Abstract
Production of acetyl esterase by Termitomyces clypeatus was stimulated by xylan, cellulose, arabinose and arabinose-containing polysaccharides in the growth medium. The culture filtrate was equally active with p-nitrophenyl acetate and acetyl xylan. Acetyl xylan was completely deacetylated by the enzyme. Activity was optimum at pH 6.5 and at 50¡C. The Km values for p-nitrophenyl acetate and acetyl xylan were 0.83 mM and 0.38% (w/v) with Vm of 48 and 55 mmole acetate produced/min.mg protein, respectively.
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Mukhopadhyay, A., Hazra, P., Sengupta, T. et al. Acetyl esterase production by Termitomyces clypeatus. Biotechnology Letters 19, 159–162 (1997). https://doi.org/10.1023/A:1018316531658
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DOI: https://doi.org/10.1023/A:1018316531658