Abstract
Caffeine is commonly used in Dictyostelium to inhibit the synthesis of the chemoattractant cAMP and, therefore, its secretion and the autocrine stimulation of cells, in order to prevent its interference with the study of chemoattractant-induced responses. However, the mechanism through which caffeine inhibits cAMP synthesis in Dictyostelium has not been characterized. Here, we report the effects of caffeine on the cAMP chemoattractant signaling network. We found that caffeine inhibits phosphatidylinositol 3-kinase (PI3K) and mechanistic target of rapamycin complex 2 (mTORC2). Both PI3K and mTORC2 are essential for the chemoattractant-stimulated cAMP production, thereby providing a mechanism for the caffeine-mediated inhibition of cAMP synthesis. Our results also reveal that caffeine treatment of cells leads to an increase in cAMP-induced RasG and Rap1 activation, and inhibition of the PKA, cGMP, MyoII, and ERK1 responses. Finally, we observed that caffeine has opposite effects on F-actin and ERK2 depending on the assay and Dictyostelium strain used, respectively. Altogether, our findings reveal that caffeine considerably affects the cAMP-induced chemotactic signaling pathways in Dictyostelium, most likely acting through multiple targets that include PI3K and mTORC2.
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Introduction
Caffeine is a purine alkaloid that is lipid soluble, allowing it to easily cross biological membranes. In humans, caffeine acts as a non-competitive antagonist of adenosine receptors but also inhibits phosphodiesterases and several protein kinases of the phosphatidylinositol 3-kinase (PI3K)-related kinase (PIKK) family, including PI3K and mechanistic target of rapamycin (mTOR) [1, 2]. In yeasts, caffeine inhibits the mTOR complex 1 (mTORC1) [3].
In Dictyostelium, early studies of the role of cAMP in development led to the identification of caffeine as a compound that inhibits cAMP synthesis, with a maximal effect at ~ 3 mM [4]. In Dictyostelium, cAMP acts both as an extracellular chemoattractant and an intracellular second messenger [5]. Caffeine was found to only marginally affect cAMP binding to its cell surface receptors but to efficiently inhibit adenylyl cyclase activity and cAMP production in an indirect and reversible fashion [4, 6]. Efforts at defining the mechanism through which caffeine inhibits cAMP synthesis in Dictyostelium led to the suggestion that there are at least two different targets of caffeine, with at least one of them downstream from the heterotrimeric G protein Gα2, which couples to the main cAMP receptor, cAR1 [7,8,9,10]. Although the targets of caffeine in Dictyostelium remain unknown, caffeine continues to be widely used by Dictyostelium researchers to inhibit cAMP synthesis and, thereby, prevent the autocrine stimulation of cells in studies of cAMP chemoattractant signaling.
Much is now known about the cAMP chemoattractant signal transduction network in Dictyostelium, which includes central PI3K and mTOR complex 2 (mTORC2) pathways (Fig. 1) [11]. Interestingly, input from both of these pathways is necessary for adenylyl cyclase A (ACA) activation and cAMP production in response to extracellular cAMP stimulation [12,13,14,15]. In addition, we have found that many of the upstream components of the cAMP chemotactic signaling cascades are regulated by downstream kinases through negative feedback loops. We have shown that AKT/Protein Kinase B (PKB) and PKB-related kinase 1 (PKBR1) inhibit the upstream RasC-mTORC2 pathway, and that Protein Kinase A (PKA), which is activated by intracellular cAMP, negatively regulates the activity of upstream RasG, Rap1, and mTORC2 pathways (Fig. 1) [16, 17].
Dictyostelium is a widely used experimental model for studying cell migration, chemotaxis, and chemoattractant signaling pathways, and much of what we know today about the signaling pathways and mechanisms implicated in the directed migration of eukaryotic cells was originally discovered in Dictyostelium [18]. However, since caffeine is widely used in Dictyostelium chemotaxis studies without knowledge of its mechanism of action, we wondered if caffeine could be changing the chemotactic responses and, thus, affect the interpretation of the data obtained in its presence. Therefore, the present study was undertaken to characterize the effects of caffeine on cAMP chemoattractant signal transduction in Dictyostelium. Our findings suggest that caffeine inhibits PI3K and mTORC2, which explains many of the effects of caffeine on chemoattractant signaling, including its inhibition of cAMP synthesis. In addition, our work shows that caffeine differentially affects the F-actin polymerization response and ERK2 activity depending on the assay or the Dictyostelium strain used, respectively.
Materials and methods
Reagents
cAMP sodium salt monohydrate, 2′-deoxyadenosine-5′-monophosphate (2′-deoxy-cAMP) disodium salt, caffeine powder, protein kinase A (PKA), and anti-Flag M2 were from Sigma-Aldrich (St. Louis, MO, USA). H2B was from Roche-Genentech (San Francisco, CA, USA) and Geneticin was purchased from Life Technologies (Grand Island, NY, USA). Torin2 was purchased from ApexBio (Houston, TX, USA). Phospho-p70 S6 kinase (Thr389; 1A5), phospho-Akt substrate (110B7), phospho-(Ser/Thr) PKA substrate, pan-phospho-PKC (zeta Thr410; 190D10), and phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204) antibodies were from Cell Signaling Technology (Danvers, MA, USA). Pan-Ras antibody (Ab-3; RAS10) was from Calbiochem/EMD Millipore (Billerica, MA, USA). ERK1 antibody (C16; sc-93) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). EMD Millipore/Novagen T7.Tag™ monoclonal antibody and antibody agarose, as well as EMD Millipore/Calbiochem PANSORBIN™ cells, were purchased from Fisher Scientific (Waltham, MA, USA). The Rap1 (directed against amino acids 169–182 of Dictyostelium Rap1) was custom-made by ProSci Incorporated (Poway, CA, USA). HRP-conjugated secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA). PKB and PKBR1 antibodies, Lifeact-GFP, GFP-MyoII, and PHcrac-GFP constructs were gifts from Rick Firtel and were previously described [19,20,21,22]. Flag-tagged RasC construct was reported elsewhere [17]. T7-tagged Pianissimo (T7-Pia) was cloned by ligating AvrII-digested restriction sites into the compatible SpeI site of the extrachromosomal vector pDM304, which was obtained from the Dicty Stock Center [23] (depositor: Douwe Veltman; GenBank Accession Number EU912539), using the following primers: T7-Pia Forward, AAGTGCCTAGGAAAAAATGGCATCAATGACAGGTGGTCAACAAATGGGTAGAATGACAAGTTCTGATAGTAGTGTAAATACTACATCG; Pia reverse, AAGTGCCTAGGTTAATTTAAATCATGATATGGATCAGATGAAAATATTGCAACATC.
Cell culture and strains used
Dictyostelium cells were grown attached to substrate in axenic HL5 medium (ForMedium, Hunstanton, Norfolk, UK) at 22 °C and transformants were generated by electroporation. Transformed cells were selected in 20 µg/ml Geneticin and confirmed by immunoblot. The wild-type strain used was AX3, and we also used AX2 where indicated. piaA null cells were generously provided by Peter Devreotes (Johns Hopkins University, Baltimore, MD) and described elsewhere [14]. For all assays, cells were developed by pulsing with 30 nM cAMP every 6 min for 5.5 h in 12 mM Na/K phosphate buffer (pH 6.1) at a confluency of 5 × 106 cells/ml in a shaking suspension culture. Prior to assays, developed cells were washed twice with 12 mM Na/K phosphate buffer followed by 30-min incubation with or without 5 mM caffeine and stimulation with 1 µM cAMP.
Biochemical assays and immunoblots
F-actin measurements using Phalloidin staining, PKB and PKBR1 kinase assays, Ras-GTP and Rap1-GTP pull-down assays were performed as previously described [20,21,22, 24,25,26]. Phosphorylation of PKB and PKBR1, ERK1 and ERK2, PKB substrates, and PKA substrates from total cell lysates was detected by immunoblot. Activation loop (AL) phosphorylation of PKB (T278) and PKBR1 (T309) was detected using pan-phospho-PKC antibody diluted 1:1000. Hydrophobic motif (HM) phosphorylation of PKB (T435) and PKBR1 (T470) was detected using phospho-p70 S6 kinase antibody diluted 1:1000. Phospho-ERK1 and -ERK2 were detected using phospho-p44/42 MAPK antibody diluted 1:5000. PKB and PKA substrate phosphorylation was detected using phospho-Akt substrate and phospho-(Ser/Thr) PKA antibodies, respectively, diluted 1:2000 as described previously [17].
cAMP was measured using a protocol adapted from [27]. Briefly, developed cells were stimulated with 10 µM 2′-deoxy-cAMP for the time indicated, deproteinized using perchloric acid (1.75%) precipitation followed by neutralization with 50% bicarbonate. Samples were then incubated with 0.01 µCi [3H]cAMP and 5 ug of PKA in assay buffer (100 mM K2HPO4/KH2PO4, 10 mM EDTA, 2 mg/ml BSA, 3 mM NaN3, pH 7.0) for 2 h in ice water. Reactions were stopped by adding equal volume of 5% charcoal and incubating 1 min before centrifuging to sediment the charcoal. The supernatants were then transferred to scintillation vials for counting. Results were calculated as previously described [27].
cGMP was measured using a cGMP Enzyme Imunoassay Kit (Sigma-Aldrich). After cAMP stimulation, samples were deproteinized using trichloroacetic acid (10%) precipitation followed by neutralization with 50% bicarbonate. Samples were then diluted 1:10 with the assay buffer before cGMP was measured according to the manufacturer’s protocol.
To measure mTORC2 kinase activity in vitro, 1 × 108 developed T7-Pia/piaA null and piaA null cells were stimulated or not with 1 µM cAMP for 10 s and lysed in lysis buffer (final concentrations: 20 mM HEPES pH 7.5, 60 mM NaCl, 0.5 mM EDTA, 0.15% CHAPS, 5 mM Na-pyrophosphate, 5 mM β-glycerophosphate, 25 mM NaF, 0.25 mM Na-orthovanadate, 2.5 µg/ml aprotinin, and 2.5 µg/ml leupeptin) for 30 min at 4 °C with shaking. Lysates were cleared by centrifugation at 20,000×g 10 min. Supernatants were collected, proteins quantified and adjusted to have 2 µg/µl × 1 ml lysates. Lysates were then precleared with PANSORBIN™ cells 30 min at 4 °C before incubation with T7.Tag™ antibody agarose for 4 h at 4 °C with rocking. Immunoprecipitates were washed four times in lysis buffer followed by one wash in kinase buffer (25 mM HEPES, 0.1 M potassium acetate, 1 mM MgCl2). The purification of mTORC2 by T7-Pia pull-down was confirmed by mass spectrometry. Immunopurified mTORC2 was then incubated or not with 5 mM caffeine or 1 µM Torin2 for 30 min at 22 °C before assaying the kinase activity of mTORC2 using piaA null cell lysates as substrate. This “substrate” piaA null cell lysates was produced by mechanical disruption of the cells using Polytron homogenization. For the kinase reaction, immunopurified mTORC2 was incubated in 15 µl kinase buffer containing 1.5 mM ATP and 30 µg piaA null cell lysates, for 15 min at 22 °C with frequent mixing. Kinase reactions were stopped by adding 45 µl of SDS-PAGE sample buffer and the in vitro mTORC2-mediated phosphorylation of PKB and PKBR1 in the piaA null cell lysates was detected by immunoblot using the phospho-p70 S6 kinase antibody.
Imaging
Assessment of global responses of fluorescent reporters to cAMP stimulation, and image acquisition were performed as previously described [16, 25, 26, 28]. Images were acquired using a Marianas Spinning Disk Confocal Workstation (Intelligent Imaging Innovations, Inc., Denver, CO, USA) equipped with an Evolve™ 512 EMCCD camera (Photometrics, Tucson, AZ, USA), and image analysis was performed using the Slidebook software (Intelligent Imaging Innovations, In., Denver, CO, USA). Analysis of fluorescent reporter translocations between the cytosol and the cell cortex was performed by measuring changes in fluorescent intensities in the cytosol normalized to each cell’s basal levels, as described previously [29]. To assess the reversibility of caffeine inhibition of PI3K, developed cells plated on 35-mm dishes, with coverslips for imaging, were treated with caffeine for 30 min, washed twice with NaK phosphate buffer, and then stimulated with cAMP while imaging the PHcrac-GFP translocation response ~ 10 min after caffeine removal.
Results
Caffeine inhibits PI3K and mTORC2 activity
Since caffeine inhibits PI3K and mTOR in mammalian cells, and PI3K and mTORC2 play key roles in cAMP chemoattractant signaling in Dictyostelium, we started by assessing the effect of caffeine on these two kinases. For PI3K, we evaluated the effect of caffeine on the cAMP-induced, PI3K-mediated production of phosphatidylinositol (3,4,5)-triphosphate [PI(3,4,5)P3] using the PI(3,4,5)P3 fluorescent reporter PHcrac-GFP [30, 31]. PHcrac-GFP is mostly cytosolic in resting cells and translocates to the plasma membrane of control cells upon cAMP stimulation, reflecting the production of PI(3,4,5)P3 and, thereby, PI3K activation (Fig. 2a). By contrast, only a fraction of the PI(3,4,5)P3 reporter was recruited to the plasma membrane in caffeine-treated cells. However, the cAMP-induced translocation of PHcrac-GFP in caffeine-treated cells was recovered shortly after the removal of caffeine (Fig. 2b). Therefore, these observations suggest that caffeine inhibits the cAMP-induced activation of PI3K in a reversible manner.
To investigate if caffeine inhibits mTORC2 in Dictyostelium, we assessed the effect of caffeine on the cAMP-induced and mTORC2-mediated PKB and PKBR1 phosphorylation [32, 33]. In Dictyostelium, mTORC2 phosphorylates PKB and PKBR1 at their C-terminal hydrophobic motif (HM; PKBT435 and PKBR1S470) and this phosphorylation is required for the PDK1-mediated phosphorylation of the PKB kinases in their activation loop (AL; PKBT278 and PKBR1T309) [33]. PI3K is also implicated in promoting PKB activation through the production of PI(3,4,5)P3 that mediates the recruitment of PKB to the plasma membrane [34]. PKBR1 is anchored at the membrane through myristoylation and its phosphorylation and activation is independent of PI3K [33]. As expected, control cells display strong and transient cAMP-induced PKB- and PKBR1-AL and -HM phosphorylation (Fig. 2c). On the other hand, caffeine-treated cells exhibit considerably reduced cAMP-stimulated PKB and PKBR1 phosphorylation at both the AL and HM sites. In addition, similar to the PI3K response, we observed the rescue of PKB and PKBR1 phosphorylation shortly after the removal of caffeine, although the phosphorylation of PKB at its AL and HM sites is not fully recovered (Fig. 2d). Together, these observations suggest that caffeine inhibits the cAMP-induced activation of mTORC2 in a mostly reversible manner.
Since other intracellular factors control the phosphorylation state of the PKB kinases in addition to mTORC2, we also more directly tested the effect of caffeine on the kinase activity of mTORC2 in vitro. Using the mTORC2-specific component Pianissimo (Pia) tagged to T7 and expressed in piaA null cells (Online Resource 1 a and b), we purified mTORC2 from developed cells stimulated with cAMP. Purified mTORC2 was then incubated with 5 mM caffeine, or 1 µM of the mTOR inhibitor Torin2 as control (Online Resource 1 c), before incubation with piaA null cell lysates serving as a source of PKB and PKBR1 substrates [35]. The kinase activity of purified mTORC2 was then evaluated by revealing its phosphorylation of PKB and PKBR1 by immunoblot. We observed that caffeine treatment of immunopurified mTORC2 inhibits its kinase activity towards PKB and PKBR1 in vitro to a similar extent as Torin2 (Fig. 2e). Therefore, this result suggests that caffeine directly inhibits mTORC2.
We then assessed the kinase activity of PKB and PKBR1 as well as their downstream phosphorylation of substrates in cells. As could be expected, caffeine-treated cells display considerably reduced cAMP-induced PKB and PKBR1 kinase activity (Fig. 2f), as well as reduced PKB and PKBR1 cellular substrate phosphorylation, as detected by immunoblot using an anti-phospho-PKB substrate (P-PKBS) antibody (Fig. 2g). The phosphorylated protein detected by the P-PKBS antibody at ~ 25 kDa that is unaffected by caffeine treatment has previously been shown to be phosphorylated independently of the PKB kinases [34]. Altogether, these observations suggest that, similar to findings in mammalian cells, caffeine inhibits PI3K and mTORC2 in Dictyostelium, and that this leads to a decrease in PKB and PKBR1 signaling.
Effects of caffeine on other chemotactic pathways
We then investigated the effect of caffeine on other pathways, whether they are already known to be linked or not to PI3K and mTORC2 signaling in Dictyostelium, to better characterize the outcome of caffeine treatment on cAMP signaling. We compared the activity of Protein Kinase A (PKA; activated by cellular cAMP) and Extracellular-Regulated Kinases 1 and 2 (ERK1 and ERK2), as well as the production of the second messenger cGMP, in cells treated or not with 5 mM caffeine. With the lack of a readily available commercial PKA kinase activity assay that can be used with Dictyostelium, we assessed PKA activation by evaluating the phosphorylation of PKA substrates by immunoblot using a phospho-PKA substrate antibody. We stimulated the cells with cAMP and, to circumvent the potential intake of extracellular cAMP that could directly bind and activate intracellular PKA, we also assessed PKA activity in response to stimulation with the cAMP analog 2′-dcAMP, which is an agonist for cAR1 but cannot bind and activate PKA directly [36]. We observed that caffeine-treated cells display reduced cAMP- and 2′-dcAMP-induced phosphorylation of cellular PKA substrates compared to non-treated cells (Fig. 3a, b). This result indicates that caffeine treatment of cells inhibits PKA activation in response to cAMP chemoattractant stimulation, consistent with the caffeine-mediated inhibition of cAMP production (Fig. 3c) [4].
To determine the effect of caffeine on the cAMP-induced activation of ERK1 and ERK2, we evaluated the kinases’ phosphorylation as a measure of their activity, as previously reported [37]. Interestingly, when we first assessed ERK1 and ERK2 phosphorylation, we reproducibly observed differences in their profiles whether we used AX2 or AX3 wild-type strains (Fig. 3d). For ERK1, we observed much higher phosphorylation levels in AX3 than in AX2 cells, particularly in resting, non-stimulated cells. However, ERK1 is similarly inhibited by caffeine in both AX2 and AX3 cells: basal ERK1 phosphorylation levels are considerably reduced and the cAMP-induced phosphorylation of ERK1 is delayed in caffeine-treated cells compared to control cells (Fig. 3d). For ERK2, whereas its phosphorylation profile is similar in both AX2 and AX3 untreated cells, the effect of caffeine is completely different: caffeine-treated AX2 cells display reduced cAMP-induced ERK2 phosphorylation while caffeine-treated AX3 cells consistently display increased and prolonged cAMP-induced ERK2 phosphorylation compared to control cells (Fig. 3d). Together, these observations suggest that caffeine inhibits the cAMP-induced ERK1 activation and that the effect of caffeine on ERK2 considerably differs with the strain used.
We then defined the effect of caffeine on the cAMP-induced production of cGMP, which is also associated with the chemotactic response to cAMP in Dictyostelium [38]. Whereas little is known about the cGMP pathway in Dictyostelium, evidence suggests the potential involvement of small GTPases RasC and RasG in promoting cGMP production in response to cAMP chemoattractant stimulation (Fig. 1) [39]. Comparing caffeine-treated and non-treated cells, we measured the cGMP levels in resting conditions (basal) as well as 5 and 12 s after cAMP stimulation (Fig. 3e). As previously reported, control cells display a cAMP-induced increased in cGMP reaching ~ 25 times over basal levels at 12 s after stimulation [39]. Caffeine-treated cells also produced cGMP in response to cAMP stimulation, but only up to ~ 10 times over basal levels (Fig. 3e). Thus, this observation indicates that caffeine treatment of Dictyostelium cells reduces the cAMP-induced cGMP response.
Effects of caffeine on cytoskeleton regulation
Since PI3K and mTORC2 pathways regulate F-actin, and cGMP regulates Myosin II (MyoII) in chemotaxing Dictyostelium cells, we next assessed the effect of caffeine on the cAMP-induced F-actin polymerization and MyoII assembly responses. To measure F-actin, we used two different assays: (1) the imaging of adherent cells expressing the F-actin reporter Lifeact-GFP [40]; and (2) the TRITC-Phalloidin labeling of F-actin in cells in suspension. As previously reported, non-treated cells display a typical biphasic cAMP-stimulated F-actin polymerization response with a first sharp peak at ~ 5 s, which associated with initial actin reorganization (cringe response), and a second broader peak at ~ 40–60 s, which is associated with pseudopod protrusions (Fig. 4a, b) [41]. Interestingly, we observed opposite effects of caffeine on the F-actin responses in the two different assays. In Lifeact-GFP imaging, caffeine-treated cells display reduced translocation of the F-actin reporter to the cell cortex upon cAMP stimulation, suggesting reduced F-actin polymerization (Fig. 4a). On the other hand, measurement of F-actin using TRITC-Phalloidin labeling shows increased F-actin polymerization in caffeine-treated compared to non-treated cells (Fig. 4b). We do not know why caffeine has different effects on F-actin polymerization depending on the assay used, but different possibilities are considered in Discussion section.
To assess the effect of caffeine on MyoII dynamics in response to cAMP stimulation, we used cells expressing GFP-fused myosin heavy chain A (GFP-MyoII) [28]. As reported previously, GFP-MyoII localizes to the cell cortex in resting cells and translocates to the cytosol upon cAMP stimulation, which is then followed by an increase in cortical GFP-MyoII, with a peak ~ 40–50 s after cAMP stimulation, before returning back to basal levels (Fig. 4c). By comparison to non-treated cells, caffeine-treated cells displayed a greater initial displacement of cortical GFP-MyoII to the cytosol, and then directly and slowly returned to basal levels at the cell cortex ~ 50 s post-stimulus. Thus, this result suggests that caffeine decreases the cAMP-induced assembly of MyoII at the cell cortex.
Effects of caffeine on the upstream Ras and Rap1 responses
The Ras and Rap1 GTPases are activated early in the cAMP chemoattractant-induced signaling pathways, upstream of PI3K and mTORC2. However, RasC, RasG, and Rap1 are also linked to PI3K and mTORC2 through feedback loops (Fig. 1) [16, 22, 42, 43]. We therefore assessed the effect of caffeine on the cAMP-induced activation of RasC, RasG, and Rap1. For RasC, cAMP stimulation induces its transient activation with a peak at 5–10 s and a return to basal levels after ~ 40 s (Fig. 5a). We did not observe any meaningful effect of caffeine on basal nor cAMP-stimulated RasC activity. For RasG, control cells display a 5-s peak of cAMP-induced activation with a rapid return to basal levels by 20 s after stimulation (Fig. 5b). By contrast, while we observed similar basal RasG activity levels in caffeine-treated cells compared to control cells, cAMP stimulation produced a ~ twofold stronger RasG activation response in caffeine-treated cells. After this initial response, RasG activity is then reduced by ~ 50% at 20 s after cAMP stimulation and remains at this level at least until 60 s after stimulation. For Rap1, cAMP stimulation of control cells induces its activation with a peak at ~ 5–10 s and a ~ 50% reduction in activity at ~ 20 s that persists afterwards at least until 60 s after stimulation (Fig. 5c). In caffeine-treated cells, we observed increased basal levels as well as cAMP-stimulated Rap1 activity at 5, 10, and 20 s after stimulation, with a return to basal levels after 40 s. Altogether, these observations show that treating Dictyostelium cells with caffeine, while not affecting RasC activity, leads to stronger RasG and Rap1 responses and changes their profiles.
Discussion
We have characterized the effect of caffeine on chemoattractant signaling in Dictyostelium and our findings suggest that it includes the inhibition of PI3K and mTORC2. Both enzymes are necessary for ACA activation in response to chemoattractant stimulation, thereby explaining the inhibitory effect of caffeine on cAMP synthesis (Fig. 6). In addition, considering the known cAMP chemoattractant signaling pathways, caffeine inhibition of PI3K and mTORC2 also explains several of the other observed effects of caffeine in Dictyostelium. These effects include caffeine’s inhibition of PKB, PKBR1, and PKA activity, as well as its positive effect on RasG and Rap1, possibly due to the PKA-mediated negative feedback loops (Fig. 6). In addition, the observation that caffeine mostly affects the amplitude of the responses measured and not their timing could indicate that some PI3K and mTORC2 are still active in cells treated with 5 mM caffeine and that substrates are in excess, and thus, that PI3K and mTORC2 signaling may not, or minimally, be govern by a threshold. On the other hand, the lack of an effect of caffeine on RasC activity, as well as its effects on cGMP, ERK1, ERK2, F-actin, and MyoII are more intriguing and suggest either that PI3K and/or mTORC2 play other, unknown roles in the chemotactic signaling network or that caffeine also targets other proteins in the network.
We were surprised to observe that caffeine treatment of cells inhibits the cAMP-induced production of cGMP, since a previous study reports no effects of caffeine on the cGMP response [4]. This discrepancy may lie in the fact that different Dictyostelium strains were used. This is highly possible since we observed, for example, that the effect of caffeine on the ERK2 response varies considerably between the AX2 and AX3 strains. Indeed, whereas caffeine inhibits the basal and early cAMP-induced phosphorylation of ERK1 in both AX2 and AX3 cells, the cAMP-induced ERK2 phosphorylation response is reduced in AX2 but increased in AX3 cells treated with caffeine. The mechanisms through which caffeine affects ERK1 and ERK2 activity are unknown, but the potentiating effect of caffeine on ERK2 in AX3 cells could partly be due to reduced PKA activity, which was previously suggested to negatively regulate ERK2 [44]. Although evidence suggest that intracellular cAMP-PKA signaling may not mediate adaptation of ERK2 as previously expected [45], a role for PKA in regulating the extent of ERK2 activation is not excluded.
Another unexpected observation is the effect of caffeine on the cAMP-induced F-actin polymerization. First, the underlying cause for the opposite effects of caffeine on the F-actin response measured by Lifeact-GFP imaging versus Phalloidin labeling is unknown, but we speculate that this is due to the different cellular conditions. The imaging is performed on attached cells, whereas Phalloidin labeling is performed after stimulation of cells in suspension. This is an important observation, as it suggests that responses to chemoattractant stimulation of Dictyostelium cells may considerably vary whether the cells are in suspension or attached to a substrate. In addition, the increased cAMP-induced F-actin polymerization observed in cells in suspension is very counterintuitive since the expected targets of caffeine, PI3K and mTORC2, are involved in promoting the F-actin response [13, 46] (Fig. 6). Therefore, this observation suggests that, under these conditions, there is another caffeine target responsible for this effect. On the other hand, the inhibitory effect of caffeine on MyoII assembly likely results from the decrease in cGMP production in caffeine-treated cells.
In addition to the observations presented herein, in a previous study, we showed that caffeine also affects the cAR1-mediated heterotrimeric G protein activation, specifically increasing the potency of the cAMP-induced G protein subunit dissociation [47]. We do not think that the effect of caffeine on G protein subunit dissociation plays a role in the other effects of caffeine reported therein, since all the experiments were performed using saturating concentrations of the cAMP stimulus (1 µM) and that, at this concentration, caffeine displays no significant effect on G protein subunit dissociation. The mechanism through which caffeine affects G protein activation remains unknown, including whether it involves PI3K and/or mTORC2, but this observation suggests that caffeine affects a regulator of G protein activation.
Early studies of the effect of caffeine in Dictyostelium have shown that caffeine inhibits endocytosis, particularly pinocytosis at 1.5–6 mM caffeine, in addition to cAMP production [48]. Caffeine-mediated inhibition of PI3K could partly explain the effect of caffeine on pinocytosis since PI3K is involved in this process [49]. Previous studies also suggest that caffeine inhibits the Dictyostelium phosphodiesterase 4 (PDE4), the extracellular PDE responsible for degradation of secreted cAMP during development [7]. Caffeine also inhibits PDEs in human cells and could inhibit intracellular PDEs in Dictyostelium as well, but these are unlikely major targets of caffeine since cAMP and cGMP levels are reduced, and not increased as would be expected from PDE inhibition, in caffeine-treated cells [1]. However, there are likely other proteins and cAMP chemoattractant responses that are affected by caffeine that we have not tested for. For example, we expect that the activation of Rho GTPases may be inhibited, since some of them are known to lie downstream from PI3K [11].
In conclusion, our study reveals that caffeine inhibits PI3K and mTORC2 activity and downstream signaling in Dictyostelium, and that caffeine also differentially affects the cAMP-induced production of cGMP, the activation of RasG, Rap1, ERK1, and ERK2, as well as the F-actin and MyoII responses. Although there are likely several caffeine targets responsible for the observed effects, given our observations and evidence from mammalian studies that caffeine directly inhibits PIKK family kinases, we propose that the effect of caffeine in Dictyostelium involves its direct targeting of PI3K and mTORC2. Therefore, these effects need to be considered when caffeine is used in studies of the Dictyostelium chemoattractant signaling network.
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Acknowledgements
We are grateful to the Dicty Stock Center and its material depositors for providing cells and DNA constructs.
Funding
This study was funded by a Research Scholar Grant 127940-RSG-15-024-01-CSM from the American Cancer Society to P.G.C. M.S. was supported by NIH T32 Grant GM008804 and P.L. was supported by a U.S. Public Health Service Grant GM037830.
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Tariqul Islam, A.F.M., Scavello, M., Lotfi, P. et al. Caffeine inhibits PI3K and mTORC2 in Dictyostelium and differentially affects multiple other cAMP chemoattractant signaling effectors. Mol Cell Biochem 457, 157–168 (2019). https://doi.org/10.1007/s11010-019-03520-z
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DOI: https://doi.org/10.1007/s11010-019-03520-z