Abstract
Solanum chrysotrichum cell cultures were grown in a stirred tank bioreactor and their rheological and morphological behaviour were evaluated. The culture broths exhibited non-Newtonian and shear-thinning characteristics. Pseudoplasticity of the broths was governed by their biomass concentration. The roundness of aggregates measured as the elliptical form factor (EFF) had important changes. At the beginning of the culture the aggregates with an EFF lower than 2 represented 52% of the population, but in stationary phase the proportion increased to 77%. Whereas the size of aggregates did not change 80% of the population had an area lower than 0.1 mm2. Overall, these results indicate that the shape of the aggregate therefore needs to be considered when studying plant broth rheology.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Atkinson B,Mavituna F (1983) Biochemical Engineering and Biotechnology Handbook. London: MacMillan Publishers Ltd.
Curtis W,Emery A (1993) Plant cell suspension culture rheology. Biotechnol. Bioeng. 42: 520-526.
Jolicoeur M,Chavarie C,Carreau P,Archambault J (1992) Development of a helical-ribbon impeller bioreactor for high-density plant cell suspension cultures. Biotechnol. Bioeng. 39: 511-521.
Kato A,Kawazoe S,Soh Y (1978) Viscosity of the broth of tobacco cells in suspension culture. J. Ferment. Technol. 5: 224-228
Kieran P,MacLoughlin P,Malone D (1997) Plant cell suspension cultures: some engineering considerations. J. Biotechnol. 59: 39-52.
McDonald K,Jackman A,Hurst S (2001) Characterization of plant suspension cultures using the focused beam reflectance technique. Biotechnol. Lett. 23: 317-324
Miyanaga K,Seki M,Furusaki S (2000) Analysis of pigmentation in individual cultured plant cells using an image processing system. Biotechnol. Lett. 22: 977-981
Murashige T,Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15. 473-497.
Rodríguez-Monroy M,Galindo E (1999) Broth rheology, growth and metabolite production of Beta vulgaris suspension culture: a comparative study between cultures grown in shake flasks and in a stirred tank. Enzyme Microb. Technol. 24: 687-693.
Tanaka H (1982) Oxygen transfer in broths of plant cells at high density. Biotechnol. Bioeng. 24: 425-442.
Thomas C,Paul G (1996) Applications of image analysis in cell technology. Curr. Opin. Biotechnol. 7: 35-45.
Villarreal L,Arias C,Vega J,Feria-Velasco A,Ramírez O,Nasario P,Rojas G,Quintero R (1997) Large-scale cultivation of Solanum chrysotrichum cells: production of the antifungal saponin SC-1 in 10-l airlift bioreactors. Plant Cell Rep. 16: 653-656.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Trejo-Tapia, G., Jiménez-Aparicio, A., Villarreal, L. et al. Broth rheology and morphological analysis of Solanum chrysotrichum cultivated in a stirred tank. Biotechnology Letters 23, 1943–1946 (2001). https://doi.org/10.1023/A:1013789918463
Issue Date:
DOI: https://doi.org/10.1023/A:1013789918463