Abstract
Purpose
Tropical forests exchange large amounts of greenhouse gases (GHGs: carbon dioxide, CO2; methane, CH4; and nitrous oxide, N2O) with the atmosphere. Forest soils and stems can be either sources or sinks for CH4 and N2O, but little is known about what determines the sign and magnitude of these fluxes. Here, we aimed to study how stem and soil GHG fluxes vary along a topographic gradient in a tropical forest.
Methods
Fluxes of GHG from 56 individual tree stems and adjacent soils were measured with manual static chambers. The topographic gradient was characterized by a soil moisture gradient, with one end in a wetland area (“seasonally flooded”; SF), the other end in an upland area (“terra firme”; TF) and in between a transitional area on the slope (SL).
Results
Tree stems and soils were always sources of CO2 with higher fluxes in SF compared to TF and SL. Fluxes of CH4 and N2O were more variable, even within one habitat. Results showed that, in TF, soils acted as sinks for N2O whereas, in SF and SL, they acted as sources. In contrast, tree stems which were predominantly sources of N2O in SF and TF, were sinks in SL. In the soil, N2O fluxes were significantly influenced by both temperature and soil water content, whereas CH4 fluxes were only significantly correlated with soil water content.
Conclusion
SF areas were major sources of the three gases, whereas SL and TF soils and tree stems acted as either sources or sinks for CH4 and N2O. Our results indicate that tree stems represent overlooked sources of CH4 and N2O in tropical forests that need to be further studied to refine GHG budgets.
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Introduction
Tropical forests are a major component of the global carbon cycle (Mitchard 2018), mainly because they store half the world’s forest biomass carbon (Pan et al. 2011) and represent about half of the global terrestrial carbon sink, taking up about 15% of the anthropogenic carbon emissions annually (Phillips and Brienen 2017). As part of the climate system, ecosystem uptake or emissions of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) can mitigate or exacerbate global warming (Butterbach-Bahl et al. 2004). Fluxes of these greenhouse gases (GHGs) naturally occur in tropical forests but their quantification, origins and environmental controls still need to be determined. Studying soil and stem GHG fluxes along natural topographic transects is relevant because these transects cover large gradients in soil texture, water content and nutrient availability (Van Langenhove et al. 2021) and also exhibit differences in standing biomass and tree productivity (Ferry et al. 2010). This large range of soil properties along a topographic gradient is likely to influence GHG fluxes from soils and stems.
Soil CO2 fluxes, resulting from both root and microbial activity, can be affected either directly by soil temperature, water content, nutrients and dissolved organic matter (Fang et al. 2009; Whitaker et al. 2014; Auffret et al. 2016), or indirectly by changes in soil texture and vegetation (Luizão et al. 2004; Epron et al. 2006; Bréchet et al. 2009), and hence vary with topographic position. High soil water content under warm temperatures can stimulate soil CO2 efflux (Sorz and Hietz 2006; Barba et al. 2016), and, by promoting sap flux and stem respiration, also increase stem CO2 efflux (Ceschia et al. 2002).
In stems, CO2 may indeed be produced locally during the respiration required to sustain production of new woody tissues (i.e. growth respiration) and maintenance of living biomass (i.e. maintenance respiration; Ryan 1990; Maier 2001). The latter often explains the differences in respiration rate between small and large trees (Ryan and Waring 1992), as well as the size-related changes in the efficiency of stem carbon accumulation. Stem CO2 fluxes depend on tree height (Cavaleri et al. 2006; Katayama et al. 2014, 2016), season (Stahl et al. 2011) and elevation (Robertson et al. 2010), but not on bark thickness (Paine et al. 2010). In addition to the locally produced CO2, stem CO2 fluxes can also originate from respiration in the soil, where CO2 dissolved in water can be taken up by the roots and transported with the xylem sap through the stem (Saveyn et al. 2008; Teskey et al. 2008; Trumbore et al. 2013; Hilman and Angert 2016; Aubrey and Teskey 2021). A fraction of this CO2 can be fixed by photosynthetic cells in the wood or leaves (Teskey et al. 2008), whereas the rest will be emitted to the atmosphere and contribute to stem, branch and leaf CO2 fluxes. The CO2 emitted from stems thus originates from CO2 produced in both the woody tissue and soil (Teskey et al. 2017). Soil water extremes, such as flooding or drought, can reduce stem CO2 fluxes because they tend to reduce aerobic respiratory activity in soils (Stahl et al. 2011). Both stem and soil CO2 fluxes show seasonal patterns explained by interactions between temperature, soil water content and sap flow (e.g. Barba et al. 2019). Soil water content can inhibit the transverse transport of CO2 in trees, which correspond to the movement of CO2 from leaves to stem and roots for use in cellular respiration and other metabolic processes. Water is essential for the vertical movement of dissolved nutrients and gases in trees, including CO2. When soil water content is low, the water potential gradient between the soil and the roots decreases, making it more difficult for water and dissolved gases to move from the roots to the leaves (Sancho-Knapik et al. 2022). This can lead to a reduction in photosynthesis and transpiration, which can in turn reduce the CO2 emissions from the stems (Zhao et al. 2018).
Topography is characterized by a hydrological and nutrient gradient (from well-drained upland areas (“terra firme”; TF) with aerobic conditions to waterlogged wetland areas (“seasonally flooded”; SF) with anaerobic conditions (Ferry et al. 2010; Courtois et al. 2018). To gain more insight in the variation of CO2 fluxes across a tropical forest, the impact of topographic position on soil and stem CO2 fluxes needs to be studied.
Methane can both be emitted and taken-up by soils and stems. Soil CH4 uptake dominates in aerobic soils, such as the upland TF areas in tropical forests, and is generally a minor component of the forest GHG balance. Nonetheless, CH4 uptake is an important flux in the global budget of atmospheric CH4 since global aerobic soil surface is large (Dutaur and Verchot 2007; Saunois et al. 2020). In contrast, anaerobic soils, such as the SF areas in tropical forests, mainly emit CH4, because methanogenesis dominates over aerobic microbial methanotrophy.
Recent studies have demonstrated that also tree stems can be a source of CH4 (Pangala et al. 2013, 2017; Barba et al. 2019; Covey and Megonigal 2019; Epron et al. 2022). Tree stem CH4 emissions are currently unaccounted for as an emission compartment in the current global CH4 budget (Carmichael et al. 2014; Saunois et al. 2020). Moreover, several recent studies suggest that tree stem CH4 fluxes may occur across a range of ecosystems including mangroves (Jeffrey et al. 2019, 2020), wetland forests (Pangala et al. 2017; Terazawa et al. 2015; Sjögersten et al. 2020; Gauci et al. 2022), while even upland forests may emit CH4 (Covey et al. 2012; Machacova et al. 2016; Barba et al. 2019; Bréchet et al. 2021). These studies have demonstrated that tree stems can emit CH4 even if they grow on soils that consume CH4, and also that the drivers of spatial patterns and magnitudes of these fluxes remain poorly understood. Gauci et al. (2022) pinpointed a clear positive effect of water table on flooded-tree CH4 emissions. In trees, a large fraction of the emitted CH4 originates from CH4 production in anaerobic soil layers, where CH4 production exceeds CH4 consumption (Welch et al. 2019; Feng et al. 2022). The gas dissolved in the soil water is taken up and transported by the roots, thereby bypassing the soil’s uppermost aerobic layer where methanotrophy dominates (Megonigal and Guenther 2008). In addition to CH4 delivered by the xylem stream, CH4 can moreover be produced in the woody tissues by methanogenic archaeal communities decomposing the heartwood of trees (Yip et al. 2019). Low oxygen concentrations in woody tissues can create a suitable environment for methanogenic communities, enhancing their activity and abundance. Along a topographic gradient, trees in a specific local environment (TF or SF) can have specific water and oxygen contents, as well as specific methanogenic archaeal communities. By extension, it can be assumed that the origin and the amount of CH4 emissions in stems are species-specific.
As for CO2, in temperate forest the seasonal pattern in stem CH4 fluxes has been explained by temperature, soil water content and tree sap flow (Maier et al. 2018; Barba et al. 2019; Welch et al. 2019; Machacova et al. 2021). An increase in stem CH4 emissions can be correlated to an increase in soil and air temperature (Wang et al. 2016; Pitz et al. 2018; Barba et al. 2019), an increase in soil water content (Barba et al. 2019; Welch et al. 2019), or a decrease in water table depth (Pitz et al. 2018). In our study, we will examine the relationships between CH4 fluxes in stems and soil along a topographic gradient associated with different habitats and microenvironment conditions (Pitz et al. 2018; Barba et al. 2019). In order to understand the processes involved in the emission and consumption of CH4 in forest ecosystems, it is necessary to study the woody tissue biogeochemistry and anatomy and tree physiology (Covey and Megonigal 2019). Stem CH4 emissions are indeed correlated with physiological or anatomical and morphological properties of tree species (Wang et al. 2016; Warner et al. 2017; Sjögersten et al. 2020), such as wood density (Wang et al. 2016), wood structure (Sjögersten et al. 2020), tree diameter (Pitz et al. 2018) and sap flow rate (Barba et al. 2019; Pitz and Megonigal 2017).
In soils, N2O is naturally produced in a wide range of nitrogen turnover processes, mainly by nitrification and denitrification processes (Davidson et al. 2007). Nitrification is an oxidative process, dominating in aerated soils. In aerobic soils, such as the TF areas in forests, consumption of N2O typically exceeds production of N2O, thereby exhibiting lower N2O emissions and even N2O uptake. However, nitrate leaching into lower anaerobic soil layers may be denitrified, causing N2O production and emission. Under the same anaerobic conditions where methanogenesis dominates, denitrification indeed dominates (Davidson et al. 2000). Denitrification by many bacterial and fungal taxa not only produces N2O; under anoxic conditions, N2O can be further reduced to N2, thus yielding lower N2O emissions (Smith et al. 2003). In ecosystems exhibiting variation in soil water, spatial and temporal variation in N2O emissions and uptake is thus extremely high, depending on variation in nitrate production and in N2O production and consumption. As the water-filled pore space decreases and the concentration of oxygen rises, the aerobic metabolism of bacteria, archaea and fungi can outcompete the anaerobic metabolism, lowering the rate of N2O emission and increasing the probability for net N2O emissions.
In trees, N2O dissolved in soil water can be absorbed by the roots and transported with the transpiration stream (Machacova et al. 2013). The role of trees in forest N2O budgets has been largely overlooked (but see: Machacova et al. 2016; Wen et al. 2017; Welch et al. 2019). Studies on mature trees growing in natural field conditions are limited and have revealed notable N2O emissions from stems (Díaz-Pinés et al. 2016; Machacova et al. 2016). In boreal forest, a study revealed that stem N2O fluxes can be linked to the tree’s physiological activity, such as gross primary productivity and evapotranspiration (Machacova et al. 2019). In temperate forest, stem N2O emissions in upland trees occurred even without aerenchyma (a specific plant tissue facilitating gas exchange along stems), and were associated with the rates of xylem water transport (Díaz-Pinés et al. 2016). Stem N2O emissions might be a pathway of N2O produced in the soil and emitted from terrestrial ecosystems into the atmosphere. As for stem CH4 fluxes, an increase in stem N2O fluxes is expected with an increase in soil water content along a topographic gradient.
The simultaneous study of fluxes of these three GHGs from or into soils and stems may yield new insights on the complexity of forest ecosystems as sources and sinks of GHGs. The overall goal of this study was to characterize the spatial variation of CO2, CH4 and N2O fluxes and, more specifically, examine the effect of topography-driven variation in abiotic conditions on these fluxes in a tropical forest, in French Guiana. We hypothesized that 1) GHG fluxes measured on tree stems across a topographic transect show similar trends to those on soils, 2) abiotic factors such as soil temperature and soil water content that are known to control CO2, CH4 and N2O fluxes in soil, also drive fluxes in tree stems, and 3) tree properties that determine the conductivity of the GHGs, such as bark and sapwood density or bark thickness, co-determine the GHG fluxes from stems.
Materials and methods
Study site
The experiment was conducted at the Paracou research station (5°50’N, 52°55’W), located in the coastal area of French Guiana, South America. Paracou is a pristine tropical forest with an average tree density of 620 trees ha−1 and a tree species richness between 150 and 200 species ha−1, both for trees with diameter at breast height (1.30 m; DBH) > 10 cm. The Lecythidaceae, Fabaceae, Sapotaceae and Chrysobalanaceae families are the dominant plant families in this highly diverse forest (Gourlet-Fleury et al. 2004). The study site is characterized by a patchwork of hills (10 - 40 m a.s.l.) and soils are mostly nutrient-poor Acrisols (FAO / ISRIC / ISSS 1998) with pockets of sandy Ultisols. Soils developed over a Precambrian metamorphic formation, called the “Bonidoro series”, are composed of schist and sandstone with veins of pegmatite, aplite, and quartz (Bonal et al. 2008). Annual rainfall at the study site (2004 - 2015) averages 3100 ± 70 mm year−1 and mean annual air temperature is about 25.7 ± 0.1 °C (Aguilos et al. 2019). The north-south movement of the intertropical convergence zone strongly influences the precipitation regime and makes the tropical climate very seasonal. The wet season can last eight months (December - July) and alternates with a dry period of about four months (August - November) during which rainfall is generally less than 100 mm month−1.
Sampling design
The campaign was carried out in February 2020, i.e. during the wet season. The selection of the trees was based on a precise representation of the distribution of diameter classes in the experimental plots (Supplementary, Fig. S6). The experimental plots were in the footprint of the Guyaflux tower (Bonal et al. 2008). We selected three topographic positions along the topographic transect: 1) terra firme located on top of hills (TF), 2) slopes at intermediate elevation (SL) and 3) seasonally flooded at low elevation very close to the water of the permanent river (SF). These different topographic positions were characterized by differences in volumetric soil water content (mean values measured during the campaign: 0.17 ± 0.02 m3 m−3 in TF, 0.23 ± 0.02 m3 m−3 in SL and 0.46 ± 0.14 m3 m−3 in SF), but also in a suite of other environmental characteristics (Table 1). In this study, TF was present at the highest elevation level and its soils were typically characterized by a high clay content, water drainage, and organic matter content but a low pH. SF occurred at the lowest elevation and had soils with high water contents and bulk density but low root biomass and carbon content, likely due to the lower clay content (Soong et al. 2020). These soils experienced at least three consecutive months of flooding during the year (usually during the major rainy season between April and July; Ferry et al. 2010). Between TF and SF, SL was characterized by transitional soils (Table 1). Soil types were hypoferralic acrisol on TF, haplic acrisol on SL, and haplic gleysol on SF according to Epron et al. (2006). Briefly, in Epron et al. (2006), six soil cores (3.3-cm diameter, 6.0-cm depth) were sampled for each topographic transect. Root fragments (< 5-mm diameter) were washed, oven-dried at 60 °C to constant mass and weighed. Soil pH was determined in a 1:2.5 soil:water ratio. The six soil subsamples of each plot were pooled into a composite sample. The concentrations of organic carbon were determined on these 30 composite samples with a total organic carbon analyser (TOC-5050- Shimadzu, Japan).
Forty-five polyvinylchloride (PVC) collars of 20 cm in diameter were inserted into the soil one year prior to the first measurement (December 2018) to an average depth of 3.0 cm (± 0.5 cm). For each topographic position, five circular plots of 5 m radius were identified by three PVC collars arranged 1 m apart to form a triangle (Fig. 1). The diameter measurements and botanical determination of the trees maximum 5 m around the collars were carried out during the same period. A total of 56 trees were selected (ranging from 2 to 7 trees per plot), given in total 20 trees in TF and 18 in both SL and SF.
Soil and stem fluxes
Gas samples were taken with manual static chambers and a syringe. We took gas samples once per individual tree, between February 2 and 4, 2020. For the soil CO2, CH4 and N2O flux measurements, we used the PVC chambers described in Courtois et al. (2018) between 9 am and 3 pm to avoid diurnal variability (Bréchet et al. 2011; Courtois et al. 2018; Pavelka et al. 2018). Soil chambers‘volume and surface area were 2600 cm3 and 290 cm2, respectively. The dimensions of tree stem chambers were 10.0 cm length, 8.0 cm width, 10.5 cm depth and 84.0 cm2 surface area accounting for a total volume of 840 cm3. Manual tree stem chambers were made with Tupperware boxes (LocknLock, Seoul, Korea), allowing us to fix them on all trees bigger than 12 cm diameter with straps and rubber Teroson (Henkel, Dusseldorf, Germany). We installed the stem chambers at 1.30 m above the soil surface. In total, 180 soil and 280 measurements of GHG concentration in the tree stems were made during the field campaign. Four gas concentration measurements per collar and per tree were taken to compute the GHG fluxes. We performed a single measure for each selected tree in the three habitats during the wet season. During the sampling period, SF was indeed flooded with high soil water content (0.46 m3 m−3) in our wet season of measurements. Gas samples were extracted from chambers at 0, 10, 20 and 30 min for soil and tree stems. In the soil and stems, air samples were taken with a 15-mL syringe whose needle was inserted through a septum in the chamber and then injected into pre-evacuated 12-mL vials (Labco Limited, Ceredigion, UK). The chambers were not ventilated and after the first air sampling, the air inside the chamber headspace was mixed five times with the syringe prior sampling. For each sample, concentrations of CO2, CH4 and N2O were determined by gas chromatography (Trace GC Ultra, Thermo Fisher Scientific, Vienna, Austria) and a vacuum dosing system (S + H Analytics, Germany) at 50 °C on a molecular sieve column (ShinCarbon ST 100 / 120, 2 m × 1 mm ID 1 / 16” OD, Restek). We used a flame ionization detector (FID) with a methanizer for CH4 detection and a pulsed-discharge detector for N2O detection. Calculation of minimum detectable flux (MDF) of CH4 and N2O was made with the methodology developed by Parkin et al. (2012). At sampling time 0, the mean concentration (that is, ambient concentration) were, for N2O, 0.360 ppm and 0.380 ppm for the soil and stem, respectively, and for CH4, 2.17 ppm and 2.22 ppm for the soil and stem, respectively. The soil CH4 and N2O MDF was 9.80 μgC m−2 h−1 and 13.06 μgN m−2 h−1, implying that values of CH4 fluxes within the range [−9.80; +9.80 μgN m−2 h−1] were included in the analysis as null fluxes (idem for N2O; Table S2 in Supplementary).
In addition, flux determination using manual chamber techniques in the soil and stems relied on discrete samples collected from a chamber headspace over fixed time intervals at 0, 10, 20 and 30 min. Flux computation were determined as the change in gas concentration over the time using linear or exponential curve fitting procedures.
Fluxes were computed with the “gasfluxes” package (version 0.4 - 4; Fuss 2020) for the three gases using linear regression (LR), or revised Hutchinson / Mosier (HMR) methods following recommendations from Pedersen et al. (2010) where HMR fluxes with the modified H / M technique from gas concentrations of each time interval (C0, C1, C2, and C3) as:
where fo is the calculated flux, C0 is the headspace concentration at time 0, CA1,2 is the average of the headspace concentrations at time C1 and C2, and C3 is the chamber headspace gas concentration at time 3. The term “tA1,2” is the time interval corresponding to the average of time 1 and time 2 (or one half of the total chamber deployment time). Gasfluxes provides functions for fitting non-linear concentration time models as well as convenience functions for checking data and combining different calculation methods. HMR is robust against horizontal gas transport and patterns of non-linearity, which reduces several constraints on static chamber methods, such as insertion depth and deployment time.
After flux computations, 62% of CO2 fluxes were fitted with HMR methods and 38% with LR methods. For CH4, 8% of the fluxes were estimated through LR methods whereas 92% were fitted with HMR methods and for N2O, 17% of the fluxes were calculated with HMR methods and 83% with LR method. Gas mixing ratios (ppm) were converted using the ideal gas law to determine the amount of gas in headspace (on a mole or mass basis), normalized by the surface area of each static flux chamber. Fluxes of CO2 passed all the above data cleaning steps. However, 27.7% of CH4 fluxes (13.3% of soil CH4 fluxes and 57% of stem CH4 fluxes) and 54.4% of N2O fluxes (31% of soil N2O fluxes and 82% of stem N2O fluxes) had to be removed because they did not exceed the detection limit (Parkin et al. 2012).
Soil and stem characteristics
Ancillary environmental variables were simultaneously measured in the soil and tree stems. Soil surface temperature and volumetric water content were recorded at the same time than flux measurements. These measurements were taken at three locations around each collar using a digital waterproof thermometer at 10 cm depth (HI98501, Hanna instruments, UK) and a dielectric soil moisture sensor, with general mineral soil calibration, at 5 cm depth (SM150T, Delta-T Devices, UK). Data of root density, soil organic matter content and pH were from Epron et al. (2006) for the same three topographic positions. In addition, 56 wood samples were taken with a wood cutter of 40 mm in diameter and at DBH.
A 150-mm digital Vernier caliper (Mitutoyo Inc., Japan) was used to measure bark thickness of the wood samples. Wood water content of these samples was calculated with a balance (Sartorius Analytical Balance CPA224, Sartorius AG, Germany; precision = 1.10−4 g) to determine the fresh and dry mass before and after the samples were placed in the oven at 103 °C for 48 hours. Bark and sapwood density of the same samples were the dry biomass in a unit of volume of green wood.
Scaling up
Fluxes of CO2, CH4 and N2O in the tree stems and soil beneath the same trees were scaled up to one hectare by habitat of the studied tropical forest using the stem diameter at DBH, surface area of the five circular plots of 5 m radius within each topographic position and tree basal area. Various challenges can limit the tree flux estimations. The process of extrapolation from plot measurements to regional scale assumes that these plots are representative of the region. Our scaling up was based on four important steps: 1) the three selected habitats were within the footprint of the Guyaflux tower (Fig. 1) and covered 29% (3.5 ha), 45% (5.46 ha) and 25% (3.04 ha) of the surface area for TF, SL and SF, respectively, 2) in each habitat, five circular plots were set up, and tree selection was based on the diameter class of the permanent plots of the Guyaflux unit (Fig. S5). Results of this selection were very closed to values of the natural distribution of the tree community. 3) Calculation of the total surface of the trees was based on the method developed by Chambers et al. (2004), and applied by Rowland et al. (2018), and 4) because of too heterogeneous results from previous studies (Plain et al. 2019; Katayama et al. 2021; Moldaschl et al. 2021; Epron et al. 2022), no vertical pattern was applied for the studied GHGs. The assumption for the stem area flux estimation was that there was a strong functional relationship between total stem surface area (SA) and DBH and total tree stem SA calculation was based on Chambers et al. (2004) equation (Eq. (2)).
where SA is the surface area in m2 and DBH the diameter at breast height in cm. This scaling equation is based on simplified tree forms, and may not accurately represent the diversity of branching structures, which exists in tropical forests. This equation was used to estimate SA for each tree inside the five plots in each habitat. In total, the surface stem for 56 trees varied from 12.25 cm to 100 cm in diameter. Finally, for each habitat, SA was multiplied by the flux of each tree, and the sum of the total stem and soil flux per hectare of habitat was calculated.
For each circular plot, the estimated tree stem fluxes per gas were the sums of SA multiplied by the corresponding gas fluxes of each tree. To determine the exact soil surface area (SS; m2) of each plot, the stem basal areas (m2) were calculated and removed from the plot surface area, 78.5 m2. For each circular plot, estimated soil fluxes were the sums of SS multiplied by the corresponding gas fluxes of each soil collar. Up scaled CO2, CH4 and N2O fluxes from the tree stems and surrounding soils of each forested topographic position, i.e. SF, SL and TF, were then expressed in hectare of forest. The tree stem to soil ratios were calculated for each gas and each forested topographic position.
Statistical analyses
Shapiro - Wilk normality tests were used to determine whether the data were normally distributed (p < 0.05). We tested for differences in GHG fluxes between TF, SL and SF for fluxes of both soil and tree stems using Kruskal - Wallis One Way Analysis of Variance on Ranks. Dunn’s Method was then used to pinpoint which specific means are significantly different from the others (p < 0.05) using pairwise multiple comparison procedures. To obtain more representative GHG fluxes, we averaged the three GHG fluxes per plot (N = 5 for the soil; 2 < N < 7 for tree stems). Data analyses, including descriptive statistics and data visualisation were conducted in the R statistical programming environment (v.3.6.3; R Core Team 2020).
Results
Soil and stem CO2, CH4 and N2O fluxes
Despite a slightly more pronounced soil CO2 emission in SF than in TF (146 ± 39 mgC m−2 h−1 and 124 ± 25 mgC m−2 h−1, respectively), there were no significant differences between the three topographic positions regarding soil CO2 fluxes. In contrast, topographic position had a significant effect (Kruskal - wallis, p < 0.001) on soil CH4 and N2O fluxes. The soil was a net emitter of CH4 in SF (43 ± 149 μgC m−2 h−1) and a CH4 consumer in SL and TF (−13 ± 22 μgC m−2 h−1 and -110 ± 91 μgC m−2 h−1, respectively). Soil CH4 fluxes were significantly different between TF and SL (p < 0.001) and between TF and SF (p < 0.01). N2O fluxes were very low compared with CO2 fluxes. In SF and SL, soils were sources of N2O with 14 ± 23 μgN m−2 h−1 and 11 ± 9 μgN m−2 h−1, respectively. However, the soils in TF acted as sinks for N2O (−15 ± 25 μgN m−2 h−1). N2O fluxes were significantly different between TF and SL (p < 0.001) and between TF and SF (p < 0.05; Fig. 2C).
Topographic position also significantly (Kruskal - wallis, p < 0.001) affected stem fluxes, albeit only for CO2 and N2O fluxes (p < 0.001). Tree stem CO2 emissions were significantly higher in SF than in TF (55 ± 15 mgC m−2 h−1 and 35 ± 5 mgC m−2 h−1, respectively, p < 0.01, Fig. 2D). Stems tended to be sources of CH4 in SF (4 ± 9 μgC m−2 h−1), but not in SL and TF (0 ± 2 μgC m−2 h−1 and 0 ± 11 μgC m−2 h−1, respectively), but the topographic positions did not differ in stem CH4 fluxes. In SL and TF, tree stems consumed N2O (−31 ± 32 μgN m−2 h−1 and -4 ± 18 μgN m−2 h−1, respectively), whereas tree stems emitted N2O in SF (13 ± 13 μgN m−2 h−1). There was a significant difference in N2O fluxes between SL and SF (p < 0.001) and between SL and TF (p < 0.01).
While soils and stems exhibited fluxes of similar direction for CO2 at all topographic positions, this was not the case for the other GHGs. In general, the direction of CH4 fluxes in soils and tree stems was similar, with both acting as sources in SF but exhibiting opposite directions in TF and SL. Specifically, in SF, both soils and tree stems were sources of CH4, while in TF and SL, soil was a sink of CH4 and tree stems were a source of CH4. Nonetheless, in all three habitats both positive and negative stem fluxes occurred. In agreement with CH4 fluxes, SF showed emissions of N2O, while TF showed consumptions of N2O from both soils and stems. In SL, however, soil was a source, while tree stems were a sink of N2O.
Soil and stem characteristics
There were significant differences in soil temperature and soil water content among the three topographic positions (Fig. 3). Soil temperature in TF was significantly higher from the other two topographic positions, while soil water content was significantly different among the three topographic positions. The correlation matrix (Table 2) indicated that soil CH4 fluxes were positively correlated with soil water content and negatively correlated with soil temperature. A significant negative correlation (p < 0.05) was observed between soil temperature and soil N2O flux. Surprisingly, in our study, none of the measured stem traits correlated significantly with the stem GHG fluxes.
Scaling up
For the tree stems, the up-scaled flux rates of CO2, CH4 and N2O to the plot level in each topographic position ranged from 1238 to 1453 gC ha−1 h−1, −67 to 122 mgC ha−1 h−1, and − 67 to −0.9 mgN ha−1 h−1, respectively. Overall, tree stems were mainly a sink of N2O in the three topographic positions, whereas they shifted from sinks to strong sources of CH4 between TF and SF (Supplementary, Fig. S6). In TF, tree stems emitted the equivalent of 73% of the soil CO2 emissions and of 6% and 55% of the soil CH4 and N2O consumptions (Supplementary, Fig. S7). In SF, stem fluxes were 85% of CO2, 28% of CH4 and − 6% of N2O, compared with soil GHG fluxes.
Discussion
This study aimed at understanding whether soil and stem CO2, CH4 and N2O fluxes responded similarly to the changes in environmental conditions across a topographic gradient, and at identifying controls of these fluxes.
Spatial topographic gradient does not affect CO2 emissions
We observed that soil CO2 fluxes did not differ among the three topographic positions, despite the difference in soil water content (factor 3 between SF and TF). Soil CO2 fluxes (81 - 203 mgC m−2 h−1 or 0.51 - 1.28 μmol m−2 s−1) were within the range of values previously reported for French Guianese forests during the wet season (Janssens et al. 1998; Bonal et al. 2008; Rowland et al. 2014; Courtois et al. 2018) or during the transition period between the wet and dry season (Epron et al. 2006; Bréchet et al. 2011). Other studies on the spatial variation in GHG fluxes in tropical forests also reported no effect of topographic position on soil CO2 fluxes (Arias-Navarro et al. 2017; Courtois et al. 2018). The strong spatial heterogeneity in soil CO2 fluxes might be due to the large diversity of tree species within each topographic position (Table 1). Tree species can have a highly different chemical, structural and functional traits of roots and leaves, leading to contrasted litter types, which can influence biogeochemical and physical processes of decomposition related to microbial community activity and, hence, soil GHG fluxes (Townsend et al. 2008; Bréchet et al. 2011; Roland et al. 2013).
Tree stem CO2 emissions on the other hand were significantly different in SF compared to SL and TF (Fig. 2). Stem CO2 fluxes integrate processes of stem growth and stem maintenance respiration (Salomón et al. 2021, 2022), and flux rates depend on the diffusion rates as well as the internal CO2 axial and radial transport (Teskey et al. 2008). According to Saveyn et al. (2008), the transport of respired CO2 in xylem sap from roots to stems, especially under high sap flow rates, is not only a reflection of the rate of actual respiration of the living cells in the woody tissues. Several ecophysiological parameters as sap pH, stem temperature and gas diffusivity in the stem, which can change over time, are likely to have a significant impact on stem CO2 fluxes (Teskey et al. 2008; Trumbore et al. 2013). In this study, we did not find any relationship with bark and wood traits, suggesting that stem CO2 emissions were not necessarily limited by the thickness of the bark (Paine et al. 2010). At a larger scale, however, higher-density bark and sapwood tissues were shown to induce lower stem CO2 fluxes for a given nitrogen mass than lower density tissues (Westerband et al. 2022), which underlines that multiple stem-traits affect their gas exchanges.
Spatial topographic gradient affects CH4 fluxes
Contrary to previous studies (Wolf et al. 2012; Courtois et al. 2018), the topographic transect studied here did influence CH4 fluxes, with soils in SF acting as sources, most likely due to low oxygen, and SL and TF as sinks, most likely due to more aerobic conditions (Table 1). In flooded soil, CH4 is produced under anaerobic conditions (Jeffrey et al. 2020). Soil oxygen concentrations decline with an increase in soil water content, creating favourable conditions for methanogenesis. Concentrations of CH4 in the soil rise, increasing dissolved CH4 in soil water that is subsequently absorbed by tree roots and transported up to the stems.
Emissions of CH4 in tree stems can dramatically increase the source strength of wetland forests and modestly decrease the sink strength of upland forests (Fig. 2E), offsetting the tropical forest carbon sink potential. In TF, aerobic conditions facilitate methanotrophic activity (Hanson and Hanson 1996; Maier et al. 2018; Welch 2018), explaining why CH4 uptake was detected in the upper layer of the soils and the stem fluxes from TF (Table 1). Interestingly, CH4 flux patterns were different between the soil and tree stems (Fig. 4). Some tree stems emitted CH4, while the surrounding soil consumed CH4, suggesting that there is a methanogenic microbial community specific to the tree (Feng et al. 2021) and / or that trees acted as a bypass of the upper soil layer in which all soil-produced CH4 is oxidized. In our study, bark and sapwood traits had no effect on stem CH4 fluxes, in agreement with results in Epron et al. (2022). Pangala et al. (2013) found that CH4 fluxes in tropical tree stems were positively related to stem lenticel density, which was not measured in our study, suggesting that stem fluxes can be constrained by the features of the wood. Further studies are necessary to determine whether other traits such as the chemical composition and porosity of the wood can explain the variations in the stem GHG fluxes.
Spatial topographic gradient affects N2O fluxes
Most of the soil N2O fluxes measured in this study were emissions, except for TF where 75% of the fluxes were consumption. A possible explanation is that SL and SF soils were particularly humid and nitrogen-rich (Ferry et al. 2010). Previous results from other tropical soils showed similar trends concerning nitrogen-rich soil (Arias-Navarro et al. 2017). Nevertheless, soil water content was not linked with N2O fluxes in our study site, as previously reported in Courtois et al. (2018). Several explanations can explain this lack of relationship. First, as the three topographic positions have different clay and sand contents (Epron et al. 2006), soil water content may also differ. Second, soil texture and soil water content at different depths can influence N2O production, with drier soil layers at the surface than deeper in the soil (i.e. 5 cm). Third, N2O can be produced under aerobic conditions by nitrification and can be denitrified to N2, which was not measured in our study. Other soil properties such as total phosphorus and carbon to nitrogen ratio (Butterbach-Bahl et al. 2013) can influence the community composition of microorganisms, but these variables were not measured in our plots. There was no significant relationship between soil water content and N2O emissions from tree stems in our study, which can be explained by the timing and frequency of measurements. In their studies, Machacova et al. (2013) demonstrated that stem N2O emissions peaked 24 hours after rewetting, but then declined rapidly. It is therefore likely that the sampling periods did not always coincide with the maximum denitrification rates.
Scaling up
To our knowledge, flux measurements of simultaneously CO2, CH4 and N2O in mature trees and soil of a highly diverse and heterogeneous tropical forest have never been reported, and it is only recently that trees are recognized as CH4 and N2O flux contributors (Warner et al. 2017; Maier et al. 2018; Welch 2018; Barba et al. 2019; Plain et al. 2019; Machacova et al. 2020; Schindler et al. 2020; Epron et al. 2022). Measuring flux from a single point on a tree stem and extrapolating it to the entire tree has already been described and used in the literature (Machacova et al. 2016; Warner et al. 2017). Indeed, results from tree stem GHG flux studies are highly variable not always shown clear pattern across a vertical profile (Chambers et al. 2004; Epron et al. 2022; Katayama et al. 2014, 2021; Plain et al. 2019). In this study, we measured GHG flux at DBH and, while there are potential drawbacks to this extrapolation, such as oversimplification of flux upscaling, we believe it is a useful initial global approach.
In SF, where the flux differences were the highest, stems contributed up to 22% to total CH4 emission (soil + stems) and in SL stems contributed up to 43% to total N2O consumption. This showed that tropical tree stems cannot only emit carbon through CH4 fluxes, but also take up a certain quantity of nitrogen from the atmosphere through N2O fluxes. Nevertheless, interpretation of our scaling up approach should be made with caution due to the absence of repetitions over time, relatively small surface area (circular plots were 78 m2, covering 393 m2 of each forested topographic position) and rather simple allometric regression model for estimating the total tree stem surface area per plot. Since we carried out the flux measurements during the wet season, we assumed that the emissions of the stem CO2, CH4 and N2O were not affected by lack of water into the soil, which can promote a decrease in the intensity of the transpiration stream and, hence, affect the transport of CH4 and N2O. In the soil, CO2, CH4 and N2O fluxes are known to be highly heterogeneous due to highly variable physical, chemical and biological properties (Arias-Navarro et al. 2017; Courtois et al. 2018), whereas changes in stem CO2, CH4 and N2O fluxes due to tree individuals and tree species traits remain poorly documented, especially for CH4 and N2O in tropical forest. Temporal variation in CO2, CH4 and N2O fluxes in the stems and soils is also important to take into account when upscaling fluxes.
Conclusion
In the wet season conditions, our results not only revealed that tree stems accounted for non-negligible ecosystem GHG fluxes, but also that stems and the surrounding soils shifted from sinks to sources of CH4 and N2O along a topographic transect, while both remaining a source of CO2. Soil CH4 and N2O fluxes differed among topographic positions with consistently higher CH4 and N2O fluxes in SF. Tree stem CO2 and N2O fluxes also differed among topographic positions, with higher CO2 emission in SF and a pronounced stem N2O consumption in SL. In our tropical forest site, temperature and soil water content were important environmental factors for soil N2O fluxes, while soil water content was the main driver of soil CH4 fluxes.
Being common in the Guiana shield and many other tropical areas, taking into account the effect of these topographic patterns can be relevant for modelling the tropical forest GHG budgets. The variation in CO2, CH4 and N2O fluxes remained mostly unexplained, highlighting their high spatial and temporal variation. Despite the analysis of several wood traits, none of them explained the observed variations in stem CO2, CH4 and N2O fluxes. Additional studies are thus required to disentangle the effect of the soil properties and tree stem traits on GHG fluxes. Future research in tropical forest is also necessary to determine which drivers control the temporal variations in tree stem GHG fluxes, knowing that intra-seasonal variations can influence the contributions of the trees to local and global GHG flux budgets.
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Acknowledgements
This work was supported by the European Research Council Synergy grant ERC-2013-SyG-610028-IMBALANCE-P, the European Commission through a Marie Skodowska-Curie Individual Fellowship H2020-MSCA-IF-2017-796438 to LMB, the Research Fund of the University of Antwerp and an “Investissement d’Avenir” grant delivered by the Agence Nationale de la Recherche (CEBA; ANR-10-LABX-25-01). We thank CIRAD - UMR EcoFoG for hosting our fieldwork.
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Daniel, W., Stahl, C., Burban, B. et al. Tree stem and soil methane and nitrous oxide fluxes, but not carbon dioxide fluxes, switch sign along a topographic gradient in a tropical forest. Plant Soil 488, 533–549 (2023). https://doi.org/10.1007/s11104-023-05991-y
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DOI: https://doi.org/10.1007/s11104-023-05991-y