Abstract
This study aimed to improve the antioxidant and nitric oxide scavenging activities of ginseng marc fermented by Pediococcus acidilactici, thereby creating a biofunctional resource with improved anti-inflammatory capability. P. acidilactici was inoculated in 1% ginseng marc extract; cell viability, pH, and total titratable acidity were measured. Total phenolic and flavonoid contents were measured using Folin–Ciocalteu reagent and colorimetric method. Ferric reducing antioxidant power (FRAP), β-carotene, and sodium nitroprusside (SNP) assays were used to evaluate functionality. Polyphenols and flavonoids totaled 33.7 ± 0.4 and 10.0 ± 0.4 mg/g of solid, respectively, at 24 h fermentation. P. acidilactici had 40 nM β-galactosidase and 20 nM β-glucosidase activities. Antioxidative activities increased up to 34.5 and 10.2%, respectively, as measured via FRAP and β-carotene assays. Anti-inflammatory activity of the fermented extract—as measured via SNP assay—increased 342%, suggesting that ginseng marc fermented by P. acidilactici could be used in food or pharmaceutical industries.
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Introduction
Korean ginseng, Panax ginseng Meyer, is widely consumed as a functional food because it has a good safety profile and exhibits pharmaceutical effects. As the demand for ginseng has increased, the amount of ginseng marc has also increased [1]. Ginseng marc, a byproduct of the extraction of ginseng, contains biologically active compounds such as polyphenols and ginsenoside, but ginseng marc has traditionally been discarded [2]. Various polyphenols such as gallic acid, protocatechuic acid, vanillic acid, caffeic acid, coumaric acid, kaempferol, and quercetin were found in ginseng marc. Both in vitro and in vivo experiments have shown that these natural polyphenols and flavonoids exhibit antioxidant and anti-inflammatory properties [3]. However, many bioconversion techniques have recently been used to increase biofunctional activities by using fungi or bacteria such as lactic acid bacteria (LAB) [4].
LAB derived from traditional foods are undergoing studies to screen for probiotic properties or to study their application to raw materials for fermentation [5]. Among LAB, Pediococcus acidilactici is found in fermented vegetables, in meat, and in dairy products. P. acidilactici is known as a probiotic that has shown promising results in animal and human experiments [6]. Some LAB have known to produce β-glucosidase during fermentation [7]. The β-glucosidase enzymes are important factors in ginseng fermentation because these enzymes are capable of decomposing glycoside to a aglycone and sugars [8]. Increases in glucosidase or galactosidase result in increases in the quantities of minor ginsenosides. Accordingly, functionality related to polyphenols is enhanced [9].
Fermented milk is widely consumed all over the world because of its nutritional quality and sensory properties [10, 11]. Milk products are fortified with minerals, vitamins, or herbal extracts [12, 13] to improve functional properties. In recent decades, several study of probiotic fermented milk have concentrated on assessment of anti-inflammatory properties [14], allergic processes [15], and modulating the immune response [16].
During the process of inflammation, NO, pro-inflammatory, and prostaglandin were produced by inducible NO synthase and cyclooxygenase-2 (COX-2) [17]. In particular, several compounds such as NO, iron, and reactive oxygen species (ROS) are recognized as inducing factors of human diseases such as Alzheimer’s, Parkinson’s, chronic kidney disease, coronary heart disease, insulin-dependent diabetes mellitus (IDDM), myocardial infarction, and mutagenic activity [18]. Moreover, NO has been known to play a key role in the central nervous, cardiovascular, and immune systems [12].
Therefore, the aim of this study was to evaluate whether fermenting ginseng marc with P. acidilactici increases the antioxidative and anti-inflammatory activities such that it has practical application as a functional food or in the pharmaceutical industry.
Materials and methods
Microorganism
Pediococcus acidilactici was isolated from kimchi and identified as KCCM 11614P by the Korean Culture Center of Microorganisms (Seoul, Korea). The strain was cultured and activated in Man Rogosa Sharpe (MRS) broth at 37 °C for 24 h.
Chemicals
MRS broth was purchased from Difco Laboratories (Detroit, MI, USA). Aluminum nitrate; ammonium thiocyanate; β-carotene; ferric chloride; kaempferol; linoleic acid; phosphoric acid; potassium acetate; potassium ferricyanide; quercetin; (SNP); sulfanilamide; 2,4,6-tripyridyl-s-triazine (TPTZ); and Tween 80 were purchased from Sigma–Aldrich Company (St. Louis, MO, USA). Acetonitrile, chloroform, ethanol (95%), and methanol were obtained from J.T. Baker (Austin, TX, USA).
Preparation of ginseng marc fermentation
Panax ginseng marc was obtained from Ginseng Research Institute of Il Hwa Co., Ltd., (Guri-si, Gyeonggi-do, Korea). Ginseng marc was extracted three times with 70% ethanol and filtered with a 0.45-μm membrane filter. Ethanol extract were concentrated a vacuum evaporator (EYELA N-1000 V, Tokyo, Japan). Crude extracts were freeze dried and stored at −20 °C until used.
An initial cell number was adjusted to approximately 6.3 log colony-forming units (CFU)/mL. The fermentation medium comprised 4 g ginseng marc and 400 mL distilled water. During fermentation, cultured samples were taken at 0, 8, 16, and 24 h for various tests. To estimate the viable cell number, 100 μL of serially diluted fermented extract was plated on MRS agar plates, and single colonies were counted. TTA beyond pH 8.2 was determined via titration with 0.1 M sodium hydroxide (NaOH). TTA was calculated as the percentage of lactic acid as follows [19]:
Determination of total phenolics and flavonoids contents
The total phenolics content (TPC) was determined by the Folin–Ciocalteu method [19]. Each fermented extract (100 μL) was mixed with 2% sodium carbonate (2 mL) for 5 min and Folin–Ciocalteu’s reagent was then added. The mixture was allowed to stand at 25 °C for 30 min. Absorbencies were then measured at 750 nm with a spectrophotometer (Optizen, Mecasys, Korea), and TPC was expressed as gallic acid equivalents (mg GAE/g of solid).
The total flavonoid content (TFC) was determined by the colorimetric aluminum chloride method [19]. A 0.5 mL quantity of fermented extract was sequentially mixed with 1.5 mL of 95% ethanol, 0.1 mL of 10% aluminum nitrate in 1 M potassium acetate, and 2.8 mL of distilled water. The absorbance of the mixture after incubation at 25 °C for 30 min was measured at 415 nm. TFC was expressed as kaempferol equivalents (mg kaempferol/g of solid).
Enzymatic activities
The enzymatic activity of P. acidilactici KCCM 11614P was evaluated using the API ZYM kit (Biomerieux Inc., Marcy l’Etoile, France). The subcultured strains of 107 CFU/mL of strain were suspended in sterile saline (0.85% NaCl) and inoculated in each cupule at 37 °C for 4 h. ZYM-A and ZYM-B reagents were then added and the solutions observed for color change. Enzyme activities produced by the P. acidilactici strain during culturing were expressed as 0, 5, 10, 20, or 40 nM, as indicated in the API ZYM kit [20].
Determination of antioxidative activity
Ferric reducing antioxidant power assay
The FRAP of the fermented extract was determined by the method of Xiao et al. [21]. The FRAP reagent contained 2.5 mL TPTZ, 10 mM in 40 mM HCl, 2.5 mL iron chloride (FeCl3, 20 mM), and 25 mL acetate buffer (300 mM, pH 3.6). A 100 μL sample was added to 1.9 mL FRAP reagent at 37 °C for 30 min, and the absorbance was measured spectrophotometrically at 593 nm. Ferrous sulfate (FeSO2) was used for the standard curve.
β-Carotene/linoleic acid bleaching assay
The method of Terpinc et al. [22] was used to determine the antioxidant activity using β-carotene/linoleic acid. A 10 mL β-carotene reagent in chloroform (0.2 mg/mL) was mixed with 44 μL linoleic acid and 200 μL Tween 80. After evaporation of the reagent, 200 mL distilled water was added. A 4.5 mL aliquot of emulsion was added to the 0.5 mL sample and was measured at 470 nm. This reaction was performed at 50 °C for a 2 h interval. The β-carotene/linoleic acid bleaching was calculated as follows:
where Acontrol and Asample were absorbance of control and sample, respectively.
Ferric thiocyanate assay
The antioxidant activity was determined by the method of Su et al. using ferrous reducing power [23]. A 100 μL of sample was mixed with 200 μL distilled water, 200 μL linoleic acid, and 400 μL of potassium phosphate buffer (40 mM, pH 7). The mixture was incubated at 37 °C in the dark and absorbance at 500 nm was measured at 24 h intervals. The inhibition of lipid oxidation was calculated as follows:
where Acontrol and Asample were absorbance of control and sample, respectively.
Preparation of fermented milk
A ginseng marc extract (1%, w/v), skimmed milk powder (4%), and pectin (0.1%, w/v) were mixed in city milk (Seoul Milk Co., Seoul, Korea), and pasteurized at 85 °C for 10 min. After cooling, 1% P. acidilactici KCCM 11614P (initial cell number: 6.3 log CFU/mL) was inoculated into ginseng marc milk base at 40 °C in a water bath for 24 h.
NO scavenging activity
The NO scavenging activity was determined by the SNP assay [24]. A 10 mM SNP and sample were incubated at 37 °C for 24 h. We than added 100 μL Griess reagent to 100 μL reaction mixture, and measured absorbance at 540 nm. The NO scavenging activity was calculated as follows:
where Nc is the nitrite concentration of the control, and Ns the nitrite concentration of the sample.
Statistical analysis
All experiments were carried out in triplicate, and standard deviation values are presented. Linear regression analysis was used for total phenolic and flavonoid contents. One-way analysis of variance (ANOVA) was used to determine significant differences between samples in SNP assay using SPSS Statistics version 19 (SPSS Inc., Chicago, IL, USA).
Results and discussion
Effect of fermentation on viable cell, pH, and TTA
A viable cell number of ginseng marc extract fermented with P. acidilactici for 12 h increased from 6.3 to 7.5 log CFU/mL for 24 h (Fig. 1A). The pH of the fermented ginseng marc broth decreased from 6.5 to 4.8, and TTA increased from 0.25 to 0.42% (Fig. 1B). The TTA of milk supplemented with red ginseng extract increased from 0.10 ± 0.01 to 0.22 ± 0.01%, which can be attributed to factors in the media that promote bioconversion [11]. For example, in the case of fermented ginseng wine, studies have shown that lactic acid content increased up to 8% with LAB-induced fermentation [20].
Effect of fermentation on total polyphenol and flavonoid content
TPC and TFC of fermented ginseng marc extract as a function of fermentation time are shown in Table 1. The regression equations of total phenol and flavonoid contents are as follows (x = absorbance of sample):
Compared with the control (not fermented) ginseng marc extract, the fermented ginseng marc extract had higher TPC and TFC. The TPC of fermented ginseng marc extract increased to 25.4 to 30.0%, and the highest TPC was 33.7 ± 0.4 mg GAE/g of solid at 24 h of fermentation. Park et al. [19] found that the TPC of magnolia increased to 11.2 to 14.9% using P. acidilactici fermentation and fermented extract showed antioxidant and anticancer effect. Additionally, the TPC of ginseng seed [7] increased to 27.7 to 39.2% when fermented with Pediococcus pentosaceus.
Kaempferol is a standard substance used to measure flavonoid content because it is known to exist in ginseng root and leaves [25]. This study showed that flavonoids such as the kaempferol in ginseng marc extract might be slightly increased to 11.5 to 34.4% during fermentation.
Enzymatic activity of P. acidilactici KCCM 11614P
The API ZYM kit is used for detection of 20 types of enzymatic activity. The enzyme β-glucosidase is among those that decomposes phenolic glycoside to phenolic acid [4]. Since phenolic compounds have a positive correlation with antioxidants [24, 26], fermentation by microorganisms producing β-glucosidase could improve antioxidant activity.
The β-glucosidase activity of P. acidilactici was 20 nM of hydrolyzed substrate (Table 2). Other researchers reported that P. acidilactici isolated from inziangsang, a traditional fermented vegetable product in India, had 20 nM of β-glucosidase activity, and P. pentosaceus isolated from traditional fermented rice noodles had β-glucosidase activity of 30 nM [27]. Tamang et al. [20] found that P. pentosaceus isolated from gundruk, which is a lactic-fermented vegetable product processed manly in Nepal, referred to positive reaction of β-glucosidase. The β-galactosidase displayed a minimum high activity at 40 nM in P. acidilactici. Kim [28] showed β-galactosidase producing microorganisms was able to used for ginseng bioconversion. Theriault et al. [29] reported that phenolic compound from maple syrup with β-galactosidase showed antioxidant, antiradical, and antimutagenic properties. However, β-glucuronidase, which is one of the factors in developing colorectal cancer, was not detected in this study [30].
Effect of fermented ginseng marc on antioxidant activity
FRAP assays were performed to evaluate the reducing power of the ferric-tripyridyltriazine complex to a ferrous-tripyridyltriazine complex. The FRAP values of fermented ginseng marc extract increased from 362.3 ± 4.3 to 487.3 ± 13.2 mM FeSO4 eq (Table 3), as β-glucosidase breaks glycoside down to phenolic compounds. Researchers have evaluated the antioxidative activities of American, Asian, and Siberian ginseng roots through FRAP activity [31]. Moreover, the FRAP value was reported to increase over control both in red ginseng marc fermented by Bacillus subtilis [32] and in chickpeas fermented by Cordyceps militaris [21].
β-Carotene/linoleic acid bleaching assays were performed to evaluate the inhibition of lipid oxidation. Inhibition of β-carotene oxidation of fermented ginseng marc increased from 59.6 ± 0.1 to 65.7 ± 0.8% (Table 3). Lee et al. [33] reported that the leaf, stem, and root of Panax ginseng exhibited inhibition of linoleic acid oxidation. Park et al. [34] showed that kaempferol (flavonols with 3-hydroxyl) had high lipoxygenase inhibition activity rather than glycosidic substituents and showed scavenging activity of oxygen species by terminating radical chaing reactions.
FTC assays were performed to evaluate the inhibition of lipid peroxidation using ammonium thiocyanate. The antioxidant activity of fermented ginseng extract using the FRAP and β-carotene bleaching assays was higher than that for the non-fermented ginseng marc extract. In the FTC assay, the antioxidant activity of non-fermentation ginseng marc extract was slightly higher than that of the fermented samples (Table 3). During fermentation, TFC having inhibition activity of peroxidation might be decreased. Researchers have reported that the TPC of rabbit-eye blueberry juice prior to fermentation was higher than that of the fermented skin, whereas the antioxidant activity of juice prior to fermentation—as determined by FTC assay—was lower than that of fermented skin [23].
The effect of fermented milk added ginseng marc extract on NO scavenging activity
Milk was prepared to examine the applicability of ginseng marc and P. acidilactici on food industry and the amount of NO was measured to confirm functionality of fermented milk added ginseng marc extract. NO plays a role in inflammation because it increases vascular permeability and induces toxic oxidative responses [3]. The NO generated from SNP reacts with superoxide (O2 −) to form ONOO−, which are known to induce inflammation [24]. Therefore, we performed the SNP assay to evaluate NO concentration. The NO scavenging of non-fermented and fermented milk added ginseng marc extract for 24 h was 6.4 and 28.3%, respectively (Fig. 2). Phenolic acid and flavonoid were able to remove reactive nitrogen intermediates and increase phenolics levels in ginseng marc through LAB fermentation [35]. Several studies showed that plant foods such as ginseng, grape, and longan contain high levels of phenolics and flavonoids, which inhibit NO synthesis in both in vitro and in vivo experiments [36]. In particular, kaempferol, a major flavonoid compound in ginseng, has been reported to suppress proinflammatory cytokines (interleukin-6 [IL-6], IL-8, interleukin-1 receptor-associated kinase [IRAK1], and IRAK4) [15, 37].
This study aimed to enhance the antioxidant and anti-inflammatory activities of ginseng marc using fermentation. During a 24 h fermentation, P. acidilactici was successfully grown in ginseng marc extract. TPC and TFC of the fermented ginseng marc increased, and β-glucosidase activity was detected in P. acidilactici KCCM 11614P. Our results demonstrate that enhanced antioxidant and anti-inflammatory activities of the fermented ginseng marc resulted in increased production of phenolic compounds by β-glucosidase produced by P. acidilactici KCCM 11614P. Ginseng marc as by product in ginseng processing has relatively low bioactivity but this study suggests that using fermentation to improve the antioxidant and NO scavenging activities of ginseng marc is a viable method for increasing its use as a biofunctional resource in the food and pharmaceutical industries. In addition, P. acidilactici KCCM 11614P reveal that fermentability of herb or other by-products from agriculture and applicability to dairy products as an additional starter.
References
Kim HG, Jang SS, Lee JS, Kim HS, Son CG. Panax ginseng Meyer prevents radiation-induced liver injury via modulation of oxidative stress and apoptosis. J. Ginseng Res. 41:159–168 (2016)
Lee SD, Yoo G, Chae HJ, In MJ, Oh NS, Hwang YK, Hwang WI, Kim DC. Lipid-soluble extracts as the main source of anticancer activity in ginseng and ginseng marc. J. Am. Oil Chem. Soc. 86:1065–1071 (2009)
Kunworarath N, Rangkadilok N, Suriyo T, Thiantanawat A, Satayavivad J. Longan (Dimocarpus longan Lour.) inhibits lipopolysaccharide-stimulated nitric oxide production in macrophages by suppressing NF-κB and AP-1 signaling pathways. J. Ethnopharmacol. 179:156–161 (2016)
Zheng Z, Shetty K. Solid-state bioconversion of phenolics from cranberry pomace and role of Lentinus edodes β-glucosidase. J. Agric. Food Chem. 48:895–900 (2000)
Saraoui T, Cornet J, Guillouet E, Pilet MF, Chevalier F, Joffraud J, Leroi F. Improving simultaneously the quality and safety of cooked and peeled shrimp using a cocktail of bioprotective lactic acid bacteria. Int. J. Food Microbiol. 241:69–77 (2017)
Barros R. Phenotypic and genotypic characterization of Pediococcus strains isolated from human clinical sources. J. Clinical Microbiol. 39:1241–1246 (2001)
Lee MH, Lee YC, Kim SS, Hong HD, Kim KT. Quality and antioxidant activity of ginseng seed processed by fermentation strains. J. Ginseng Res. 39:178–182 (2015)
Lee BH, You HJ, Park MS, Kwon B, Ji GE. Transformation of the glycosides from food materials by probiotics and food microorganisms. J. Microbiol. Biotechnol. 16:497–504 (2006)
Ramos LR, Santos JS, Daguer H, Valese AC, Cruz AG, Granto D. Analytical optimization of a phenolic-rich herbal extract and supplementation in fermented milk containing sweet potato pulp. Food Chem. 221:950–958 (2016)
Eichler K, Wieser S, Rüthemann I, Brügger U. Effects of micronutrient fortified milk and cereal food for infants and children: a systematic review. BMC Publ. Health 12:506–518 (2012)
Jung JE, Yoon HJ, Yu HS, Lee NK, Jee HS, Paik HD. Short communication: Physicochemical and antioxidant properties of milk supplemented with red ginseng extract. J. Dairy Sci. 98:95–99 (2015)
Kawahara M, Nemoto M, Nakata T, Kondo S, Takahashi H, Kimura B, Kuda T. Anti-inflammatory properties of fermented soy milk with Lactococcus lactis subsp. lactis S-SU2 in murine macrophage RAW264.7 cells and DSS-induced IBD model mice. Int. Immunopharmacol. 26:295–303 (2015)
Velez EMM, Galdeano CM, Carmuega E, Weill R, Bonet MEB, Perdigon G. Probiotic fermented milk consumption modulates the allergic process induced by ovoalbumin in mice. Br. J. Nutr. 114:566–576 (2015)
Santiago-López L, Hernández-Mendoza A, Garcia HS, Mata-Haro V, Vallejo-Cordoba B, Gonzalez-Cordova AF. The effects of consuming probiotic-fermented milk on the immune system: A review of scientific evidence. Int. J. Dairy Technol. 68:153–165. (2015)
Kim SH, Park JG, Lee J, Yang WS, Park GW, Kim HG, Yi Y, Baek KS, Sung NK, Hossen MJ, Lee M, Kim J, Cho JY. The dietary flavonoid kaempferol mediates anti-inflammatory responses via the Src, Syk, IRAK1, and IRAK4 molecular targets. Mediat. Inflamm. 2015:904142 (2015)
Valko M, Jomova K, Rhodes CJ, Kuca K, Musilek K. Redox- and non-redox-metal-induced formation of free radicals and their role in human disease. Arch. Toxicol. 90:1–37 (2016)
Serafini M, Del Rio D. Understanding the association between dietary antioxidants, redox status and disease: is the total antioxidant capacity the right tool? Redox Report 9:145–152 (2004)
Lundberg JO, Gladwin MT, Weitzberg E. Strategies to increase nitric oxide signalling in cardiovascular disease. Nat. Rev. Drug Discovery 14:623–641 (2015)
Park EH, Kim HS, Eom SJ, Kim KT, Paik HD. Antioxidative and anticanceric activities of Magnolia (Magnolia denudata) flower petal extract fermented by Pediococcus acidilactici KCCM 11614. Molecules 20:12154–12165 (2015)
Tamang JP, Tamang B, Schillinger U, Guigas C, Holzapfel WH. Functional properties of lactic acid bacteria isolated from ethnic fermented vegetables of the Himalayas. Int. J. Food Microbiol. 135:28–33 (2009)
Xiao Y, Xing G, Rui X, Li Wei, Chen X, Jiang M, Dong M. Enhancement of the antioxidant capacity of chickpeas by solid state fermentation with Cordyceps militaris SN-18. J. Funct. Foods 10:210–222 (2014)
Terpinc P, Čeh B, Ulrih NP, Abramovič H. Studies of the correlation between antioxidant properties and the total phenolic content of different oil cake extracts. Ind. Crops Prod. 39:210–217 (2012)
Su MS, Chien PJ. Antioxidant activity, anthocyanins, and phenolics of rabbiteye blueberry (Vaccinium ashei) fluid products as affected by fermentation. Food Chem. 104:182–187 (2007)
Jin HJ, Lee JH, Kim DH, Kim KT, Lee GW, Choi SJ, Chang PS, Paik HD. Antioxidative and nitric oxide scavenging activity of branched-chain amino acids. Food Sci. Biotechnol. 24:1555–1558 (2015)
Jung CH, Seog HM, Choi IW, Park MW, Cho HY. Antioxidant properties of various solvent extracts from wild ginseng leaves. LWT-Food Sci. Technol. 39:266–274 (2006)
Mraihi F, Journi M, Chérif JK, Sokmen M, Sokmen A, Trabelsi-Ayadi M. Phenolic contents and antioxidant potential of crataegus fruits grown in Tunisia as determined by DPPH, FRAP, and β-carotene/linoleic acid assay. J. Chem. 2013:378364 (2013)
Lu ZH, Peng HH, Cao W, Tatsumi E, Li LT. Isolation, characterization and identification of lactic acid bacteria and yeasts from sour Mifen, a traditional fermented rice noodle from China. J. Appl. Microbiol. 105:893–903 (2008)
Kim DH. Chemical diversity of Panax ginseng, Panax quinquifolium, and Panax notoginseng. J. Ginseng Res. 1:1–15 (2012)
Theriault M, Caillet S, Kermasha S, Lacroix M. Antioxidant, antiradical and antimutagenic activities of phenolic compounds present in maple products. Food Chem. 98:490–501 (2006)
Waszkiewicz N, Szajda SD, Duchnowska EK, Sawko A, Nammous H, Buko V, Szulc A, Zwierz K, Ladny JR. Serum β-glucuronidase as a potential colon cancer marker: a preliminary study. Postepy Hig. Med. Dosw. 69:436–439 (2015)
Chen CYO, Ribaya-Mercado JD, McKay DL, Croom E, Blumberg JB. Differential antioxidant and quinone reductase inducing activity of American, Asian, and Siberian ginseng. Food Chem. 119:445–451 (2010)
Jung HW, Kim JE, Seo JH, Lee SP. Physicochemical and antioxidant properties of red ginseng marc fermented by bacillus subtilis ha with mugwort powder addition. J. Korean Soc. Food Sci. Nutr. 39:1391–1398 (2010)
Lee SE, Lee SW, Bang JK, Yu YJ, Seong NS. Antioxidant activity of leaf, stem, and root of Panax ginseng C. A. Meyer. Korean J. Medicinal Crop Sci. 12:237–242 (2004)
Park SN, Choi SW, Boo YC, Kim CK, Lee TY. Effect of flavonoids of ginseng leaves on erythrocyte membranes against singlet oxygen caused damage. Korean Soc. Ginseng. 7:49–57 (1990)
Kong YH, Lee YC, Choi SY (2009) Neuroprotective and anti-inflammatory effects of phenolic compounds in Panax ginseng C.A. Meyer. J. Ginseng Res. 33:111–114
Takashima M, Kanamori Y, Kodera Y, Morihara N, Tamura K. Aged garlic extract exerts endothelium-dependent vasorelaxant effect on rat aorta by increasing nitric oxide production. Phytomedicine 24:56–61 (2017)
Wall C, Lim R, Poljak M, Lappas M. Dietary flavonoids as therapeutics for preterm birth: Luteolin and kaempferol suppress inflammation in human gestational tissues in vitro. Oxid. Med. Cell. Longev. 2013:485201 (2013)
Acknowledgements
This research was supported by the Priority Research Centers Program through the National Research Foundation funded by the Ministry of Education, Science, and Technology (Korea) (2009-0093824); by the Ministry of Agriculture, Food, and Rural Affairs (Korea, 314020-2).
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Eom, S.J., Hwang, J.E., Kim, KT. et al. Increased antioxidative and nitric oxide scavenging activity of ginseng marc fermented by Pediococcus acidilactici KCCM11614P. Food Sci Biotechnol 27, 185–191 (2018). https://doi.org/10.1007/s10068-017-0207-y
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DOI: https://doi.org/10.1007/s10068-017-0207-y