reduction of COD. It was found that the COD removal efficiency at 1 day HRT was 70%, and rose about 7% when MFC operation was extended to 2 days HRT and it also enhanced the azo dye decolorisation and bioelectricity generation.
Oon et al. [75] studied the influence of HRT on the decolorisation and bioelectricity generation can be improved by longer HRT (2 days) and by regulating the external resistance closer to the internal resistance of the MFC system. The low concentration of New Coccine (NC) (25 mg/L) improved 17% of the power density to 20.13 ± 0.37 W/m3. The internal resistance decreased from 50 to 32 Ω. As dye concentration increases, the decolorisation efficiency maintained over 90% (200 mg/L NC), whereas, power density dropped to 10.83 ± 1.21 W/m3. Results showed that both decolorisation and power performances were improved with 2 days HRT. However, the HRT was too high, then the depletion of substrate occurs. Due to mass transfer losses, it increased the overpotential of the system thereby reducing the power output. Li et al. [48] studied the influence of HRT on the power density. The maximum power density reached 552.2 mW/m2, when the HRT was 14.8 h. Increasing HRT from 14.8 to 44.4 h decreased the substrate concentration in the anodic chamber, which increased the open circuit voltage of anode from −431 to −283.8 mV. Longer HRTs reduce the organic loading rate, thereby reducing the cell performance due to the rapid depletion of the substrate, whereas shorter HRTs favor the development of non-exoelectrogenous bacteria, which reduces the CE and electrochemical performance with less effective COD removal, hence optimum HRT is preferred.
3.3.4 Microorganisms in Fuel Cells for Dye Wastewater Treatment
Many dissimilatory metal reducing bacteria possess the ability to transfer electrons directly attracting much attention for the application of MFC in dye degradation. Pure strain such as Proteus hauseri ZMd44 [8] and Pseudomonas aeruginosa [31] has been shown to generate electricity in mediator-less MFC systems and degrade the dyes simultaneously. Different azo dyes such as methyl orange (MO), Congo red, reactive blue 172 (RB), reactive yellow 145, and reactive red 2 were investigated in the Pseudomonas-catalyzed MFC [29], which is capable of producing pyocyanin and several other electron-shuttling compounds. Several isolates such as Geobacter sulfurreducens and Beta Proteobacteria have also been shown to produce energy output without the addition of mediators in MFC and dye removal [13]. In some cases, interestingly it has been found that the decolorized intermediates of azo dyes are redox-active chemical species which itself act as a mediator [8]. An overview of different species of microorganisms employed in the MFC operation with specified dye-bearing substrate. Different species involved in the MFC operation and their possible decolorization efficiency with power output is given in Table 4. Bacteria were identified as the members of the genera Azospirillum, Methylobacterium, Rhodobacter, Desulfovibrio, Trichococcus, and Bacteroides were more abundant in the dye degradation process.
3.3.5 Effect of Electrode
Among all the MFC components, the electrode materials play a crucial role in power generation since the cost of the electrode is a key barrier to the translation of MFCs from the laboratory scale to the industrial process [82]. The anodic material must be conductive, highly porous with enhanced active surface area, noncorrosive, and exhibit redox behavior compatible with microbial metabolism [30]. Different types of materials ranging from non-corrosive stainless steel to versatile carbons have been examined as anodes in different configurations [78]. Similarly, cathode materials also have an equally important effect on MFC operation and productivity. Ideally, cathodes are expected to have a high redox potential to accept electrons [71]. However, it is difficult to obtain high cathodic potentials unless metal-oxide-loaded catalysts are used. [33] employed granular activated carbon as the bioanode and biocathode for the treatment of industrial dye wastewater in an MFC to avoid the platinum catalyst and Nafion membrane, which are two tailbacks in MFC in terms of affordable costs. A stable voltage of 0.214 V was attained with 73% and 77% decolorization at the anode and cathode, respectively. Cui et al. [10] filled a cuboid titanium basket (L 13 cm × W 10 cm × H 7 cm) tightly with granular graphite and welded a titanium rod of 30 cm length on the basket for the external circuit connection. The large specific surface area provided by the graphite granules in this configuration was appreciably better for the power generation. Graphite-epoxy resin composite electrodes (MS-GECE) doped with various metal salts (MS) were tested by Jayapriya and Ramamurthy [31, 32] in dye degradation using Pseudomonas aeruginosa as the biocatalyst. The best-performing MFC was the one with Mn2+ GECE electrodes, producing the highest power density for methyl orange (4676 mW/m2) and reactive black (2593 mW/m2).[96] used a biocathode MFC for Congo red decolorization in textile dyeing sludge. The biocathode MFC exhibited excellent performance, achieving Congo red decolorization rapidly in 48 h, with a power density of 9 mW/m2. Bulk-modified graphite-polyester composite electrodes (GPECE) doped with metal salts (MS-GPECE) prepared by casting [69] displayed redox behavior suitable for bacterial metabolism, and enhanced biofilm formation was observed in a Pseudomonas-catalyzed microbial fuel cell (MFC). The highest power density (1575 ± 223.26 μW/m2) achieved with Ni-GPECE as the cathode material was approximately 15-fold higher than that obtained with graphite block, and its methyl-orange decolorization efficiency was 97 ± 1.4%. Nickel cobaltite (NiCo2O4)- and conductive polyaniline (PANI)-modified carbon cloth (CC) electrodes were synthesized by simple, low-cost hydrothermal and electropolymerization techniques, respectively, by Narayanasamy and Jayaprakash [70]. Among the different electrode combinations tested, the surface-modified electrode Ni/PANI/CC (NCCP) produced the maximum power density when applied as a cathode (12.194 ± 0.59 mW/m2), which was 15.9-fold higher than that obtained with CC (0.769 ± 0.0023 mW/m2), besides a high decolorization efficiency of 80.5 ± 2.1%. The preferred electrodes for dye decolorization seem to be mostly carbon and graphite electrodes. Though the maximum color removal has been achieved with carbon-based electrodes in combination with a Pt-catalyst-modified cathode, the incorporation of carbon nanotubes (CNTs) in conductive polymers has become a promising cost-effective electrode fabrication strategy in the MFC arena [72, 99]. All these efforts aspire to unearth the best electrode combination for effective decolorization of azo dyes while generating power as a by-product in MFCs.
4 Conclusion
If power output in these systems can be increased, MFC technology may provide a novel method to offset the high operating cost of traditional wastewater treatment plants, thereby making water recycling and waste disposal more inexpensive for developing and developed nations. MFCs operated with different synthetic dyes and polycyclic aromatic compounds at the pilot-scale demonstrate feasibility but require technical improvements toward high degradation efficiency and lower cost to be economically competitive at the industrial level. Evident inadequacies in commercialization aspects, including cost and durability, have been detected with respect to some MFC components, such as electrode materials, oxygen-reduction reaction catalysts, and membranes. Nowadays, attention toward the development of alternate materials is mounting, and MFC technology might lay a sophisticated path for the direct management of industrial dye effluents and PAH-rich petroleum waste via bioremediation.
References
Abbasian F, Lockington R, Mallavarapu M, Naidu R (2015) A comprehensive review of aliphatic hydrocarbon biodegradation by bacteria. Appl Biochem Biotechnol 176(3):670–699
Adelaja O, Keshavarz T, Kyazze G (2014) Enhanced biodegradation of phenanthrene using different inoculum types in a microbial fuel cell. Eng Life Sci 14(2):218–228
Adelaja O, Keshavarz T, Kyazze G (2015) The effect of salinity, redox mediators and temperature on anaerobic biodegradation of petroleum hydrocarbons in microbial fuel cells. J Hazard Mater 283:211–217
Bregnard T-A, Höhener P, Zeyer J (1998) Bioavailability and biodegradation of weathered diesel fuel in aquifer material under denitrifying conditions. Environ Toxicol Chem 17(7):1222–1229
Buitrón G, Quezada M, Moreno G (2004) Aerobic degradation of the azo dye acid red 151 in a sequencing batch biofilter. Biores Technol 92(2):143–149
Cao Y, Yongyou H, Sun J, Hou B (2010) Explore various co-substrates for simultaneous electricity generation and congo red degradation in air-cathode single-chamber microbial fuel cell. Bioelectrochemistry 79(1):71–76
Chandrasekhar K, Venkata Mohan S (2012) Bio-electrochemical remediation of real field petroleum sludge as an electron donor with simultaneous power generation facilitates biotransformation of PAH: effect of substrate concentration. Biores Technol 110:517–525
Chen BY et al (2010) Assessment upon Azo dye decolorization and bioelectricity generation by proteus hauseri. Biores Technol 101(12):4737–4741
Coelho A, Castro AV, Dezotti M, Sant’Anna GL (2006) Treatment of petroleum refinery sourwater by advanced oxidation processes. J Hazard Mater 137(1):178–184
Cui D et al (2014) Enhanced decolorization of azo dye in a small pilot-scale anaerobic baffled reactor coupled with biocatalyzed electrolysis system (ABR-BES): a design suitable for scaling-up. Biores Technol 163:254–261
Dai Q et al (2020) Sulfide-mediated azo dye degradation and microbial community analysis in a single-chamber air-cathode microbial fuel cell. In: Bioelectrochemistry
Environmental Engineering (2012) Microbial degradation of xenobiotics. http://www.springerlink.com/index/10.1007/978-3-642-23789-8
Fang Z, Song HL, Cang N, Li XN (2013) Performance of microbial fuel cell coupled constructed wetland system for decolorization of azo dye and bioelectricity generation. Biores Technol 144:165–171
Fang Z, Song HL, Cang N, Li XN (2015) Electricity production from Azo dye wastewater using a microbial fuel cell coupled constructed wetland operating under different operating conditions. Biosens Bioelectron 68:135–141
Fang Z, Song H, Yu R, Li X (2016) A microbial fuel cell-coupled constructed wetland promotes degradation of azo dye decolorization products. Ecol Eng 94:455–463
Fang Z, Cao X, Li X, Wang H, Li X (2017) Electrode and azo dye decolorization performance in microbial-fuel-cellcoupled constructed wetlands with different electrode size during long-term wastewater treatment. Bioresour Technol 238: 450–460
Fu L et al (2019) Ozonation reactivity characteristics of dissolved organic matter in secondary petrochemical wastewater by single ozone, ozone/H2O2, and ozone/catalyst. Chemosphere 233:34–43
Ganesh R, Boardman GD, Michelsen D (1994) Fate of azo dyes in sludges. Water Res 28(6):1367–1376
Giannopoulos D et al (2007) Quantification of emissions from the co-incineration of cutting oil emulsions in cement plants—Part II: trace species. Fuel 86(16):2491–2501
Goyal RN, Minocha A (1985) Electrochemical behaviour of the bisazo dye, direct red-81. J Electro Chem 193(1–2):231–240
Guo X et al (2014) The influence of microbial synergistic and antagonistic effects on the performance of refinery wastewater microbial fuel cells. J Power Sour 251:229–236
Hassan H et al (2018) Microbial community and bioelectrochemical activities in MFC for degrading phenol and producing electricity: microbial consortia could make differences. Chem Eng J 332:647–657
Holzman DC (2005) Microbe power!. Environ Health Perspect 113(11)
Hosseinpour M et al (2016) Ethylene glycol biodegradation in microbial fuel cell. Energy Sour Part A: Recov Utiliz Environ Effects 38(8):1096–1102
Huang DY et al (2011) Enhanced anaerobic degradation of organic pollutants in a soil microbial fuel cell. Chem Eng J 172(2–3):647–653
Huang L et al (2011) Degradation of pentachlorophenol with the presence of fermentable and non-fermentable co-substrates in a microbial fuel cell. Biores Technol 102(19):8762–8768
Illamathi J (2017) Microbial fuel cells for dye decolorization. Environ Chem Lett 16(10)
Jang JK et al (2004) Construction and operation of a novel mediator- and membrane-less microbial fuel cell. Process Biochem 39(8):1007–1012
Jayaprakash J, Parthasarathy A, Viraraghavan R (2016) Decolorization and degradation of monoazo and diazo dyes in pseudomonas catalyzed microbial fuel cell. Environ Prog Sustain Energy 35(6):1623–1628
Jayapriya J et al (2012) Preparation and characterization of biocompatible carbon electrodes. Compos B Eng 43(3):1329–1335
Jayapriya J, Ramamurthy V (2012) Use of non-native phenazines to improve the performance of pseudomonas aeruginosa MTCC 2474 catalysed fuel cells. Biores Technol 124:23–28
Jayapriya J, Ramamurthy V (2014) The role of electrode material in capturing power generated in pseudomonas catalysed fuel cells. Can J Chem Eng 92(4):610–614
Kalathil S, Lee J, Cho MH (2011) Granular activated carbon based microbial fuel cell for simultaneous decolorization of real dye wastewater and electricity generation. New Biotechnol 29(1):32–37
Kaushik A, Singh A (2020) Metal removal and recovery using bioelectrochemical technology: the major determinants and opportunities for synchronic wastewater treatment and energy production. J Environ Manag, 270
Khalid A, Arshad M, Crowley DE (2009) Biodegradation potential of pure and mixed bacterial cultures for removal of 4-nitroaniline from textile dye wastewater. Water Res 43(4):1110–1116
Khalid S, Alvi F, Fatima M, Aslam M, Riaz S, Farooq R, Zhang Y (2018) Dye degradation and electricity generation using microbial fuel cell with graphene oxide modified anode. Mater Lett 220:272–276
Krithika T, Kavitha R, Dinesh M, Angayarkanni J (2021) Assessment of ligninolytic bacterial consortium for the degradation of azo dye with electricity generation in a dual-chambered microbial fuel cell. Environ Challenges 4
Kulkarni SV et al (1985) Textile dyes and dyeing equipment: classification, properties, and environmental aspects, i
Kumari S, Regar RK, Manickam N (2018) Improved polycyclic aromatic hydrocarbon degradation in a crude oil by individual and a consortium of bacteria. Biores Technol 254:174–179
Lai CY, Wu CH, Meng CT, Lin CW (2017) Decolorization of azo dye and generation of electricity by microbial fuel cell with laccase-producing white-rot fungus on cathode. Appl Energy 188:392–398
Li X et al (2014) Extended petroleum hydrocarbon bioremediation in saline soil using Pt-free multianodes microbial fuel cells. RSC Adv 4(104):59803–59808
Li X et al (2015) Sand Amendment enhances bioelectrochemical remediation of petroleum hydrocarbon contaminated soil. Chemosphere 141:62–70
Li X, Wang X, Zhao Q et al (2016) Carbon fiber enhanced bioelectricity generation in soil microbial fuel cells. Biosens Bioelectron 85:135–141
Li X, Wang X, Wan L et al (2016) Enhanced biodegradation of aged petroleum hydrocarbons in soils by glucose addition in microbial fuel cells. J Chem Technol Biotechnol 91(1):267–275
Li X, Wang X, Zhang Y et al (2016) Salinity and conductivity amendment of soil enhanced the bioelectrochemical degradation of petroleum hydrocarbons. Sci Rep 6(September):1–11
Li X et al (2018) Surfactants selectively reallocated the bacterial distribution in soil bioelectrochemical remediation of petroleum hydrocarbons. J Hazard Mater 344:23–32
Li X, Li Y, Zhang X et al (2019) Long-term effect of biochar amendment on the biodegradation of petroleum hydrocarbons in soil microbial fuel cells. Sci Total Environ 651:796–806
Li Z et al (2010) Azo dye treatment with simultaneous electricity production in an anaerobic-aerobic sequential reactor and microbial fuel cell coupled system. Biores Technol 101(12):4440–4445
Liang S, Zhang B, Shi J, Wang T, Zhang L, Wang Z, Chen C (2018) Improved decolorization of dye wastewater in an electrochemical system powered by microbial fuel cells and intensified by micro-electrolysis. Bioelectrochemistry 124:112–118
Liu XW et al (2011) Integration of a microbial fuel cell with activated sludge process for energy-saving wastewater treatment: taking a sequencing batch reactor as an example. Biotechnol Bioeng 108(6):1260–1267
Lovley DR (1993) Dissimilatory metal reduction. Annu Rev Microbiol 47:263–290
Lovley DR, Woodward JC, Chapelle FH (1996) Rapid anaerobic benzene oxidation with a variety of chelated Fe(III) forms. Appl Environ Microbiol 62(1):288–291
Lu L et al (2014) Microbial metabolism and community structure in response to bioelectrochemically enhanced remediation of petroleum hydrocarbon-contaminated soil. Environ Sci Technol 48(7):4021–4029
Maier J et al (2004) A New Alkali-thermostable azoreductase from bacillus sp. Strain SF. Appl Environ Microbiol 70(2):837–844
Majumder D et al (2014) Electricity generation and wastewater treatment of oil refinery in microbial fuel cells using pseudomonas putida. Int J Mol Sci 15(9):16772–16786
Menek N, Zeyrekl S, Karaman Y, Investigation of electrochemical behavior of mordant dye ( C. I. 17135 ) at glassy carbon and silver electrodes 4(1):89–104
Miran W et al (2016) Simultaneous electricity production and direct red 80 degradation using a dual chamber microbial fuel cell. Desalin Water Treat 57(19):9051–9059
Mohammadi L et al (2020) Petroleum hydrocarbon removal fromwastewaters: a review. Processes 8(4):1–34
Mohan SV, Chandrasekhar K (2011) Self-induced bio-potential and graphite electron accepting conditions enhances petroleum sludge degradation in bio-electrochemical system with simultaneous power generation. Biores Technol 102(20):9532–9541
Mohanakrishna G et al (2018) Enhanced treatment of petroleum refinery wastewater by short-term applied voltage in single chamber microbial fuel cell. Biores Technol 253:16–21
Mohanakrishna G, Abu-Reesh IM, Al-Raoush RI (2018) Biological anodic oxidation and cathodic reduction reactions for improved bioelectrochemical treatment of petroleum refinery wastewater. J Clean Prod 190:44–52
Mohanakrishna G, Al-Raoush RI, Abu-Reesh IM (2018) Induced bioelectrochemical metabolism for bioremediation of petroleum refinery wastewater: optimization of applied potential and flow of wastewater. Biores Technol 260:227–232
Mohanakrishna G, Al-Raoush RI, Abu-Reesh IM (2020) Sewage enhanced bioelectrochemical degradation of petroleum hydrocarbons in soil environment through bioelectro-stimulation. Biotechnol Rep, 27
Mohanakrishna G, Al-Raoush RI, Abu-Reesh IM, Aljaml K (2019) Removal of petroleum hydrocarbons and sulfates from produced water using different bioelectrochemical reactor configurations. Sci Total Environ 665:820–827
Mohanakrishna G, Al-Raoush RI, Abu-Reesh IM, Pant D (2019) A microbial fuel cell configured for the remediation of recalcitrant pollutants in soil environment. RSC Adv 9(71):41409–41418
Morris JM, Jin S (2008) Feasibility of using microbial fuel cell technology for bioremediation of hydrocarbons in groundwater. J Environ Sci Health—Part A Toxic/Hazard Subst Environ Eng 43(1):18–23
Morris JM, Jin S (2012) Enhanced biodegradation of hydrocarbon-contaminated sediments using microbial fuel cells. J Hazard Mater 213–214:474–477
Mu W et al (2009) 3-Phenyllactic acid production by substrate feeding and PH-control in fed-batch fermentation of lactobacillus Sp. SK007. Biores Technol 100(21):5226–5229
Narayanasamy S, Jayaprakash J (2018) Improved performance of pseudomonas aeruginosa catalyzed MFCs with graphite/polyester composite electrodes doped with metal ions for azo dye degradation. Chem Eng J 343:258–269
Narayanasamy S, Jayaprakash J (2021a) Carbon cloth/nickel cobaltite (NiCo2O4)/polyaniline (PANI) composite electrodes: preparation, characterization, and application in microbial fuel cells. Fuel, 301
Narayanasamy S, Jayaprakash J (2021b) Conducting polymers for electrocatalysts. In: Conducting polymers for advanced energy applications, pp 315–335
Navanietha KR, Yu JS (2015) Bioenergy: opportunities and challenges. In: Bioenergy: opportunities and challenges
Noonpui S, Thiravetyan P (2011) Treatment of reactive azo dye from textile wastewater by burhead (Echinodorus Cordifolius L.) in constructed wetland: effect of molecular size. J Environ Sci Health—Part A Toxic/Hazard Subst Environ Eng 46(7):709–714
O’Neill C et al (1999) Colour in textile effluents—Sources, measurement, discharge consents and simulation: a review. J Chem Technol Biotechnol 74(11):1009–1018
Oon YS et al (2018) Disclosing the synergistic mechanisms of azo dye degradation and bioelectricity generation in a microbial fuel cell. Chem Eng J 344:236–245
Oon YS, Ong SA, Ho LN, Wong YS, Oon YL, Lehl HK, Thung WE, Nordin N (2017) Microbial fuel cell operation using monoazo and diazo dyes as terminal electron acceptor for simultaneous decolourisation and bioelectricity generation. J Hazard Mater 325:170–177
Oon YL, Ong SA, Ho LN, Wong YS, Dahalan FA, Oon YS, Teoh TP, Lehl HK, Thung WE (2020) Constructed wetland–microbial fuel cell for azo dyes degradation and energy recovery: influence of molecular structure, kinetics, mechanisms and degradation pathways. Sci Total Environ 720
Parthasarathy P, Narayanan SK (2014) Effect of hydrothermal carbonization reaction parameters on. Environ Prog Sustain Energy 33(3):676–680
Priyadarshini M, Ahmad A, Das S, Ghangrekar MM (2021) Application of microbial electrochemical technologies for the treatment of petrochemical wastewater with concomitant valuable recovery: a review. Environ Sci Pollut Res
Raghavulu SV, Mohan SV, Goud RK, Sarma PN (2009) Effect of anodic PH microenvironment on microbial fuel cell (MFC) performance in concurrence with aerated and ferricyanide catholytes. Electrochem Commun 11(2):371–375
Rajaguru P et al (2002) Genotoxicity evaluation of polluted ground water in human peripheral blood lymphocytes using the comet assay. Mutat Res—Genet Toxicol Environ Mutagen 517(1–2):29–37
Rene ER, Shu L, Jegatheesan V (2019) Editorial: sustainable Eco-technologies for water and wastewater treatment. J Water Supply: Res Technol—AQUA 68(8):617–622
Rozendal RA, Hamelers HVM, Buisman CJN (2006) Effects of membrane cation transport on PH and microbial fuel cell performance. Environ Sci Technol 40(17):5206–5211
Rueter P et al (1994) Anaerobic oxidation of hydrocarbons in crude oil by new types of sulphate-reducing bacteria. Nature 372(6505):455–458
dos Santos AB, Cervantes FJ, van Lier JB (2007) Review paper on current technologies for decolourisation of textile wastewaters: perspectives for anaerobic biotechnology. Biores Technol 98(12):2369–2385
Sarma MK, Quadir MGA, Bhaduri R, Kaushik S, Goswami P (2018) Composite polymer coated magnetic nanoparticles based anode enhances dye degradation and power production in microbial fuel cells. Biosens Bioelectron 119:94–102
Sarmin S et al (2019) Bio-electrochemical power generation in petrochemical wastewater fed microbial fuel cell. Sci Total Environ, 695
Sevda S, Abu-Reesh IM (2019) Improved petroleum refinery wastewater treatment and seawater desalination performance by combining osmotic microbial fuel cell and up-flow microbial desalination cell. Environ Technol (United Kingdom) 40(7):888–895
Shah S, Venkatramanan V, Prasad R (2019) Microbial fuel cell: sustainable green technology for bioelectricity generation and wastewater treatment. In: Sustainable green technologies for environmental management, pp 199–218
Sharma M et al (2020) Bioelectrochemical remediation of phenanthrene in a microbial fuel cell using an anaerobic consortium enriched from a hydrocarbon-contaminated site. J Hazard Mater, 389
Sherafatmand M, Ng HY (2015) Using sediment microbial fuel cells (SMFCs) for bioremediation of polycyclic aromatic hydrocarbons (PAHs). Biores Technol 195:122–130
Shim H, Yang ST (2002) BTEX removal from contaminated groundwater by a co-culture of Pseudomonas putida and Pseudomonas fluorescens immobilized in a continuous fibrous-bed bioreactor. J Chem Technol Biotechnol 77(12):1308–1315
Singh JG, Chang-Yen I, Stoute VA, Chatergoon L (1992) Hydrocarbon levels in edible fish, crabs and mussels from the marine environment of Trinidad. Mar Pollut Bull 24(5):270–272
Srikanth S et al (2016) Electro-biocatalytic treatment of petroleum refinery wastewater using microbial fuel cell (MFC) in continuous mode operation. Biores Technol 221:70–77
Sun J et al (2011) Further treatment of decolorization liquid of azo dye coupled with increased power production using microbial fuel cell equipped with an aerobic biocathode. Water Res 45(1):283–291
Sun J et al (2016) Regulation of biocathode microbial fuel cell performance with respect to azo dye degradation and electricity generation via the selection of anodic inoculum. Int J Hydrog Energy 41(9):5141–5150
Sun J, Hu YY, Bi Z, Cao YQ (2009) Simultaneous decolorization of azo dye and bioelectricity generation using a microfiltration membrane air-cathode single-chamber microbial fuel cell. Bioresour Technol 100(13):3185–3192
Tacas ACJ, Tsai PW, Tayo LL, Hsueh CC, Sun SY, Chen BY (2021) Degradation and biotoxicity of azo dyes using indigenous bacteria-acclimated microbial fuel cells (MFCs). Process Biochem 102: 59–71
Tan SP et al (2017) Treatment of palm oil mill effluent using combination system of microbial fuel cell and anaerobic membrane bioreactor. Biores Technol 245:916–924
Thung WE et al (2015) A highly efficient single chambered up-flow membrane-less microbial fuel cell for treatment of azo dye acid orange 7-containing wastewater. Biores Technol 197:284–288
Tian X et al (2020) A comprehensive review on toxic petrochemical wastewater pretreatment and advanced treatment. J Clean Prod, 245
Varjani SJ, Upasani VN (2016) Carbon spectrum utilization by an indigenous strain of Pseudomonas aeruginosa NCIM 5514: production, characterization and surface active properties of biosurfactant. Biores Technol 221:510–516
Vasudevan N, Rajaram P (2001) Bioremediation of oil sludge-contaminated soil. Environ Int 26(5–6):409–411
Wang S et al (2021) Advances in research on petroleum biodegradability in soil. Environ Sci Process Impacts 23(1):9–27
Wang X et al (2012) Bioelectrochemical stimulation of petroleum hydrocarbon degradation in saline soil using U-tube microbial fuel cells. Biotechnol Bioeng 109(2):426–433
Xu H, Quan X, Xiao Z, Chen L (2017) Cathode modification with peptide nanotubes (PNTs) incorporating redox mediators for azo dyes decolorization enhancement in microbial fuel cells. Int J Hydrogen Energy 42(12):8207–8215
Xu H, Quan X, Chen L (2019) A novel combination of bioelectrochemical system with peroxymonosulfate oxidation for enhanced azo dye degradation and MnFe2O4 catalyst regeneration. Chemosphere 217:800–807
Yadav AK, Dash P, Mohanty A, Abbassi R, Mishra BK (2012) Performance assessment of innovative constructed wetland-microbial fuel cell for electricity production and dye removal. Ecol Eng 47:126–131
Yan Z et al (2012) Enhanced degradation of phenanthrene and pyrene in freshwater sediments by combined employment of sediment microbial fuel cell and amorphous ferric hydroxide. J Hazard Mater 199–200:217–225
Yan Z et al (2015) Complex interactions between the macrophyte acorus calamus and microbial fuel cells during pyrene and benzo[a]pyrene degradation in sediments. Sci Rep 5(December 2014):1–11
Yang Y et al (2015) Enhancing the bioremediation by harvesting electricity from the heavily contaminated sediments. Biores Technol 179:615–618
Yeruva DK et al (2015) Integrating sequencing batch reactor with bio-electrochemical treatment for augmenting remediation efficiency of complex petrochemical wastewater. Biores Technol 188:33–42
Yuan Y, Zhou S, Zhuang L (2010) A new approach to in situ sediment remediation based on air-cathode microbial fuel cells. J Soils Sediments 10(7):1427–1433
Zhang C, Liu G, Zhang R, Luo H (2010) Electricity production from and biodegradation of quinoline in the microbial fuel cell. J Environ Sci Health—Part A Toxic/Hazard Substan Environ Eng 45(2):250–256
Zhang Q et al (2012) Deciphering effects of chemical structure on azo dye decolorization/degradation characteristics: bacterial versus photocatalytic method. J Taiwan Inst Chem Eng 43(5):760–766
Zhang T et al (2010) Stimulating the anaerobic degradation of aromatic hydrocarbons in contaminated sediments by providing an electrode as the electron acceptor. Environ Microbiol 12(4):1011–1020
Zhang Y et al (2015) Horizontal arrangement of anodes of microbial fuel cells enhances remediation of petroleum hydrocarbon-contaminated soil. Environ Sci Pollut Res 22(3):2335–2341
Zhao L et al (2019) Investigation of PAH and oil degradation along with electricity generation in soil using an enhanced plant-microbial fuel cell. J Clean Prod 221:678–683
Zhong D, Liu Y, Liao X, Zhong N, Xu Y (2018) Facile preparation of binder-free NiO/MnO2-carbon felt anode to enhance electricity generation and dye wastewater degradation performances of microbial fuel cell. Int J Hydrogen Energy 43(51):23014–23026
Zhou L et al (2016) Microbial electricity generation and isolation of exoelectrogenic bacteria based on petroleum hydrocarbon-contaminated soil. Electroanalysis 28(7):1510–1516
Zsirai T et al (2018) Cleaning of ceramic membranes for produced water filtration. J Petrol Sci Eng 166:283–289
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Sujatha, R.M., Mary, L.M., Jayapriya, J. (2022). Bioremediation of Petrochemicals and Dye Industrial Effluents through Microbial Fuel Cells. In: Ahmad, A., Mohamad Ibrahim, M.N., Yaqoob, A.A., Mohd Setapar, S.H. (eds) Microbial Fuel Cells for Environmental Remediation. Sustainable Materials and Technology. Springer, Singapore. https://doi.org/10.1007/978-981-19-2681-5_12
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