Abstract
The aim of this research was to determine whether exogenous abscisic acid (ABA) applied immediately after ex vitro transfer of in vitro grown plants can improve their acclimatization. Tobacco (Nicotiana tabacum L.) plantlets were transferred into pots with Perlite initially moistened either by water or 50 µM ABA solution and they were grown under low (LI) or high (HI) irradiance of 150 and 700 µmol m−2 s−1, respectively. Endogenous content of ABA in tobacco leaves increased considerably after ABA application and even more in plants grown under HI. Stomatal conductance, transpiration rate and net photosynthetic rate decreased considerably 1 d after ex vitro transfer and increased thereafter. The gas exchange parameters were further decreased by ABA application and so wilting of these plants was limited. Chlorophyll (a+b) and β-carotene contents were higher in ABA-treated plants, but the content of xanthophyll cycle pigments was not increased. However, the degree of xanthophyll cycle pigments deepoxidation was decreased what also suggested less stress in ABA-treated plants. No dramatic changes in most chlorophyll a fluorescence parameters after ex vitro transfer suggested that the plants did not suffer from restriction of electron transport or photosystem damage.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- ABA:
-
abscisic acid
- Car:
-
carotenoids
- Chl:
-
chlorophyll
- DEPS:
-
degreee of XCP deepoxidation
- Fm :
-
maximum chlorophyll fluorescence
- Fv :
-
variable chlorophyll fluorescence
- PS:
-
photosystem
- qNP :
-
non-photochemical quenching
- qP :
-
photochemical quenching
- RWC:
-
relative water content
- WUE:
-
water use efficiency
- XCP:
-
xanthophyll cycle pigments
- ΦPS2 :
-
quantum yield of PS 2 photochemistry
References
Agarwal, S., Sairam, R.K., Srivastava, G.C., Meena, R.C.: Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes.-Biol. Plant. 49: 541–550, 2005.
Aguilar, M.L., Espadas, F.L., Coello, J., Maust, B.E., Trejo, C., Robert, M.L., Santamaría, J.M.: The role of abscisic acid in controlling leaf water loss, survival and growth of micropropagated Tagetes erecta plants when transferred directly to the field.-J. exp. Bot. 51: 1861–1866, 2000.
Amâncio, S., Rebordão, J.P., Chaves, M.M.: Improvement of acclimatization of micropropagated grapevine: photosynthetic competence and carbon allocation.-Plant Cell Tissue Organ Cult. 58: 31–37, 1999.
Baroja, M.E., Aguirreolea, J., Sánchez-Díaz, M.: CO2 exchange of in vitro and acclimatizated potato plantlets.-In: Carre, F., Chagvardieff, P. (ed.): Ecophysiology and Photosynthetic in Vitro Cultures. Pp. 187–188. CEA, Saint-Paul-lez-Durance 1995.
Bieleski, R.L.: The problem of halting enzyme action when extracting plant tissues.-Anal. Biochem. 9: 431–442, 1964.
Bhatia, P., Ashwath, N.: Comparative performance of micropropagated and seed-grown tomato plants.-Biol. Plant. 48: 625–628, 2004.
Bray, E.A.: Abscisic acid regulation of gene expression during water-deficit stress in the era of the Arabidopsis genome.-Plant Cell Environ. 25: 153–161, 2002.
Carvalho, L.C., Osório, M.L., Chaves, M.M., Amâncio, S.: Chlorophyll fluorescence as an indicator of photosynthetic functioning of in vitro grapevine and chestnut plantlets under ex vitro acclimatization.-Plant Cell Tissue Organ Cult. 67: 271–280, 2001.
Čatský, J.: Determination of water deficit in disks cut out from leaf blades.-Biol. Plant. 2: 76–78, 1960.
Colón-Guasp, W., Nell, T.A., Kane, M.E., Barrett, J.E.: Effects of abscisic acid on ex vitro acclimatization of Aronia arbutifolia (L.) Pers.-J. amer. Soc. hort. Sci. 121: 101–104, 1996.
Díaz-Pérez, J.C., Sutter, E.G., Shackel, K.A.: Acclimatization and subsequent gas exchange, water relations, survival and growth of microcultured apple plantlets after transplanting them in soil.-Physiol. Plant. 95: 225–232, 1995.
Estrada-Luna, A.A., Davies, F.T., Jr., Egilla, J.N.: Physiological changes and growth of micropropagated chile ancho pepper plantlets during acclimatization and post-acclimatization.-Plant Cell Tissue Organ Cult. 66: 17–24, 2001.
Fila, G., Badeck, F.-W., Meyer, S., Cerovic, Z., Ghashghaie, J.: Relationship between leaf conductance to CO2 diffusion and photosynthesis in micropropagated grapevine plants, before and after ex vitro acclimatization.-J. exp. Bot. 57: 2687–2695, 2006.
Fila, G., Ghashghaie, J., Hoarau, J., Cornic, G.: Photosynthesis, leaf conductance and water relations of in vitro cultured grapevine rootstock in relation to acclimatisation.-Physiol. Plant. 102: 411–418, 1998.
Fuentes, G., Talavera, C., Desjardins, Y., Santamaria, J.M.: High irradiance can minimize the negative effect of exogenous sucrose on the photosynthetic capacity of in vitro grown coconut plantlets.-Biol. Plant. 49: 7–15, 2005.
Genoud, C., Coudret, A., Amalric, C., Sallanon, H.: Effect of micropropagation conditions of rose shootlets on chlorophyll fluorescence.-Photosynthetica 36: 243–251, 1999.
Giraudat, J., Parcy, F., Bertauche, N., Gost, F., Leung, J., Morris, P.-C., Bouvier-Durand, M., Vartanian, N.: Current advances in abscisic acid action and signalling.-Plant mol. Biol. 26: 1557–1577, 1994.
Guan, Q.Z., Guo, Y.H., Siu, X.L., Li, W., Zhang, Z.X.: Changes in photosynthetic capacity and antioxidant enzymatic systems in micropropagated Zingiber officinale plantlets during their acclimation.-Photosynthetica 46: 193–201, 2008.
Haisel, D., Pospíšilová, J., Synková, H., Čatský, J., Wilhelmová, N., Plzáková, Š.: Photosynthetic pigments and gas exchange of in vitro grown tobacco plants as affected by CO2 supply.-Biol. Plant. 42: 463–468, 1999.
Haisel, D., Pospíšilová, J., Synková, H., Schnablová, R., Bat’ková, P.: Effects of abscisic acid or benzyladenine on pigment contents, chlorophyll fluorescence, and chloroplast ultrastructure during water stress and after rehydration.-Photosynthetica 44: 606–614, 2006.
Haisel, D., Vaňková, R., Synková, H., Pospíšilová, J.: The impact of trans-zeatin O-glucosyltransferase gene over-expression in tobacco on pigment content and gas exchange.-Biol. Plant. 52: 49–58, 2008.
Hartung, W., Abou-Mandour, A.A.: A beneficial role of abscisic acid for regenerates of Ruta graveolens ssp. divaricata (Tenore) Gams suffering from transplant shock.-Angew. Bot. 70: 221–223, 1996.
Hofman, P., Haisel, D., Komenda, J., Vágner, M., Tichá, I., Schäfer, C., Čapková, V.: Impact of in vitro cultivation conditions on stress responses and on changes in thylakoid membrane proteins and pigments of tobacco during ex vitro acclimation.-Biol. Plant. 45: 189–195, 2002.
Hronková, M., Zahradníčková, H., Šimková, M., Šimek, P., Heydová, A.: The role of abscisic acid in acclimation of plants cultivated in vitro to ex vitro conditions.-Biol. Plant. 46: 535–541, 2003.
Ivanov, A.G., Krol, M., Maxwell, D., Huner, N.P.A.: Abscisic acid induced protection against photoinhibition of PSII correlates with enhanced activity of the xanthophyll cycle.-FEBS Lett. 371: 61–64, 1995.
Jeon, M.-W., Ali, M.B., Hahn, E.-J., Paek, K.-Y.: Photosynthetic pigments, morphology and leaf gas exchange during ex vitro acclimatization of micropropagated CAM Doritaenopsis plantlets under relative humidity and air temperature.-Environ. exp. Bot. 55: 183–194, 2006.
Jia, H., Lu, C.: Effects of abscisic acid on photoinhibition in maize plants.-Plant Sci. 165: 1403–1410, 2003.
Kadleček, P., Rank, B., Tichá, I.: Photosynthesis and photoprotection in Nicotiana tabacum L. in vitro-grown plantlets.-J. Plant Physiol. 160: 1017–1024, 2003.
Kadleček, P., Tichá, I., Čapková, V., Schäfer, C.: Acclimatization of micropropagated tobacco plantlets.-In: Garab, G. (ed.): Photosynthesis: Mechanisms and Effects. Vol. V. Pp. 3853–3856. Kluwer Academic Publishers, Dordrecht 1998.
Kadleček, P., Tichá, I., Haisel, D., Čapková, V., Schäfer, C.: Importance of in vitro pretreatment for ex vitro acclimatization and growth.-Plant Sci. 161: 695–701, 2001.
Lamhamedi, M.S., Chamberland, H., Tremblay, F.M.: Epidermal transpiration, ultrastructural characteristics and net photosynthesis of white spruce somatic seedlings in response to in vitro acclimatization.-Physiol. Plant. 118: 554–561, 2003.
Majada, J.P., Luz Centeno, M., Feito, I., Fernándéz, B., Sanchez-Tames, R.: Stomatal and cuticular traits on carnation tissue culture under different ventilation conditions.-Plant Growth Regul. 25: 113–121, 1998.
Mizrahi, Y., Scherings, S.G., Arad, S.M., Richmond, A.E.: Aspects of the effects of ABA on the water status of barley and wheat sedlings.-Physiol. Plant. 31: 44–50, 1974.
Osório, M.L., Gonçalves, S., Osório, J., Romano, A.: Effects of CO2 concentration on acclimatization and physiological responses of two cultivars of carob tree.-Biol. Plant. 49: 161–167, 2005.
Pandey, D.M., Yu, K.W., Wu, R.Z., Hahn, E.-J., Paek, K.-Y.: Effects of different irradiances on the photosynthetic process during ex-vitro acclimation of Anoectochilus plantlets.-Photosynthetica 44: 419–424, 2006.
Pospíšilová, J.: Hardening by abscisic acid of tobacco plantlets grown in vitro.-Biol. Plant. 38: 605–609, 1996.
Pospíšilová, J., Bat’ková, P.: Effects of pre-treatments with abscisic acid and/or benzyladenine on gas exchange of French bean, sugar beet, and maize leaves during water stress and after rehydration.-Biol. Plant. 48: 395–399, 2004.
Pospíšilová, J., Haisel, D., Synková, H., Čatský, J., Wilhelmová, N., Plzáková, Š., Procházková, D.: Photosynthetic pigments and gas exchange of in vitro grown tobacco plants during ex vitro acclimation.-Plant Cell Tissue Organ Cult. 61: 125–133, 2000.
Pospíšilová, J., Synková, H., Haisel, D., Čatský, J., Wilhelmová, N., Šrámek, F.: Effect of elevated CO2 concentration on acclimation of tobacco plantlets to ex vitro conditions.-J. exp. Bot. 50: 119–126, 1999.
Pospíšilová, J., Synková, H., Haisel, D., Semorádová, Š.: Acclimation of plantlets to ex vitro conditions: effects of air humidity, irradiance, CO2 concentration and abscisic acid.-Acta Hort. 748: 29–38, 2007.
Pospíšilová, J., Wilhelmová, N., Synková, H., Čatský, J., Krebs, D., Tichá, I., Hanáčková, B., Snopek, J.: Acclimation of tobacco plantlets to ex vitro conditions as affected by application of abscisic acid.-J. exp. Bot. 49: 863–869, 1998.
Rival, A., Beulé, T., Lavergne, D., Nato, A., Havaux, M., Puard, M.: Development of photosynthetic characteristics in oil palm during in vitro micropropagation.-J. Plant Physiol. 150: 520–527, 1997.
Sallanon, H., Berger, M., Genoud, C., Coudret, A.: Water stress and photoinhibition in acclimatization of Rosa hybrida plantlets.-In Vitro cell. dev. Biol. Plant 34: 169–172, 1998.
Semorádová, Š., Synková, H., Pospíšilová, J.: Responses of tobacco plantlets to change of irradiance during transfer from in vitro to ex vitro conditions.-Photosynthetica 40: 605–614, 2002.
Seon, J.-H., Cui, Y.-Y., Kozai, T., Paek, K.-Y.: Influence of in vitro growth conditions on photosynthetic competence and survival rate of Rehmania glutinosa plantlets during acclimatization period.-Plant Cell Tissue Organ Cult. 61: 135–142, 2000.
Serret, M.D., Trillas, M.I., Araus, J.L.: The effect of in vitro culture conditions on the pattern of photoinhibition during acclimation of gardenia plantlets to ex vitro conditions.-Photosynthetica 39: 67–73, 2001a.
Serret, M.D., Trillas, M.I., Matas, J., Araus, J.L.: The effect of photoautotrophy on photosynthesis and photoinhibition of gardenia plantlets during micropropagation.-Photosynthetica 39: 245–255, 2001b.
Siddique, I., Anis, M.: An improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L.-Acta physiol. Plant. 30: 493–499, 2008.
Slavtcheva, T., Dimitrova, V.: Gas exchange of in vitro and ex vitro grown grapevine plants.-Photosynthetica 39: 29–33, 2001.
Stuchlíková, K., Hejnák, V., Šafránková, I.: The effect of abscisic acid and benzylaminopurine on photosynthesis and transpiration rates of maize (Zea mays L.) under water stress and subsequent rehydration.-Cereal Res. Commun. 35: 1593–1602, 2007.
Synková, H.: Sucrose affects the photosynthetic apparatus and the acclimation of transgenic tobacco to ex vitro culture.-Photosynthetica 33: 403–412, 1997.
Tichá, I., Radochová, B., Kadleček, P.: Stomatal morphology during acclimatization of tobacco plantlets to ex vitro conditions.-Biol. Plant. 42: 469–474, 1999.
Trillas, M.I., Serret, M.D., Jorba, J., Araus, J.L.: Leaf chlorophyll fluorescence changes during acclimatization of the root-stock GF677 (peach × almond) and propagation of Gardenia jasminoïdes E.-In: Carre, F., Chagvardieff, P. (ed.): Ecophysiology and Photosynthetic in vitro Cultures. Pp. 161–168. CEA, Saint-Paul-lez-Durance 1995.
Van Huylenbroeck, J.M.: Influence of light stress during the acclimation of in vitro plantlets.-In: Struik, P.C., Vredenberg, W.J., Renkema, J.A., Parlevliet, J.E. (ed.): Plant Production on the Threshold of a New Century. Pp. 451–453. Kluwer Academic Publishers, Dordrecht 1994.
Van Huylenbroeck, J.M., Debergh, P.C.: Impact of sugar concentration in vitro on photosynthesis and carbon metabolism during ex vitro acclimatization of Spathiphyllum plantlets.-Physiol. Plant. 96: 298–304, 1996.
Van Huylenbroeck, J.M., Huygens, H., Debergh, P.C.: Photoinhibition during acclimatization of micropropagated Spathiphyllum “Petite” plantlets.-In Vitro cell. dev. Biol. Plant 31: 160–164, 1995.
Van Huylenbroeck, J.M., Piqueras, A., Debergh, P.C.: Photosynthesis and carbon metabolism in leaves formed prior and during ex vitro acclimatization of micropropagated plants.-Plant. Sci. 134: 21–30, 1998.
Van Huylenbroeck, J.M., Piqueras, A., Debergh, P.C.: The evolution of photosynthetic capacity and the antioxidant enzymatic system during acclimatization of micropropagated Calathea plants.-Plant Sci. 155: 59–66, 2000.
Van Kooten, O., Snel, J.F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology.-Photosynth. Res. 25: 147–150, 1990.
Yang, S.-H., Yeh, D.M.: In vitro leaf anatomy, ex vitro photosynthetic behaviors and growth of Calathea orbifolia (Linden) Kennedy plants obtained from semi-solid and temporary immersion systems.-Plant Cell Tissue Organ Cult. 93: 201–207, 2008.
Zhou, Y.-H., Guo, D.-P., Zhu, Z.-J., Qian, Q.-Q.: Effect of in vitro rooting environments and irradiance on growth and photosynthesis of strawberry plantlets during acclimatization.-Plant Cell Tissue Organ Cult. 81: 105–108, 2005.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Pospíšilová, J., Synková, H., Haisel, D. et al. Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets. Biol Plant 53, 11–20 (2009). https://doi.org/10.1007/s10535-009-0003-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10535-009-0003-5