13.3 Summary and Conclusion
An in vitro preparation of the chicken retina was established on multi-electrode arrays. The so-called retinasensor allows the multi-site recording of micro-ERGs for hours and has been shown to be sensitive enough to assess drug effects on retinal function. The advantage of the system is that the retina can be isolated together with the retinal pigment epithelium as a whole by merely cutting the optic nerve. In contrast with other brain preparations, slicing of the tissue can be avoided and thus no damaged cells are present at the electrode-tissue interface that would interfere with the stimulation of healthy cell layers. A further advantage of the retinasensor is the recording of both micro-ERGs and ganglion cell activity. Because retinal ganglion cells do not contribute much to the ERG, additional information on the status of ganglion cells can be provided with the retinasensor as compared to ERG recordings in vivo. Taken together, the retinasensor offers a new assay system to reliably assess retinal function and dysfunction in vitro.
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References
Bolz, H., von Brederlow, B., Ramirez, A., Bryda, E.C., Kutsche, K., Nothwang, H.G., Seeliger, M., C.-Salcedó Cabrera, M., Caballeró Vila, M., Pelaez Molina, O., Gal, A., and Kubisch, C. (2001). Mutations in cadherin-23 (otocadherin), a novel member of the cadherin gene family, cause Usher syn-drome type 1D. Nat. Gene. 27: 108–112.
Brown, K.T., and Wiesel, T.N. (1961a). Analysis of the intraretinal electroretinogram in the intact cat eye. J. Physiol. (London) 158: 229–256.
Brown, K.T., and Wiesel, T.N. (1961b). Localization of origins of electroretinogram components by intraretinal recording in the intact cat eye. J. Physiol. (London) 158: 257–280.
Einthoven, W., and Jolly, W.A. (1908). The form and magnitude of the electrical response of the eye to stimulation by light at various intensities. Quaterly J. Exp. Physiol. 1: 373–416.
Granit, R. (1933). The components of the retinal action potential in mammals and their relation to the discharge in the optic nerve. J. Physiol. (London) 77: 207–239.
Gurevich, L., Slaughter, M. M. (1993). Comparison of the waveforms of the ON bipolar neuron and the b-wave of the electroretinogram. Vis. Res. 33: 2431–2435.
Hood, D.C. and Birch, D.G. (1995). Phototransduction in human cones measured using the a-wave of the ERG. Vis. Res. 35: 2801–2810.
Jamison, J.A., Bush, R.A., Lei, B., and Sieving, P. (2001). Characterization of the rod photoresponse isolated from the dark-adapted primate ERG. Vis. Neurosci. 18: 445–455.
Kapousta-Bruneau, N.V. (2000). Opposite effects of GABAA and GABAC receptor antagonists on the b-wave of ERG recorded from the isolated rat retina. Vis. Res. 40: 1653–1665.
Kline, R.P., Ripps, H., and Dowling, J.E. (1985). Light-induced potassium fluxes in the skate retina. Neuroscience 14: 225–235.
Kolb, H. (1991). The neural organization of the human retina. In: Heckenlively, J.R. and Arden, G.B., eds., Principles and Practices of Clinical Electrophysiology of Vision. Mosby Year Book, St. Louis, pp. 25–52.
Kueng-Hitz, N., Grimm, C., Lansel, N., Hafezi, F., He, L., Fox, D.A., Reme, C.E., Niemeyer, G., and Wenzel, A. (2001). The retina of c-fos-/-mice: electrophysiologic, morphologic and biochemical aspects. Invest. Ophthalmol. Vis. Sci. 41(3): 909–916.
Lei, B. and Perlman, I. (1999). The contribution of voltage-and time-dependent poassium conductances to the electroretinogram in rabbits. Vis. Neurosci. 16: 743–754.
Marmor, M.F. and Zrenner, E. (1998). Standard for clinical electroretinography. Doc. Ophthalmol. 97: 143–156.
Miller, R.F., and Dowling, J.E. (1970). Intracellular responses of the Müller (glial) cells of the mudpuppy retina: their relation to the b-wave of the electroretinogram. J. Neurophysiol. 33: 323–341.
Mizota, A. and Adachi-Usami, E. (2002). Effect of body temperature on electroretinogram of mice. Invest. Ophthalmol. Vis. Sci. 43(12): 3754–3757.
Nawy, S. and Jahr, C.E. (1990). Suppression by glutamate of cGMP-activated conductance in retinal bipolar cells. Nature 346: 269–271.
Newman, E.A. (1985). Current source-density analysis of the b-wave of frog retina. J. Neurophysiol. 43: 1355–1366.
Noell, W.K. (1954). The origin of the electroretinogram. Am. J. Ophthalmol. 30: 78–90.
Ookawa, T. (1971). Effects of acute hypothermia on the chick ERG. Experientia 27(4): 405–407.
Perlman, I. (2003). The electroretinogram. http://webvision.med.utah.edu, Part XI.
Polyak, S.L. (1941). The Retina. University of Chicago Press, Chicago.
Rodieck, R.W. (1973). The Vertebrate Retina: Principles of Structure and Function. W.H. Freeman, San Francisco.
Schmid, S., and Guenther, E. (1998). Alterations in channel density and kinetic properties of the sodium current in retinal ganglion cells of the rat during in vivo differentiation. Neuroscience 85(1):249–258.
Schwahn, H.N., Kaymak, H., and Schaeffel, F. (2000). Effects of atropine on refractive development, dopamine release, and slow retinal potentials in the chick. Vis. Neurosci. 17(2): 165–176.
Sieving, P.A., Murayama, K., and Naarendorp, F. (1994). Push-pull model of the primate photopic electroretinogram: A role for hyperpolarizing neurons in shaping the b-wave. Vis. Neurosci. 11: 519–532.
Sillman, A.J., Ito, H., and Tomita, T. (1969a). Studies on the mass receptor potential of the isolated frog retina. I. General properties of the response. Vis. Res. 9: 1435–1442.
Sillman, A.J., Ito, H., and Tomita, T. (1969b). Studies on the mass receptor potential of the isolated frog retina. II. On the basis of the ionic mechanism. Vis. Res. 9: 1443–1451.
Slaughter, M.M. and Miller, R.F. (1981). 2-amino-4-phosphobutyric acid: A new pharmacological tool for retina research. Science 211: 182–184.
Steinberg, R.H., Schmidt, R., and Brown, K.T. (1970). Intracellular responses to light from cat pigment epithelium: Origin of the electroretinogram c-wave. Nature 227: 728–730.
Tomita, T. (1950). Studies on the intraretinal action potential. I. Relation between the localization of micropipette in the retina and the shape of the intraretinal action potential. Jap. J. Physiol. 1: 110–117.
Wioland, N. and Rudolf, G. (1991). Light and dark induced variations of the c-wave voltage of the chicken eye after treatment with sodium aspartate. Vis. Res. 31(4): 643–648.
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Guenther, E., Herrmann, T., Stett, A. (2006). The Retinasensor: An In Vitro Tool to Study Drug Effects on Retinal Signaling. In: Taketani, M., Baudry, M. (eds) Advances in Network Electrophysiology. Springer, Boston, MA . https://doi.org/10.1007/0-387-25858-2_13
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