Abstract
Gametogenesis of Porphyra yezoensis thalli is induced by ageing as well as by changing water temperature and photoperiod. Under laboratory conditions, thalli cultivated at 10 °C with a 10:14 h (light: dark) cycle develop vegetatively to adult form without gametogenesis. On the other hand, sexual reproduction, which involves differentiation of vegetative cells and subsequent gametogenesis, is induced by culturing at 15 °C with a 16: 8 h (light: dark) cycle. We have constructed subtracted cDNA libraries enriched for differentially expressed transcripts in vegetative and reproductive thalli, and randomly selected 1,152 cDNAs from each subtracted library. Results of the dot blot analyses used for identification of differentially expressed cDNAs indicated that mRNA levels of 176 and 138 cDNAs tended to increase in the vegetative and reproductive thalli, respectively. BLAST analysis of nucleotide and deduced amino acid sequences showed that the cDNAs represented 63 and 59 unique clones for the vegetative and reproductive cDNA enriched subtracted libraries, respectively. Interestingly, some of the cDNAs isolated from the reproductive subtracted library were homologous to genes encoding protein kinases, GTP-binding protein, and heat shock proteins involved in signal transduction and the molecular chaperon system.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
References
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J. Mol. Biol. 215: 403–410.
Altschul SF, Madden TL, Schäffer AA, Zhang J, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402.
Asamizu E, Nakajima M, Kitade Y, Saga N, Nakamura Y, Tabata S (2003) Comparison of RNA expression profiles between the two generations of Porphyra yezoensis (Rhodophyta), based on expressed sequence tag frequency analysis. J. Phycol. 39: 923–930.
Balch WE (1990) Small GTP-binding proteins in vesicular transport. Trends in Biochem. Sci. 15: 473–477.
Bourne HR, Sanders DA, McCormick F (1990) The GTPase superfamily: A conserved switch for diverse cell functions. Nature 348: 125–132.
Cole KM, Conway E (1975) Phenetic implications of structural features of the perennating phase in the life history of Porphyra and Bangia (Bangiophyceae, Rhodophyta). Phycologia 16: 205–216.
Diatchenko L, Lau YFC, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD (1996) Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. PNAS 93: 6025–6030.
Dietmaier W, Fabry S, Huber H, Schmitt R (1995) Analysis of a family of ypt genes and their products from Chlamydomonas reinhardtii. Gene 158: 41–50.
Hall, A (1990) The cellular functions of small GTP-binding proteins. Science 249: 635–640.
Hardie GD, Carling D, Carlson M (1998) The AMP-activated/SNF1 protein kinase subfamily: Metabolic sensors of the eukaryotic cell? Annu. Rev. Biochem. 67: 821–855.
Hong YK, Sohn CH, Polne-Fuller M, Gibor A (1995) Differential display of tissue-specific messenger RNAs in Porphyra perforata (Rhodophyta) thallus. J. Phycol. 31: 640–643.
Inaba T, Nagano Y, Sakakibara T, Sasaki Y (1999) Identification of a cis-regulatory element involved in phytochrome down-regulated expression of the pea small GTPase gene pra2. Plant Physiol. 120: 491–499.
Iwasaki, H (1979) 2.2.E(IV) Porphyra tenera (Rhodophyta). In Nishizawa, K., Chihara, M. (eds), Methods in Phycological Studies, Kyoritsu Shuppan Co., Ltd., Tokyo, pp. 118–126.
Kapraun DF, Hinson TK, Lemus AJ (1991) Karyology and cytophotometric estimation of inter-and intraspecific nuclear DNA variation in four species of Porphyra (Rhodophyta). Phycologia 30: 458–466.
Kuwano K, Aruga Y, Saga N (1996) Cryopreservation of clonal gametophytic thalli of Porphyra (Rhodophyta). Plant Sci. 116: 117–124.
Lee YK, Kim SH, Hong CB, Chah OK, Kim GH, Lee IK (1998) Heat shock protein 90 may be involved in differentiation of the female gametophytes in Griffithsia japonica (Ceramiales, Rhodophyta). J. Phycol. 34: 1017–1023.
Le Gall Y, Brown S, Marie D, Mejjad M, Kloareg B (1993) Quantification of nuclear DNA and GC content in marine microalgae by flow cytometry of isolated nuclei. Protoplasma 173: 123–132.
Liu QY, Meer JP, Reith ME (1994a) Isolation and characterization of phase-specific complementary DNAs from sporophytes and gametophytes of Porphyra purpurea (Rhodophyta) using subtracted complementary DNA libraries. J. Phycol. 30:513–520.
Liu QY, Reith ME (1994b) Isolation of a gametophyte-specific cDNA encoding a lipoxygenase from the red alga Porphyra purpurea. Mol. Mar. Biol. Biotechnol. 3: 206–209.
Liu QY, Baldauf SL, Reith ME (1996a) Elongation factor 1α genes of the red alga Porphyra purpurea include a novel, developmentally specialized variant. Plant Mol. Biol. 31: 77–85.
Liu QY, Ross N, Lanthier P, Reith M (1996b)A gametophyte cell wall protein of the red alga Porphyra purpurea (Rhodophyta) contains four apparent polysaccharide-binding domains. J. Phycol. 32: 995–1003.
Liu QY, Reith M (1996c) A sporophyte cell wall protein of the red alga Porphyra purpurea (Rhodophyta) is a novel member of the chymotrypsin family of serine proteases. J. Phycol. 32: 1003–1009.
Marrs KAE, Casey ES, Capitant SA, Bouchard RA, Dietrich PS, Mettler IK, Sinibaldi RM (1993) Characterization of two maize hsp90 heat shock protein genes and expression during heat shock, embryogenesis, and pollen development. Dev. Genet. 14: 27–41.
Mukai LS, Craigie JS, Brown RG (1981) Chemical composition and structure of the cell walls of the conchocelis and thallus phases of Porphyra tenera (Rhodophyceae). J. Phycol. 17: 192–198.
Nikaido I, Asazumi E, Nakajima M, Nakamura Y, Saga N, Tabata S (2000) Generation of 10,154 expressed sequence tags from a leafy gametophyte of a marine red alga, Porphyra yezoensis. DNA Res. 7: 223–227.
Pratt WB, Krishna P, Olsen LJ (2001) Hsp90-binding immunophilins in plants: the protein movers. Trends Plant Sci. 6: 54–58.
Provasoli, L (1968) Media and prospects for the culltivation of marine algae. In Watanabe, A., Hattori, A. (eds), Culture and Collection of Algae: Proceedings of US-Japan Conference in Hakone, Japanese Society of Plant Physiologists, Tokyo, pp. 63–75.
Schaeffer HJ, Weber MJ (1999) Mitogen-activated protein kinases: Specific messages from ubiquitous messengers. Mol. Cell. Biol. 19: 2435–2444.
Waaland JR, Stiller JW, Cheney DP (2004) Macroalgal candidates for genomics. J. Phycol. 40: 26–33.
Yabe N, Takahashi T, Komeda Y (1994) Analysis of tissue-specific expression of Arabidopsis thaliana hsp90-family gene HSP81. Plant Cell Physiol. 35: 1207–1219.
Yoshida K, Nagano Y, Murai N, Sasaki Y (1993) Phytochrome-regulated expression of the genes encoding the small GTP-binding proteins in peas. PNAS 90: 6636–6640.
Zemke-White WL, Ohno M (1999) World seaweed utilization: An end-of-century summary. J. Appl. Phycol. 11: 369–376.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Springer
About this paper
Cite this paper
Kakinuma, M., Kaneko, I., Coury, D.A., Suzuki, T., Amano, H. (2006). Isolation and identification of gametogenesis-related genes in Porphyra yezoensis (Rhodophyta) using subtracted cDNA libraries. In: Anderson, R., Brodie, J., Onsøyen, E., Critchley, A.T. (eds) Eighteenth International Seaweed Symposium. Developments in Applied Phycology, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5670-3_33
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5670-3_33
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5669-7
Online ISBN: 978-1-4020-5670-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)