Biofilms formed by Pseudomonas aeruginosa strains isolated from biomaterial of patients with implant-associated infection are characterized by much higher resistance to antibiotics of various classes than plankton cultures of these strains. The concentrations of antibiotics causing the death of 90% of P. aeruginosa biofilm (MIC90) was 2-6 μg/ml for fluoroquinolones, 267-356 μg/ml for cephalosporins, and 92-215 μg/ml for amikacin, which significantly (p<0.05) differed from MIC90 for plankton cultures that did not exceed 0.8 μg/ml for fluoroquinolones, 19 μg/ml for cephalosporins, and 3 μg/ml for amikacin. The degree of the microbial biofilm maturity also affected antibiotic resistance.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Babushkina IV, Mamonova IA, Ulyanov VY, Shpinyak SP, Bondarenko AS. Effects of Ciprofloxacin on the Dynamics of Biofilm Formation by Staphylococcus Epidermidis Strains Isolated from Implant-Associated Infection. Vestn. Eksper. Klin. Khir. 2020;13(3):241-247. doi: https://doi.org/10.18499/2070-478X-2020-13-3-241-247. Russian.
Babushkina IV, Ulyanov VY, Mamonova IA, Shpinyak SP. The Effect of Azithromycin on Biofilms Formation by Pathogens of Implant-Associated Infection in Large Joints. Bull. Exp. Biol. Med. 2020;169(6):798-801. doi: https://doi.org/10.1007/s10517-020-04982-8
Borodulin VB, Durnova NA, Vasiliadis RA, Losev OE, Chesovskih YS, Goroshinskaya IA, Kachesova PS, Babushkina IV, Polozhentsev OE. Study of the biological effect of iron nanoparticles. Nanotechnologies in Russia. 2015;10(3-4):268-277.
Murylev V, Kukovenko G, Elizarov P, Rukin Ya, Tslgln N. Periprosthetic infection during hip arthroplasty. Vrach. 2018; 29(3):17-22. doi: https://doi.org/10.29296/25877305-2018-03-04. Russian.
Babushkina IV, Bondarenko AS, Ulyanov VY, Mamonova IA. Biofilm formation by gram-negative bacteria during implantassociated infection. Bull. Exp. Biol. Med. 2020;169(3):365-368. doi: https://doi.org/10.1007/s10517-020-04888-5
Babushkina IV, Gladkova EV, Belova SV, Norkin IA. Application of preparations containing copper nanoparticles for the treatment of experimental septic wounds. Bull. Exp. Biol. Med. 2017;164(2):162-164. doi: https://doi.org/10.1007/s10517-017-3948-y
Chatterjee M, D’Morris S, Paul V, Warrier S, Vasudevan AK, Vanuopadath M, Nair SS, Paul-Prasanth B, Mohan CG, Biswas R. Mechanistic understanding of Phenyllactic acid mediated inhibition of quorum sensing and biofilm development in Pseudomonas aeruginosa. Appl. Microbiol. Biotechnol. 2017;101(22):8223-8236. doi: https://doi.org/10.1007/s00253-017-8546-4
Guitor AK, Wright GD. Antimicrobial resistance and respiratory infections. Chest. 2018;154(5):1202-1212. doi: https://doi.org/10.1016/j.chest.2018.06.019
Li S, Chen S, Fan J, Cao Z, Ouyang W, Tong N, Hu X, Hu J, Li P, Feng Z, Huang X, Li Y, Xie M, He R, Jian J, Wu B, Xu C, Wu W, Guo J, Lin J, Sun P. Anti-biofilm effect of novel thiazole acid analogs against Pseudomonas aeruginosa through IQS pathways. Eur. J. Med. Chem. 2018;145:64-73. doi: https://doi.org/10.1016/j.ejmech.2017.12.076
Müh U, Schuster M, Heim R, Singh A, Olson ER, Greenberg EP. Novel Pseudomonas aeruginosa quorum-sensing inhibitors identified in an ultra-high-throughput screen. Antimicrob. Agents Chemother. 2006;50(11):3674-3679. doi: https://doi.org/10.1128/AAC.00665-06
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 8, pp. 182-185, August, 2021
Rights and permissions
About this article
Cite this article
Babushkina, I.V., Mamonova, I.А., Ulyanov, V.Y. et al. Resistance to Antibiotics in Plankton and Biofilm Cultures of Pseudomonas aeruginosa Clinical Strains. Bull Exp Biol Med 172, 155–157 (2021). https://doi.org/10.1007/s10517-021-05354-6
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-021-05354-6