Abstract
Field experiments were conducted to study the impact of metal accumulation on malondialdehyde (MDA), cysteine and non-protein thiol (NPSH) contents in the plants of Prosopis juliflora grown on the fly ash (FA) amended with soil, blue green algae (BGA) biofertilizer, farm yard manure, press mud and Rhizobium inoculation. The analysis of data revealed that the level of MDA, cysteine and NPSH was higher in the roots of the plant than leaves, which was found positively correlated with metal accumulation. An increase of 361.14, 64.25 and 305.62% in MDA, cysteine and NPSH contents, respectively was observed after 45 days in the roots of the plants grown in 100% FA as compared to 100% garden soil (GS). The level of MDA, cysteine and NPSH was found less in the plants grown on various amendments of FA showing ameliorating effect on the toxicity induced due to the accumulation of metals. The decrease in MDA, cysteine and NPSH contents was higher in Rhizobium-inoculated plants as compared to uninoculated plants grown on 100% FA. The results showed a high tolerance potential of the plant, which is further increased by inoculating the plant with FA-tolerant Rhizobium showing feasibility of using P. juliflora in environmental monitoring of FA landfills.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Cheung, K.C., Wong, J.P.K., Zhang, Z.Q., Wong, J.W.C. and Wong, M.H.: 2000, ‘Revegetation of lagoon ash using the legume sp. Acacia auriculiformis and Leucaena leucocephala’, Environ. Pollut. 109, 75–82.
De Vos, C.H.R., Vooijs, R., Schat, H. and Ernst, W.H. O.: 1959, ‘Copper induced damage to permeability barrier in roots of Silene cucubalus’, Plant Physiol. 135, 165–169.
Ellman, G.L.: 1959, ‘Tissue sulfhydryl groups’, Arch. Biochem. Biophys. 82, 70–77.
Gaitonde, M.K.: 1957, ‘A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids’, Biochem. J. 104, 627–633.
Galli, U., Scheupp, H. and Breenold, C.: 1996, ‘Thiols in cadmium and copper treated maize (Zea mays L.)’, Planta. 198, 139–143.
Gomez, K.A. and Gomez, A.A.: 1984, Statistical Procedures for Agricultural Research, Wiley, New York.
Grill, E., Winnacker, E.L. and Zenk, M.H.: 1987, ‘Phytochelatins, a class of heavy metal-binding peptides from plants, are functionally analogous to metallothioneins’, Proc. Natl. Acad. Sci. U.S.A. 84, 439–443.
Halliwell, B. and Gutteridge, J.M.C.: 1993, Free Radicals in Biology and Medicine, Clarendon Press, Oxford.
Heath, R.L. and Packer, L.: 1968, ‘Photoperoxidation in isolated chloroplasts: I. Kinetic and stoichiometry of fatty acid peroxidation’, Arch. Biochem. Biophys. 125, 189–198.
Jensen, H.L.: 1942, ‘Nitrogen Fixation in Leguminous Plants: I. General Characters of Root Nodule Bacteria Isolated from Species of Medicago and Trifolium’, in Proceedings of Australian Linnean Society, New South Wales, Vol. 66, pp. 98–108.
Khan, M.R. and Khan, M.W.: 1996, ‘The effect of fly ash on plant growth and yield of tomato’, Environ. Pollut. 92, 105–111.
Kumar, A., Vajpayee, P., Ali, M.B., Tripathi, R.D., Singh, N., Rai, U.N. and Singh, S.N.: 2002, ‘Biochemical responses of Cassia siamea Lamk. grown on coal combustion residue (Fly-ash)’, Bull. Environ. Contam. Toxicol. 68, 675–683.
Mazhoudi, S., Chaoui, A., Ghorbal, M. and Ferjani, E.E.: 1997, ‘Response of antioxidant enzymes to excess copper in tomato (Lycopersicon esculentum, Mill)’, Plant Sci. 127, 129–137.
Mehra, A., Farago, M.E. and Banerjee, D.K.: 1998, ‘Impact of fly ash from coal fired stations in Delhi, with particular reference to metal contamination’, Environ. Monit. Assess. 50, 15–35.
Mulhern, D.W., Robel, R.J., Furness, J.C. and Hensley, D.L.: 1989, ‘Vegetation of waste disposal areas at a coal find power plants on fly ash’, Environ. Sci. Technol. 13, 311–315.
Nussbaum, S., Schmutz, D. and Brunold, C.: 1988, ‘Regulation of assimilatory sulfate reduction by cadmium in Zea mays L.’, Plant Physiol. 88, 1407–1410.
Rai, U.N., Tripathi, R.D., Gupta, M. and Chandra, P.: 1995, ‘Induction of phytochelatins and cadmium stress in water lettuce (Pistia stratiotes)’, J. Environ. Sci. Health A30(9), 2007–2026.
Rai, U.N., Pandey, K., Sinha, S., Singh, A., Saxena, R. and Gupta, D.K.: 2004, ‘Revegetating fly-ash land fills with Prosopis juliflora L.: Impacts of different amendments and Rhizobium inoculation’, Environ. Int. 30, 293–300.
Rauser, W.E.: 1987, ‘Copper binding protein and copper tolerance in Agrostis gigantea’, Plant Sci. Lett. 33, 239–247.
Sinha, S., Gupta, M. and Chandra, P.: 1997, ‘Oxidative stress induced by iron in Hydrilla verticillata (l.f.) Royle: Response of antioxidants’, Ecotoxicol. Environ. Safety 38, 286–291.
Somashekaraiah, B.V., Padmaja, K. and Prasad, A.R.K.: 1992, ‘Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorophyll degradation’, Physiol. Planta 85, 85–89.
Vajpayee, P., Rai, U.N., Choudhary, S.K., Tripathi, R.D. and Singh, S. N.: 2000, ‘Management of fly ash landfills with Cassia surattensis Burm: A case study’, Bull. Environ. Contam. Toxicol. 65, 675–682.
Vincent, J.M.: 1970, A Manual for the Practical Study of the Root Nodule Bacteria. International Biological Programme Handbook,vol. 15, Blackwell, Oxford.
Wong, J.W.C. and Wong, M.H.: 1990, ‘Effects of fly ash on yields and elemental composition of two vegetables, Brassica parachinensis and B. chinensis’, Agric. Ecosys. Environ. 30, 254–264.
Zenk, M.H.: 1996, ‘Heavy metal detoxification in higher plants — A review’, Gene 179, 21–30.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sinha, S., Rai, U.N., Bhatt, K. et al. Fly-ash-induced oxidative stress and tolerance in Prosopis juliflora L. grown on different amended substrates. Environ Monit Assess 102, 447–457 (2005). https://doi.org/10.1007/s10661-005-6397-4
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10661-005-6397-4