Abstract
Storage lipid and protein breakdown in germinating seeds of yellow (Lupinus luteus L.), white (L. albus L.), and Andean lupine (L. mutabilis Sweet) and regulatory function of sucrose were investigated. Less oil bodies were detected in organs of yellow lupine seeds, whereas the highest content of oil bodies was noticed in the Andean lupine seeds. Mature, air-dried yellow, white and Andean lupine seeds do not contain starch. Starch grains appear the earliest in white lupine seeds during imbibition. Sucrose deficiency in tissues enhances breakdown of storage lipid, protein and temporary starch in cotyledons. In sucrose starved embryo axes of all investigated lupine species, an increased level of vacuolization was noted. Interconnections between catabolism of storage protein and storage lipid in germinating lupine seeds were identified by applying 14C-acetate. To assess the importance of key processes in storage lipid breakdown NaF (inhibitor of glycolysis and gluconeogenesis), KCN, NaN3 and SHAM (inhibitors of mitochondrial electron transport chain) and MSO (inhibitor of glutamine synthetase) were used. Radioactivity coming from 14C-acetate was released as 14CO2 but mostly was incorporated into ethanol-soluble fraction of embryo axes and cotyledons. Respiratory inhibitors caused a significant decrease in 14CO2 and ethanol fractions in all three lupine species studied. MSO stimulated release of 14CO2 and radioactivity of ethanol fractions in yellow lupine organs fed with sucrose, but in Andean lupine MSO enhanced the production of 14CO2 and radioactivity of ethanol fractions both in organs fed and not fed with sucrose. Different strategies of storage compound breakdown are proposed, depending on relative proportion in storage protein and lipid content in lupine seeds.
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Introduction
Numerous research studies regarding storage lipid catabolism are conducted on germinating oil-storing seeds or young seedlings. During germination, lipid stored in oleosomes is used both as respiratory substrate and is converted into sugars (Graham 2008). In classical pathway of storage lipid conversion into sugars, the following sequence of metabolic events takes place: lipolysis, fatty acids β-oxidation, glyoxylate cycle, TCA cycle and gluconeogenesis (Fig. 1). This sequence of events was proposed by Beevers (1976, 1979) based on the results of experiments done on young castor bean seedlings and has been confirmed later in other oilseed plants (see reviews of Eastmond and Graham 2001; Penfield et al. 2005, 2006; Graham 2008; Quettier and Eastmond 2009; Kaur et al. 2009). Modifications of the process as described above are known; the examples are Arabidopsis mutants with disruptions in glyoxylate cycle (Eastmond et al. 2000; Cornah et al. 2004; Pracharoenwattana et al. 2005) or flow of lipid-derived carbon into developing chloroplast in Arabidopsis (Penfield et al. 2004). Moreover, some data indicate that fatty acid β-oxidation could be a source of intermediates for phytohormone (indole-3-acetic acid) and the signal molecule (jasmonic acids) synthesis (Baker et al. 2006; Nyathi and Baker 2006; Penfield et al. 2006; Poirier et al. 2006; Kaur et al. 2009).
Glyoxylate cycle is catalyzed by five key enzymes. Two of them, isocitrate lyase (ICL) and malate synthase (MLS), are “hallmark” enzymes for glyoxylate cycle, whereas citrate synthase (CSY), aconitase (ACO) and malate dehydrogenase (MDH) take part in the TCA cycle as well (Graham 2008; Pracharoenwattana and Smith 2008). However, it is known that not all glyoxylate cycle enzymes are located in glyoxysomes. For example, ACO is active in the cytosol and mitochondria but not in glyoxysomes (Courtois-Verniquet and Douce 1993; De Bellis et al. 1994; Hayashi et al. 1995; Eastmond and Graham 2001). MDH is located in glyoxysomes and is active there, but the glyoxysomal fraction of this enzyme does not participate directly in the glyoxylate cycle. Glyoxysomal MDH (gMDH) is involved in β-oxidation of fatty acids to re-oxidize NADH. The activity of the cytosolic MDH (cMDH) fraction is necessary for full operation of the glyoxylate cycle (Pracharoenwattana et al. 2007; Graham 2008; Pracharoenwattana and Smith 2008) (Fig. 1). Location of two enzymes of glyoxylate cycle in cytosol requires export of intermediates from glyoxysomes to cytosol and their subsequent import from cytosol into glyoxysomes. Citrate is exported to cytosol and after conversion to isocitrate by ACO it is imported into glyoxysomes. There, malate is synthesized in two steps catalyzed by ICL and MLS and transported into cytosol. From malate, the oxaloacetate is synthesized by cMDH. After import into glyoxysome, oxaloacetate is converted to citrate by CSY (Graham 2008; Pracharoenwattana and Smith 2008). The substrates and/or products of cytosolic ACO and MDH can be pulled away from the pathway of storage lipid conversion into sucrose and can be a fuel for other extraglyoxysomal processes (Fig. 1). Moreover, in Arabidopsis CSY catalyzes essential step in the respiration of fatty acids that bypass glyoxylate cycle, by translocating citrate into cytosol and channeling it into mitochondria (Pracharoenwattana et al. 2005).
Interplay between carbon and nitrogen metabolism is particularly important in legumes, whose seeds contain large amount of storage protein, but can accumulate large amount of lipid as well. For example, in yellow and Andean lupine seeds, the protein content reaches up to 35% of dry seed weight (Lehmann and Ratajczak 2008). Accumulation of storage lipid in lupine seeds is at a lower level. Yellow lupine seeds contain about 6%, white lupine 7–14% and Andean lupine about 20% oil of dry seed weight (Borek et al. 2009). Mature, air-dried lupine seeds do not contain starch (Borek et al. 2006; Duranti et al. 2008). In germinating lupine seeds, lipid-derived carbon skeletons are used as respiratory substrates and enter pathway of sugars synthesis; however, it was established in experiments with exogenously applied radiolabeled acetate that they are also used in amino acids synthesis (Fig. 1). The high incorporation of carbon atoms coming from storage lipid has been noticed especially in amino acids belonging to the primary amino acid biosynthesis pathway, i.e. asparagine as well as glutamine, glutamate and aspartate (Borek et al. 2003; Borek and Ratajczak 2010). It is postulated that the intermediates of glyoxylate cycle are the source of carbon skeletons for amino acids synthesis (Borek and Ratajczak 2010). In the course of storage protein mobilization during legume seeds germination, large quantities of ammonium are produced. Asparagine, as a temporary storage form of ammonium, is accumulated and in germinating lupine seeds it may reach up to 30% of seed dry weight (Lehmann and Ratajczak 2008). Ammonium reassimilation is catalyzed by collaborated action of glutamine synthetase (GS) and asparagine synthase (AS) (Lehmann and Ratajczak 2008) (Fig. 1).
In research experiments related to storage lipid catabolism in germinating seeds and young seedlings, exogenously applied acetate is routinely used as the simplest fatty acids. In Arabidopsis seedlings, acetate is used as respiratory substrate but some exogenously applied acetate is directed into glyoxysome where it is activated to acetyl-CoA, and through glyoxylate cycle converted to malate, citrate or succinate. In the next step, it is translocated into mitochondria in the form of four- or six-carbon organic acids (Eastmond et al. 2000; Hooks et al. 2004; Turner et al. 2005; Kunze et al. 2006; Hooks et al. 2007) (Fig. 1).
To assess the importance of key steps in the pathways of carbon flow from storage lipid to sugars and amino acids in germinating lupine seeds, we used acetate labeled with 14C as well. By applying NaF, we attempted to limit carbon flow through gluconeogenesis (Fig. 1), but NaF inhibits glycolysis as well. Both processes are inhibited at the enolase (phosphoenolpyruvate hydratase) stage (Miller 1993; Qin et al. 2006). We tested mixture of mitochondrial electron transport chain inhibitors (KCN, NaN3 and SHAM) known to suppress mitochondrial respiration, and MSO as a glutamine synthetase inhibitor which restricts amino acids synthesis (Masclaux-Daubresse et al. 2006) (Fig. 1). Moreover, we investigated the regulatory function of sucrose. We focused our studies on sucrose because besides serving as a respiratory substrate and carbon skeletons donor, sucrose is important regulatory compound which controls plant cell metabolism by modifying the expression of many genes (see reviews of Koch 1996; Rolland et al. 2006; Ramon et al. 2008) among them the genes encoding for the enzymes involved in lipid and protein catabolism. For example, sugar starvation enhances expression and activity of MLS and ICL (Graham et al. 1994) and glutamate dehydrogenase (GDH) (Lehmann et al. 2003, 2010). On the other hand, sugar supply results in a decrease or total block in storage lipid breakdown (Eastmond et al. 2000; To et al. 2002). Our experiments were conducted on in vitro grown embryo axes and cotyledons of three lupine species (yellow, white and Andean) which are significantly different in seed storage lipid content. The regulatory function of sucrose in storage compound mobilization was investigated at ultrastructure level and by investigating the metabolism of exogenously applied 14C-acetate. The results of our studies enabled us to determine how two major storage compounds are catabolized in germinating lupine seeds and to gain more insight into the interconnections between pathways of storage protein and storage lipid degradation. Our studies also provided an answer to our question at what degree the limited supply of main respiratory substrates, which are amino acids in germinating lupine seeds, can be compensated by enhanced storage lipid breakdown?
Materials and methods
Plant material and growth conditions
Seeds of yellow, white and Andean lupine were surface sterilized in 0.02% mercuric chloride for 10–15 min and allowed to imbibe for 24 h at 25°C in darkness. Embryo axes and cotyledons were excised from imbibed seeds and were grown in vitro for 96 h on Heller (1954) medium supplemented with 60 mM sucrose (+S) or without the sugar (−S). The in vitro culture was kept in darkness at 25°C.
Preparation of tissues for transmission electron microscopy (TEM)
Ultrastructure observations were made in meristematic zone of embryo axes and in parenchyma cells of the central part of cotyledons. Embryo axes and cotyledons of dry seeds, imbibed seeds (24 h), and seed organs grown in vitro for 96 h on medium with 60 mM saccharose (+S) or on medium without the sugar (−S) were used. Appropriate fragments of embryo axes and cotyledons were fixed in Carnoy’s half-strength fixative (Karnowsky 1965), i.e. in a mixture of 4% glutaraldehyde and 4% paraformaldehyde (1/1 v/v). Postfixation was conducted in 1% OsO4. The samples were stained in 2% aqueous solution of uranyl acetate. Dehydration was performed in a series of acetone solutions. The samples were embedded in epoxy resin of low viscosity (Spurr 1969). Ultrathin sections were prepared using Ultracut S Raichert, stained in 5% uranyl acetate and 0.5% lead citrate and observed under the transmission electron microscope TEM-1200 Ex JEOL.
Respiration of embryo axes and cotyledons
Assay of respiration of yellow lupine embryo axes and cotyledons was performed with a Clark electrode (Rank Bothers, Digital Model 10). The organs were incubated in Petri dishes in 8 ml of Heller medium (+S and −S) supplemented with NaF (inhibitor of glycolysis/gluconeogenesis) and mixture of KCN, NaN3 and SHAM (inhibitors of mitochondrial respiration). After 105 min of incubation, the embryo axes and cotyledons were transferred into a vessel of Clark electrode containing 3 ml of incubation media and the respiration was measured during the next 15 min. The following concentrations of the inhibitors were used: 30 mM NaF, 0.035 mM KCN, 0.035 mM NaN3 and 0.15 mM SHAM.
Experiments with radiolabeled acetate
Radiolabeled experiments were conducted according to modified method described previously (Borek et al. 2003). Embryo axes (8 samples) and cotyledons (8 samples of yellow and Andean lupine and 6 samples of white lupine) grown in vitro were incubated in 8 ml of Heller medium (+S and −S) containing 30 mM NaF (inhibitor of glycolysis/gluconeogenesis), mixture of 0.035 mM KCN, 0.035 mM NaN3, 0.15 mM SHAM (inhibitors of mitochondrial respiration) or 1 mM MSO (inhibitor of glutamine synthetase). Concentrations of NaF, KCN, NaN3 and SHAM were adjusted so that after 120 min of incubation of the organs, their respiration rate decreased by about 50% (Table 1). MSO concentration was determined upon literature data (Masclaux-Daubresse et al. 2006). Embryo axes and cotyledons were incubated in inhibitor solutions for 60 min and after this accommodation period, 925 kBq of 1- or 2-14C acetate was added into incubation media. Pieces of filter paper soaked with 200 μl of 20% KOH were placed above the surface of the incubation media. The pieces were removed in 15-min intervals, i.e. at 75, 90, 105 and 120 min of incubation period, and their radioactivity was measured using Packard Tri-Carb 2100 scintillation counter. Control incubations were performed since acetate is a volatile compound. 1- and 2-14C acetate (925 kBq) was added to 8 ml of distilled water to assess the level of spontaneous release of 14CO2. The radioactivity of 14CO2 released under control conditions was subtracted from the values measured in experimental variants with embryo axes and cotyledons. After 120 min of incubation, embryo axes and cotyledons were collected, washed in distilled water and transferred to 8 ml of 80% ethanol. After 2 weeks, the plant material was air-dried for 24 h. The radioactivity of ethanol-soluble and -insoluble fractions of organs was measured using Packard Tri-Carb 2100 scintillation counter.
Statistical analysis
Data presented in Figs. 5, 6, 7 and in Tables 1, 2, 3, 4 are means of three replications. SD was calculated and did not exceed 10%. In Figs. 2, 3, 4and in Tables 2, 3, 4, the SD values were omitted to clarify the presentation.
Results
Ultrastructure of embryo axes and cotyledons
In embryo axes and cotyledons of mature, air-dried seeds of yellow, white and Andean lupine, protein and oil were two major storage compounds detected, while no starch grains were visible (Figs. 2, 3, 4a, e). Storage protein was located nearly exclusively in cotyledons (Figs. 2, 3, 4e), whereas oil bodies occurred mostly in cotyledons, and occasionally in embryo axes (Figs. 2, 3, 4a, e). In embryo axes and cotyledons, oil bodies were located as a single or double layer near the plasmalemma (Figs. 2, 3, 4a, b). In cotyledons, oil bodies occurred near the plasmalemma and additionally were scattered throughout cytosol (Figs. 2, 3, 4e, f). Ultrastructure observations clearly showed differences in oil body number in seeds of the three investigated lupine species. The differences were visible both in embryo axes and in cotyledons of dry and imbibed seeds. The least oil bodies were visible in embryo axes and cotyledons of yellow lupine seeds (Fig. 2a, b, e, f). Clearly more oil bodies could be detected in organs of white lupine seeds (Fig. 3a, b, e, f), while the most oil bodies were observed in embryo axes and cotyledons of Andean lupine seeds (Fig. 4a, b, e, f). Starch appeared in embryo axes and cotyledons during imbibition of white lupine seeds. Numerous small or quite large starch grains were located in embryo axes (Fig. 3b), and the smaller ones occurred in cotyledons (Fig. 3f). However, starch grains did not occur in organs of imbibed seeds of yellow (Fig. 2b, f) and Andean lupine (Fig. 4b, f).
Apparent differences were noted in ultrastructure of embryo axes and cotyledons grown for 96 h in vitro on medium with (+S) and without (−S) sucrose. In yellow lupine embryo axes grown on medium +S oil bodies were completely degraded, small starch grains and very small amount of storage protein were visible (Fig. 2c). In sucrose starved (−S) yellow lupine embryo axes there were no oil bodies, while starch and protein level and additionally the vacuolization of cells was significantly increased (Fig. 2d). In white lupine embryo axes fed with sucrose (+S), few oil bodies and numerous large starch grains were visible (Fig. 3c). High vacuolization indicating advanced autophagy occurred in cells of sucrose starved (−S) embryo axes. Moreover, more oil bodies were visible, compared to organs fed with sucrose (Fig. 3d). In Andean lupine embryo axes grown on medium supplemented with sucrose (+S), there where clearly more oil bodies than in analogous embryo axes of white lupine. Small and not numerous starch grains and small deposits of storage protein were visible (Fig. 4c). In Andean lupine embryo axes grown on sucrose-free medium (−S), increased content of oil bodies and increased vacuolization was detected (Fig. 4d). In cotyledons grown in vitro, changes caused by culture trophy conditions were similar in all three lupine species: in cotyledons grown on medium without sucrose (−S), smaller and fewer starch grains, smaller amount of storage protein, and fewer oil bodies than in sucrose fed cotyledons, were observed (+S; Figs. 2, 3, 4g, h).
Respiration of embryo axes and cotyledons
The respiration of yellow lupine cotyledons was higher than that of embryo axes. The trophic conditions of in vitro culture (+S and −S) did not cause significant change in O2 consumption by cotyledons but respiration of sucrose starved (−S) embryo axes was considerably lower than respiration of embryo axes fed with sucrose (+S) (control in Table 1).
Radioactivity of CO2 produced from acetate by embryo axes and cotyledons
Small amounts of radioactive CO2 released by embryo axes and cotyledons from 1- and 2-14C acetate were detected after 15 min of incubation. From this stage, the level of CO2 radioactivity has been increasing exponentially to the end of experimental period, i.e. till 120 min (Figs. 5, 6, 7a–h). Remarkably higher release of radioactive CO2 was noted from 1-14C (Figs. 5, 6, 7a, c, e, g) than from 2-14C (Figs. 5, 6, 7b, d, f, h) acetate. It was most clearly visible in yellow and Andean lupine cotyledons where radioactivity of CO2 with carbon atom from C-1 position of acetate was almost 20-fold higher than radioactivity of CO2 with carbon atom from C-2 position of acetate (control on Fig. 5g, h and MSO on Fig. 7e, f, respectively). In all three lupine species, more radioactive CO2 (both from 1- and 2-14C acetate) was released by embryo axes (Figs. 5, 6, 7a–d) than cotyledons (Figs. 5, 6, 7e–h) and this liberation was clearly higher (without few exceptions) in organs grown on medium without sucrose (−S). It was the most evident in starved embryo (−S) axes of yellow and white lupine which produced 14CO2 from 2-14C acetate over sixfold more intensely than embryo axes fed with sucrose (+S) (controls on Figs. 5b, d, 6b, d, respectively). Among the inhibitors, NaF caused the highest suppression in liberation of 14CO2 (both from 1- and 2-14C acetate) in all studied organs (Figs. 5, 6, 7a–h). The mixture of KCN, NaN3 and SHAM caused inhibition of 14CO2 production as well, but to a lesser extend than NaF (Figs. 5, 6, 7a–h); in two cases a stimulatory effect on 14CO2 liberation was observed (liberation of CO2 with C-2 of acetate by white lupine embryo axes and cotyledons grown on +S medium; Fig. 6b, f). MSO stimulated liberation of 14CO2 in embryo axes and cotyledons of yellow lupine and white lupine grown on medium with sucrose (+S) (Figs. 5a, b, e, f, 6a, b, e, f, respectively). In Andean lupine, MSO caused higher production 14CO2 compared to the control both in organs grown on medium with (+S) and without sucrose (−S) (Fig. 7a–h).
Radioactivity of ethanol fractions of embryo axes and cotyledons
Generalizations are necessary when describing the radioactivity of ethanol-soluble and -insoluble fractions. In all three investigated species, considerably more radioactivity was found in ethanol fractions than in the liberated 14CO2 (Tables 2, 3, 4). Ethanol-soluble fraction was more radioactive than the -insoluble one. Embryo axes accumulated radioactivity with higher efficiency than cotyledons (Table 2, 3, 4). The difference was the highest in sucrose-fed (+S) embryo axes of white lupine that accumulated over threefold more radioactivity than cotyledons (the sum of ethanol-soluble and -insoluble fractions of +S controls in Table 3). The lack of sucrose in medium (−S) caused a decrease in the radioactivity of ethanol-soluble and -insoluble fractions of yellow (with one exception) and white lupine embryo axes and cotyledons (compare +S and −S controls in Tables 2, 3), whereas in Andean lupine it caused an increase in the fractions’ radioactivity (Table 4). The accumulation of carbon atoms from C-1 and C-2 positions of acetate in embryo axes and cotyledons was at a similar level. The respiratory inhibitors, with few exceptions, caused the same effect on the radioactivity of ethanol-soluble and -insoluble fractions, like on 14CO2 liberation, i.e. the radioactivity of ethanol fractions was strongly decreased by NaF and to a lesser degree by the mixture of KCN, NaN3 and SHAM (Tables 2, 3, 4). The changes in radioactivity of ethanol fractions caused by MSO were similar to those observed in 14CO2 liberation by embryo axes and cotyledons of only yellow and Andean lupine. In yellow lupine, MSO caused an increase in radioactivity in organs fed with sucrose (+S) and decrease in organs grown on −S medium (Table 2). In Andean lupine, MSO caused an increase of ethanol fractions radioactivity of both organs grown on +S and −S media (Table 4). Larger and ambiguous divergences were observed in MSO influence on radioactivity accumulation in white lupine embryo axes and cotyledons. In this species MSO decreased radioactivity coming from 1-14C acetate in fractions of organs grown on +S and −S media (Table 3).
Discussion
The results presented in this paper show that sugar level in tissues controls the intensity of storage compound breakdown during germination of protein-storing lupine seeds. In lupine embryo axes and cotyledons grown in vitro on medium without sucrose (−S), a decreased concentration of soluble sugars and lower content of temporary starch is observed (Borek et al. 2006). Decreasing of sugar concentration in tissues causes changes in the metabolism that allow supplying of respiratory substrates. A proof for enhancement of storage compound degradation in sugar depletion conditions are changes observed in ultrastructure of embryo axes and cotyledons of all three investigated lupine species. Changes visible in ultrastructure of cotyledons were unambiguous and dependent on decreasing of storage protein content, oil content, and frequency and size of starch grains (Figs. 2g, h–4g, h). In cells of root meristematic zone of sucrose starved (−S) embryo axes, an increased vacuolization level was observed despite of diminishing or disappearing of storage protein and starch (Figs. 2c, d, 3, 4c, d). This indicates on advanced autophagy (Marty 1999), which is a mechanism for degradation of cellular contents in order to recycle nutrients, especially protein (Thompson and Vierstra 2005). In plants, autophagy has been known to be important for nutrient remobilization during sugar and/or nitrogen starvation (Thompson and Vierstra 2005; Lundgren Rose et al. 2006; Bassham 2007). In sucrose starved (−S) white and Andean lupine embryo axes, numerous oil bodies were noticed (Figs. 3c,d, 4c, d). This observation is surprising and difficult to interpret. However, as autophagy causes massive and non-specific degradation of plasma membranes (Aubert et al. 1996), we hypothesize that the second source (besides lipid stored in oil bodies) of fatty acids appears in cells. Moreover, in such conditions, the synthesis of the new compounds of plasma membranes is restricted. Contrary to this, in conditions of good supply of tissues in sugars (+S), more intensive growth of white and Andean lupine embryo axes occurs (data not presented), so oil bodies are degraded faster. Because of that, in sucrose fed (+S) white and Andean lupine embryo axes there are less numerous oil bodies than in sucrose starved (−S) ones. Additionally, since one of the main aims of autophagy during starvation is the supply of respiratory substrates (which in germinating lupine seeds are amino acids), this process should be induced by amino acids deficit. It is possible that vacuolization, as a result of autophagy, could be visible in cells even before total degradation of oil bodies. This was observed in white and Andean lupine embryo axes, because seeds of those species contain relatively a lot of storage lipid (7–14 and about 20%, respectively) (Borek et al. 2009). Yellow lupine seeds contain less storage lipid (about 6%) (Borek et al. 2009), so it was completely mobilized before the non-specific, autophagic degradation occurred. The higher content of oil bodies in sucrose starved (−S) embryo axes corresponds with the analytical measurements of the total lipid content in whole lupine organs grown in vitro. They show the higher total lipid content in starved (−S) than in sucrose fed (+S) embryo axes (data not presented). Autophagic processes were not visible in cells of cotyledons, because the lack of sucrose in the culture medium did not generate sugar starvation in these organs during 96 h.
Exogenously applied acetate was taken up by embryo axes and cotyledons and accumulated in large quantities in organ fractions (controls in Tables 2, 3, 4). However, some of it was released as 14CO2 (Figs. 5, 6, 7), which suggests that acetate was used as a respiratory substrate as well (after its earlier activation to acetyl-CoA) (Fig. 1). The radioactivity accumulated in organ fractions was considerably higher than that of CO2 (compare controls in Tables 2, 3, 4 and controls on Figs. 5, 6, 7). Release of 14CO2 from exogenous acetate by organs treated with respiratory inhibitors did not correlate with O2 uptake. Respiratory inhibitors decreased respiration to about 50% (Table 1), but NaF and the inhibitors of mitochondrial electron transport chain (KCN, NaN3 and SHAM) retarded oxidation of acetate considerably stronger than O2 consumption. The effect of NaF was stronger than that of the inhibitors of mitochondrial electron transport chain (Figs. 5, 6, 7). Such intensive inhibitory effect of NaF on 14CO2 liberation (as well as on radioactivity of ethanol-soluble and -insoluble fractions; Tables 2, 3, 4) is probably a result of non-specific action of NaF. Enolase is inhibited by direct binding of fluoride to the enzyme (Qin et al. 2006). However, fluoride is considered as an agent that binds Ca+2 and Mg+2, and in that way decreases availability of these ions to many enzymes. In this way NaF may cause disruption in many processes. This second mechanism of inhibitory effect of NaF was proposed, for example, for H+-ATPase (Reddy and Kaur 2008).
The use of acetate as a respiratory substrate, was increased in organs grown on medium without sucrose (−S). These organs produced more 14CO2 (Figs. 5, 6, 7) and lower radioactivity of ethanol-soluble and -insoluble fractions was observed (controls in Tables 2, 3, 4). It is consistent with many literature data describing enhancement in respiring other substrates than glucose in sugar deficiency conditions (Koch 1996; Yu 1999; Devaux et al. 2003; Rolland et al. 2006; Ramon et al. 2008). Moreover, the carbon atom in radioactive CO2 originated mostly from the C-1 position of acetate. This result indicates that TCA cycle is not the sole source of radioactive CO2. One of biochemical processes producing CO2 is the conversion of isocitrate into 2-oxoglutarate catalyzed by cytosolic NADH+-dependent isocitrate dehydrogenase (IDH) (Fig. 1). Next, 2-oxoglutarate undergoes transamination and takes part in GS/GOGAT cycle giving glutamate (Bewley and Black 1994). The activity of NADH+-IDH was proved previously in germinating yellow lupine seeds (Borek et al. 2006) and the enzyme is very important in carbon flow from fatty acids to amino acids in germinating yellow lupine seeds (Borek and Ratajczak 2010). Other source of 14CO2 may be phosphoenolpyruvate carboxykinase (PEPCK) (Fig. 1). This enzyme takes part in gluconeogenesis and its activity in germinating yellow lupine seeds has also been detected (Ratajczak et al. 1998). However, our earlier results on yellow lupine seed germination with exogenously applied 1- and 2-14C acetate have shown lower flow of radioactivity into sugars than into amino acids (Borek et al. 2003; Borek and Ratajczak 2010), which suggests restricted carbon flow through PEPCK. Therefore, the release of 14CO2 with the carbon atoms from C-2 position may be attributed only to reactions of the TCA cycle. Higher incorporation of carbon atom from position C-1 than C-2 of exogenous acetate into CO2 has been previously observed in seedlings of yellow lupine and this phenomenon has been analyzed in details in our earlier publication (Borek et al. 2003).
Lupine seeds accumulate high amount of protein as a main storage compound. For example, in yellow and Andean lupine seeds protein content can reach up to 35% of dry weight (Lehmann and Ratajczak 2008). Temporary increase of free amino acids as a result of storage protein catabolism during germination is observed and glutamate occupies the central position in complex amino acid metabolism. It is a precursor of many compounds and is transaminated with many amino acids (Bewley and Black 1994). In the course of storage protein mobilization and amino acid metabolism large quantities of ammonium are produced. As nitrogen in this form is toxic (Britto and Kronzucker 2002), its utilization is necessary. Asparagine as a temporary storage form of ammonium is accumulated (up to 30% of dry weight) (Lehmann and Ratajczak 2008). Ammonium is reasimilated in two steps. First, glutamine is synthesized from glutamate and ammonium by GS and next, through the action of AS, it is stored in asparagine (Lehmann and Ratajczak 2008) (Fig. 1). A source of carbon skeletons for such intensive asparagine synthesis is necessary. Other amino acids can be one of such source, but during germination and young seedling development most of amino acids are used for protein synthesis in developing embryo axes and some of amino acids are respired (Ratajczak et al. 1996; Borek et al. 2001; Lehmann and Ratajczak 2008) (Fig. 1). Carbon skeletons for asparagine synthesis do not come from carbohydrates, because mature, air-dried yellow, white and Andean lupine seeds do not contain starch as a storage compound (Figs. 2, 3, 4a, e; Borek et al. 2006; Duranti et al. 2008). In such circumstances, fatty acids coming from storage lipid breakdown may be a source of carbon skeletons for asparagine synthesis (Fig. 1). This possibility was proved in germinating yellow lupine seeds (Borek et al. 2003; Borek and Ratajczak 2010). Several alternative pathways leading from storage lipid to asparagine are proposed. Citrate, isocitrate, malate and oxaloacetate synthesized in glyoxylate cycle are the main intermediates directed to amino acids synthesis (Fig. 1). It may be direct synthesis of amino acids from isocitrate (through 2-oxoglutarate) or oxaloacetate, or after their previous conversion into malate and 2-oxoglutarate in TCA cycle (Borek and Ratajczak 2010). The results presented here indirectly confirm carbon flow from storage lipid into amino acids in germinating lupine seeds. GS inhibitor caused an increase in CO2 radioactivity (Figs. 5, 6, 7) and radioactivity of ethanol-soluble and -insoluble fractions of embryo axes and cotyledons (Tables 2, 3, 4). This was most evident in embryo axes and cotyledons of Andean lupine, i.e. species which seeds contain the highest (among the lupine species studied) amount of storage lipid (about 20% of dry weight) (Borek et al. 2009). Increase in CO2 and fractions radioactivity caused by MSO was observed in all experimental variants (Fig. 7a–h; Table 4). Inhibition of GS causes disruption in asparagine synthesis and simultaneous disruption in amino acids oxidation. Physiological meaning of the enhancement of lipid catabolism is obvious. A block in oxidation of main respiratory substrate, which in germinating lupine seeds are amino acids coming from storage proteins, causes an increase in catabolism of other potential respiratory substrates, i.e. fatty acids. The mechanism of this phenomenon is not known in details and needs additional research. However, our results suggest that one of the regulatory agents is the sucrose level in tissues of studied organs. Oxidation of labelled acetate was considerably higher in organs grown on −S medium. It was the clearest visible in 2-14C acetate catabolism by sucrose starved embryo axes (Figs. 5, 6, 7); however, MSO caused simultaneous increase in radioactivity of ethanol-soluble and -insoluble fractions (Tables 2, 3, 4). So, lipid is not only a respiratory substrate, but it is also used in non-respiratory processes (Fig. 1). Using exogenous 14C-acetate, it has been proved previously that in germinating yellow lupine seeds lipid-derived carbon is directed more efficiently into amino acids than into sugars (Borek et al. 2003; Borek and Ratajczak 2010). Such tendency is opposite to that observed in germinated oil-storing seeds, in which storage lipid is used as respiratory substrate and is converted mainly into sugars (Beevers 1976, 1979; Eastmond and Graham 2001; Penfield et al. 2005, 2006; Graham 2008; Quettier and Eastmond 2009; Kaur et al. 2009). The data presented in this paper indicate peculiar features of lipid catabolism in germinating lupine seeds as a result of high storage protein level.
The data presented here are based on our comparative study of storage lipid breakdown and interconnections between storage lipid and protein catabolism during seed germination of three species of the lupine genus. They clearly indicate that different strategies of storage compound mobilization occur during germination depending on proportions of lipid and protein content in lupine seeds. In germinating yellow lupine seeds, which have the highest storage protein to lipid ratio (Fig. 2e–h; Lehmann and Ratajczak 2008; Borek et al. 2009), metabolism dependents on amino acids. In germinating seeds of this lupine species, amino acids are used not only for protein synthesis but are respired, too. Disruption in amino acids metabolism (MSO treatment) cannot be efficiently compensated by enhancement of breakdown of spare amount of storage lipid. Such situation is enhanced additionally in sugars deficiency conditions in tissues (Fig. 5c, d, g, h; Table 2). In white lupine seeds, storage protein dominates as well, but storage lipid content is higher than in yellow lupine seeds (Fig. 3e, f; Borek et al. 2009). During white lupine seeds germination, the efficient synthesis of temporary starch takes place (already during imbibition) (Fig. 2b, f). So, in germinating white lupine seeds, the very convenient storage compound appears and can be used for respiration and for anabolic processes. In such circumstances, de-organization of amino acid metabolism does not cause the quick increase in storage lipid breakdown (Fig. 6a–h; Table 3). Opposite to the two strategies of storage compounds breakdown described above, the third one is possible in germinating Andean lupine seeds. The seeds of this lupine species accumulate high amount of storage protein, but contain high amount of storage lipid as well (Fig. 4e–h; Lehmann and Ratajczak 2008; Borek et al. 2009). Because of that, in germinating Andean lupine seeds an efficient pathway of storage lipid catabolism has to occur. In the case of blocking of storage protein catabolism, the storage lipid breakdown can efficiently compensate amino acids supply and fatty acids can be used both for respiration and for anabolic processes. This strategy can function even in conditions of sucrose deficiency in tissues (Fig. 7a–h; Table 4).
Abbreviations
- ACO:
-
Aconitase
- AS:
-
Asparagine synthase (glutamine-hydrolysing)
- AT:
-
Aminotransferases
- cMDH:
-
Cytosolic fraction of malate dehydrogenase
- CSY:
-
Citrate synthase
- GDH:
-
Glutamate dehydrogenase
- gMDH:
-
Glyoxysomal fraction of malate dehydrogenase
- GOGAT:
-
Glutamate synthase
- GS:
-
Glutamine synthetase (glutamate-ammonia ligase)
- ICL:
-
Isocitrate lyase
- IDH:
-
Isocitrate dehydrogenase
- KCN:
-
Potassium cyanide
- MSO:
-
l-methionine sulfoximine
- NaF:
-
Sodium fluoride
- MDH:
-
Malate dehydrogenase
- MLS:
-
Malate synthase
- NaN3 :
-
Sodium azide
- PEPCK:
-
Phosphoenolpyruvate carboxykinase
- SHAM:
-
Salicylhydroxamic acid
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This work was supported by grant no. 2 P06A 004 29 from Polish science funding in years 2005–2008.
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Borek, S., Kubala, S., Kubala, S. et al. Comparative study of storage compound breakdown in germinating seeds of three lupine species. Acta Physiol Plant 33, 1953–1968 (2011). https://doi.org/10.1007/s11738-011-0744-6
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DOI: https://doi.org/10.1007/s11738-011-0744-6