Abstract
Magnetic nano- and microparticles have been successfully used in many areas of algae biotechnology, especially for harvesting of algal biomass, separation of algal biologically active compounds, immobilization of algal cells, removal of important xenobiotics using magnetically modified algae, or for the preparation of magnetic catalysts; alternatively, algae have been employed for the production of magnetic iron oxide nanoparticles. In this short review paper, the relevant data published in the period 2016–2019 are summarized.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Introduction
Magnetically responsive materials are typical examples of smart materials exhibiting several types of interaction with external magnetic field (Safarik et al. 2012). Such materials have found many important applications in almost any area of biosciences, biotechnology, and environmental technology, including also algae research and (bio)technology.
Magnetic nano- and microparticles have been successfully used for harvesting of microalgal biomass from cultivation media, magnetic labeling of microalgal cells, magnetic separation of microalgal biologically active compounds, magnetic detection of algae toxins, and preparation of magnetically responsive catalysts applicable in microalgal biotechnology. Magnetically modified microalgal cells have been employed as efficient biosorbents of important pollutants or part of whole-cell biosensors. Despite the fact that there is a real boom of studies employing magnetic particles for microalgae separation from large volumes, other areas of algal research and (bio)technology have not fully employed the potential offered by magnetically responsive materials.
This topic has been reviewed recently in detail by the authors of this paper (Safarik et al. 2016b, 2017). Currently, new scientific studies focusing on magnetic particles in combination with micro- and macroalgae have been performed in this interdisciplinary research. In this short review, new data published mainly in the period 2016–2019 (not presented in above mentioned review papers) are summarized. We hope that this paper will stimulate the microalgae and macroalgae research community in finding further progressive applications of magnetically responsive materials.
Magnetic harvesting (flocculation) of microalgae
Recovery and harvesting of microalgal biomass represent a critical bottleneck of any large-scale algal biotechnology process. There are various harvesting techniques available (e.g., centrifugation, sedimentation, filtration, ultrafiltration, and flotation; Milledge and Heaven 2013), but the flocculation-based processes have acquired much attention due to their promising efficiency and scalability (Matter et al. 2019). Flocculation with the aid of magnetic particles is of high interest; magnetic particles are highly attractive harvesting agents due to their biocompatibility, efficiency, non-destructive nature of the magnetic field, easy manipulation, and potential regeneration. Ideal magnetic particles should be of low cost, easy to prepare, stable, and reusable. Several parameters are usually studied to optimize the flocculation process, including pH, magnetic (nano)particle dosage, temperature, composition of culture media, and conditions for the release of cell bound particles (Seo et al. 2017; Matter et al. 2019). The examples of recently published approaches are shown in Table 1.
Naked magnetic (nano)particles have often been used for microalgae cell flocculation, including commercially available magnetite nanoparticles (NPs) for Chlorella vulgaris (Zhu et al. 2019), microwave-synthesized magnetite microparticles for Nannochloropsis oceanica (Boli et al. 2017), or magnetite NPs prepared by coprecipitation of Fe2+ and Fe3+ at high pH for Scenedesmus ovalternus and Chlorella vulgaris (Fraga-Garcia et al. 2018). In specific cases also rather exotic magnetic flocculants, e.g., commercially available yttrium iron oxide (Y3Fe5O12) NPs have been successfully used for C. vulgaris (Zhu et al. 2019). Surface-modified magnetite particles also have been prepared and efficiently employed for algae flocculation; positively charged polyethylenimine–magnetite particles were used for magnetic harvesting of negatively charged Chlorella zofingiensis, C. vulgaris, Chlorella sorokiniana, Chlorella ellipsoidea and Botryococcus braunii (Gerulová et al. 2018). Also, flocculation of different types of Microcystis aeruginosa was studied using polyethylenimine-coated magnetic nanoparticles (Yang et al. 2018a). However, it was observed that for Scenedesmus sp., coated magnetite NPs did not show better harvesting efficiency compared to uncoated nanoparticles, which is why cheap naked magnetite NPs can be used with advantage (Abo Markeb et al. 2019).
Magnetic flocculation of microalgal cells can be successfully combined with other procedures leading to target algal products; conversion of the Scenedesmus obliquus cells/particle slurry to biocrude oil using hydrothermal liquefaction process has been described. Zn-ferrite NPs enabled higher production of biocrude oil than naked magnetic iron oxide NPs (Egesa et al. 2018).
Regeneration of bound magnetic particles from the separated algal biomass is necessary to lower the cost of magnetic flocculants and the whole algae separation process. The regeneration process depends both on the magnetic flocculants and microalgae species. In many cases, ultrasonication in water or in organic solvents, treatment at high pH, or the combination of both procedures can be used successfully (Egesa et al. 2018; Xu et al. 2017, 2019; Abo Markeb et al. 2019). Alternatively, the bound iron oxide particles can be dissolved by strong acid and the released iron ions used for new magnetic particles synthesis (Duman et al. 2019).
A review of patented innovative harvesting processes for microalgal biomass production including magnetic flocculation techniques has been published recently (Deconinck et al. 2018). Despite the fact that a large number of studies have been published in this area, the application of magnetic particles needs to consider the complex impacts on the downstream processing of products production. Currently, such issues have not been addressed and need further investigation (Yin et al. 2020).
Magnetic separation and determination of algal biologically active compounds
Isolation and purification of biologically active compounds, including proteins, peptides, (poly)saccharides, oligonucleotides, nucleic acids, lipids, as well as of other specific molecules, are used in almost all branches of biosciences and biotechnologies. Isolation procedures, applicable also for work in complex solutions and suspensions, have become available for both small- and large-scale processes. Specific group of isolation techniques is based on the use of magnetically responsive materials, which can be applied for magnetic adsorption, affinity, ion exchange or hydrophobic batch separation, and applications of magnetically stabilized fluidized beds or magnetically modified two-phase systems (Safarik and Safarikova 2004; Franzreb et al. 2006).
Algae are very well known source of various valuable biologically active compounds (Hayes et al. 2019); on the contrary, selected algae can also produce important toxic compounds. There are many procedures describing isolation and separation of bioactive compounds from algae (Sosa-Hernández et al. 2018). Surprisingly, separations based on the use of magnetically responsive materials (Safarik and Safarikova 2004; Franzreb et al. 2006) have not found many applications in algae research and (bio)technology. Magnetic solid-phase extraction (MSPE), developed in 1999 by Safarikova and Safarik, has become a very useful tool for the preconcentration and separation of large amount of biologically active compounds, pollutants, heavy metal ions, etc. present in low concentrations (Safarikova and Safarik 1999; Jiang et al. 2019); this procedure has been efficiently used for the analysis of algal toxins (Li et al. 2017).
Fucoidan and laminarin, selected as typical brown algae water-soluble polysaccharides with interesting pharmaceutical properties, were separated from brown algae extract using magnetically responsive graphene oxide modified by four imidazole-based ionic liquids. Single-factor experiments showed that the extraction efficiency of polysaccharides was affected by the amount of ionic liquids for modification, solid–liquid ratio of brown alga and extraction agent (ethanol), the stirring time of brown alga and ionic liquid-modified magnetic graphene oxide materials, and amount of ionic liquid modified magnetic graphene oxide materials added to the brown alga sample solution. The results indicated that 1-(3-aminopropyl)imidazole chloride modified magnetic graphene oxide exhibited better extraction ability than graphene oxide, magnetic graphene oxide, and other three ionic liquid–modified magnetic graphene oxide materials. The highest extraction recoveries of fucoidan and laminarin extracted by 1-(3-aminopropyl)imidazole chloride modified magnetic graphene oxide were 93.3 and 87.2%, respectively; the adsorbent capacities were 100.2 mg g−1 for fucoidan and 38.6 mg g−1 for laminarin. In addition, solid materials could be separated and reused easily owing to their magnetic properties (Wang et al. 2017b).
Algal toxins are unique types of toxins generated with harmful algal blooms in water bodies. Algal toxins are hard to separate after they enter the water treatment processes, so an efficient procedure is required to remove the toxin from the water. A novel nanohybrid material, namely magnetic nanosilicate platelet (MNSP), was prepared and used for the removal of microcystin toxins. MNSP was fabricated by a special treatment of a clay mineral, montmorillonite, and then its surface was decorated with magnetite nanoparticles by in situ synthesis. The nanohybrid enabled efficient removal of microcystin-LR (99.39% at a concentration of 100 ppm), while pristine nanosilicate platelet could remove only 36.84% at the same dosage (Chang et al. 2017).
Magnetic separation of algal toxins can be negatively influenced by natural organic matter (NOM). During the study of adsorption of microcystin-LR on iron oxide NPs, it was observed that various types of NOM, namely extracellular polymeric substances from cyanobacteria and sodium salt of alginic acid from brown algae, efficiently stabilized the nanoparticles, but substantially decreased the toxin adsorption. The results indicated that NOM and toxin compete for limited adsorption sites (Yang et al. 2018b).
Microcystins produced by freshwater cyanobacteria can be efficiently determined in environmental water, using magnetic solid-phase extraction and high-performance liquid chromatography with UV detection. The magnetic composite material, which was combined with cetylpyridinium chloride, was prepared by hydrothermal synthesis. Under the optimal conditions, the limit of detection of microcystin-LR was 0.001 μg L−1 and the limit of quantification was 0.0028 μg L−1, while the limit of detection of microcystin-RR was 0.001 μg L−1 and the limit of quantification was 0.003 μg L−1. The magnetic solid-phase extraction adsorbent used in this method has the advantages of simple preparation, low price, and easy solid–liquid separation, and it can be used for the rapid and sensitive monitoring of trace microcystins in environmental water samples (Li et al. 2017).
Direct competitive enzyme-linked immunomagnetic colorimetric assays (ELIMC) have been developed for the determination of domoic acid, okadaic acid and saxitoxin in seawater. Magnetic beads with immobilized anti-rabbit IgG enabled immobilization of the specific rabbit IgG. Competition of the analyzed toxin with the toxin-horseradish peroxidase conjugate towards the bound specific antibody took place in a single and short step procedure, carried out into a microtube. The non-bound reagents were then discarded by capturing the immunomagnetic beads with a magnet, and after washing, the beads were suspended in peroxidase substrate solution. After a short incubation time, a stop solution was added and the activity of the captured enzyme was measured spectrophotometrically. In the presence of toxin, competition occurs and consequently, the rate of color production decreases proportionally to the concentration of the toxin (Petropoulos et al. 2019).
A similar approach was used for the determination of okadaic acid using epoxy group activated carbon shell magnetic beads with immobilized goat against mouse antibody. Mouse monoclonal antibody against okadaic acid, okadaic acid-horseradish peroxidase conjugate, and okadaic acid containing sample were simultaneously mixed with magnetic beads in a 96-well microtitration plate. After incubation, washing, and peroxidase substrate addition, the absorbance of the reaction mixture was measured (Pang et al. 2019).
Magnetic beads (NucleoMag Plant kit) were used for efficient, reliable, and fast DNA extraction of several macroalgae species. DNA extracted from macroalgae (Ulva spp.) using this method is of high quality and purity, allowing successful library preparation for next generation sequencing (Fort et al. 2018).
Algae-based production of magnetic iron oxide nanoparticles
Various procedures have been used to synthesize magnetic nano- and microparticles, such as classical coprecipitation, reactions in constrained environments (e.g., microemulsions), sol-gel syntheses, hydrolysis and thermolysis of precursors, sonochemical and microwave reactions, hydrothermal reactions, flow injection syntheses, electrospray syntheses, and mechanochemical processes (Laurent et al. 2008; Wu et al. 2015).
Biosynthesis of metal and metal oxide NPs using biological materials is currently of high interest. The nanobiotechnology synthesis of nanoparticles can be included into the group of clean manufacturing technologies. Clean technologies can significantly reduce environmental contamination and decrease the use of currently employed toxic chemicals and solvents, thus reducing the hazards to human health (Schröfel et al. 2014; Fawcett et al. 2017). The synthesis of nanoparticles can usually utilize plant tissues, plant extracts, exudates, and other parts of living and dead plants (Mahdavi et al. 2013).
Green biosynthesis of iron oxide nanoparticles employs the bottom-up approach where the iron atoms assemble to form clusters and then eventually the nanoparticles. The biological compounds present in green materials may act as both reducing and capping agents that can stabilize the nanoparticles during the synthesis process. Using this approach, it is possible to control the size and shape of the nanoparticles which can be used in various applications. Concentration of both metal salt and biological compounds, reaction time, as well as temperature and pH of the solution can be modified to obtain specific nanoparticles (Yew et al. 2020).
Recently, also marine macroalgae extracts have been successfully employed for the biosynthesis of magnetic iron oxide nanoparticles (Fawcett et al. 2017; Yew et al. 2020). Different types of brown, red, and green seaweed belonging to genera Sargassum, Colpomenia, Kappaphycus, Padina, Pterocladiella (Pterocladia), and Ulva have been used for this purpose (see Table 2 for more information). Usually, magnetite nanoparticles were synthesized by reduction of ferric chloride solution with seaweed water extract containing sulfated polysaccharides acting as both reducing agent and efficient stabilizer. In several papers, magnetite nanoparticles were biosynthesized using the mixture of Fe3+ and Fe2+ ions with a 2:1 molar ratio. The nanoparticles exhibited usually spherical or cubic shape with diameters ranging from 10 to 33 nm.
Biosynthesized iron oxide magnetic NPs have been applied for specific applications. Magnetite NPs prepared by the extracts of seaweeds Padina pavonica and Sargassum acinarium were entrapped in calcium alginates beads and used as magnetically responsive adsorbent for Pb2+ ion adsorption. Higher adsorption capacity was observed for alginate beads containing P. pavonica–synthesized magnetite (El-Kassas et al. 2016).
Ulva flexuosa–derived Fe3O4 NPs exhibited strong antibacterial action in in vitro tests against human pathogenic bacteria including Staphylococcus epidermidis, Bacillus subtilis and Bacillus pumilus, and moderate antifungal activity against Saccharomyces cerevisiae. The same NPs exhibited low acute toxicity against the rotifer Brachionus rotundiformis (Mashjoor et al. 2018). Similar results were obtained with magnetite NPs prepared with Ulva prolifera extract (Mashjoor et al. 2019).
Magnetite (Fe3O4) and cobalt ferrite (CoFe2O4) NPs were prepared by the coprecipitation method in aqueous solutions of a well-characterized agar obtained from the red marine alga Gelidium robustum. Highly crystalline-coated magnetite NPs were obtained; on the contrary, lowly crystalline cobalt ferrite NPs were formed. The NPs formed exhibited high degree of biocompatibility and can be considered as promising candidates for biomedical applications such as magnetic hyperthermia treatment for cancer therapy (Diaz-Blels et al. 2018).
Also, Chlorella vulgaris aqueous extract has been employed for algae-assisted auto-combustion method to prepare cobalt ferrite CoFe2O4 nanoparticles. After mixing Chlorella extract with cobalt nitrate and ferric nitrate solutions, urea solution was added to the mixture under mixing. After water evaporation by heating, the temperature was raised to 160 °C to achieve self-ignition; at this point, the dried gel was burnt in a self-propagating manner producing huge volume of gases and leaving behind dry and loose ferrite powder. The average particle size was 21.0 nm (Satheeshkumar et al. 2020).
Immobilized and modified algae cells
An absolute majority of prokaryotic and eukaryotic cells is diamagnetic. To add a response to external magnetic field, several modification procedures can be used such as the non-specific attachment of magnetic nanoparticles (e.g., by the magnetic fluid treatment), binding of maghemite or magnetite particles on the cell surface, covalent immobilization of magnetic particles on cell surface, immobilization of cells to magnetically responsive carriers, specific interactions with immunomagnetic nano- and microparticles, magnetic quantum dots or magnetoliposomes, biologically driven precipitation of paramagnetic compounds on the cell surface, and crosslinking of the cells or isolated cell walls with a bifunctional reagent in the presence of magnetic particles or entrapment (together with magnetic particles) into biocompatible polymers (Safarik et al. 2014).
Recently, C. vulgaris cells were modified with magnetite nanoparticles. The impact of various concentrations of magnetic nanoparticles on the microalgal cells growth and their metabolic status was investigated over 12 days. It was observed that high concentration of magnetic particles caused toxicity in microalgal cells damaging their organelles, mitochondria, and chloroplasts. After more than 6 days of exposure to stress generating concentrations of magnetic nanoparticles, it was found that microalgae could overcome the resulted damages. Therefore, in the aspect of the biotechnological process and environmental concerns caused by long-term exposure to magnetic nanoparticles, potential harmful toxic effects should only be expected during the initial days and at high concentrations (Taghizadeh et al. 2020).
A scalable and rapid method to prepare magnetically responsive agarose microbeads containing C. pyrenoidosa or other microbial cells was developed, using low-temperature melting agarose and food-grade sunflower oil as the main components for the emulsification process. The microscopic algae cells were immobilized in ∼ 100-μm-sized beads. Magnetically responsive microbeads were prepared by the immobilization of magnetically modified cells (using poly(allylamine)-stabilized magnetic nanoparticles) or by incorporation of magnetic calcium carbonate microcrystals. It was observed that the cells encapsulated in magnetically responsive microbeads were viable (Konnova and Fakhrullin 2017).
Magnetic algae–derived biosorbents for xenobiotics removal
Various types of organic and inorganic xenobiotics can be found in water systems all over the world. Several techniques for their removal have been developed; in many cases, biosorbents prepared from appropriate biological wastes have been used successfully. Magnetically responsive biosorbents enable their selective separation from the system using external magnetic field (Safarik et al. 2018).
Biomass from unicellular algae and marine macroalgae represents a typical example of low-cost, renewable natural material which can be obtained in large quantities. In many cases, huge amounts of marine algae can be found in beaches, thus causing problems to the tourist industry; the obtained biomass can be efficiently used as adsorbents for the removal of specific pollutants or for biochar production. In addition to native algae biomass, also waste biomass obtained after selected industrial processes (e.g., solvent extraction of oil or colorants) can be used for the preparation of efficient adsorbents. Special types of magnetic biosorbents have been prepared from microalgae and marine macroalgae; a review chapter summarizing this topic has been published recently (Safarik et al. 2020).
Magnetically modified algal biomass has been efficiently used for metal ion removal. Iron oxide particles and natural magnetic clay coated with polyethylenimine were successfully applied in the harvesting of Chlorella sp. Zinc ion removal efficiency of Chlorella cells was analyzed both before and after magnetic harvesting in order to determine the effect of magnetic particles on remediation efficiency. Native Chlorella cells exhibited quite high remediation efficiency; in addition, zinc-loaded Chlorella cells were perfectly harvested by both magnetic materials. Native Chlorella cells flocculated with polyethylenimine-coated magnetic clay exhibited high efficiency of zinc ion removal (Ferraro et al. 2018).
Chlorella vulgaris cells coated with magnetic iron oxide NPs were successfully employed in the removal of Cd(II) and Pb(II) from aqueous solutions. High percentage uptakes of these two toxic ions were observed in a wide range of pH and initial adsorbent concentrations. The material was found to be efficient in the rapid uptake of Cd(II)/Pb(II) from aqueous solutions. Simultaneous sorption experiments have shown that Cd(II) and Pb(II) were adsorbed at the different binding sites of the magnetic biosorbent; Cd(II) ions were bound with weak electrostatic forces to the dissociated carboxyl or hydroxyl groups, whereas Pb(II) ions were chemically bound with the amino groups (Lalhmunsiama et al. 2017).
Spirulina (Arthrospira) platensis modified with magnetite NPs and potassium nickel hexacyanoferrate was used as a biosorbent for uptake of Cs+; maximal adsorption capacity of 149 mg g−1 was reached. The cesium uptake was selective in the presence of Na+ and K+ ions. The used sorbent was easily separated by magnetic field and regenerated, keeping 85% of its initial capacity after five regeneration cycles (Azizkhani and Faghihian 2019).
Magnetically responsive algae-based biosorbents have also been applied for dye removal. The brown alga Sargassum horneri magnetically modified with microwave-synthesized iron oxide nano- and microparticles was used for adsorption of five water-soluble dyes of different chemical structures from aqueous solutions. The biosorption was studied in a batch system under different conditions. The adsorption equilibrium data were analyzed by the Langmuir and Freundlich isotherm models. The highest maximum adsorption capacity was observed for Acridine Orange (193.8 mg g−1) and the lowest one for Malachite Green (110.4 mg g−1). The sorption kinetics could be described by the pseudo-second-order model, and the thermodynamic studies indicated exothermic nature of biosorption process in the temperature range studied (Angelova et al. 2016).
Magnetic derivative of tropical marine green calcareous alga Cymopolia barbata was used as a biosorbent for the efficient Safranin O removal from aqueous solutions. Cymopolia barbata biomass was magnetically modified using microwave-synthesized magnetic iron oxide nano- and microparticles; this modification was simple and inexpensive, without the need of drying step. The Safranin biosorption was studied in a batch system under various conditions. Time necessary to reach equilibrium was 90 min. The adsorption isotherm data exhibited best correlation to the Freundlich and Langmuir adsorption models. The maximum adsorption capacity reached the value 192.2 mg g−1 (dry mass). Kinetic data were best fitted to the pseudo-second-order model. The adsorption process was exothermic and spontaneous (Mullerova et al. 2019).
Sargassum swartzii biomass modified with nanoscale zero-valent iron particles was employed for Crystal Violet adsorption from water solutions. Maximum biosorption capacity was observed at pH of 8. The Langmuir isotherm model enabled to calculate maximum adsorption capacity (200 mg g−1). Dye desorption was carried out with 0.1 M HCl (Jerold et al. 2017).
The brown alga Cystoseira barbata coated with magnetite particles was used for the removal of Methylene Blue from aqueous solution. The equilibrium data was analyzed with the Langmuir and Freundlich isotherms. The results showed that the maximum adsorption capacities were achieved at pH 2 and reached to 5.74 and 1.08 mg g−1 at 25 and 45 °C, respectively (Ozudogru et al. 2016).
The red unicell Porphyridium sp. has been modified by coating with Fe3O4 and silica NPs and utilized as a biosorbent for the removal of Methylene Blue. The adsorption followed the pseudo-second-order kinetic model and Freundlich adsorption isotherm. Maximum adsorption capacity was 96.93 mg g−1 at pH 6; this adsorbent can be used repeatedly (Buhani et al. 2019).
Biochar is a carbon-based material produced by pyrolysis of biomass in the absence of oxygen. In specific cases, algal biomass can be applied as biochar precursor. Laminaria (Saccharina) japonica–derived activated carbon/iron oxide magnetic composites were prepared by heating powdered biomass in nitrogen atmosphere; after cooling, the carbonized material was impregnated with ferric chloride and activated at 600–800 °C in nitrogen atmosphere. This biosorbent was used for adsorption of acetylsalicylic acid; the maximum adsorption capacity was ca 127 mg g−1 at 10 °C. The adsorption process followed the pseudo-second-order kinetic model and was controlled by physisorption and exothermic mechanisms (Jung et al. 2019).
Acid Orange 7 was separated by a magnetically modified brown marine macroalga biochar prepared by an electromagnetization technique. Magnetite-modified biochar exhibited high porosity which enabled high adsorption performance and easy magnetic separation from aqueous media. Maximum adsorption capacities were 190, 297, and 382 mg g−1 at 10, 20, and 30 °C, respectively (Jung et al. 2016).
Waste kelp and hijikia (a common seaweed found in South Korea that causes waste problems) were used to prepare magnetically modified biochar. Algae biomass was impregnated with FeCl3 solution and after drying this material was pyrolyzed at 500 °C under nitrogen. The prepared biochars demonstrated a high selectivity for copper (69.37 mg g−1 for kelp magnetic biochar and 63.52 mg g−1 for hijikia magnetic biochar), showing twofold greater removal efficiency than for zinc and cadmium (Son et al. 2018a).
Chitosan-modified magnetic kelp (brown algae) biochar was successfully synthesized for efficient removal of copper ions from wastewater. This chitosan containing composite exhibited 6 times higher surface area (6.17 m2 g−1) than the pristine magnetic kelp biochar (0.97 m2 g−1). The presence of new functional groups in chitosan-modified biochar improved the Cu2+ adsorption capacity. It was shown that the optimum pH value for the adsorption process was 6.9 (Son et al. 2018b).
The magnetite-modified activated carbon from Sargassum oligocystum was employed as a recyclable and efficient adsorbent for the removal of Methylene Blue and Methyl Violet from aqueous solutions. The adsorption process data matched well with the pseudo-second-order and Freundlich isotherm models. The maximum adsorption capacities were around 60 mg g−1 for both dyes (Foroutan et al. 2019).
The marine green alga Enteromorpha (Ulva) modified with Co(NO3)2 and (NH4)6Mo7O24 and subsequently carbonized at 700 °C was used as an efficient adsorbent for Methyl Blue removal. This carbon-based adsorbent contained highly dispersed CoOx/MoOy NPs on its surface and exhibited magnetic properties. High maximum adsorption capacity for Methyl Blue (1587.3 m2 g−1) was observed (Yang et al. 2019).
Harmful algal bloom biomass (blue–green algae) and ferric ammonium citrate or ferric nitrate were employed as precursors for preparing magnetic algal-based biochar. This material efficiently removed Cr(VI); the adsorption values were 165 m2 g−1 at pH 2 and 73 m2 g−1 at pH 6, with rapid kinetics. The composites maintained 73–82% of their removal capacity after five removal/recovery cycles (Cui et al. 2019).
Magnetic catalysts in algae biotechnology
Utilization of magnetically responsive materials and particles can bring a considerable simplification into algal-based biorefinery technologies, based on their fast, simple, and selective separation even from various difficult-to-handle environments and conditions. Magnetically responsive (bio)catalysts can be reused after their simple magnetic recovery, thus avoiding the filtration or centrifugation separation processes. Additionally, application of magnetic (bio)catalysts can significantly improve the productivity, economic feasibility, sustainability, and product quality during the algal-based biorefinery processes (Safarik et al. 2016a).
Magnetic catalyst based on Fe2O3 “core” particles was prepared by precipitation; subsequently, it was coated by porous silica and combined with clinoptilolite. This magnetic catalyst showed good performance for Chlorella hydrothermal liquefaction and derived biocrude upgrading. In 320–350 °C regime and under subcritical water, palmitic acid conversion was improved by 14–29% with the catalyst. Methyl palmitate conversion was 56% and decarboxylation selectivity to pentadecane was improved to 62% on the developed catalyst (Bian et al. 2017).
Magnetic NPs formed by Zn- and Mg-doped ferrite, used for Scenedesmus obliquus magnetic flocculation, were applied to convert the algal/particle slurry to biocrude oil using hydrothermal liquefaction. Liquefaction of algal/magnetic NPs slurry gave a biocrude oil yield of 37.1% while algae yielded only 23.2%. Hydrocarbon production in Zn-ferrite catalyzed and uncatalyzed biocrude oil formation were 46.5 and 19.9%, respectively, while the formations of heptadecane from Zn-ferrite catalyzed and uncatalyzed biocrude oil productions were 37.8 and 10%, respectively (Egesa et al. 2018).
Some reviews summarizing latest developments in microalgae-biofuel production with nanoadditives as catalysts, including magnetic ones, have been published recently (Hossain et al. 2019; Nematian and Barati 2019).
Conclusions
Magnetically responsive nano- and microparticles or their composites with diamagnetic materials have increasing potential for applications in many fields of biosciences, biotechnology, and environmental technology. These materials have also found interesting applications in algae biotechnology. Although such materials have been mainly used in small-scale (laboratory) applications, their ability to interact with external magnetic field predetermines their future applications also in large-scale biotechnology processes. Low cost, biocompatibility, high availability, and variability of magnetic materials and composites will enable their wide application in the near future.
References
Abo Markeb A, Llimós-Turet J, Ferrer I, Blanquez P, Alonso A, Sánchez A, Moral-Vico J, Font X (2019) The use of magnetic iron oxide based nanoparticles to improve microalgae harvesting in real wastewater. Water Res 159:490–500
Ali EMM, Elashkar AA, El-Kassas HY, Salim EI (2018) Methotrexate loaded on magnetite iron nanoparticles coated with chitosan: biosynthesis, characterization, and impact on human breast cancer MCF-7 cell line. Int J Biol Macromol 120:1170–1180
Angelova R, Baldikova E, Pospiskova K, Maderova Z, Safarikova M, Safarik I (2016) Magnetically modified Sargassum horneri biomass as an adsorbent for organic dye removal. J Clean Prod 137:189–194
Azizkhani M, Faghihian H (2019) Application of a novel adsorbent prepared using magnetized Spirulina platensis algae modified by potassium nickel hexacyanoferrate for removal of cesium, studied by response surface methodology. C R Chim 22:562–573
Bian JJ, Zhang Q, Zhang P, Feng LJ, Li CH (2017) Supported Fe2O3 nanoparticles for catalytic upgrading of microalgae hydrothermal liquefaction derived bio-oil. Catal Today 293:159–166
Boli E, Savvidou M, Logothetis D, Louli V, Pappa G, Voutsas E, Kolisis F, Magoulas K (2017) Magnetic harvesting of marine algae Nannochloropsis oceanica. Sep Sci Technol. https://doi.org/10.1080/01496395.2017.1296463
Budlayan MLM, Alguno AC, Capangpangan RY (2019) Influence of Sargassum crassifolium extract on the absorption of magnetic iron oxide nanoparticle via green synthesis route. Key Eng Mater 803:382–386
Buhani H, Suharso F, Rinawati, Sumadi (2019) Magnetized algae-silica hybrid from Porphyridium sp. biomass with Fe3O4 particle and its application as adsorbent for the removal of methylene blue from aqueous solution. Desalin Water Treat 142:331–340
Chang S-C, Lu B-L, Lin J-J, Li Y-H, Lee M-R (2017) A method to prepare magnetic nanosilicate platelets for effective removal of Microcystis aeruginosa and microcystin-LR. In: Holst O (ed) Microbial toxins: methods and protocols. Springer, New York, pp 85–94
Cui Y, He H, Atkinson JD (2019) Iron/carbon composites for Cr(VI) removal prepared from harmful algal bloom biomass via metal bioaccumulation or biosorption. ACS Sustain Chem Eng 7:1229–1288
Deconinck N, Muylaert K, Ivens W, Vandamme D (2018) Innovative harvesting processes for microalgae biomass production: a perspective from patent literature. Algal Res 31:469–477
Diaz-Blels D, Alvarado-Gil JJ, Martinez AI, Gomez-Y-Gomez Y, Freile-Pelegrin Y (2018) On the preparation and characterization of superparamagnetic nanoparticles with Gelidium robustum agar coating for biomedical applications. Bull Mater Sci 41:39
Duman F, Sahin U, Atabani AE (2019) Harvesting of blooming microalgae using green synthetized magnetic maghemite (γ-Fe2O3) nanoparticles for biofuel production. Fuel 256:115935
Egesa D, Chuck CJ, Plucinski P (2018) Multifunctional role of magnetic nanoparticles in efficient microalgae separation and catalytic hydrothermal liquefaction. ACS Sustain Chem Eng 6:991–999
El-Kassas HY, Aly-Eldeen MA, Gharib SM (2016) Green synthesis of iron oxide (Fe3O4) nanoparticles using two selected brown seaweeds: characterization and application for lead bioremediation. Acta Oceanol Sin 35:89–98
Fawcett D, Verduin JJ, Shah M, Sharma SB, Poinern GEJ (2017) A review of current research into the biogenic synthesis of metal and metal oxide nanoparticles via marine algae and seagrasses. J. Nanosci 2017:8013850
Ferraro G, Toranzo RM, Castiglioni DM, Lima E, Vasquez Mansilla M, Fellenz NA, Zysler RD, Pasquevich DM, Bagnato C (2018) Zinc removal by Chlorella sp. biomass and harvesting with low cost magnetic particles. Algal Res 33:266–276
Foroutan R, Mohammadi R, Razeghi J, Ramavandi B (2019) Performance of algal activated carbon/Fe3O4 magnetic composite for cationic dyes removal from aqueous solutions. Algal Res 40:101509
Fort A, Guiry MD, Sulpice R (2018) Magnetic beads, a particularly effective novel method for extraction of NGS-ready DNA from macroalgae. Algal Res 32:308–313
Fraga-Garcia P, Kubbutat P, Brammen M, Schwaminger S, Berensmeier S (2018) Bare iron oxide nanoparticles for magnetic harvesting of microalgae: from interaction behavior to process realization. Nanomaterials 8:292
Franzreb M, Siemann-Herzberg M, Hobley TJ, Thomas ORT (2006) Protein purification using magnetic adsorbent particles. Appl Microbiol Biotechnol 70:505–516
Gerulová K, Bartošová A, Blinová L, Bártová K, Dománková M, Garaiová Z, Palcut M (2018) Magnetic Fe3O4-polyethyleneimine nanocomposites for efficient harvesting of Chlorella zofingiensis, Chlorella vulgaris, Chlorella sorokiniana, Chlorella ellipsoidea and Botryococcus braunii. Algal Res 33:165–172
Hayes M, Bastiaens L, Gouveia L, Gkelis S, Skomedal H, Skjanes K, Murray P, García-Vaquero M, Hosoglu MI, Dodd J, Konstantinou D, Safarik I, Zittelli GC, Rimkus V, Pino V, Muylaert K, Edwards C, Laake M, Silva JG, Pereira H, Abelho J (2019) Microalgal bioactive compounds including protein, peptides, and pigments: applications, opportunities, and challenges during biorefinery processes. In: Hayes M (ed) Novel proteins for food, pharmaceuticals and agriculture. John Wiley and Sons, Ltd., London, pp 239–255
Hena S, Fatihah N, Tabassum S, Lalung J, Jing SY (2016) Magnetophoretic harvesting of freshwater microalgae using polypyrrole/Fe3O4 nanocomposite and its reusability. J Appl Phycol 28:1597–1609
Hossain N, Mahlia TMI, Saidur R (2019) Latest development in microalgae-biofuel production with nano-additives. Biotechnol Biofuels 12:125
Jerold M, Vasantharaj K, Joseph D, Sivasubramanian V (2017) Fabrication of hybrid biosorbent nanoscale zero-valent iron-Sargassum swartzii biocomposite for the removal of crystal violet from aqueous solution. Int J Phytoremediation 19:214–224
Jiang H-L, Li N, Cui L, Wang X, Zhao R-S (2019) Recent application of magnetic solid phase extraction for food safety analysis. TrAC Trends Anal Chem 120:115632
Jung K-W, Choi BH, Jeong T-U, Ahn K-H (2016) Facile synthesis of magnetic biochar/Fe3O4 nanocomposites using electro-magnetization technique and its application on the removal of acid orange 7 from aqueous media. Bioresour Technol 220:672–676
Jung K-W, Choi BH, Song KG, Choi J-W (2019) Statistical optimization of preparing marine macroalgae derived activated carbon/iron oxide magnetic composites for sequestering acetylsalicylic acid from aqueous media using response surface methodologys. Chemosphere 215:432–443
Konnova S, Fakhrullin R (2017) Fabrication of magnetically responsive agarose microbeads doped with live microbial cells. Bionanoscience 7:75–77
Kuang L, Goins J, Zheng W, Eduafo P, Ma H, Posewitz M, Wu DT, Liang H (2019) Spontaneous microalgae dewatering directed by retrievable, recyclable, and reusable nanoparticle-pinched polymer brushes. Chem Mater 31:4657–4672
Lalhmunsiama, Gupta PL, Jung H, Tiwari D, Kong S-H, Lee S-M (2017) Insight into the mechanism of Cd(II) and Pb(II) removal by sustainable magnetic biosorbent precursor to Chlorella vulgaris. J Taiwan Inst Chem Eng 71:206–213
Laurent S, Forge D, Port M, Roch A, Robic C, Elst LV, Muller RN (2008) Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications. Chem Rev 108:2064–2110
Li Q, Lian L, Wang X, Wang R, Tian Y, Guo X, Lou D (2017) Analysis of microcystins using high-performance liquid chromatography and magnetic solid-phase extraction with silica-coated magnetite with cetylpyridinium chloride. J Sep Sci 40:1644–1650
Liu P-R, Wang T, Yang Z-Y, Hong Y, Hou Y-L (2017) Long-chain poly-arginine functionalized porous Fe3O4 microspheres as magnetic flocculant for efficient harvesting of oleaginous microalgae. Algal Res 27:99–108
Liu P-R, Yang Z-Y, Hong Y, Hou Y-L (2018) An in situ method for synthesis of magnetic nanomaterials and efficient harvesting for oleaginous microalgae in algal culture. Algal Res 31:173–182
Liu Y, Jin W, Zhou X, Han S-F, Tu R, Feng X, Jensen PD, Wang Q (2019) Efficient harvesting of Chlorella pyrenoidosa and Scenedesmus obliquus cultivated in urban sewage by magnetic flocculation using nano-Fe3O4 coated with polyethyleneimine. Bioresour Technol 290:121771
Ma J, Xia W, Fu X, Ding L, Kong Y, Zhang H, Fu K (2020) Magnetic flocculation of algae-laden raw water and removal of extracellular organic matter by using composite flocculant of Fe3O4/cationic polyacrylamide. J Clean Prod 248:119276
Mahdavi M, Namvar F, Bin Ahmad M, Mohamad R (2013) Green biosynthesis and characterization of magnetic iron oxide (Fe3O4) nanoparticles using seaweed (Sargassum muticum) aqueous extract. Molecules 18:5954–5964
Mashjoor S, Yousefzadi M, Zolgharnain H, Kamrani E, Alishahi M (2018) Organic and inorganic nano-Fe3O4: alga Ulva flexuosa-based synthesis, antimicrobial effects and acute toxicity to briny water rotifer Brachionus rotundiformis. Environ Pollut 237:50–64
Mashjoor S, Yousefzadi M, Zolgharnein H, Kamrani E, Alishahi M (2019) Phyco-linked vs chemogenic magnetite nanoparticles: route selectivity in nano-synthesis, antibacterial and acute zooplanktonic responses. Mater Sci Eng C 102:324–340
Matter IA, Bui VKH, Jung M, Seo JY, Kim Y-E, Lee Y-C, Oh Y-K (2019) Flocculation harvesting techniques for microalgae: a review. Appl Sci 9:3069
Milledge JJ, Heaven S (2013) A review of the harvesting of micro-algae for biofuel production. Rev Environ Sci Biotechnol 12:165–178
Mullerova S, Baldikova E, Prochazkova J, Pospiskova K, Safarik I (2019) Magnetically modified macroalgae Cymopolia barbata biomass as an adsorbent for safranin O removal. Mater Chem Phys 225:174–180
Nematian T, Barati M (2019) Nanobiocatalytic processes for producing biodiesel from algae. In: Rai M, Ingle AP (eds) Sustainable bioenergy. Elsevier, Amsterdam, pp 299–326
Ozudogru Y, Merdivan M, Göksan T (2016) Biosorption of methylene blue from aqueous solutions by iron oxide-coated Cystoseira barbata. J Turk Chem Soc A 3:551–564
Pang L, Quan H, Sun Y, Wang P, Ma D, Mu P, Chai T, Zhang Y, Hammock BD (2019) A rapid competitive ELISA assay of okadaic acid level based on epoxy-functionalized magnetic beads. Food Agric Immunol 30:1286–1302
Petropoulos K, Bodini SF, Fabiani L, Micheli L, Porchetta A, Piermarini S, Volpe G, Pasquazzi FM, Sanfilippo L, Moscetta P, Chiavarini S, Palleschi G (2019) Re-modeling ELISA kits embedded in an automated system suitable for on-line detection of algal toxins in seawater. Sens Actuators B 283:865–872
Safarik I, Baldikova E, Prochazkova J, Safarikova M, Pospiskova K (2018) Magnetically modified agricultural and food waste: preparation and application. J Agric Food Chem 66:2538–2552
Safarik I, Maderova Z, Pospiskova K, Horska K, Safarikova M (2014) Magnetic decoration and labeling of prokaryotic and eukaryotic cells. In: Fakhrullin RF, Choi I, Lvov YM (eds) Cell surface engineering: fabrication of functional nanoshells. RSC Smart Materials No. 9. RSC, London, pp 185–215
Safarik I, Pospiskova K, Baldikova E, Safarikova M (2016a) Development of advanced biorefinery concepts using magnetically responsive materials. Biochem Eng J 116:17–26
Safarik I, Pospiskova K, Baldikova E, Safarikova M (2017) Magnetic particles for microalgae separation and biotechnology. In: Puri M (ed) Food bioactives: extraction and biotechnology applications. Springer, Cham, pp 153–169
Safarik I, Pospiskova K, Horska K, Safarikova M (2012) Potential of magnetically responsive (nano)biocomposites. Soft Matter 8:5407–5413
Safarik I, Prochazkova G, Pospiskova K, Branyik T (2016b) Magnetically modified microalgae and their applications. Crit Rev Biotechnol 36:931–941
Safarik I, Prochazkova J, Baldikova E, Pospiskova K (2020) Magnetically responsive algae and seagrass derivatives for pollutant removal. In: Vilarinho C, Castro F, Goncalves M, Fernando AL (eds) Wastes: solutions, treatments and opportunities III. CRC Press, Boca Raton, pp 131–136
Safarik I, Safarikova M (2004) Magnetic techniques for the isolation and purification of proteins and peptides. BioMagn Res Technol 2:7
Safarikova M, Safarik I (1999) Magnetic solid-phase extraction. J Magn Magn Mater 194:108–112
Salem DMSA, Ismail MM, Aly-Eldeen MA (2019) Biogenic synthesis and antimicrobial potency of iron oxide (Fe3O4) nanoparticles using algae harvested from the Mediterranean Sea. Egypt J Aquat Res 45:197–204
Satheeshkumar MK, Kumar ER, Indhumathi P, Srinivas C, Deepty M, Sathiyaraj S, Suriyanarayanan N, Sastry DL (2020) Structural, morphological and magnetic properties of algae/CoFe2O4 and algae/Ag-Fe-O nanocomposites and their biomedical applications. Inorg Chem Commun 111:107578
Seo JY, Kim MG, Lee K, Lee Y-C, Na J-G, Jeon SG, Park SB, Oh Y-K (2017) Multifunctional nanoparticle applications to microalgal biorefinery. In: Rai M, da Silva SS (eds) Nanotechnology for bioenergy and biofuel production. Springer, Cham, pp 59–87
Seo JY, Jeon HJ, Kim JW, Lee J, Oh YK, Ahn CW, Lee JW (2018) Simulated-sunlight-driven cell lysis of magnetophoretically separated microalgae using ZnFe2O4 octahedrons. Ind Eng Chem Res 57:1655–1661
Schröfel A, Kratošová G, Šafařík I, Šafaříková M, Raška I, Shor LM (2014) Applications of biosynthesized metallic nanoparticles – a review. Acta Biomater 10:4023–4042
Son E-B, Poo K-M, Chang J-S, Chae K-J (2018a) Heavy metal removal from aqueous solutions using engineered magnetic biochars derived from waste marine macro-algal biomass. Sci Total Environ 615:161–168
Son E-B, Poo K-M, Mohamed HO, Choi Y-J, Cho W-C, Chae K-J (2018b) A novel approach to developing a reusable marine macro-algae adsorbent with chitosan and ferric oxide for simultaneous efficient heavy metal removal and easy magnetic separation. Bioresour Technol 259:381–387
Sosa-Hernández JE, Escobedo-Avellaneda Z, Iqbal HMN, Welti-Chanes J (2018) State-of-the-art extraction methodologies for bioactive compounds from algal biome to meet bio-economy challenges and opportunities. Molecules 23:2953
Taghizadeh S-M, Berenjian A, Chew KW, Show PL, Zaid HFM, Ramezani H, Ghasemi Y, Raee MJ, Ebrahiminezhad A (2020) Impact of magnetic immobilization on the cell physiology of green unicellular algae Chlorella vulgaris. Bioengineered 11:141–153
Vashist V, Chauhan D, Bhattacharya A, Rai MP (2019) Role of silica coated magnetic nanoparticle on cell flocculation, lipid extraction and linoleic acid production from Chlorella pyrenoidosa. Nat Prod Res in press
Vergini S, Aravantinou AF, Manariotis ID (2016) Harvesting of freshwater and marine microalgae by common flocculants and magnetic microparticles. J Appl Phycol 28:1041–1049
Wang B, Wu D, Chu KH, Ye LQ, Yip HY, Cai ZH, Wong PK (2017a) Removal of harmful alga, Chattonella marina, by recyclable natural magnetic sphalerite. J Hazard Mater 324:498–506
Wang XQ, Li GZ, Row KH (2017b) Magnetic graphene oxide modified by imidazole-based ionic liquids for the magnetic-based solid-phase extraction of polysaccharides from brown alga. J Sep Sci 40:3301–3310
Wang XY, Zhao Y, Jiang XX, Liu LJ, Li X, Li HX, Liang WY (2018) In-situ self-assembly of plant polyphenol-coated Fe3O4 particles for oleaginous microalgae harvesting. J Environ Manag 214:335–345
Wu W, Wu ZH, Yu T, Jiang CZ, Kim WS (2015) Recent progress on magnetic iron oxide nanoparticles: synthesis, surface functional strategies and biomedical applications. Sci Technol Adv Mater 16:023501
Xu Y, Wang X, Fu Y, Hu F, Qian G, Liu Q, Sun Y (2019) Interaction energy and detachment of magnetic nanoparticles-algae. Environ Technol in press
Xu YF, Fu Y, Zhang DY (2017) Cost-effectiveness analysis on magnetic harvesting of algal cells. Mater Today Proc 4:50–56
Yang L, Wang Y, Liu A, Zhang Y (2019) CoOx/MoOy-anchored multi-wrinkled biomass carbon as a promising material for rapidly selective methyl blue removal. J Mater Sci 54:11024–11036
Yang Y, Hou J, Wang P, Wang C, Miao L, Ao Y, Xu Y, Wang X, Lv B, You G, Yang Z (2018a) Interpretation of the disparity in harvesting efficiency of different types of Microcystis aeruginosa using polyethylenimine (PEI)-coated magnetic nanoparticles. Algal Res 29:257–265
Yang YY, Hou J, Wang PF, Wang C, Miao LZ, Ao YH, Wang X, Lv BW, You GX, Liu ZL, Shao YX (2018b) The effects of extracellular polymeric substances on magnetic iron oxide nanoparticles stability and the removal of microcystin-LR in aqueous environments. Ecotoxicol Environ Saf 148:89–96
Yew YP, Shameli K, Miyake M, Kuwano N, Bt Ahmad Khairudin NB, Bt Mohamad SE, Lee KX (2016) Green synthesis of magnetite (Fe3O4) nanoparticles using seaweed (Kappaphycus alvarezii) extract. Nanoscale Res Lett 11:276
Yew YP, Shameli K, Miyake M, Ahmad Khairudin NBB, Mohamad SEB, Naiki T, Lee KX (2020) Green biosynthesis of superparamagnetic magnetite Fe3O4 nanoparticles and biomedical applications in targeted anticancer drug delivery system: a review. Arab J Chem 13:2287–2308
Yin Z, Zhu L, Li S, Hu T, Chu R, Mo F, Hu D, Liu C, Li B (2020) A comprehensive review on cultivation and harvesting of microalgae for biodiesel production: environmental pollution control and future directions. Bioresour Technol 301:122804
Zhu LD, Hiltunen E, Li Z (2019) Using magnetic materials to harvest microalgal biomass: evaluation of harvesting and detachment efficiency. Environ Technol 40:1006–1012
Acknowledgments
This work was carried out in the frame of the COST Actions ES1408 “European network for algal-bioproducts (EUALGAE)” and CA18238 “European transdisciplinary networking platform for marine biotechnology.”
Funding
The research was supported by the project LTC17020 (Ministry of Education, Youth and Sports of the Czech Republic) and by ERDF project “Development of pre-applied research in nanotechnology and biotechnology” (No. CZ.02.1.01/0.0/0.0/17_048/0007323).
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Safarik, I., Baldikova, E., Prochazkova, J. et al. Magnetic particles in algae biotechnology: recent updates. J Appl Phycol 32, 1743–1753 (2020). https://doi.org/10.1007/s10811-020-02109-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10811-020-02109-0