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Cardiac Tissue Engineering

Methods and Protocols

  • Book
  • © 2022
  • Latest edition

Overview

Editors:
  1. Kareen L.K. Coulombe

    1. School of Engineering, Brown University, Providence, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Lauren D. Black III

    1. Department of Biomedical Engineering, Tufts University, Medford, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2485)

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About this book

This detailed volume presents an updated collection of state-of-the-art protocols in cardiac tissue engineering. These protocols demonstrate advancements in cell sourcing, assembly, and use of engineered cardiac tissues, imaging and diagnostics, as well as therapeutic applications. New animal models, biomaterials, and quantitative analyses are described for broad adoption. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols, Second Edition serves as an ideal resource for inspiring the advancement of cardiotoxicity assessment, drug discovery, and heart repair and regeneration in order to accelerate heart health improvement around the globe.

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. CRISPR Library Screening in Cultured Cardiomyocytes

    • Sophia DeLuca, Nenad Bursac
    Pages 1-13

  3. Protein and mRNA Quantification in Small Samples of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates

    • Weizhen Li, Julie L. Han, Emilia Entcheva
    Pages 15-37

  4. Self-Assembled Heterotypic Cardiac Spheroids from Human Pluripotent Stem Cells

    • Oriane B. Matthys, Todd C. McDevitt
    Pages 39-53

  5. Acellular Myocardial Scaffolds and Slices Fabrication, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct

    • Bo Wang, Mickey Shah, Lakiesha N. Williams, Amy L. de Jongh Curry, Yi Hong, Ge Zhang et al.
    Pages 55-70

  6. FRESH 3D Bioprinting a Ventricle-like Cardiac Construct Using Human Stem Cell-Derived Cardiomyocytes

    • Brian D. Coffin, Andrew R. Hudson, Andrew Lee, Adam W. Feinberg
    Pages 71-85

  7. Engineered Heart Tissues for Contractile, Structural, and Transcriptional Assessment of Human Pluripotent Stem Cell-Derived Cardiomyocytes in a Three-Dimensional, Auxotonic Environment

    • Samantha Bremner, Alex J. Goldstein, Ty Higashi, Nathan J. Sniadecki
    Pages 87-97

  8. High-Throughput Analysis of Drug Safety Responses in Induced Pluripotent Stem Cell-Derived Cardiomyocytes Using Multielectrode Array

    • Nadjet Belbachir, Nathan Cunningham, Joseph C. Wu
    Pages 99-109

  9. iPSC-Derived Micro-Heart Muscle for Medium-Throughput Pharmacology and Pharmacogenomic Studies

    • Daniel W. Simmons, Nathaniel Huebsch
    Pages 111-131

  10. Quantifying Propagation Velocity from Engineered Cardiac Tissues with High-Speed Fluorescence Microscopy and Automated Analysis Software

    • Andrew P. Petersen, Megan L. McCain
    Pages 133-145

  11. Arrhythmia Assessment in Heterotypic Human Cardiac Myocyte–Fibroblast Microtissues

    • Celinda M. Kofron, Bum-Rak Choi, Kareen L. K. Coulombe
    Pages 147-157

  12. Human-Engineered Atrial Tissue for Studying Atrial Fibrillation

    • Julia Krause, Marta Lemme, Ingra Mannhardt, Alexandra Eder, Bärbel Ulmer, Thomas Eschenhagen et al.
    Pages 159-173

  13. Design and Fabrication of Biological Wires for Cardiac Fibrosis Disease Modeling

    • Erika Yan Wang, Jacob Smith, Milica Radisic
    Pages 175-190

  14. Methods for Transepicardial Cell Transplantation in a Swine Myocardial Infarction Model

    • Fanny Wulkan, Rocco Romagnuolo, Beiping Qiang, Michael A. Laflamme
    Pages 191-212

  15. Defined Engineered Human Myocardium for Disease Modeling , Drug Screening, and Heart Repair

    • Malte Tiburcy, Tim Meyer, Pierre-Luc Satin, Wolfram-Hubertus Zimmermann
    Pages 213-225

  16. Tubular Cardiac Tissue Bioengineered from Multi-Layered Cell Sheets for Use in the Treatment of Heart Failure

    • Hidekazu Sekine, Teruo Okano
    Pages 227-242

  17. Quantifying Cardiomyocyte Proliferation and Nucleation to Assess Mammalian Cardiac Regeneration

    • Emma B. Brandt, Ahmed I. Mahmoud
    Pages 243-253

  18. Injectable ECM Scaffolds for Cardiac Repair

    • Jervaughn D. Hunter, Todd D. Johnson, Rebecca L. Braden, Karen L. Christman
    Pages 255-268

  19. Encapsulation of Pediatric Cardiac-Derived C-Kit + Cells in Cardiac Extracellular Matrix Hydrogel for Echocardiography-Directed Intramyocardial Injection in Rodents

    • Preety Shakya, Milton E. Brown, Michael E. Davis
    Pages 269-278

  20. Characterization of the Monocyte Response to Biomaterial Therapy for Cardiac Repair

    • Sarah McLaughlin, David Smyth, Emilio I. Alarcon, Erik J. Suuronen
    Pages 279-298
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Editors and Affiliations

  • School of Engineering, Brown University, Providence, USA

    Kareen L.K. Coulombe

  • Department of Biomedical Engineering, Tufts University, Medford, USA

    Lauren D. Black III

Bibliographic Information

  • Book Title: Cardiac Tissue Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Kareen L.K. Coulombe, Lauren D. Black III

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2261-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2260-5Published: 28 May 2022

  • Softcover ISBN: 978-1-0716-2263-6Published: 28 May 2023

  • eBook ISBN: 978-1-0716-2261-2Published: 26 May 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 314

  • Number of Illustrations: 7 b/w illustrations, 78 illustrations in colour

  • Topics: Regenerative Medicine/Tissue Engineering, Physiology

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