Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2086)
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About this book
This volume provides comprehensive methods from expert scientists working in the Chimeric Antigen Receptor T Cell (CAR-T Cell) field. Chapters guide readers through the state-of-art of CAR-T cell technology, CAR design and vector production, CAR-T cell generation and manufacturing, CAR-T cell characterization, and quality control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Chimeric Antigen Receptor T Cells: Development and Production aims to be useful in the production of CAR-T cells, especially for therapeutic purposes.
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Keywords
Table of contents (20 protocols)
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Design of Chimeric Antigen Receptors and Vector Production
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CAR-T Cells Generation and Manufacturing
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CAR-T Cells Characterization and Quality Control
Editors and Affiliations
Bibliographic Information
Book Title: Chimeric Antigen Receptor T Cells
Book Subtitle: Development and Production
Editors: Kamilla Swiech, Kelen Cristina Ribeiro Malmegrim, Virgínia Picanço-Castro
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0146-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0145-7Published: 10 November 2019
Softcover ISBN: 978-1-0716-0148-8Published: 10 November 2020
eBook ISBN: 978-1-0716-0146-4Published: 09 November 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 274
Number of Illustrations: 4 b/w illustrations, 25 illustrations in colour
Topics: Cancer Research, Cell Biology, Immunology, Pharmacology/Toxicology