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Auditory and Vestibular Research

Methods and Protocols

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  • © 2016
  • Latest edition

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Overview

Editors:
  1. Bernd Sokolowski

    1. College of Medicine, University of South Florida, Tampa, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1427)

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About this book

This second edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methods and Protocols, Second Edition guides readers through protocols on cell culture, tissue engineering, nanotechnology, high-throughput screening, and physiology.  Chapters on physiology cover techniques that include optical coherence tomography, patch clamping, and photostimulation of caged neurotransmitters.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Auditory and Vestibular Research: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xvi
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  2. RNA Delivery and Extraction Protocols

    1. Front Matter

      Pages 1-1
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    2. Helios® Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent Updates

      • Inna A. Belyantseva
      Pages 3-26
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    3. Tol2-Mediated Delivery of miRNAs to the Chicken Otocyst Using Plasmid Electroporation

      • Michelle L. Stoller, Donna M. Fekete
      Pages 27-42
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    4. A Rapid, Cost-Effective Method to Prepare Recombinant Adeno-Associated Virus for Efficient Gene Transfer to the Developing Mouse Inner Ear

      • Michelle M. Gomes, Lingyan Wang, Han Jiang, Christoph A. Kahl, John V. Brigande
      Pages 43-57
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    5. Generation of Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannoma and Meningioma

      • Sarah S. Burns, Long-Sheng Chang
      Pages 59-72
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    6. RNA Extraction from Xenopus Auditory and Vestibular Organs for Molecular Cloning and Expression Profiling with RNA-Seq and Microarrays

      • Casilda Trujillo-Provencio, TuShun R. Powers, David R. Sultemeier, Daniel Ramirez-Gordillo, Elba E. Serrano
      Pages 73-92
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  3. Protein Protocols

    1. Front Matter

      Pages 93-93
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    2. Yeast Two-Hybrid Screening to Test for Protein–Protein Interactions in the Auditory System

      • Iman Moeini-Naghani, Dhasakumar S. Navaratnam
      Pages 95-107
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    3. Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research

      • Douglas E. Vetter, Johnvesly Basappa
      Pages 109-133
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    4. Protein Quantitation of the Developing Cochlea Using Mass Spectrometry

      • Lancia N. F. Darville, Bernd H. A. Sokolowski
      Pages 135-148
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    5. Ultrastructural Identification and Colocalization of Interacting Proteins in the Murine Cochlea by Post-Embedding Immunogold Transmission Electron Microscopy

      • Margaret C. Harvey, Bernd H. A. Sokolowski
      Pages 149-164
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    6. Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins

      • Dennis G. Drescher, Selvakumar Dakshnamurthy, Marian J. Drescher, Neeliyath A. Ramakrishnan
      Pages 165-187
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    7. The Single-Molecule Approach to Membrane Protein Stoichiometry

      • Michael G. Nichols, Richard Hallworth
      Pages 189-199
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  4. Imaging Protocols

    1. Front Matter

      Pages 201-201
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    2. Visualization of Live Cochlear Stereocilia at a Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy

      • A. Catalina Vélez-Ortega, Gregory I. Frolenkov
      Pages 203-221
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    3. Design and Construction of a Cost-Effective Spinning Disk System for Live Imaging of Inner Ear Tissue

      • Federico Ceriani, Catalin D. Ciubotaru, Mario Bortolozzi, Fabio Mammano
      Pages 223-241
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    4. Neuroanatomical Tracing Techniques in the Ear: History, State of the Art, and Future Developments

      • Bernd Fritzsch, Jeremy S. Duncan, Jennifer Kersigo, Brian Gray, Karen L. Elliott
      Pages 243-262
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    5. Detection of Excitatory and Inhibitory Synapses in the Auditory System Using Fluorescence Immunohistochemistry and High-Resolution Fluorescence Microscopy

      • Wibke Singer, Hyun-Soon Geisler, Rama Panford-Walsh, Marlies Knipper
      Pages 263-276
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    6. Rapid and Semi-automated Extraction of Neuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks

      • Paul S. Holcomb, Michael Morehead, Gianfranco Doretto, Peter Chen, Stuart Berg, Stephen Plaza et al.
      Pages 277-290
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Editors and Affiliations

  • College of Medicine, University of South Florida, Tampa, USA

    Bernd Sokolowski

Bibliographic Information

  • Book Title: Auditory and Vestibular Research

  • Book Subtitle: Methods and Protocols

  • Editors: Bernd Sokolowski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3615-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3613-7Published: 04 June 2016

  • Softcover ISBN: 978-1-4939-8095-6Published: 26 May 2018

  • eBook ISBN: 978-1-4939-3615-1Published: 03 June 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 547

  • Number of Illustrations: 31 b/w illustrations, 89 illustrations in colour

  • Topics: Molecular Medicine, Human Genetics

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