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Receptor and Ion Channel Detection in the Brain

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  • © 2021
  • Latest edition

Overview

Editors:
  1. Rafael Lujan

    1. Instituto de Investigación en Discapacidades Neurológicas (IDINE), Departamento de Ciencias Médicas, Facultad de Medicina, Universidad Castilla-La Mancha, Albacete, Spain

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  2. Francisco Ciruela

    1. Pharmacology Unit, Department of Pathology and Experimental Therapeutics, School of Medicine and Health Sciences, IDIBELL, Universitat de Barcelona, L’Hospitalet de Llobregat, Barcelona, Spain

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 169)

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About this book

This second edition volume expands on the previous edition with new information on the latest developments in the field, and the most up-to-date representative methodological information on molecular neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters in this book are organized into three parts. Part One discusses molecular immunological and pharmacological aspects related to the detection of receptors and ion channels in the brain. Part Two explores the neuroanatomical techniques devoted to localizing receptors and ion channels within the central nervous system. Part Three looks at functional approaches devoted to ascertaining receptors and ion channels functionality in the brain. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.


Cutting-edge and comprehensive, Receptor and Ion Channels Detection in the Brain, Second Edition is a valuable resource that contains detailed molecular, cellular, neuroanatomical, and electrophysiological methods that will help researchers study neurotransmitter receptor and ion channel in any region of neuron of the brain.


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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xix
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  2. Molecular Techniques

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Production of High-Quality Antibodies for the Study of Receptors and Ion Channels

      • Masahiko Watanabe
      Pages 3-18

    3. Co-Immunoprecipitation from Brain

      • Xavier Morató, Dasiel O. Borroto-Escuela, Kjell Fuxe, Víctor Fernández-Dueñas, Francisco Ciruela
      Pages 19-30

    4. Subsynaptic Membrane Fractionation

      • Paula M. Canas, Rodrigo A. Cunha
      Pages 31-38

    5. Quantitative Analysis of Cell Surface Expressed, Intracellular, and Internalized Neurotransmitter Receptor Populations in Brain Slices Using Biotinylation

      • Elek Molnár
      Pages 39-54

    6. Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells

      • Juan Varela, Julien Dupuis, François Maingret, Laurent Groc
      Pages 55-66

    7. Radioligand Binding Detection of Receptors in Brain Membranes

      • Fuencisla Pilar-Cuéllar, Emilio Garro-Martínez, Elena Castro, Álvaro Díaz
      Pages 67-85

    8. Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain

      • Markus U. Ehrengruber, Kenneth Lundstrom
      Pages 87-106

    9. Time-Resolved Fluorescence Resonance Energy Transfer Using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain

      • Víctor Fernández-Dueñas, Thierry Durroux, Francisco Ciruela
      Pages 107-115

    10. Study of GPCR Homo- and Heteroreceptor Complexes in Specific Neuronal Cell Populations Using the In Situ Proximity Ligation Assay

      • Manuel Narváez, Minerva Crespo-Ramírez, Ramon Fores-Pons, Mariana Pita-Rodríguez, Francisco Ciruela, Malgorzata Filip et al.
      Pages 117-134

    11. Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Postmortem Brain

      • Marta Valle-León, Víctor Fernández-Dueñas, Francisco Ciruela
      Pages 135-142

  3. Neuroanatomical Techniques

    1. Front Matter

      Pages 143-143
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    2. Fluorescent In Situ Hybridization for Sensitive and Specific Labeling

      • Miwako Yamasaki, Masahiko Watanabe
      Pages 145-160

    3. Autoradiographic Visualization of G Protein-Coupled Receptors in Brain

      • Rebeca Vidal, Fuencisla Pilar-Cuellar, Alvaro Díaz, Elena Castro
      Pages 161-174

    4. Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains Using In Situ Immunoblotting

      • Elek Molnár
      Pages 175-190

    5. Immunohistochemistry for Ion Channels and Their Interacting Molecules: Tips for Improving Antibody Accessibility

      • Kohtarou Konno, Masahiko Watanabe
      Pages 191-199

    6. Localization of GFP-Tagged Proteins Under the Electron Microscope

      • Sara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez, P. García-Belda, S. Gil-García, M. Fil et al.
      Pages 201-212

    7. Tyramide Signal Amplification for Immunoelectron Microscopy

      • María José Ulloa-Navas, Patricia García-Tárraga, Susana González-Granero, Pedro Pérez-Borreda, Vicente Herranz-Pérez, José Manuel García-Verdugo
      Pages 213-222

    8. Pre-Embedding Methods for the Localization of Receptors and Ion Channels

      • Rafael Luján
      Pages 223-242
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Editors and Affiliations

  • Instituto de Investigación en Discapacidades Neurológicas (IDINE), Departamento de Ciencias Médicas, Facultad de Medicina, Universidad Castilla-La Mancha, Albacete, Spain

    Rafael Lujan

  • Pharmacology Unit, Department of Pathology and Experimental Therapeutics, School of Medicine and Health Sciences, IDIBELL, Universitat de Barcelona, L’Hospitalet de Llobregat, Barcelona, Spain

    Francisco Ciruela

Bibliographic Information

  • Book Title: Receptor and Ion Channel Detection in the Brain

  • Editors: Rafael Lujan, Francisco Ciruela

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-1522-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1521-8Published: 27 July 2021

  • Softcover ISBN: 978-1-0716-1524-9Published: 28 July 2022

  • eBook ISBN: 978-1-0716-1522-5Published: 26 July 2021

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 2

  • Number of Pages: XIX, 554

  • Number of Illustrations: 29 b/w illustrations, 80 illustrations in colour

  • Topics: Neurosciences

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