Abstract
Protein–protein interactions (PPI) play an important role in cellular functions and biological processes within the organism. G-protein-coupled receptors (GPCRs) form the largest group of the receptors involved in cell signaling across the plasma membrane, thus being the main pharmacotherapeutic target. Importantly, GPCRs can interact between them, a phenomenon known as GPCR oligomerization. In recent years, the characterization of direct PPI between GPCRs has gained interest, since these entities (i.e., GPCR oligomers) may evolve into important pharmacological targets in pathological conditions. Accordingly, novel techniques are needed, especially when aiming at revealing GPCR oligomerization in human tissue (i.e., postmortem brain), to monitor oligomer formation dynamics in disease. Here, we present a novel methodology to ascertain PPI between two GPCRs, which is the Amplified Luminescent Proximity Homogeneous Assay (Alpha) technique.
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Acknowledgments
This work was supported by Ministerio de Ciencia, Innovación y Universidades–Agencia Estatal de Investigación-FEDER-UE (SAF2017-87349-R MICIU/AEI/FEDER/UE), and the Catalan government (2017 SGR 1604). We thank Centres de Recerca de Catalunya (CERCA) Programme/Generalitat de Catalunya for IDIBELL institutional support. We thank E. Castaño and B. Torrejón from the Scientific and Technical Services (SCT) group at the Bellvitge Campus of the University of Barcelona for their technical assistance.
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Valle-León, M., Fernández-Dueñas, V., Ciruela, F. (2021). Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Postmortem Brain. In: Lujan, R., Ciruela, F. (eds) Receptor and Ion Channel Detection in the Brain. Neuromethods, vol 169. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1522-5_10
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DOI: https://doi.org/10.1007/978-1-0716-1522-5_10
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