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Human-Engineered Atrial Tissue for Studying Atrial Fibrillation

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Cardiac Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2485))

Abstract

This chapter details the generation of atrial fibrin-based engineered heart tissue (EHT) in standard 24-well format as a 3D model for the human atrium. Compared to 2D cultivation, human-induced pluripotent stem cells (hiPSCs)-derived atrial cardiomyocytes demonstrated a higher degree of maturation in 3D format. Furthermore, we have demonstrated in previous work that the model displayed atrial characteristics in terms of contraction and gene expression patterns, electrophysiology, and pharmacological response. Here, we describe how to embed atrial cardiomyocytes differentiated from hiPSCs in a fibrin hydrogel to form atrial EHT attached to elastic silicone posts, allowing auxotonic contraction. In addition, we describe how force and other contractility parameters can be derived from these beating atrial EHTs by video-optical monitoring. The presented atrial EHT model is suitable to study chamber-specific mechanisms, drug effects and to serve for disease modeling.

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Author information

Authors and Affiliations

  1. Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany

    Julia Krause, Marta Lemme, Ingra Mannhardt, Alexandra Eder, Bärbel Ulmer, Thomas Eschenhagen & Justus Stenzig

  2. DZHK (German Centre for Cardiovascular Research), Hamburg/Kiel/Lübeck, Germany

    Julia Krause, Marta Lemme, Ingra Mannhardt, Alexandra Eder, Bärbel Ulmer, Thomas Eschenhagen & Justus Stenzig

Authors
  1. Julia Krause
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  2. Marta Lemme
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  3. Ingra Mannhardt
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  4. Alexandra Eder
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  5. Bärbel Ulmer
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  6. Thomas Eschenhagen
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  7. Justus Stenzig
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Corresponding author

Correspondence to Thomas Eschenhagen .

Editor information

Editors and Affiliations

  1. School of Engineering, Brown University, Providence, RI, USA

    Kareen L.K. Coulombe

  2. Department of Biomedical Engineering, Tufts University, Medford, MA, USA

    Lauren D. Black III

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Krause, J. et al. (2022). Human-Engineered Atrial Tissue for Studying Atrial Fibrillation. In: Coulombe, K.L., Black III, L.D. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 2485. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2261-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2261-2_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2260-5

  • Online ISBN: 978-1-0716-2261-2

  • eBook Packages: Springer Protocols

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