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Approaches and Applications of Quantitative LC-MS for Proteomics and Activitomics

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LC-MS/MS in Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 658))

Abstract

LC-MS is a powerful technique in biomolecular research. In addition to its uses as a tool for protein and peptide quantization, LC-MS can also be used to quantify the activity of signalling and metabolic pathways in a multiplex and comprehensive manner, i.e. as an ‘activitomic’ tool. Taking cancer research as an illustrative example of application, this review discusses the concepts of biochemical pathway analysis using LC-MS-based proteomic and activitomic techniques.

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Authors and Affiliations

  1. Analytical Signalling Group, Centre for Cell Signalling, Institute of Cancer, Bart’s and the London School of Medicine, Queen Mary University of London, London, UK

    Pedro R. Cutillas

  2. Cancer Proteomics Laboratory, EGA Institute for Women’s Health, University College London, London, UK

    John F. Timms

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  1. Pedro R. Cutillas
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Editors and Affiliations

  1. Bart’s and the London School of Medicine, Institute of Cancer, Queen Mary, Universtiy of London, Charterhouse Square, London, EC1M 6BQ, United Kingdom

    Pedro R. Cutillas

  2. , EGA Institute for Women's Health, University College London, Gower Street, London, WC1E 6BT, United Kingdom

    John F. Timms

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Cutillas, P.R., Timms, J.F. (2010). Approaches and Applications of Quantitative LC-MS for Proteomics and Activitomics. In: Cutillas, P., Timms, J. (eds) LC-MS/MS in Proteomics. Methods in Molecular Biology, vol 658. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-780-8_1

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  • DOI: https://doi.org/10.1007/978-1-60761-780-8_1

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