Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1119)
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About this book
With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis.
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Keywords
Table of contents (18 protocols)
Editors and Affiliations
Bibliographic Information
Book Title: Human Cytomegaloviruses
Book Subtitle: Methods and Protocols
Editors: Andrew D. Yurochko, William E. Miller
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-788-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer New York 2014
Hardcover ISBN: 978-1-62703-787-7Published: 18 March 2014
Softcover ISBN: 978-1-4939-6273-0Published: 03 September 2016
eBook ISBN: 978-1-62703-788-4Published: 17 March 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 368
Number of Illustrations: 18 b/w illustrations, 22 illustrations in colour
Topics: Infectious Diseases, Virology