Abstract
The genus Hibiscus (Malvaceae) consists of about 200 species, distributed in tropical and subtropical zones, many of which are cultivated as ornamentals. Roselle (Hibiscus sabdariffa L.) is an annual shrub 1.5–2.5 m in height (Fig. 1). The calyces contain red pigments and are used for making jelly, jams, beverages, and food colorants in a number of tropical countries (Clydesdale et al. 1979). Roselle has also been used as a source of plant fiber called roselle hemp (Corner and Watanabe 1969).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Alfermann W, Reinhard E (1971) Isolation of anthocyanin containing and anthocyanin-free callus tissues of Daucus carota: effect of auxin on the anthocyanin formation. Experientia 27:353–354
Beiderheck R, Knoop B (1987) Two-phase culturc. In: Constabel F. Vasil IK (eds) Cell culturc and somatic cell genetics of plants. vol 4 Cell culture in phytochcmistry, Academic Press, San Diego, pp 255–266
Clydesdale FM, Main JH, Francis FJ (1979) Roselle (Hibiscus sabdariffa L.) anthocyanin as food colorants for beverages and gelatin desserts. J Food Protect 42:204–207
Corner EJH, Watanahe K (1969) Hibiscus sabdariffa L. In: Illustrated guides to tropical plants. Hirokawa. Tokyo. 471 pp
Du DT, Francis FJ (1973) Anthocyanins of roselle (Hibiscus sabdariffa L.). J Food. Sci 38:810–812
Fujita Y, Tabata M (1987) Secondary metabolites from plant cells — pharamaceutical applications and progress in commercial production. In: Green CE, Somers DA, Hackett WP, Biesboer DD (eds) Plant biology vol. 3 Plant tissue and cell culturc. Alan R. Liss, New York, pp 169–185
Gregor HD (1974) Effect of gibberellic acid (GA,) on the PAL activity and the synthesis of phenyl-propanoids in cell cultures of Haplopappus gracilis. Protoplasma 80:273–277
Hiraoka N, Kodama T, Tomita Y (19x6) Selection of Bupleurum falcatum callus line producing anthocyanins in darkness. J Nat Prod 49:470–474
Hong V, Wrolstad RE (1990) Use of HPLC separation/photodiode array detection for characterization of anthocyanins. J Agric Food Chem 38:708–715
Jiang N, Yan Y, Tang B (1990) Identification of red pigments of roselle. Shipin Yu Fajiao Gongye No. 3:18–23
Jonadet M, Bastide J, Rastide P, Boyer B, Carnet AP (1990) Enzymc-inhibiting activities in vitro and angioprotectivc activities in vivo of roselle (Hibiscus sabdariffa L.) extracts. J Pharm Belg 45:120–124
Khafaga ER. Koch H (1980) Stage of maturity and quality of karkadeh (Hibiscus sabdariffa L. var. sabdariffa) II Anlhocyanins. Angew Bot 54:295–300
Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacc? tissue cultures. Physiol Plant 18:100–127
Miyasaka H, Nasu M, Yamamoto T, Endo Y, Yoneda K (1986) Regulation of ferruginol and cryptotanshinone biosynthesis in cell suspension cultures of Salvia miltiorrhiza. Phytochemistry 25:637–640
Mizukami H (1991) Production of pigments by plant tissue culture — roselle. Fragrance J 4:54–57
Mizukami H, Tomita K, Ohashi H Hiraoka N (1988) Anthocyanin production in callus cultures of roselle (Hibiscus sabdariffa L.). Plant Cell Rep 7:553–556
Mizukami H, Tomita K, Ohashi H (1989) Anthocyanin accumulation and changes in activities of phenylalanine ammonia-lyase and chalcone synthase in rode (Hibiscus sabdariffa L.) callus cultures. Plant Cell Rep 8:467–470
Mizukami H, Nakamura M, Tomita K, Higuchi K, Ohashi H (1991) Effects of macronutrients on anthocyanin production in roselle (Hibiscus sabdariffa L.) cell cultures. Plant Tissue Cult Lett 8:14–20
Ozeki Y, Komamine A (1986) Effects of growth regulators on the induction of anthocyanin synthesis in carrot suspension cultures. Plant Cell Physiol 27:1361–1368
Pouget MP, Lejeune B, Pourrat A (1988) Study on the stability of Hibiscus dye in gels. S. T. P. Pharma 4:103–105
Pouget MP, Vennet B, Lejeune B, Pourrat A (1990a) Identification of anthocyanins of Hibiscus sabdariffa L. Lebensm Wiss Technol 23:101–102
Pouget MP, Vennet B, Lejeune B, Pourrat A (1990b) Extraction, analysis and study of the stability of Hibiscus anthocyanins. Lebensm Wiss Technol 23:103–105
Sakuta M, Komamine A (1987) Cell growth and accumulation of secondary metabolites. In: Constabel F, Vasil IK (eds) Cell culture and somatic cell genetics of plants vol 4. Cell culture in phytochemistry, Academic Press, San Diego, pp 97–114
Seitz HU, Hinderer W (1988) Anthocyanins. In: Constabel F, Vasil IK (eds) Cell culture and somatic cell genetics of plants, vol 5. Phytochemicals in plant cell cultures, Academic Press, San Diego, pp 49–76
Smith SL, Slywka GW, Kreuger RJ (1981) Anthocyanins of Strobilanthes dyeriana and their production in callus cultures. J Nat Prod 44:609–610
Yamakawa T, Ishida K, Kato S, Kodama T, Minoda Y (1983a) Formation and identification of anthocyanins in cultured cells of Vitis sp. Agric Biol Chem 47:997–1001
Yamakawa T, Kato S, Ishida K, Kodama T, Minoda Y (1983b) Production of anthocyanins by Vitis cells in suspension culture. Agric Biol Chem 47:2185–2191
Yamamoto R, Oshima Y (1932) Red coloring matter of Hibiscus sabdariffa L. Sci Pap Inst Phys Chem Res Tokyo 19:134–141
Yamamoto R, Oshima Y (1936) Coloring matter of Hibiscus sabdariffa L. (Hibiscin) II. Sci Pap Inst Phys Chem Res Tokyo 30:258–262
Zeng H, Dong Y, Yang J (1984) Preparation and application of red pigments from Hibiscus sabdariffa L. I Selection and optimization of the most optimum extraction conditions. Shipin Yu Fajiao Gongye 5:27–32
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Mizukami, H. (1993). Hibiscus sabdariffa L. (Roselle): In Vitro Culture and the Production of Anthocyanins. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants V. Biotechnology in Agriculture and Forestry, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58062-8_16
Download citation
DOI: https://doi.org/10.1007/978-3-642-58062-8_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-63459-8
Online ISBN: 978-3-642-58062-8
eBook Packages: Springer Book Archive