Abstract
Antibodies specifically recognizing integral membrane proteins are essential tools for functional analysis, diagnosis, and therapeutics targeting membrane proteins. However, developing antibodies against membrane proteins remains a big challenge because mass production of membrane proteins is difficult. Recently, we developed a highly efficient cell-free production method of proteoliposome antigen using a cell-free protein synthesis method with liposome and dialysis cup. Here, we introduce practical and efficient integrated procedures to produce a large amount of proteoliposome antigen for anti-membrane protein antibody development.
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Acknowledgment
The authors thank Mr. Tomio Ogasawara for his assistance in the technological development. They also thank Professor Tatsuya Sawasaki for his mentoring. This work was mainly supported by the Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED, Japan. This work was also partially supported by JSPS KAKENHI Grant Numbers 24710251, 26750375, and 16k01915.
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Zhou, W., Takeda, H. (2024). Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System. In: Liu, S. (eds) Rheumatoid Arthritis. Methods in Molecular Biology, vol 2766. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3682-4_9
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DOI: https://doi.org/10.1007/978-1-0716-3682-4_9
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