Abstract
Among the prevalent tissue culture techniques of agricultural and horticultural promise, meristem and shoot tip culture has been exploited at a much wider scale primarily due to its application in diverse areas such as rapid clonal multiplication of vegetatively propagated crop plants, virus elimination and germplasm preservation of both vegetatively and seed propagated crops (Kartha, 1981; 1986; George and Sherrington, 1984; Hussey, 1986), and more recently in the development of genetic transformation protocols for crop improvement through gene transfer (Ulian, 1988; Gould et al., 1991).
NRCC Publication No. 32483.
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Adams, A.N. (1972). An improved medium for strawberry meristem culture. J. Hort. Sci. 47: 263–264.
Adejare, G.O. and Coutts, R.H.A. (1981). Eradication of cassava mosaic disease from Nigerian cassava (Manihot esculenta) clones by meristem tip culture. Plant Cell Tissue Org. Cult. 1, 25–32.
Ahmed, R., Gupta, S.D. and Ghosh, P.D. (1987). The cytological status of plants regenerated from shoot meristem culture of Pisum sativum L. Plant Breeding 98: 306–311.
Aitken-Christie, J. (1991). Automation. In: Micropropagation–Technology and Application. P.C. Debergh and R.H. Zimmerman (eds.), Kluwer Academic Publishers, Dordrecht, pp. 363–388.
Aitken-Christie, J. and Singh, A.P. (1987). Cold storage of tissue cultures. In: Tissue Culture in Forestry. J. Bonga, and D. Durzan (eds.), Martinus Nijhoff Publishers, The Hague.
Anderson, W.C. and Carstens J.B. (1977). Tissue culture propagation of broccoli, Brassica oleracea (Italica group), for use in F1 hybrid seed production. J. Am. Soc. Hort. Sci. 102: 69–73.
Anderson, H.M., Abbott, A.J. and Wiltshire, S. (1982). Micropropagation of strawberry plants in vitro - Effect of growth regulators on incidence of multi-apex abnormality. Sci. Hort. 16: 331–341.
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J G., Smith, J.A. and Struhl, K. (1989). Current Protocols in Molecular Biology, Vol. 1. Greene Publishing, New York.
Ball, E. (1946). Development of sterile cultures of stem tips and adjacent regions of Tropaeolum majus L. and Lupinus majus L. Am. J. Bot. 33: 301–318.
Banerjee, N. 1 and De Langhe, E. (1985). A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (banana and plantain). Plant Cell Rep. 4: 351–354.
Baraldi, R., Rossi, F. and Lercari, B. (1988). In vitro shoot development of Prunus GF665–2: interaction between light and benzyladenine. Physiol. Plant. 74:440–443.
Barlass, M. and Skene, K.G.M. (1986). Citrus (Citrus species). In: Biotechnology in Agriculture and Forestry, Vol. 1: Trees. Y.P.S. Bajaj (ed.), Springer-Verlag, Heidelberg, pp. 207–219.
Baumann, G., Casper, R. and Kornkamhaeng, P. (1984). Detection of prune dwarf virus by ELISA in meristem-propagated sour cherry plants during in vitro culture. Phytopath. Z. 110: 168–171.
Belles, J.M., Hansen, A.J., Granell, A. and Conejero, V. (1986). Antiviroid effects of ribavirin on citrus exocortis viroid infection in Gynura aurantiaca DC. Physiol Mol. Plant Pathol. 28: 61–65.
Bhagyalakshmi and Singh, N.S. (1988). Meristem culture and micropropagation of a variety of ginger (Zingiber officinale Rose.) with a high yield of oleoresin. J. Hort. Sci. 63: 321–327.
Boxus, P. (1974). The production of strawberry plants by in vitro micropropagation. J. Hort. Sci. 49: 209–210.
Boxus, P., Quoirin, M. and Laine, J.M. (1977). Large scale propagation of strawberry plants from tissue culture. In: Applied and Fundamental Aspects of Plant Cell Tissue and Organ Culture. J. Reinert and Y.P.S Bajaj (eds.), Springer-Verlag, New York, pp. 130–143.
Brown, C.R., Kwiatkowski, S., Martin, M.W. and Thomas, P.E. (1988). Eradication of PVS from potato clones through excision of meristems from in vitro, heat treated shoot tips. Am. Potato J. 65: 633–638.
Cassells, A.C. (1987). In vitro induction of virus-free potatoes by chemotherapy. In: Biotechnology in Agriculture and Forestry, Vol. 3: Potatoes. Y.P.S. Bajaj (ed.), Springer-Verlag, New York, pp. 40–50.
Cassells, A.C. and Long, R.D. (1980). The regeneration of virus-free plants from cucumber mosaic virus and potato virus Y infected tobacco explant cultures in the presence of virazole. Z. Naturf 35: 350–351.
Cassells, A.C. and Long, R.D. (1982). The elimination of potato virus X, Y, S, and M in meristem and explant cultures of potato in the presence of virazole. Potato Res. 25: 165–173.
Chong, C. and Pua, E.C. (1985). Carbon nutrition of Ottawa 3 apple rootstock during stages of in vitro propagation. J. Hort. Sci. 60: 285–290.
Converse, R.H. and Tanne, E. (1984). Heat therapy and stolon apex culture to eliminate mild yellow-edge virus from Hood strawberry. Phytopathology 74: 1315–1316.
Cronauer, S.S. and Krikorian, A.D. (1984). Rapid multiplication of bananas and plantains by in vitro shoot tip culture. Hort. Sc. 19: 234–235.
Dawson, W.O. (1976). Synthesis of TMV RNA at restrictive high temperatures. Virology 73: 319–326.
Debergh, P.C. and Zimmerman, R.H. (1991). Micropropagation - Technology and Application. Kluwer Academic Publishers, Dordrecht.
De Bry (1986). Robots in plant tissue culture: an insight. IAPTC News Lett. 49: 2–22.
Donnan, A. (1986). Determining and minimizing production costs. In: Tissue Culture as a Plant Production System for Horticultural crops. R.H. Zimmerman, R.H. Griesbach, R.A. Hammerschlag and R.H. Lawson, (eds.), Martinus Nijhoff Publishers, Dordrecht, pp. 167–173.
DoreSwamy, R., Srinivasa Rao, N.K. and Chacko, E.K. (1982). Tissue culture propagation of banana. Sci. Hort. 18: 247–252.
Drew, R.A. (1987). The effect of media composition and cultural conditions on in vitro root initiation and growth of papaya (Carica papaya L.). J. Hort. Sci. 62: 551–556.
Dunstan, D.I. and Short, K.C. (1977). Improved growth of tissue culture of the onion, Allium cepa. Physiol. Plant. 41: 70–72.
Etienne, L., Clauzel, J.M. and Fuchs, M. (1991). Simultaneous detection of several nepoviruses infecting grapevine in a single DAS-ELISA test using mixed antisera. J. Phytopathology 131: 89–100.
Fraley, R.T., Horsch, R.B. and Matzke, A. (1984). In vitro transformation of Petunia cells by an improved method of co-cultivation with A. tumefaciens strains. Plant Mol. Biol. 3:371–378.
Fukai, S. and 0e, M. (1990). Morphological observations of chrysanthemum shoot tips cultured after cryoprotection and freezing. J. Japan Soc. Hort. Sci. 59: 383–387.
Fuchs, M., Pinck, M., Etienne, L., Pinck, L. and Walter, B. (1991). Characterization and detection of grapevine fan leaf virus by using cDNA probes. Phytopathology 81: 559–565.
Gallenberg, D.J. and Jones, E.D. (1985). Detection of potato virus X and S in tissue culture plantlets. Am. Potato J. 62: 111–118.
Gamborg, O.L., Miller, R.A. and Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Expt. Cell Res. 50: 151–158.
Gasser, C.S. and Fraley, R.T. (1989). Genetically engineering plants for crop improvement. Science 244: 1293–1299.
Gavinlertvatana, P. and Prutpongse, P. (1991). Commercial micropropagation in Asia. In: Micropropagation–Technology and Application. P.C. Debergh and R.H. Zimmerman (eds.), Kluwer Academic Publishers, Dordrecht, pp. 181–190.
George, E.F. and Sherrington, P.D. (1984). Plant Propagation by Tissue Culture - Handbook and Directory of Commercial Laboratories. Eastern Press, Reading.
Gould, J., Devey, M., Hasegawa, 0., Ulian, E.C., Peterson, G. and Smith, R.H. (1991). Transformation of Zea mays L. using Agrobacterium tumefaciens and the shoot apex. Plant Physiol. 95: 426–434.
Green, S.K. and Lo, C.Y. (1989). Elimination of sweet potato yellow dwarf virus (SPYDV) by meristem tip culture and by heat treatment. J. Plant Dis. Protection 96: 464–469.
Greno, V., Cambra, M., Navarro, L. and Duran-Vila, N. (1990). Effect of antiviral chemicals on the development of virus content of citrus buds cultured in vitro. Scientia Hort. 45: 75–87.
Gupta, P.P. (1986). Eradication of mosaic disease and rapid clonal multiplication of bananas and plantains through meristem tip culture. Plant Cell Tissue Org. Cult. 6: 33–40.
Gupta, P.K., Mascarenhas, A.F. and Jagannathan, V. (1981). Tissue culture of forest trees–clonal propagation of mature trees of Eucalyptus citriodora Hook. by tissue culture. Plant Sci. Lett. 20: 195–201.
Hakkaart, F.A. and Versluijs, J.M.A. (1988). Virus elimination by meristem-tip culture from a range of Alstroemeria cultivars. Neth. J. Plant Path. 94: 49–56.
Hammerschlag, F. (1982). Factors affecting establishment and growth of peach shoots in vitro. Hort. Sc. 17: 85–86.
Hansen, A.J. (1984). Effect of ribavirin on green ring mottle causal agent and necrotic ring spot virus in Prunus species. Plant Dis. 68: 216–218.
Hansen, A.J. (1988). Chemotherapy of plant virus infections. Applied Virology Res. 1: 285–299.
Hansen, A.J. and Lane, W.D. (1985). Elimination of apple chlorotic leaf spot virus from apple shoot cultures by ribavirin. Plant Dis. 69: 134–135.
Hasegawa, P.M. (1979). In vitro propagation of rose. Hort. Sc. 14:610–612.
Hasegawa, P.M. (1980). Factors affecting shoot and root initiation from cultured rose shoot tips. J. Am. Soc. Hort. Sci. 105: 216–220.
Haskins, R.H. and Kartha, K.K. (1980). Freeze-preservation of pea meristems: Cell survival. Can J. Bot. 58: 833–840.
Havel, L. and Novak, F.J: (1985). Meristem tip culture of Allium cepa L. Sci. Hort. 27: 209–214.
Heller, R. (1953). Recherches sui la nutrition minerale des tissus vegetaux cultives in vitro. Ann. Sci. Natl. Biol. Veg. 36: 31–38.
Hildebrandt, V. and Harney, P.M. (1988). Factors affecting the release of phenolic exudate from explants of Pelargonium x hortorum, Bailey `Sprinter Scarlet’. J. Hort. Sci. 63: 651–657.
Hoekema, A., Huisman, M.J., Molendijk, L., Van den-Elzen, P.J.M. and Cornelissen, B.J.C. (1989). The genetic engindering of two commercial potato cultivars for resistance to potato virus X. Bio/Technology 7: 273–278.
Hollings, M. and Stone, O.M. (1968). Techniques and problems in the production of virus-tested planting material. Sci. Hort. 20: 57–72.
Horsch, R.B., Fry, S.E., Hoffman, N.L., Eicholtz, D., Rogers, S.G. and Fraley, R.T. (1985). A simple and general method for transferring genes into plants. Science 227: 1229–1231.
Hu, C.Y. and Wang, P.J. (1983). Meristem shoot tip and bud cultures. In: Handbook of Plant Cell Cultures. A. Evans, W.R. Sharp, P.V. Ammirato and Y. Yamada (eds.), Vol. 1. Macmillan, New York, p. 177–227.
Hughes, K.W. (1981). In vitro ecology: exogenous factors affecting growth and morphogenesis in plant culture systems. Env. Expt. Bot. 21:281–288.
Hunter, S.A., Foxe, M.J. and Hennerty, M.J. (1983). The influence of temperature and light intensity on the in vitro propagation of strawberry (Fragaria x ananassa Duch.) cv. Cambridge Favourites. Acta Hort. 131: 153–161.
Hunter, S.A., Hannon, M., Foxe, M.J. and Hennerty, M.J. (1984). Factors affecting the in vitro production of strawberry (Fragaria x ananassa) meristems. cv. Cambridge Favourite. J. Life Sci. R. Dubl. Soc. 5:13–19.
Hussey, G. (1986). Vegetative propagation of plants by tissue culture. In: Plant Cell Culture Technology, M.M. Yeoman (ed.), Botanical Monographs, Vol. 23. Blackwell Scientific Publications, Oxford, pp. 29–66.
Hussey, G., Johnson, R.D. and Warren, S. (1989). Transformation of meristematic cells in the shoot apex of cultured pea shoots by Agrobacterium tumefaciens and A. rhizogenes. Protoplasma 148: 101–105.
Hyndman, S E., Hasegawa, P.M. and Bressan, R.N. (1982). Stimulation of root initiation from cultured rose shoots through the use of reduced concentrations of mineral salts. Hort. Sc. 17: 82–83.
Jaiswal, V.S. and Amin, M.N. (1987). In vitro propagation of guava from shoot cultures of mature trees. J. Plant Physiol. 130:7–12.
James, D.J and Newton, B. (1977). Auxin: cytokinin interactions in the in vitro micropropagation of strawberry. Acta Hort. 78: 321–331.
Janeckova, M. (1988). The raspberry virus elimination program in Czechoslovakia. Acta Hort. 236: 45–50.
Jones, O:P., Zimmerman, R.H., Fordham, I.M. and Hopgood, M.E. (1985). Propagation in vitro of some dwarf apple trees. J. Hort. Sci. 60: 141–144.
Kallak, H. and Koiveer, A. (1990). Induction of morphogenesis in meristems of different cultivars of Pisum sativum L. Plant Sci. 67: 221–226.
Kanaiewski, W.K. and Thomas, P.E. (1988). A two step ELISA for rapid, reliable detection of potato viruses. Am. Potato J. 65: 561–571.
Karp, A. (1989). Can genetic instability be controlled in plant tissue cultures? IAPTC News Lett. 58: 2–11.
Kartha, K.K. (1981). Meristem culture and cryopreservation–methods and applications. In: Plant Tissue Culture–Methods and Applications. T.A. Thorpe (ed.), Academic Press, New York, pp. 118–211.
Kartha, K.K. (1984a). Culture of shoot apical meristems. In: Cell Culture and Somatic Cell Genetics of Plants, Vol. 1. I.K. Vasil (ed.), Academic Press, New York, pp. 106–110.
Kartha, K.K. (1984b). Tissue culture techniques for virus elimination and germplasm preservation. In: Plant Breeding Reviews, Vol. 2. J. Janick (ed.), AVI Publishing Co., Connecticut, pp. 265–279.
Kartha, K.K. (1985a). Cryopreservation of Plant Cells and Organs. CRC Press Inc., Boca Raton, Florida.
Kartha, K.K. (1985b). Meristem culture and germplasm preservation. In: Cryopreservation of Plant Cells and Organs. K.K. Kartha (ed.), CRC Press Inc., Boca Raton, Florida, pp. 115–134.
Kartha, K.K. (1986). Production and indexing of disease free plants. In: Pant Tissue Culture and its Agricultural Applications. L.A. Withers and P.G. Anderson (eds.), Butterworths, London, pp. 219–238.
Kartha, K.K. and Gamborg, O.L. (1975). Elimination of cassava mosaic disease by meristem culture. Phytopathology 65: 826–828.
Kartha, K.K. and Gamborg, O.L. (1978). Meristem culture techniques in the production of disease-free plants and freeze-preservation of germplasm of tropical tuber crops and grain legumes. In: Diseases of Tropical Food Crops. H. Maraite and J.A. Mayer (eds.), Université Catholique, Louvain-la-Neuve, pp. 267–283.
Kartha, K.K., Gamborg, O.L. and Constabel, F. (1974a). Regeneration of pea (Pisum sativum L.) plants from shoot apical meristems. Z. Pflanzenphysiol. 72: 172–176.
Kartha, K.K., Gamborg, O.L. and Constabel, F. (1974b). Regeneration of cassava plants from apical meristems. Plant Sci. Lett. 2: 107–113.
Kartha, K.K., Leung, N.L. and Gamborg, O.L. (1979). Freeze-preservation of pea meristems in liquid nitrogen and subsequent plant regeneration. Plant Sci. Lett. 15: 7–15.
Kartha, K.K., Leung, N.L. and Pahl, K. (1980). Cryopreservation of strawberry meristems and mass propagation of plantlets. J. Am. Soc. Hort. Sci. 105: 481–484.
Kartha, K.K., Mroginski, L.A., Pahl, K. and Leung, N.L. (1981a). Germplasm preservation of coffee (Coffea arabica L.) by in vitro culture of shoot apical meristems. Plant Sci. Lett. 22: 301–307.
Kartha, K.K., Pahl, K., Leung, N.L. and Mroginski, L.A. (1981b). Plant regeneration from meristems, of grain legumes: soybean, cowpea, peanut, chickpea, and bean. Can. J. Bot. 59: 1671–1679.
Klein, R.E. and Livingstone, C.H. (1982). Eradication of potato virus X from potato by ribavirin treatment of cultured potato shoot tips. Am. Potato J. 59: 359–365.
Kondakova, V. and Schuster, G. (1991). Elimination of strawberry mottle virus and strawberry crincle virus from isolated apices of three strawberry varieties by the addition of 2,4-dioxohexahydro-1,3,5-triazine (S-Azadihydrouracil) to the nutrient medium. J. Phytopathology 132: 8486.
Krogstrup, P. and Thomsen, A. (1985). Use of meristem culture in vitro for elimination of raspberry ringspot virus (RRSV) in red current (Ribesn L. cv. Random). In: In vitro Techniques-Propagation and Long Term Storage. A. Schaefer-Menuhr (ed.), Martinus Nijhoff Publishers, Dordrecht, pp. 79–83.
Kudell, A.R. and Buchenauer, H. (1989). Elimination of raspberry dwarf virus from axillary bud cultures of red raspberry cv. Lloyd George by antiviral compounds. J. Phytopathology 124: 332–336.
Kukreja, A.K., Mathur, A.K. and Zaim, M. (1990). Mass production of virus-free patchouli plants, Pogostemon cablin (Balnco) Benth., by in vitro culture. Trop. Agric. 67: 101–104.
Lane, W.D., Looney, N.E. and Mage, F. (1982). A selective tissue culture medium for growth of compact (dwarf) mutants of apple. Theor. Appl. Genet. 61: 219–223.
Lane, W.D. and McDougald, J.M. (1982). Shoot tip culture of apple: comparative response of five cultivars to cytokinin and auxin. Can. J. Plant Sci. 62: 689–694.
Lawson, R.H. (1986). Pathogen detection and elimination. In: Tissue Culture as a Plant Production System for Horticultural Crops. R.H. Zimmerman (ed.), Martinus Nijhoff Publishers, Dordrecht, pp. 97–117.
Lawson, C., Kaniewski,.W., Haley, L., Rozman, R., Newell, C., Sanders, P. and Turner, N.E. (1990). Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Bio/Technology 8: 127–134.
Lee, E.C.M. and de fossard, R.A. (1977). Some factors affecting multiple bud formation of strawberry (Fragaria x ananassa Duch.). Acta. Hort. 78: 187–195.
Limmaset, P. and Cornuet, P. (1949). Recherche de virus de la mosaique du tabac (Marmor Tabaci Holmes) dans les meristemes des plantes infectées. Compt. Rend. 228: 1971–1972.
Litz, R.E. and Conover, R.A. (1981). Effect of sex type, season, and other factors on in vitro establishment and culture of Carica papaya L. explants. J. Am. Soc. Hort. Sci. 106: 792–794.
Litz, R.E. and Jaiswal, V.S. (1991). Micropropagation of tropical and subtropical fruits. In: Micropropagation–Technology and Application. P.C. Debergh and R.H. Zimmerman (eds.), Kluwer Academic Publishers, Dordrecht, pp. 247–264.
Lommel, S.A., McCain, A.H. and Morris, T.J. (1982). Evaluation of indirect enzyme-linked immunosorbent assay for the detection of plant viruses. Phytopathology 72: 1018–1022.
Lozoya-Saldana, H. and Dawson, W.O. (1982). Effect of alternating temperature regimes on reduction or elimination of viruses in plant tissues. Phytopathologyy 72: 1059–1064.
Lozoya-Saldana, H. and Merlin-Lara, O. (1984). Thermotherapy and tissue culture for elimination of potato virus X (PVX) in Mexican potato cultivars resistant to late blight. Am. Potato J. 61: 735–739.
Lozoya-Saldana, H. and Madrigal-Vargas, A. (1985). Kinetin, thermotherapy, and tissue culture to eliminate potato virus (PVX) in potato. Am. Potato J. 62:339–345.
Lundergan, C.A. and Janick, J. (1980). Regulation of apple shoot proliferation and growth in vitro. Hort. Res. 20: 19–24.
Marcotrigiano, M., Swartz, H.J., Gray, S.E., Tokaric, D. and Popenoe, J. (1984). The effect of benzylaminopurine on the in vitro multiplication rate and subsequent field performance of tissue culture propagated strawberry plants. Recent Adv. Strawberry Prod. 3: 23–25.
Martin, R.R. (1987). The use of monoclonal antibodies for virus detection. Can. J. Plant Pathol. 9: 177–181.
McCown, B.H. and Lloyd, G.B. (1983). A survey of response of Rhododendron to in vitro culture. Plant Cell Tissue Org. Cult. 2: 77–85.
Mellor, F.C. and Stace-Smith, R. (1969). Development of excised potato buds in nutrient medium. Can. J. Bot. 47: 1617–1621.
Mellor, F.C. and Stace-Smith, R. (1987). Virus-free potatoes through meristem culture. In: Biotechnology in Agriculture and Forestry, Vol. 3: Potatoes. Y.P.S. Bajaj (ed.), Springer-Verlag, New York, pp. 30–39.
Miller, S.A. and Martin, R.R. (1988). Molecular diagnosis of plant disease. Ann. Rev. Phytopathol. 26: 409–432.
Mohamed, N.A. and Imperial, J.S. (1984). Detection and concentration of coconut cadangcadang viroid in coconut leaf extracts. Phytopathology 74: 165–169.
Monette, P.L. (1983). Virus eradication through in vitro techniques. Int. Plant Propag. Soc. Comb. Proc. 33: 90–100.
Monette, P.L. (1986). Elimination in vitro of two grapevine nepoviruses by an alternating temperature regime. J. Phytopathology 116: 88–91.
Morel, G. (1960). Producing virus-free cymbidium. Am. Orchid Soc. Bull. 29: 495–497.
Morel, G.M. (1972). Morphogenesis of stem apical meristem cultivated in vitro: Application to clonal propagation. Phytomorphology 22,: 265–277.
Morel, G. and Martin, C. (1952). Guerison de dahlias atteints d’une malade à virus. C.R. Acad. Sci., Paris 235: 1324–1325.
Morel, G. and Martin, C. (1955). Guerison de pommes de terre atteintis de malades à virus. C.R. Acad. Agric. Fr. 41:471–474.
Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
Murashige, T. (1974). Plant propagation through tissue culture. Ann. Rev. Plant Physiol. 25: 135–166.
Murashige, T. (1977). Clonal crops through tissue culture. In: Plant Tissue Culture and its Biotechnological Application. W. Barz, E. Reinhard and M.H. Zenk (eds.), Springer-Verlag, New York, pp. 392–403.
Muriithi, L.M., Rangan, T.S. and Waite, B.H. (1982) In vitro propagation of fig through shoot tip culture. Hort. Sc. 17:86–87.
Nehra, N.S. and Singh, K. (1982). Propagation of fruit crops through tissue culture–a review. Haryana J. Hort. Sci. 11: 13–16.
Nehra, N.S., Stushnoff, C. and Kartha, K.K. (1989). Direct shoot regeneration from strawberry leaf disks J Am. Soc. Hort. Sci. 114: 1014–1018.
Nehra, N.S., Stushnoff, C. and Kartha, K.K. (1990a). Regeneration of plants from immature leaf-derived callus of strawberry (Fragaria x ananassa). Plant Sci. 66: 119–126.
Nehra, N.S., Chibbar, R.N., Kartha, K.K., Datla, R.S.S., Crosby, W.L. and Stushnoff, C. (1990b). Genetic transformation of strawberry by Agrobacterium tumefaciens using a leaf disk regeneration system. Plant Cell Rep. 9: 293–298.
Norton, M.E. and Norton, C.R. (1985). In vitro propagation of Ericaceae: a comparison of the activity of the cytokinins N6-benzyladenine and N6-isopentyladenine in shoot proliferation. Sci. Hort. 27:335–340.
Novak, F.J. and Jovova, Z. (1983). Clonal propagation of grapevine through in vitro axillary bud culture. Sci. Hort. 18: 231–240.
Paduch-Cichal, E. and Kryczynski, S. (1987). A low temperature therapy and meristem-tip culture for eliminating four viroids from infected plants. J. Phytopathology 118: 341–346.
Pierik, R.L.M. (1991). Commercial propagation in Western Europe and Israel. In: Micropropagation–Technology and Application. P.C. Debergh and R.H. Zimmerman (eds.), Kluwer Academic Publishers, Dordrecht, pp. 155–166.
Pink, D.A.C., and Walkey, D.G.A. (1984). Rapid propagation of Cucurbita pepo L. by culture of meristem tips. Sci. Hort. 24: 107–114.
Polston, J.E., Dodds, A.J. and Perring, T.M. (1989). Nucleic acid probes for detection and strain discrimination of cucurbita geminiviruses. Phytopathology 79: 1123–1127.
Pua, E-C. and Chong, C. (1984). Requirement for sorbitol (D-glucitol) as carbon source for in vitro propagation of Malus robusta No. 5. Can. J. Bot. 62: 1545–1549.
Reichenbacher, D., Reiss, E., Hunger, H.-D. and Richter, J. (1990). Detection of plant viruses by ELISA using different reagent strips. J. Phytopathology 128: 333–339.
Robinson, D.J., Harrison, B.D., Sequeira, J.C. and Duncan, G.H. (1984). Detection of strains of African cassava mosaic virus by nucleic acid hybridization and some effects of temperature on their multiplication. Ann. Appl. Biol. 105: 483–493.
Roca, W.M., Chavez, R., Martin, M.L., Arias, D.I., Mafla, G. and Reyes, R. (1989). In vitro methods of germ-plasm conservation. Genome 31:813–817.
Roy, B.P., AbouHaidar, M.G., Sit, T.L. and Alexander, A. (1988). Construction and use of cloned cDNA biotin and 32P-labeled probes for the detection of papaya mosaic potex virus RNA in plants. Phytopathology 78: 1425–1429.
Rubluo, A. and Kartha, K.K. (1985). In vitro culture of shoot apical meristems of various Phaseolus species and cultivars. J. Plant Physiol. 119:425–433.
Sakai, A. (1985). Cryopreservation of shoot tips of fruit trees and herbaceous plants. In: Cryopreservation of Plant Cells ànd Organs. K.K. Kartha (ed.), CRC Press, Boca Raton, Florida, pp. 135–158.
Samartin, A. (1989). A comparative study of effects of nutrient media and culture conditions on shoot multiplication of in vitro cultures of Camellia japonica explants. J. Hort. Sci. 64: 73–79.
Samyn, G.L., Debergh, P.C. and Vermaerke, D. (1984). Field performance and phenotypic stability of virus-free tissue cultured Begonia x tuberhybrida `Multiflora’. Sci. Hort. 24: 185–191.
Schaeffer, W.G., Damiano, C., Scott, D.H., McGrew, J.R., Krul, W.R. and Zimmerman, R.H. (1980). Transcription of panel discussion on the genetic stability of tissue culture propagated plants. In: Proceedings of the Conference on Nursery Production of Fruit Plants Through Tissue Culture-Applications and Feasibility. USDA-SEA, Agric. Res. Results, ARE-NE-11, pp. 64–79.
Schrammeijer, B., Sijmons, P.C., van den Elzen, P.J.M. and Hoekema, A. (1990). Meristem transformation of sunflower via Agrobacterium. Plant Cell Rep. 9: 55–60.
Scott, D.H. and Zanzi, C. (1980). Rapid propagation of strawberry from meristems. In: The Strawberry-Cultivars to Marketing. N.F. Childers (ed.), Horticultural Publications, Gainesville, Florida, pp. 213–221.
Seckinger, G.R. (1991). Micropropagation of vegetable crop species. In: Micropropagation–Technology and Application. P.C. Debergh and R.H. Zimmerman (eds.), Kluwer Academic Publishers, Dordrecht, pp. 265–284.
Sela, I., Reichman, M. and Weissbach, A. (1984). Comparison of dot molecular hybridization and enzyme-linked immunosorbent assay for detecting tobacco mosaic virus in plant tissues and protoplasts. Phytopathology 74: 385–389.
Simpkins, I., Walkey, D.G.A. and Neely, H.A. (1981). Chemical suppression of virus in cultured plant tissues. Ann. Appl. Biol. 99: 161–169.
Simpson, D.W. and Bell, J.A. (1989). The response of different genotypes of Fragaria x ananassa and their seedling progenies to in vitro micropropagation and the effect of varying the concentrations of 6-benzylaminopurine in the proliferation medium. Plant Cell Tissue Org. Cult. 17: 225–234.
Singha, S. (1982). In vitro propagation of crabapple cultivars. Hort. Sc. 17:191–192.
Sommer, H.E. (1982). Organogenesis in angiospermous trees. Application to vegetative propagation. Bulletin de la Societé Botanique de la France 72: 128–130.
Stace-Smith, R. and Mellor, F.C. (1968). Eradication of potato virus X and S by thermotherapy and axillary bud culture. Phytopathology 58: 199–203.
Stone, O.M. (1963) Factors affecting the growth of carnation plants from shoot apices. Ann. Appl. Biol. 52: 199–209.
Stone, O.M. (1982). The elimination of four viruses from Ullucus tuberosus by meristem tip culture and chemotherapy. Ann. Appl. Biol. 101: 79–83.
Styer, D.J. and Chin, C.K. (1983). Meristem and shoot tip culture for propagation, pathogen elimination and germplasm preservation. Hort. Rev. 5: 221–277.
Swartz, H.J., Galletta, G.J. and Zimmerman, R.H. (1981). Field performance and phenotypic stability of tissue-culture-propagated strawberries. J. Am. Soc. Hort. Sci. 106: 667–673.
Swartz, H.J., Galletta, G.J. and Zimmerman, R.H. (1983). Field performance and phenotypic stability of tissue-culture-propagated thornless blackberries. J. Am. Soc. Hort. Sci. 108: 285–290.
Tisserat, B. (1984). Propagation of date palms by shoot tip cultures. Hort. Sc. 19: 230–231.
Theiler-Hedtrich, R. and Baumann, G. (1989). Elimination of apple mosaic virus and raspberry bushy dwarf virus from infected red raspberry (Rubus idaeus L.) by tissue culture. J. Phytopa-thology 127: 193–199.
Toussaint, A., Dekegel, D. and Vanheule, G. (1984). Distribution of Odontoglossum ringspot virus in apical meristems of infected Cymbidium cultivars. Physiological Plant Pathology 25: 297–305.
Ulian, E.C., Smith, R.H., Gould, J.H. and McKnight, T.D. (1988). Transformation of plants via the shoot apex. In vitro Cell. Dev. Biol. 24,: 951–954.
Vajrabhaya, T. (1977). Variation in clonal propagation. In: Orchid Biology: Review and Per- spectives. J. Arditti (ed.), Columbia University Press, New York, pp. 179–201.
Van den Heuvel, J.F.J.M. and Peters, D. (1989). Improved detection of potato leafroll virus in plant material and in aphids. Phytopathology 79: 963–967.
Van Regenmortel, M.H.V. (1982). Serology and Immunochemistry of Plant Viruses. Academic Press, New York.
Varveri, C., Candresse, T., Cugusi, M., Ravelonandro, M. and Dunez, J. (1988). Use of 32P-labeled transcribed RNA probe for dot hybridization detection of plum pox virus. Phytopathology 78: 1280–1283.
Vasil, I.K. (1991). Cell Culture and Somatic Cell Genetics of Plants, Vol. 8. Scale-up and Automation in Plant Propagation. Academic Press, San Diego.
Vasil, I.K. and Vasil, V. (1980). Clonal propagation. In: Perspectives in Plant Cell and Tissue Culture. I.K. Vasil (ed.), Int. Rev. Cytol., Supplement 11A, Academic Press, New York, pp. 145–174.
Vieitez, A.M., Carmen San-Jose, M. and Vieitez, E. (1985). In vitro plantlet regeneration from juvenile and mature Ouercus robur L. J. Hort. Sci. 60:99–106.
Vieitez, A.M. and Vieitez, M.L. (1983). Castanea sativa plantlets proliferated from axillary buds cultivated in vitro. Sci. Hort. 18: 343–351.
Vine, S.J. and Jones, O.P. (1969). The culture of shoot tips of hop (Humulus lupulus) to eliminate viruses. J. Hort. Sci. 44: 281–284.
Walkey D.G.A (1980) Production of virus-free plant by tissue culture. In: Tissue Culture Methods for Plant Pathologist. D.S. Ingram and J.P. Helgeson (eds.), Blackwell Scientific Publications, Oxford, pp. 109–117.
Wakley, D.G.A., Webb, M.J.W., Bolland, C.J. and Miller, A. (1987). Production of virus-free garlic (Allium sativum L.) and shallot (A. ascalonicum L.) by meristem-tip culture. J. Hort. Sci. 62: 211–220.
Wambugu, F.M., Secor, G.A. and Gudmestad, N.C. (1985). Eradication of potato virus Y and S from potato by chemotherapy of cultured axillary bud tips. Am. Potato J. 62: 667–672.
Wanas, W.H., Callow, J.C. and Withers, L.A. (1986). Growth limitation for the conservation of pear genotypes. In: Plant Tissue Culture and its Agricultural Applications. L.A. Withers. and P.G. Alderson (eds.), Butterworths, London, pp. 285–290.
Watkins, C.A., McNicol, R.J., Young, K. and Jones, A.T. (1990). The effect of heat treatment and meristem tip culture on June yellows in strawberry. Ann. Appl. Biol. 116: 489–492.
Welander, M. (1985). In vitro culture of raspberry (Rubus ideaus) for mass propagation. Hort. Sci. 60:493–500.
White, P.R. (1943). A Handbook of Plant Tissue Culture. Jaques Cattell Press, L.ncaster. Withers, L.A., Wheelans, S.K. and Williams, J.T. (1990). In vitro conservation of crop germ-plasm and IBPGR databases. Euphytica 45: 9–22.
Wochok, R.J. and Sluis, C.J. (1980). In vitro propagation and establishment of wax current (Ribes inebrians) J. Hort. Sci. 55:355–357.
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Nehra, N.S., Kartha, K.K. (1994). Meristem and Shoot Tip Culture: Requirements and Applications. In: Vasil, I.K., Thorpe, T.A. (eds) Plant Cell and Tissue Culture. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2681-8_3
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