Abstract
Extensive progress has been made in developing engineered models for elucidating human cardiac disease. Cardiac fibrosis is often associated with all forms of cardiac disease and has a direct deleterious effect on cardiac function. As currently there is no effective therapeutic strategy specifically designed to target fibrosis, in vitro diagnostic platforms for drug testing have generated significant interest. In this context, we have developed an innovative approach to generate human cardiac fibrotic tissues on Biowire II platform and established a compound screening system. The disease model is constructed to recapitulate contractile, biomechanical, and electrophysiological complexities of fibrotic myocardium. Additionally, an integrated model with fibrotic and healthy cardiac tissues coupled together can be created to mimic focal fibrosis. The methods for constructing the Biowire fibrotic model will be described here.
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Acknowledgments
We thank Drs. Yimu Zhao, Locke Davenport Huyer, and Boyang Zhang who established the initial methods of Biowire II platform design and polymer synthesis on which this chapter was based.
Some components of schematics were created with BioRender.com.
Radisic lab is funded by the Heart and Stroke Foundation Grant-in-Aid (G-16-00012711), Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant (RGPIN 326982-10), NSERC-CIHR Collaborative Health Research Grant (CHRP 493737-16), and National Institutes of Health Grant 2R01 HL076485. MR was supported by NSERC Steacie Fellowship and Canada Research Chair, LDH was supported by CIHR Vanier Scholarship. The authors acknowledge the Canada Foundation for Innovation, Project 19119, and the Ontario Research Fund for funding of the Centre for Spectroscopic Investigation of Complex Organic Molecules and Polymers.
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Wang, E.Y., Smith, J., Radisic, M. (2022). Design and Fabrication of Biological Wires for Cardiac Fibrosis Disease Modeling. In: Coulombe, K.L., Black III, L.D. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 2485. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2261-2_12
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DOI: https://doi.org/10.1007/978-1-0716-2261-2_12
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