Overview
- Provides a comprehensive overview of available techniques and methods for studying various types of DNA modifications
- Organized as detailed step-by-step protocols for experimental procedures required to successfully perform analysis
- Offers new opportunities for researchers to manipulate the modification status to affect gene expression, and develop small molecules to tune the DNA functions
Part of the book series: Springer Protocols Handbooks (SPH)
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About this book
Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years.
This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), β-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.
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Keywords
Table of contents (16 protocols)
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Detection of 5mC, 5hmC, 5fC, and 5caC
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Detection of 6mA
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Detection of 5hmU and 5fU
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Detection of Base J and 8-oxo-7,8-dihydroguanine
Editors and Affiliations
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Bibliographic Information
Book Title: DNA Modification Detection Methods
Editors: Bi-Feng Yuan
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-1-0716-1229-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-1228-6Published: 07 July 2021
Softcover ISBN: 978-1-0716-1231-6Published: 07 July 2022
eBook ISBN: 978-1-0716-1229-3Published: 06 July 2021
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: VI, 194
Number of Illustrations: 18 b/w illustrations, 30 illustrations in colour
Topics: Biomedical Engineering/Biotechnology, Gene Expression, Biological Techniques