Abstract
To document in more details our knowledge of the fungal community associated to the Orchidaceae family, 25 cultivable fungal species were isolated from greenhouse apparently healthy Dendrobium fimbriatum var. oculatum Hook. adult plants. These fungi, were identified based on a molecular approach, belonging predominantly to Fusarium, Trichoderma, Colletotrichum, Curvularia and Didymella genera. To characterize their impact on plant growth and/or plant defense, their ability to produce different metabolites (phytohormones, siderophores) and hydrolytic enzymes, as well as their anti-phytopathogenic activity against Botrytis cinerea, Colletotrichum gloeosporioides and Fusarium oxysporum, were evaluated ex situ. Based on these criteria, three fungi, initially isolated from the roots, Fusarium sp., Trichoderma yunnanense, and Curvularia sp., showed a high potential to act on plant growth. Our results also show that an important number of isolates of the fungal community were able to produce phytohormones, siderophores, and had a similar hydrolytic enzyme production profile. Finally, these fungal abilities, studied ex situ, were analyzed to determine whether they can be used to classify the fungal partners as beneficial or detrimental to their host.
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1 Introduction
1.1 Dendrobium and microorganism’s relationships
Dendrobium is widely distributed in Asia. It is the second largest genus after Bulbophyllum in the Orchidaceae family with over 1500 species (Givnish et al. 2015). In Dendrobium genus, at least 16.4% of the species are endangered. This number is an estimation as conservation data is still missing for 28.8% of the species (International Union for Conservation of Nature red list). This genus contains the highest number of traditional vascular medicinal epiphytes (Nugraha et al. 2020). Indeed, Dendrobium species have been used as first-rate herbs and prized folk medicine in Asia for a thousand years (Ng et al. 2012; Cakova et al. 2017).
It was reviewed recently that 131 compounds from Dendrobium plants have been reported to possess anti-inflammatory, antimicrobial, antioxidant, antiaging, anti-psoriasis, and tyrosinase-inhibitory activities, highlighting this genera as an important resource for the development of new drugs and cosmetics (Wang 2021).
Dendrobium fimbriatum var. ocultatum, belonging to the so called “Fengdou Shihu” in traditional Chinese medicine (TCM), is found in Southeast Asia between 500 and 1500 m of altitude. In addition to the importance of the genus in traditional medicine, its flowers ranging from pale yellow to deep orange make of it economically valuable ornamental plant. Furthermore, because orchids have complex interactions with specific pollinating insects (Phillips et al. 2011), bacteria (Teixeira da Silva et al. 2015), and with diverse non-mycorrhizal and mycorrhizal endophytic fungi (Selosse 2014; Parthibhan et al. 2017; Novotná et al. 2018; Shah et al. 2019; Meng et al. 2019), they represent an interesting model to study plant-microbe interactions.
Dendrobium species are associated with their fungal community, made of diverse fungal partners (endophytes and epiphytes), that may have beneficial or detrimental effects on host plants. The term epiphyte described any organism which grows on the surface of a plant (Yao et al. 2019). The term endophyte, was first introduced by De Bary (Bary 1866), and applies to any organism found within a plant. The word’s meaning has since evolved to include all organisms inhabiting plant organs which, at some time in their life, can colonize internal plant tissues without causing apparent harm to their host (Petrini 1991). This definition of fungal endophytes sensu lato include consequently the “true” endophytes stricto sensu (Mostert et al. 2000), fungi whose colonization never results in visible disease symptoms, and the latent pathogens that may live symptomless in their hosts for some time in their life but may, at a given time, modify their behavior to become pathogens. Some true endophytes have been shown to have beneficial actions on host plants as growth-promoting effects (Shah et al. 2019), to increase host fitness, and to contribute to effective host defense against pathogens (Sarsaiya et al. 2020b; Kamel et al. 2020), herbivores, or abiotic stress (Yamaji et al. 2016). True endophytes may also increase or modulate the content of alkaloids, polysaccharides, saccharides and flavonoids in plants (Zhang et al. 2013; Tian et al. 2014; Taghinasab and Jabaji 2020). Finally, orchids fungal endophytes can be a sustainable source for the development of industrial and pharmaceutical important biomolecules (Sarsaiya et al. 2019b).
Despite the ecological and economical importance of orchids, the relationship with their mycobiome has been insufficiently studied. Furthermore, the molecular mechanisms involved in the establishment and maintenance of the endophytes-host plant relationships are far from being understood (Yan et al. 2019; Favre-Godal et al. 2020; Mattoo and Nonzom 2021). There is a lack of fundamental knowledge on the biochemical interactions between the fungal community and the plant host (Bünger et al. 2020; Trivedi et al. 2020). Such interactions have only been investigated so far for Dendrobium fimbriatum association with Xylaria species and mycorrhizal fungi (Chen et al. 2013; Xing et al. 2013; Soelistijono et al. 2020).
1.2 Goal of this work
In this study we identified the fungal community of healthy greenhouse D. fimbriatum adult plants and characterized the functional potential of each fungal partner on plant growth and/or plant defense.
The fungal community was isolated and identified by a similarity search of their internal transcribed spacers 1 and 2 plus the 5.8S (ITS) nuclear ribosomal DNA sequence with sequences deposited in GenBank (https://www.ncbi.nlm.nih.gov/genbank/).
To characterize the action of each isolate on the adult host plant, co-culture experiments with evaluation of the establishment and maintenance of the relationship, and the neutral/positive/negative effects on the host were the first-choice experiments. Usually, it is done by with protocorms/plantlets treated with fungal elicitor solution (supernatant of several days old fungal liquid culture) (Shah et al. 2019; Chand et al. 2020) or pure fungal culture (Sarsaiya et al. 2020b). Unfortunately, co-culture experiments can be challenging with the high numbers of fungal candidates to evaluate and the difficulties to obtain axenic plants as orchids tend to accumulate a high number of fungal endophytes. To overcome these issues, a preliminary ex situ evaluation of the ability of fungal isolates to produce different plant-microbe features such as metabolites or enzymes known to be involved in plant growth and/or plant defense (phytohormones, siderophores, antimicrobial and hydrolytic enzymes) as well as their anti-fungal activity on pathogenic fungal species of orchids (Botrytis cinerea, Colletotrichum gloeosporioides, and Fusarium oxysporum), was performed (Dolatabad et al. 2017; Bilal et al. 2018; Shah et al. 2019; Chand et al. 2020).
Finally, the characterization of the isolates as beneficial or detrimental partner based on these results and the limitation of such approach to select an appropriate fungal partner for further analysis were discussed.
2 Materials and methods
2.1 Isolation, identification, and molecular systematics of fungal isolates
Three commercial pot-cultured of Dendrobium fimbriatum var. oculatum Hook. were obtained from the Phrao Orchids Nursery green house in Chiang Mai, Thailand.
The cultivable part of the fungal community associated with these apparently healthy mature plants was isolated by sampling plant material from healthy roots, shoots, leaves and flowers. The fresh tissues, taken randomly from two pots, were first rinsed thoroughly under a gentle stream of tap water for 2 h (non-aggressive removal of surface particles without chemicals). Three samples per organs were then washed in four successive baths of sterile distilled water under a laminar flow hood during 5 min each, then sectioned (longitudinally) using a sterile blade. Squares of 9 mm2 were placed each on Petri dish containing potato dextrose agar (PDA) amended with chlortetracycline hydrochloride (25 mg/L; Sigma ref. 26,430).
Plates were inspected daily for the emergence of fungi over 3 weeks. Emerging fungi were isolated in pure culture and grown on PDA at room temperature. The isolated fungal strains are stored at 4 °C in the dynamic mycotheca of Agroscope (www.mycoscope.ch) in vials containing a diluted potato dextrose broth (PDB) solution in sterile water (1:4).
A sample of 0.5 cm3 of each fungal pure culture was placed in an Eppendorf tube containing 500 μl of cetyl trimethyl ammonium bromide buffer (CTAB 1x), and fungal genomic DNA extractions were performed following Inglis et al. (2018). Fungal strains were characterized by amplification and sequencing of their internal transcribed spacers 1 and 2 (ITS) plus the 5.8S of ribosomal DNA (rDNA), using the primers ITS1F and ITS4 (5‘-CTTGGTCATTTAGAGGAAGTAA-3′ and 5‘-TCCTCCGCTTATTGATATGC-3′ respectively); (http://sites.biology.duke.edu/fungi/mycolab/primers.htm). Polymerase chain reaction (PCR) used the following program: Initial denaturation at 95 °C for 2 min; 20 cycles including 30 s of denaturation at 94 °C, 30 s of primer annealing at 55 °C, 30 s of extension at 72 °C, and a final extension at 72 °C for 10 min. Purification and Sanger’s sequencing of the PCR products were performed by Fasteris Sa (Geneva, Switzerland; https://fasteris.com/). Sequencing was performed in both directions using the same primers as for amplification and using Sanger’s method. The obtained ITS sequences were deposited in GenBank.
Once trimmed, a first sequence similarity search (BLAST®; https://blast.ncbi.nlm.nih.gov/) in GenBank (National Center for Biotechnology Information; (Benson et al. 2018)) was performed using the “blastn” (Megablast) option excluding “uncultured/environmental sample sequences”. A second similarity search was done limiting the search to “sequences from type material”. When blast top scores were equally high for several GenBank sequences we favored sequences published in taxonomic studies and/or sequences that were obtained from type material. The method used for the identification of the fungal isolates followed (Hofstetter et al. 2019) with higher cut-offs.
ITS sequences were aligned in MacClade v. 4.08a (Maddison and Maddison 2000). Sequence alignment was performed with the MAFFT v.7.475 program, using default options (Kuraku et al. 2013). Maximum likelihood phylogenetic analyses were conducted in PhyML v. 3.0 (Guindon et al. 2010), with evolutionary model = GTR. Branch robustness was evaluated based on 500 bootstrap replicates, with the same settings as for the search for the most likely tree. Branch support was considered significant when bootstrap values were ≥ 70% (Alfaro et al. 2003).
2.2 Quantification of IAA in liquid medium
The production of IAA and IAA analogues (Indole pyruvic acid and Indole acetamide) by fungal isolates were determined spectrophotometrically with a 96 well plates method adapted from a previous study with Salkowski reagent (Gravel et al. 2007). The reagent is specific for IAA, but also for IAA analogues (Glickmann and Dessaux 1995). Fungal isolates were grown during one week in Petri dishes containing PDA. Liquid cultures were prepared by placing two 9 mm2 agar plugs of fungal pure cultures in 50 ml falcon tubes with 20 ml of PDB, with or without a 2 mg/ml supplement of L-tryptophan. Liquid cultures were done in triplicate. The inoculated broths were incubated in a shaker incubator at 20 °C, 180 rpm for 10 days, and then centrifuged twice at 9000 rpm for 10 min at 20 °C.
Twenty microliters of DMSO, 75 μl of supernatant and 150 μl of Salknowski reagent (49 ml of 70% perchloric acid, 49 ml of deionized water and 2 ml of FeCl3 at 0,5 M) (Gordon and Weber 1951) were mixed in a 96 well plate, and incubated in the dark for 30 min. The optic density was measured at 530 nm using an UV-VIS spectrophotometer (ChromTech-CT 8200). Absorbance of the empty plate was subtracted. The IAA concentration of the extract was quantified against a prepared standard IAA reference scale (1 to 100 μg/ml) constituted of 75 μl of liquid media, 150 μl of Salknowski reagent and 20 μl of IAA solutions in DMSO. Three biological replicates were performed for each experiment.
2.3 Evaluation of siderophore production
Production of siderophores was assessed by a modified chrome azurol S (CAS) method (Milagres and Machuca 1999). After solidification of PDA in Petri dish, the medium was cut into halves, one of which was replaced by CAS-blue agar (Lynne et al. 2011). The plates were inoculated individually on the PDA side with a fungal mycelial plug (9 mm2) and then incubated at 20 °C for 10 to 15 days, depending on the growth speed. Siderophore production was determined daily by measuring the appearance of the color change on the side containing the CAS-blue indicator. If a red color was observed, the isolate was considered as a producer of siderophores. Three inoculum replicates were made for siderophore production evaluation. Control plates without fungi were also assessed. Positive control was obtained with Escherichia coli (ATCC® 25922™).
2.4 Enzymatic profiling
The presence of 19 enzymes for each fungal isolate liquid culture has been confirmed by API-ZYM method (bioMérieux, Craponne, France). The 25 fungal isolates were grown in Petri dishes with PDA medium for one week. Liquid cultures were prepared by placing two 9 mm2 agar plugs of fungal pre-cultures in 50 ml falcon tubes with 20 ml of PDB. The inoculated broths were incubated in a shaker incubator at 20 °C, under an agitation of 180 rpm for 10 days. After incubation, they were ground with a homogenizer disperser ultra-turrax T25 (Janke & Kunkel®) at 8000 idle revolutions. One milliliter of the shred was resuspended in 4 ml of sterile distilled water and submitted to vortex at 180 rpm for 5 min.
Sixty-five microliters of the fungal suspension in sterile distilled water were added to each cupule of the strips. After incubation aerobically for 4 h at 37 °C in the dark and addition of manufacturer’s reagents (surfactant ZYM A and ZYM B from the API system) in each cupule, the color developed if an enzymatic reaction occurred. Enzymatic activities were classified according to the intensity of color with the API ZYM color reaction chart. All tested strains were examined twice to control the reproducibility of the results.
2.5 Antimicrobial assays
2.5.1 Confrontation assay in petri dish
The confrontation was performed by placing in a PDA Petri dish two 9 mm2 of agar plugs of one-week isolates fungal pre-cultures and two 9 mm2 of agar plugs of one-week fungal pre-cultures of phytopathogenic strain. The disposition of the plugs can be seen in Fig. 4a.
The experiment consisted of two Petri dishes replicates, represented by 25 isolates matched with three strains of phytopathogenic fungi (Botrytis cinerea MycoBank Agroscope GIK, Colletotrichum gloeosporioides MycoBank Agroscope 1015, and Fusarium oxysporum MycoBank Agroscope 238). The experiment was evaluated by observing the Petri dishes, using the “Interaction types” as defined by (Bertrand et al. 2013) for analyzing the proportion of antagonism between two paired fungi in culture medium.
2.5.2 Liquid-liquid extraction of fungal liquid culture
Twenty-five liquid cultures were prepared by placing 9 mm2 of agar plugs of one-week fungal pre-cultures in Erlenmeyer flasks containing 250 ml of PDB and incubating at 20 °C for 21 days. Mycelium was broken with a homogenizer disperser ultra-turrax T25 (Janke & Kunkel®) at maximum speed during 3 mins. Two cycles of liquid extraction were performed using ethyl acetate solvent in a 1:1 ratio of ethyl acetate (EtOAc): broth in the Erlenmeyer flask. The organic phase was collected, filtered, and evaporated using a rotary evaporator at 40 °C resulting in dry EtOAc extracts. The remaining aqueous phase was filtered consecutively with a cotton and a filter paper and finally lyophilized leading to dry aqueous extracts.
2.5.3 Micro-agar dilution method
EtOAc extracts were prepared to obtain three solutions at 40–20-10 mg/ml in DMSO. Aqueous extracts were prepared to obtain final concentrations of 20–10-5 mg/ml in water. Controls were generated in the same way with the commercial fungicide Switch (Cyprodinil 37.5% + Fludioxonil 25%, Syngenta) at 1 mg/ml. Ten microliters of commercial fungicide, 10 μl of EtOAc solutions or 20 μl of aqueous solutions were distributed into sterile 96-well microplates (Greiner, Frickenhausen, Germany). In each well with EtOAc or commercial solutions, 190 μl of PDA maintained at 45 °C was added. In each well with aqueous solution, 180 μl of PDA was added to reach a final volume of 200 μl per well. Each fungal extract was tested in triplicate.
Conidia of Botrytis cinerea were collected by vacuum aspiration from 10- day-old sporulating colonies using a filter tip and suspended in a sterile 50 ml Falcon tube containing sterile ultrapure water. The concentration was adjusted to 2 × 106 conidia/ml. Ten microliters of this conidial suspension were added to each well. Same protocol was used for the two other phytopathogenic fungi tested, Fusarium oxysporum and Colletotrichum gloeosporioides.
The plates were incubated in a growth chamber (60% relative humidity, constant temperature of 21 °C, alternating 16 h day and 8 h night cycles). If no mycelium were observed after 8 days, the extract was considered fungistatic at the concentration tested.
3 Results and discussion
3.1 Molecular identification of isolated fungi
A total of 25 fungal strains were recovered by our isolation procedure from different organs. After molecular identification of the fungal strains isolated by BLAST in GenBank (Table 1), the high majority of the strains belonged to Ascomycota (22) while the three remaining isolates were identified as Mucoromycota (genus Mucor [1 isolate] and Basidiomycota (order Agaricales, genus Coprinopsis [1] and order Tremellales, genus Papiliotrema [1]). Within Ascomycota, 10 strains were Pleosporales (genera Curvularia [4 isolates], Dydimella [2], Dydimocyrtis [1], Alternaria [1], Exserohilum [1], and Pseudopithomyces [1], 5 strains were Hypocreales (genera Fusarium [3], and Trichoderma [2], and 4 strains Glomerellales (genera Colletotrichum [3] and Plectosphaerella [1]. The three remaining isolates belonged to Sordariales (genus Neurospora [1]) and Eurotiales (genus Penicillium [2]). Those isolates are in accordance with the literature as Ascomycota and Basidiomycota are the phyla representing the majority of fungi found aboveground and belowground plant tissues (Trivedi et al. 2020). Compared to stems, leaves, and flowers, more fungi were found in roots. Indeed, the Curvularia, Coprinopsis, Mortierella, one Trichoderma, one Penicillium and one Fusarium were isolated from the roots. Isolates with the code OE-RLG were found only in the flowers (Table 1 and Fig. 1). Seven isolated strains (OE 1, OE-2, OE-4, OE-8, OE-27, OE-RLG4 and OE-RLG7) were identified only at the genus level mostly because ITS does not allow to distinguish between very closely related species within species complexes in genera Alternaria, Curvularia, Fusarium, Neurospora and Penicillium but also because of poor taxon coverage for Coprinopsis (Table 1 and Fig. 1).
Regarding the literature, those genera are common endophytes and have been described previously in Dendrobium species (XiQing et al. 2008; Chen et al. 2013; Xing et al. 2013). Fusarium, Phoma and Epicoccum were the dominant isolated endophytes genus in Dendrobium devonianum and D. thyrsiflorum (Xing et al. 2011). From these two orchid species, Phoma and Rhizopus were also isolated. Fusarium was also the dominant genus isolated from the roots of D. moniliforme, followed by Colletotrichum sp., Trichoderma sp., Hypoxylon sp., Cylindrocarpon sp. and Leptosphaerulina sp. (Shah et al. 2019). Colletotrichum strains were also isolated from three Dendrobium species: D. cariniferum, D. catenatum and D. harveyanum. Noticeably, it seems that leaves contained the higher number of Colletrotrichum species compared to the roots and stems (Ma et al. 2018). Similar observation was made during this study with Colletotrichum cf. cobbittiense (OE-71) and C. phyllanthi (OE-72) isolated from the leaves. In addition to the genera isolated in this work, xylariaceous fungi, corresponding to non-mycorrhizal fungi, were found abundant and diverse in the roots of several Dendrobium species (D. nobile; D. faiconeri; D. chrysanthum; D. aphyllum; D. crystallinum; D. fimbriatum) (Chen et al. 2012, 2013).
No mycorrhizal fungi were isolated due to isolation procedure. In other works, Tulasnellaceae were mainly isolated from identified mycorrhizal orchid roots of D. fimbriatum and D. nobile. In some plants, few members of the Ceratobasidiaceae and Pluteaceae were also identified (Xing et al. 2013).
Depending on the cultivation procedure (or environmental conditions), the plant life stage, the fungal pool available and the isolation procedures, a different fungal community can be found for a same orchid species (Jia et al. 2016; Jacquemyn et al. 2016; Dastogeer et al. 2020). Therefore, the relatively few isolates described here are obtained from a healthy adult plant cultivated under specific growth conditions and might not be similar for other plant individuals. However, the universality of those genera and their reports among Dendrobium genus suggest that they may be common among cultivated Dendrobium adult plants.
3.2 Quantification of indole acetic acid (IAA) synthetized by isolated fungi
IAA is the main auxin hormone in plants, having a central role in the regulation of many growth, development, and defense related processes (Davies 2010; Spaepen and Vanderleyden 2011). Phytohormones are key signaling molecules in plant-microbe interaction as they modulate and coordinate cellular and metabolic plant responses associated to the progression of microorganisms across tissues (Boivin et al. 2016; Mehmood et al. 2019). This explain partially why yeast isolated from plants showed higher auxin production compared to the one isolated from the soil (Streletskii et al. 2016).
Production of IAA and IAA analogues from tryptophan independent or dependent pathways were estimated for the fungi isolated from D. fimbriatum. Production of IAA without the amino acid addition was at too low concentrations to be detected or considered significant in all isolates (data not shown). However, the indole acetic acid concentration was higher in broths supplemented with tryptophan for all isolates indicating a tryptophan dependent biosynthetic pathway (Fig. 2a).
In this assay, Colletotrichum cobbittiense (OE-71) produced very high concentration of IAA and IAA analogues (841 μg/ml ±66), over 11-fold higher than (OE-25) or other isolates. It was necessary to dilute the sample to be in the same absorbance range of the standard curve. In a previous study, fermentation of C. siamense in PDB media led to the isolation of plant growth hormone IAA (Munasinghe et al. 2017). Noticeably another species of Colletotrichum, C. alatae, isolated from the roots of D. moniliforme, was the highest IAA producer in supplemented tryptophan media among the strains tested (Shah et al. 2019). Although OE-71 was originally isolated from the leaves, it does not exclude its presence in the roots, where at least for the Angiosperms, auxin hormones induce the formation and the outgrowth of branching roots.
OE-2, OE-4, OE-5, OE-8, OE-15, OE-20, OE-23, OE-25, OE-26, OE-27, OE-85 and RLG6 produced relatively high amount of IAA and IAA analogues, their average production ranging from 5 to 75 μg/ml. Therefore, most members of the fungal community of D. fimbriatum appears to be able to produce IAA and/or IAA analogues. However, some error bars are relatively important due do the variability in the plug agar inoculum per biological replicate.
Production of IAA by similar genus was described in previous published works. For example Trichoderma atroviride produced 0.5 and 6.2 μg/ml in medium supplement with tryptophan (Gravel et al. 2007; Colla et al. 2015). Fusarium sp. produced between 20 and 45 μg/ml in medium supplement with tryptophan depending on the Fusarium isolates (Shah et al. 2019).
Microbial IAA production is considered key in growth promoting activities of beneficial microbes (Khan et al. 2016; Bilal et al. 2018). For example, Paenibacillus strains isolated from the meristem of in vitro Cymbidium eburneum orchids enhanced the survival and promoted the growth of germinated Cattleya loddigesii seedlings, as well as increased the total biomass and number of leaves (Faria et al. 2013). Inhibition of IAA production by application of yucasin (a potent inhibitor of YUCCA, a key enzyme in auxin biosynthesis) reduced the colonization of Aspergillus awamori endophyte in maize roots by 52% when applied on the leaves and 66% when applied on the roots. The application of IAA restored the ability of Aspergillus awamori to colonize maize roots and significantly improved various growth parameters (Mehmood et al. 2019).
On the other hand, microbial IAA may act as a virulence factor during disease development (Fu et al. 2015). Indeed, microbial IAA can stimulate plant cell growth during infection, suppress host defenses and regulate virulence gene expression (Kunkel and Harper 2018; Djami-Tchatchou et al. 2020). IAA can be synthesized from tryptophan via the intermediate indole-3-acetamide (IAM) or via the intermediate indole pyruvic acid (IPA). IAM pathway was found in the cereal rust pathogen Puccinia graminis f. sp. tritici. and demonstrated by means of transient silencing that one gene of the pathway is required for full pathogenicity (Yin et al. 2014). Transgenic fungal pathogens of Orobranche aegyptiaca, Fusarium oxysporum and F. arthrosporioides overproducing IAA have enhanced virulence, which results in a decrease in the number and size of plant shoots (Cohen et al. 2002). Strikingly, the highest producer of IAA in this assay, OE-71, is from the genera Colletotrichum, from which several species were described as important phytopathogens of orchids (Kadir et al. 2020; Park et al. 2020). OE-71 was obtained from the leaves of D. fimbriatum, parts of the plant where the anthracnose disease is predominantly observed.
All those data support a primordial role of microbial IAA in plant-microbe interaction (beneficial or detrimental), particularly at the colonization step leading to plant growth stimulation or modification of basal plant defense mechanisms (Goyal and Kalia 2020).
3.3 Production of siderophores by isolated fungi
Siderophores are low molecular-weight compounds (less than 1 kDa), that have high affinity for ferric iron. Those iron-chelating molecules are produced by numerous organisms to sequester preferably iron or other metals from the environment allowing their future use for correct development. Siderophores are important in various ecological phenomena including iron biogeochemical cycling in soils, pathogen competition, plant growth promotion and regulation, communication, virulence, and oxidative stress processes during plant-microbe interaction (Albelda-Berenguer et al. 2019; Trivedi et al. 2020).
As siderophore production strongly coincides with a plant-associated lifestyle (Stringlis et al. 2018), extracellular siderophore secretion by isolated fungi have been measured by the CAS-blue agar assay (Fig. 3 and Online Resource 1).
In the present study, fifteen out of the 25 fungal species isolated from Dendrobium fimbriatum plants produced siderophores (OE-1, 3, 4, 5, 8, 17, 20, 23, 26, 28, 69, 85, RLG-7 and RLG-8) (Online Resource. 1 and Fig. 3). Fusarium cf. foetens (OE-85), Fusarium sp.2 (OE-18) and Penicillium cf. citreosulfuratum (OE-28) were clearly identified as siderophore producers due to a strong CAS media coloration. Species of the genus Fusarium and Trichoderma were previously designated as siderophore producers (Dolatabad et al. 2017; Bilal et al. 2018), whereas for Penicillium spp., siderophore production has been shown to be strain dependent (Shi et al. 2017). Not surprisingly, the strongest producers were isolated from the roots of D. fimbriatum, however, over half of the isolates in this work are producing extracellular siderophores, suggesting siderophore production a common ability for plant-associated microbes. No phylogenetic placement links could be made in this study. Curvularia eragrostidus or Fusarium sp. 2 did not produce siderophores whereas other species from the same genus did.
Microbial siderophore production can confer plant resistance to biotic stress. They can alter the microbial community by resource-competition (Kramer et al. 2020) and induction of the plant induced systemic resistance that would control the growth of soil-borne pathogens neighbors (Kundan et al. 2015; Igiehon and Babalola 2018). In parallel, siderophores reduce the plant oxidative stress, facilitate plant iron acquisition (Bar-Ness et al. 1992; Dellagi et al. 2009) and so confer resistance to abiotic stress (Choudhary et al. 2016; Kowalczyk and Latowski 2018). Consequently, they would promote plant growth as iron deficiency in plants causes chlorosis and growth defects (Hindt and Guerinot 2012; López-Millán et al. 2013). Such siderophore producing endophytic fungal strains are consequently considered as beneficial for plant health (Yamaji et al. 2016).
However, siderophores also play a pathogenesis effector role (Ruddat et al. 1991; Haas et al. 2008; Taguchi et al. 2010; Aznar and Dellagi 2015; Kramer et al. 2020). For example, mutants of Colletotrichum graminicola (maize pathogen), unable to produce siderophores were deficient in vegetative growth under iron-limiting conditions, conidiation, reactive oxygen species tolerance, cell wall integrity, and are though not virulent (Albarouki et al. 2014). Siderophore production is considered an essential virulence factor for some pathogenic species (Ruddat et al. 1991; Haas et al. 2008; Taguchi et al. 2010; Aznar and Dellagi 2015; Kramer et al. 2020).
Despite the clear involvement of siderophores in plant-microbe interaction and the high number of producers among the fungal community studied here, the incomplete understanding of siderophore production and utilization in such interaction prevent the direct description of siderophore-producers as beneficial or detrimental.
3.4 Anti-phytopathogenic activity of isolated fungi
Several fungal phytopathogens are reducing the quality and survival of Orchidaceae plants. Botrytis cinerea is responsible of botrytis petal blight, which is one of the most destructive and economically important diseases affecting greenhouse grown ornamental plants (Bika et al. 2020). Colletotrichum gloeosporioides causes anthracnose and has been significant in Dendrobium production losses (McMillian Jr 2011). Finally, species of the Fusarium complexes such as oxysporum, proliferatum, solani, subglutinans and fractiflexum, are reported to cause foliar and root diseases (Fusarium wilt) on Dendrobium, Cymbidium, Phalaenopsis and Cattleya plant species (Srivastava et al. 2018). The number of Fusarium diseases in orchids is increasing, leading to consider pathogenic Fusarium spp. as a major limiting factor for the production of high quality orchids (Srivastava et al. 2018; Jain et al. 2021).
To evaluate the anti-phytopathogenic activity of the fungi isolated in this work, confrontation assays in Petri dish with each isolate versus a main orchid phytopathogen (Botrytis cinerea, Colletotrichum gloeosporiodes and Fusarium oxysporum) were performed (Fig. 4a). In addition, their ethyl acetate (EtOAc) and aqueous extracts obtained by liquid-liquid extraction of a liquid culture were tested by micro-agar dilution method against those same three main orchid phytopathogens (Fig. 4b).
The EtOAc extract of both Trichoderma sp. (OE-5 and OE-15, Table 1) and of two Fusarium isolates (OE-2 and OE-18) had anti-phytopathogenic fungal activities against all the three species tested (Fig. 4c). The EtOAc extract of Fusarium sp. 1 (OE-4) had activity against Botrytis cinerea and Colletotrichum gloeosporioides but no activity against Fusarium oxysporum.
Those results are consistent with the literature as Trichoderma species, and to a smaller extent, non-pathogenic Fusarium, are known for their anti-phytopathogenic fungal activities due to their secondary metabolites, plant growth promotion, and local, as well as systemic, induced resistance (Al-Ani 2019; Saravanakumar and Wang 2020; Khan et al. 2020). For example, dendrobine, an alkaloid initially found in D. nobile, was isolated from T. longibrachiatum and had antibacterial activity against Bacillus subtilis, Bacillus mycoides, and Staphylococcus species (Sarsaiya et al. 2020a). Harzianic acid, a tetramic acid compound from T. harzianum, showed antifungal activity against Pythium irregulare, Sclerotinia sclerotiorum, and Rhizoctonia solani (Vinale et al. 2009) as well as plant growth promotion activities, probably due to its Fe(III)-binding activity (Vinale et al. 2013).
All the Curvularia EtOAc extracts had no anti-phytopathogenic fungal activities except for one Curvularia isolate (OE-8) extract, active against B. cinerea and C. gloeosporioides.
Didymella pedeiae (OE-17) EtOAc extract had strong anti-phytopathogenic fungal activity (0.5 mg/ml) against B. cinerea (Fig. 4c).
The aqueous extracts were inactive at the concentration tested. This is probably due to the low quantity of fungal secondary metabolites recovered compared to the culture media ingredients remaining in the aqueous phase.
Regarding the confrontation assays, all Trichoderma strains grown completely over the phytopathogenic fungi covering the surface of the medium whereas the Fusarium strains showed a contact inhibition profile with no dominance over the pathogenic species (Fig. 4c). Trichoderma after a week invaded and sporulated over the phytopathogen revealing its mycoparasitism (Sood et al. 2020).
For a better observation of the physical interactions of the hyphae, the replication of this confrontation assay at a smaller scale on a microscope slide covered in a thin layer of nutrient agar medium would be interesting (Hajieghrari and Giglou 2008; Djami-Tchatchou et al. 2020).
The EtOAc extract of OE-17 was inactive against Fusarium oxysporum but when OE-17 is in co-culture with this phytopathogen it was able to partially inhibit its growth. It might be due to induced antifungal mechanisms. In addition to constitutive antifungal productions, co-culture can induced the production of antimicrobials (Deshmukh et al. 2018; Arora et al. 2020; Christiansen et al. 2020). As micro-agar dilution assay does not measure such mechanism, the use of each methodology is complementary for our primary screening.
Only few fungi were able to inhibit the growth of the pathogenic strains studied here. This experiment is therefore interesting to evaluate and differentiate the functional potential of each isolate. Noticeably, isolates with anti-phytopathogenic activity were obtained from the leaves and roots, which are parts often exposed to fungal diseases, suggesting their beneficial impact for the host plant.
3.5 Secretion of hydrolytic enzymes by isolated fungi
Most fungal partner have the genetic capacity to produce enzymes (cellulases, xylanases and pectinases) that degrade the major structural polysaccharides found in the cell wall (cellulose, xylan and pectin) in order to enter the host cell (King et al. 2011).
The capabilities of fungal endophytes to produce specific enzymes can consequently be used for assessing their metabolic diversity, giving indication on their ecological role.
API-ZYM galleries were designed for semi-quantitative test of 19 constitutively expressed lipid, protein and carbohydrate-degrading enzymes of non-pretreated bacteria (e.g. Humble, King and Phillips 1977). Relatively few studies investigated the enzymograms of filamentous fungi using this technique (Bidochka et al. 1999; Tekere et al. 2001; Knapp and Kovács 2016; Żukiewicz-Sobczak et al. 2016). Cleverly, Knapp and Kovács (2016) used API-ZYM system to study interspecific metabolic diversity and functional heterogeneity of roots non-sporulating endophytic fungi. An adapted version of their protocol was used to highlight similarities or differences in enzymes production and therefore in their ecological functions (Fig. 5).
The production of hydrolytic enzymes was slightly variable among the fungal isolates but appeared to be unlinked to their phylogenetic placement or their origin tissue. In general, similar results with (Knapp and Kovács 2016) were obtained. Almost no trypsin (except for OE-23), lipase (except for OE-27), α-fucosidase, α-mannosidase, chymotrypsin and cystine arylamidase activities were detected from hyphal fragments and extracellular medium of the tested fungi.
In opposition, activities of esterase (C4), esterase lipase (C8), acid and alkaline phosphatase and β-glucosidase were strong with a clear detection for almost all isolates (Fig. 5a). All enzymograms were quite similar except for Mortierella aff. Sarnyensis. (OE-25) and Didymella pedeiae (OE-17) that produced fewer different enzymes compare to other members of the fungal community.
Species with esterase, cellulase, amylase, protease acid and alkaline phosphatase enzyme activities might play a role in the promotion of plant growth by decomposing organic matter, increasing phosphate availability and nutrient mineralization (Gianfreda 2015). They can also act as biocontrol agents for pathogens or stimulators of the plant immune system due to the release of partially enzymatic degraded wall constituents (Lionetti and Métraux 2014). The degradation products of the major structural polysaccharides found in the cell wall might also be used as nutrients because endophytes can become saprobes after the cell plant death or pathogens, depending on the plant-host conditions (King et al. 2011).
In addition to the nutrition role of phosphatase enzymes, it has been suggested that they also play important roles in proliferation, differentiation, adhesion, virulence and plant-infection (Freitas-Mesquita and Meyer-Fernandes 2014). For example, Cdc14 protein phosphatases play an important role in plant infection by several fungal pathogens such as Aspergillus flavus (Yang et al. 2018).
The ability to produce hydrolytic enzymes is common between beneficial, saprobes and detrimental fungi but the substrates specificities and kinetics might differ. Furthermore, fungal enzymes production is a substrate induced and dependent process. The enzymograms obtained in this work are consequently partial, and D. fimbriatum fungal community might produce several other enzymes of interest not evaluated in this preliminary study (like cutinase and lignin-modifying enzymes for example). API ZYM test remains a starting procedure to quickly study some enzymes production capability of fungi, but further analyses are needed as it could not help us to differentiate and characterize the ecological functions of the isolates studied here.
3.6 Characterization of isolated fungi as beneficial or pathogenic strains
It is important to notice that antimicrobials, phytohormones, siderophores and hydrolytic enzymes production are mechanisms used by both beneficial and pathogenic strains (cf. results discussion). Furthermore, the establishment of both plant-beneficial and plant-pathogenic microbe interactions, and the molecular communication strategies involve a balance of multiple factors which are often overlapping (Stringlis et al. 2018; Favre-Godal et al. 2020; Trivedi et al. 2020). In addition to the complexity of such interactions, general fungi of the mycobiome can easily shift from mutualist to parasite depending on the plant’s health and environmental conditions, requiring precaution in the description of beneficial partner (Tejesvi and Pirttilä 2018; Sarsaiya et al. 2019b; Jain et al. 2019). This is probably why, during experimental inoculations, the influence of a fungal root endophyte on the host plant could be controversial, with plant responses ranging from negative to positive (Mayerhofer et al. 2013).
It is then not surprising to see some Trichoderma species, frequently used as biological control agents and biofertilizers to control plant diseases and enhance crop yields (Alfiky and Weisskopf 2021) while others are described as pathogens (Li Destri Nicosia et al. 2014), with T. longibrachiatum causing black circular spots on Dendrobium nobile leaves (Sarsaiya et al. 2019a). Another example could be Fusarium spp., some are described as endophytes with beneficial effects (Hiruma et al. 2016; Hiruma 2019), like F. oxysporum reducing disease caused by vascular pathogens such as Verticillium dahliae and conferring protection against root pathogens like Pythium ultimum (de Lamo and Takken 2020), whereas the same species, F. oxysporum is an important fungal pathogen of orchids.
For all of those reasons, the plant-microbe features studied here should not be considered alone, out of a specific interaction, to categorize a strain of the mycobiome as beneficial or detrimental but rather used to characterize and select fungi with important plant-lifestyle behavior for further considerations.
In this context epiphytes, “true endophytes” regarding the definition of (Mostert et al. 2000) and probably latent pathogens have been isolated during this work. To differentiate epiphytes and endophytes, a stronger asepsis protocol can be performed. To differentiate “true endophytes” and latent pathogens, the plant-microbe features studied here are only preliminary results, needed to be complemented. To date, to unambiguously categorize isolated fungal orchids partners from latent pathogens to mutualistic symbionts, phenotypic evaluations with co-cultures experiments are still necessary (Sarsaiya et al. 2020b).
With the increasing knowledge on effector genes, comparing the genomes of the isolates and their putative effector genes (coding for putative host-specific virulence proteins), could become a strategy to differentiate beneficial to latent pathogens of a large set of fungal isolates. Indeed, endophytes, even if they have a common set of conserved putative effector genes with pathogenic strains, they appear to carry a fewer number and almost no host-plant specific effectors (Dam et al. 2016).
In the present study, three isolates out of twenty-five produced significantly all the evaluated plant-microbe features. Fusarium sp. (OE-4), Trichoderma yunnanense (OE-5), and Curvularia sp. (OE-8) are the fungal isolates from D. fimbriatum producing all siderophores, phytohormones and anti-phytopathogenic compounds (Fig. 6).
Noticeably, they were isolated from the roots, where plant-microbe interactions have been extensively documented. Those results designate them as fungal partners having potentially, strong interactions with the host plant, making of them candidates for further integrative approaches such as experimental modelling through co-cultivation experiments.
Data availability
ITS sequences can be found in Genbank with the accession’s numbers of Table 1. The fungal strains isolated are stored at 4 °C in the dynamic mycotheca of Agroscope (www.mycoscope.ch).
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Acknowledgments
We would like to thank Léa Mazina for her logistic support, Marine Bellanger for her proofreading, and Eric Remolif and Sylvia Dusonchet for their expertise and work on orchid growth.
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This research was financially supported by Guerlain to contribute in the understanding of microbe-plant relationship and preservation of orchids.
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Favre-Godal, Q., Schwob, P., Lecoultre, N. et al. Plant-microbe features of Dendrobium fimbriatum (Orchidaceae) fungal community. Symbiosis 85, 31–46 (2021). https://doi.org/10.1007/s13199-021-00786-0
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DOI: https://doi.org/10.1007/s13199-021-00786-0