Overview
- Editors:
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Pedro R. Cutillas
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Bart’s and the London School of Medicine, Institute of Cancer, Queen Mary, Universtiy of London, London, United Kingdom
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John F. Timms
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, EGA Institute for Women's Health, University College London, London, United Kingdom
- Examines current LC-MS technologies, instrumentation, applications, and analysis solutions used in quantitative proteomics
- Details protocols for isobaric tagging, in vivo stable isotopic labelling, label-free quantification, and multiple reaction monitoring
- Covers the analysis of protein post-translational modifications, protein complexes, organelles, and kinase activities
- Includes supplementary material: sn.pub/extras
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About this book
With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.
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Table of contents (21 protocols)
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Overviews of Techniques and Approaches in LC-MS-Based Proteomics
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- Pedro R. Cutillas, John F. Timms
Pages 3-17
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- John F. Timms, Pedro R. Cutillas
Pages 19-45
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- Hannah Johnson, Claire E. Eyers
Pages 93-108
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Structural Analysis of Post-Translational Modifications
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Front Matter
Pages 109-109
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- Maria P. Alcolea, Pedro R. Cutillas
Pages 111-126
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- Bertran Gerrits, Bernd Bodenmiller
Pages 127-136
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- Theodora Tryfona, Elaine Stephens
Pages 137-151
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Techniques for Quantitative LC-MS
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Front Matter
Pages 153-153
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- Reiko Kiyonami, Bruno Domon
Pages 155-166
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- Andrew James, Claus Jorgensen
Pages 167-185
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- Kristoffer T.G. Rigbolt, Blagoy Blagoev
Pages 187-204
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- Yishai Levin, Sabine Bahn
Pages 217-231
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Analysis of Protein Complexes and Organelles
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Front Matter
Pages 233-233
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- Andrew J. Thompson, Ritchie Williamson
Pages 235-253
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- Joern Dengjel, Lis Jakobsen, Jens S. Andersen
Pages 255-265
Editors and Affiliations
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Bart’s and the London School of Medicine, Institute of Cancer, Queen Mary, Universtiy of London, London, United Kingdom
Pedro R. Cutillas
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, EGA Institute for Women's Health, University College London, London, United Kingdom
John F. Timms