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Rheumatoid Arthritis

Methods and Protocols

  • Book
  • © 2024
  • Latest edition

Overview

Editors:
  1. Shuang Liu

    1. Department of Pharmacology, Ehime University School of Medicine, Toon, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2766)

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About this book

This second edition explores standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols,  Second Edition aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study. 

Keywords

Table of contents (35 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Animal Models

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Collagen-Induced Arthritis Models

      • Maya Miyoshi, Shuang Liu
      Pages 3-7

    3. Human Xenograft Model

      • Shuang Liu
      Pages 9-15

    4. Scaffolded Chondrogenic Spheroid-Engrafted Model

      • Shuang Liu
      Pages 17-24

    5. Denervation-Induced Sarcopenia Model

      • Erika Takemasa, Shuang Liu
      Pages 25-30

    6. Long-Term Constant Subcutaneous Drug Administration

      • Shuang Liu, Maya Miyoshi
      Pages 31-36

    7. Clinical Scoring of Disease Activity in Animal Models

      • Maya Miyoshi, Shuang Liu
      Pages 37-42

    8. Histological Analyses of Arthritic Joints in Collagen-Induced Arthritis Model Mice

      • Takeshi Kiyoi
      Pages 43-53

    9. Preparation of Joint Extracts

      • Shuang Liu, Erika Takemasa
      Pages 55-60

  3. Therapeutic Approach

    1. Front Matter

      Pages 61-61
      Download chapter PDF

    2. Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System

      • Wei Zhou, Hiroyuki Takeda
      Pages 63-81

    3. Reconstruction of Protein/Liposome Complex

      • Yasuyuki Suzuki
      Pages 83-91

    4. Production of Neutralizing Antibody

      • Erika Takemasa, Shuang Liu
      Pages 93-106

    5. Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen

      • Hiroyuki Takeda
      Pages 107-128

    6. Generation of Specific Aptamers

      • Shuang Liu, Yasuyuki Suzuki, Makoto Inui
      Pages 129-137

    7. Detailed Protocol for Predicting 3D Structure of DNA Aptamers and Performing In Silico Docking Calculations

      • Yasuyuki Suzuki
      Pages 139-144

    8. RNA Interference Ex Vivo

      • Shuang Liu
      Pages 145-151

    9. Lentiviral-Mediated Systemic RNA Interference In Vivo

      • Shuang Liu
      Pages 153-161

    10. Lentiviral Production Platform

      • Shuang Liu
      Pages 163-168
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Editors and Affiliations

  • Department of Pharmacology, Ehime University School of Medicine, Toon, Japan

    Shuang Liu

Bibliographic Information

  • Book Title: Rheumatoid Arthritis

  • Book Subtitle: Methods and Protocols

  • Editors: Shuang Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3682-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3681-7Published: 26 January 2024

  • Softcover ISBN: 978-1-0716-3684-8Due: 08 February 2025

  • eBook ISBN: 978-1-0716-3682-4Published: 25 January 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 354

  • Number of Illustrations: 31 b/w illustrations, 52 illustrations in colour

  • Topics: Immunology, Behavioral Therapy

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