Abstract
Cytomegaloviruses (CMVs) have developed multiple diverse strategies to ensure their replicative success and to evade immune recognition. Given the fact that G protein-coupled receptors (GPCRs) are key regulators of numerous cellular processes and modify a variety of signaling pathways, it is not surprising that CMVs and other herpesviruses have hijacked mammalian GPCRs during their coevolution. Human cytomegalovirus (HCMV) encodes for four viral GPCR homologues (vGPCRs), termed US27, US28, UL33, and UL78. Although HCMV-encoded GPCRs were first described in 1990, the pivotal functions of these viral receptor proteins were detected only recently. Here, we summarize seminal knowledge on the functions of herpesviral vGPCRs with a focus on novel roles of cytomegalovirus-encoded vGPCRs for viral spread and the regulation of latency.
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Introduction
G protein-coupled receptors (GPCRs), also termed seven-transmembrane receptors, constitute the largest and most diverse family of eukaryotic membrane receptors involved in signal transduction. Noteworthy, a tremendous diversity of physiological processes is substantially regulated by GPCRs, such as neuro-transmission, angiogenesis, cell proliferation, and death, as well as activation and suppression of the immune system [1,2,3,4,5]. Hence, it is not surprising that GPCRs have emerged as crucial players in viral entry and spread, modulation of the host immune system, and assurance of cell survival after infection. Moreover, various herpes- and poxviruses encode homologous versions of cellular GPCRs (vGPCRs), which contribute to the dysregulation of cellular signaling processes [6,7,8,9,10,11]. Whereas most of these viruses express one or two vGPCRs, human cytomegalovirus (HCMV) encodes four vGPCRs, termed US27, US28, UL33, and UL78 [7, 12]. Although vGPCRs are phylogenetically related to human seven-transmembrane receptors, they exhibit considerable differences in characteristics including their sorting and signaling capacities. Since vGPCRs emerge as important determinants of viral pathogenicity, there is growing interest to utilize the pharmacologic targeting of vGPCRs as a novel antiviral principle.
Structure and function of cellular G protein-coupled receptors
With more than 800 members, G protein-coupled receptors (GPCRs) constitute the largest and most diverse family of membrane receptors [13]. Due to their role as key regulators of numerous cellular processes, GPCRs exhibit central relevance to the current clinical practice of medicine. As highly valued drug targets, 50–60% of all currently available pharmaceuticals modulate GPCR functions, directly or indirectly. The wide range of therapeutic effects thereby counteracts various symptoms including pain, allergic rhinitis, schizophrenia, or hypertension [14,15,16].
GPCRs are located at the cytoplasmic membrane where they mediate cellular responses to a multitude of signals, such as neurotransmitters, hormones, calcium ions, chemokines, sensory stimuli, or nucleotides [17, 18]. Interestingly, for some GPCRs, the so-called orphan receptors, no ligands have been identified so far, however, they often modulate activities of other receptors through the formation of heteromers [19, 20]. Although it was long believed that GPCRs function as monomers, recent reports have highlighted that GPCR activation is not restricted to ligands, but can also occur via interaction with another receptor [21]. The activation of a GPCR by extracellular stimuli or multimerization induces a conformational change of the receptor, which in turn activates an intracellular signaling cascade [22]. Even though GPCRs regulate an enormous diversity of cellular mechanisms, they share a common structure, which is highly conserved in many eukaryotes. GPCRs, also termed seven-transmembrane-spanning receptors, consist of seven-transmembrane-spanning α-helical segments, which are connected via three intracellular loops (i1, i2, i3) and three extracellular loops (e1, e2, e3) [23]. Disulfide bonds between conserved cysteine residues in the extracellular loops e1 and e2 stabilize the structure of a GPCR [24]. Whereas the C-terminal domain is localized intracellularly to interact with effector molecules, the N-terminal tail, which is usually glycosylated, faces the extracellular environment and possesses an important function in ligand binding [24]. Due to the presence of conserved amino acid residues, the superfamily of GPCRs is subdivided into five main families, named glutamate, rhodopsin, adhesion, frizzled/taste2, and secretin GPCRs [1].
Heterotrimeric G proteins are the common factor to transmit signals of GPCRs across the plasma membrane to activate intracellular signaling pathways [18, 25]. A highly conserved Asp-Arg-Tyr (DRY) motif in the C-terminal region of the third transmembrane domain of GPCRs is thought to be the crucial site of G protein binding [26]. G proteins consist of three subunits termed Gα, Gβ, and Gγ. The Gα subunit binds either guanosine diphosphate (GDP) in its resting state or guanosine triphosphate (GTP) when activated. Upon agonist binding, a conformational change of the 7TM occurs, which is followed by an exchange of GDP to GTP and the dissociation of the Gα-subunit from the Gβ and Gγ subunits, which are closely bound to one another, referred to as Gβγ [27]. The Gβγ and Gα subunits of the G protein stimulate a vast number of effector molecules, whose nature highly depends on the Gα subunit specificity of the respective GPCR [28]. Gα proteins are classified into four subfamilies, termed Gαs, Gαi, Gαq, and Gα12. Stimulation of Gαs is known to activate the adenylyl cyclase (AC) and to increase levels of cyclic adenosine monophosphate (cAMP). In contrast, activated Gαi proteins inhibit the induction of AC. Moreover, Gα12 leads to an activation of the Rho family of GTPases and through Gαq coupling, GPCRs induce phospholipase C (PLC) activity and, consequently, cleavage of phosphatidylinositol biphosphate (PIP2) into diacylglycerol (DAC) and inositol triphosphate (IP3) as well as the NF-κB pathway [29,30,31]. Notably, although the exact mechanism of NF-κB activation by GPCRs remains largely elusive, recent reports have shown that the signaling cascade involves the scaffold protein CARMA3 together with the BCL10/MALT1 complex [32, 33]. Besides, signaling can result from activated Gβγ, which induces the activity of phospholipases, ion channels, or lipid kinases [27]. Interestingly, in addition to this “classical” transduction pathway, GPCRs have been demonstrated to activate G protein-independent signals via further scaffold proteins including arrestins [34].
Herpesviral vGPCRs
The appearance of viral G protein-coupled receptors (vGPCRs) in genomes of herpesviruses is probably a result of viral hijacking during coevolution with the respective hosts. While α-herpesviruses do not encode GPCR homologues, the β-herpesviral genomes contain several vGPCRs and the γ-herpesviral genomes contain at least one vGPCR (Fig. 1). The respective proteins resemble human chemokine receptors in structure and function and are highly suspected and reported to promote immune evasion and viral dissemination [35]. Chemokine receptors are classified according to the specific subclass of chemokines that they respond to in CXC receptors (CXCR1-6), CC receptors (CCR1-10) as well as the CX3CR1 and XCR1 receptors. Chemokines are chemotactic cytokines and comprise the largest family of cytokines, consisting of 43 so far identified endogenous chemokine ligands in humans. They are divided into four families: C, CC, CXC and CX3C chemokines, which is based on the number and arrangement of conserved cysteine residues in the N-terminus of chemokines that form disulfide bonds for stabilization of the biologically important tertiary structure. The γ-herpesvirus Kaposi’s sarcoma-associated herpesvirus (KSHV) infects mainly endothelial cells but also B cells, monocytes, macrophages and DCs [36, 37]. Latent infection is mainly asymptomatic, but may manifest as Kaposi’s sarcoma (KS) lesions, which are highly vascularized neoplasms often visible as red patches on the skin. KSHV encodes a single vGPCR, named ORF74, which shows highest sequence homology to human CXCR2. Interestingly, ORF74 binds a broad range of human chemokines including CXCL1-8, CXCL10, CXCL12, CCL1 and CCL5 [9] (Fig. 1). ORF74 is expressed in KS lesions and was identified as an important factor for the initiation and development of KS as suggested by experiments in transgenic mice [38]. Also, the genome of Epstein–Barr virus (EBV) encodes for one GPCR homologue, referred to as BILF1, which is transcribed as an early gene. EBV infects mainly B cells and epithelial cells and was one of the first discovered human tumor viruses. BILF1 shares highest sequence homology with CXCR4 and constitutively couples to Gαi/o proteins but no ligands have been identified to target BILF1 hitherto (Fig. 1). Several reports suggest that BILF1 was evolved by EBV as versatile immunomodulatory protein to promote viral persistence. BILF1 reduces the levels of MHC class I at the cell surface to inhibit CD8(+) T-cell recognition of infected cells [39]. Furthermore, BILF1 inhibits the phosphorylation of RNA-dependent protein kinase R (PKR), thus antagonizing this important innate defense mechanism [40]. The roseoloviruses HHV-6A, HHV-6B and HHV-7 also encode vGPCRs: the early gene products U12 and U51, which are expressed late during infection [41, 42]. HHV-6A/B-encoded U12 shares highest homology with CCR10, while HHV-7-encoded U12 is most similar to CX3CR1. U51 of HHV-6 shares the highest homology with CCR7, while U51 from HHV-7 is closest to CCR2 (Fig. 1). However, their functions during lytic infection or latency are largely unknown [35].
Functions of cytomegalovirus-encoded GPCRs
HCMV encodes four GPCR homologues, termed US27, US28, UL33, and UL78 [7, 12, 43] (Fig. 1). While UL33 and UL78 exhibit a high conservation among all cytomegaloviruses, US27 and US28 are restricted to primate CMVs. Many reports have demonstrated that expression of these vGPCRs is not essential for viral replication in vitro [44, 45]. Furthermore, due to the strict specificity of CMVs for their respective hosts, the in vivo characterization of the role of HCMV-encoded vGPCRs is hampered. Thus, initial studies concentrated on the definition of ligand binding and signaling activities of the HCMV vGPCRs using cell culture models.
US28 is the most intensely studied HCMV-encoded vGPCR and seems to be a versatile viral tool (recently reviewed in [46]). US28 is the only HCMV-encoded vGPCR that responds to different human chemokines like CCL2 (MCP1; monocyte chemoattractant protein 1), CCL3 (MIP-1α; macrophage inflammatory protein 1-alpha), CCL4 (MIP-1β; macrophage inflammatory protein 1-beta), CCL5 (RANTES; regulated on activation, normal T cell expressed and secreted) or CX3CL1 (fractalkine) and induces various signaling pathways in response to promiscuous G protein-dependent signaling (Figs. 1, 2) [47,48,49,50]. Responses to its chemokine ligands include induction of a calcium flux due to PLCβ or ERK2 activation and induction of SMC migration via SCR- or RhoA-dependent signaling [48, 51,52,53]. However, US28 was also described to signal constitutively in a ligand-independent manner which activates PLCβ and NF-κB, STAT3, NFAT and CREB via interaction with Gαq or Gαi as well as Gβ/Gγ subunits [54,55,56,57]. Parts of these signaling events seem to promote proliferative signals during lytic HCMV infection thereby associating US28 with vascular diseases and potential oncomodulatory effects [8, 51, 52]. US28 undergoes fast and constant endocytosis and recycling and is therefore mostly found in intracellular vesicles in vitro [58, 59]. In this context, US28 was also identified to act as a chemokine sink by binding and internalizing chemokines thereby withdrawing them from the environment of infected cells [45, 53, 60, 61]. Meanwhile, structural details for chemokine recognition are available, since the crystal structure of US28 in complex with the chemokine domain of human CX3CL1 could recently be solved [62]. This is an important step forward since it allows for the structure-based discovery of US28 small molecule ligands that could be used for pharmacological modulation of this vGPCR [63].
So far, no ligands for the vGPCRs US27, UL78, and UL33 could be identified. Thus, these proteins are still orphan receptors (Figs. 1, 2). UL33 and its homologues in mouse (M33) and rat CMV (R33) are able to activate several signaling pathways in a constitutive manner [67,68,69,70]. For the rodent homologues M33 and R33, a critical role for infection of the host could be demonstrated [71,72,73]. Gene-knockout viruses were used to show that deletion of M33 or R33 resulted in less virulent CMV variants which no longer replicated in salivary glands. Intriguingly, the in vivo replication defect of an M33-deficient virus was partially complemented by UL33 and US28. This strongly suggests that the respective vGPCRs share biological functions [74].
In contrast to US28 and UL33, the signaling activities of US27 and UL78 are not well characterized. US27 and US28 lie directly adjacent to each other in the viral genome and share 31% sequence identity. The results of a recent phylogenetic analysis indicate that the human chemokine receptor CX3CR1 served as the common ancestor and subsequent gene duplication gave rise to two vGPCRs which evolved distinct functions during virus infection [75]. Since US27 is heavily glycosylated and possesses two conserved cysteine residues in the second and third extracellular loop, a potential involvement of US27 in chemokine binding appears possible, however, no ligands could be identified so far [76]. Expression of US27 in multiple cell types is described to result in two remarkable phenotypes. First, US27 expression enhances cell proliferation and survival, which was linked to suppression of negative growth regulators [77]. Second, in contrast to UL33 and UL78 that are described to reduce CXCR4 receptor functions, US27 increases CXCR4 expression levels and CXCL12-promoted signaling of CXCR4 [78, 79]. Alterations of CXCR4 internalization dynamics in the presence of US27 resulting in prolonged intracellularly located CXCR4 with delayed recycling kinetics were suggested as the mechanism for this observation [80]. Regarding its subcellular localization, US27 is mainly associated with the endosomal machinery and undergoes constitutive endocytosis in transient and stable transfection systems [81]. Interestingly, the C-terminal domain of US27 was suggested to confer the primarily intracellular localization of US27 [82]. However, the function of US27 during infection is poorly understood. The US27 gene is highly conserved among HCMV strains including clinical isolates and laboratory strains indicating an important role during infection [75, 83]. Viral mutants lacking the US27 gene are replication competent but are limited to spreading from cell-to-cell rather than by the extracellular route [84]. The localization of US27 in the viral envelope is consistent with this finding and suggests a role during entry or egress [81]. Upon de novo synthesis, US27 is expressed with late expression kinetics. Starting at 48 hpi, US27 is mainly found in the perinuclear structures associated with the cVAC [81, 85]. A recent approach to identify novel signaling capabilities of the HCMV-encoded vGPCRs with special interest in the poorly characterized US27 and UL78, revealed a strong and so far undescribed NF-κB activation by US27 which is assumed to be relevant for viral dissemination [86].
Knowledge about the role of UL78 during viral infection is limited. Studies using UL78-deficient viral strains indicated a role of this vGPCR for viral entry and efficient replication in epithelial cells but not in fibroblasts [87]. Furthermore, the rodent homologues M78, encoded by murine CMV and R78, encoded by rat CMV, were utilized to investigate the relevance of this vGPCR for viral pathogenesis in vivo [88,89,90]. Deletion of R78 from rat cytomegalovirus (RCMV) strains resulted in considerably higher survival compared to wild-type (wt)-infected animals [88]. In addition, studies using the murine cytomegalovirus (MCMV) homologue M78 revealed that UL78 might be involved in tissue tropism, since deletion of M78 did not affect dissemination in general but attenuated the replication of MCMV in the salivary glands of infected mice [89]. Similar to the three other vGPCRs of HCMV, UL78 undergoes constitutive internalization [91]. Wagner et al. reported that UL78 can be detected at the plasma membrane but it mainly localizes to the endoplasmic reticulum. Furthermore, using several in vitro assays, they were able to demonstrate that UL78 forms heteromers with US28. This appears to be important for a silencing of US28-mediated activation of NF-κB-dependent gene expression by UL78 [92]. Besides, UL78, together with UL33, has been shown to disturb the cell surface expression, ligand-induced internalization, and signal transduction of the cellular chemokine receptors CCR5 and CXCR4, which was caused by receptor heteromerizations [79]. Consequently, UL78, similar to US27, seems to be more than just an orphan receptor and requires further investigation to unravel its main purpose for efficient HCMV replication.
Role of CMV-encoded vGPCRs for viral spread via the regulation of DC migration
One recently emerging role of vGPCRs with high importance for pathogenesis pertains to the regulation of dendritic cell migration during viral dissemination [93]. Already in 2008, it was detected that mutations of the murine CMV vGPCR M33 which abrogate constitutive signaling result in significantly diminished MCMV infection of the salivary glands [73]. UL33 of HCMV was shown to be able to functionally substitute for M33 in vivo suggesting conserved biological roles of these vGPCRs. A further characterization of the phenotype of M33 knockout viruses revealed an additional attenuation for infection of the spleen and pancreas as well as a severe defect in reactivation from latency indicating tissue-specific functions of M33 during infection [71]. Although infection experiments conducted in immunocompromised mice suggested that M33 only plays a role for viral amplification once the virus reaches the salivary gland, recent studies indicate a more profound role of M33 dictating efficient viral dissemination [94, 95]. Farrell and colleagues reported that following intranasal infection, MCMV migrates via lung CD11c+ dendritic cells (DC) to lymph nodes (LN), blood and then salivary glands. This route requires that infected dendritic cells do not only enter LNs but they also need to recirculate into blood to mediate dissemination. However, although traffic to draining LNs is well recognized, DCs usually do not leave LNs but are thought to die locally following arrival in LNs [96]. This suggested that MCMV has evolved specific mechanisms to stimulate the exit of infected DCs from LNs thus fostering viral dissemination. It could be shown that MCMV-infected DCs exited LNs not via efferent lymph but via specialized vessels, the so-called high endothelial venules (HEV) which represent major sites of extravasation of lymphocytes from the blood into the LN via a multistep adhesion process (Fig. 2) [97]. As cell migration is controlled by chemokines, viral chemokine receptor homologues were suspected to potentially mediate the LN traverse of infected DCs. Indeed, infection experiments with MCMV harboring a knockout of the chemokine receptor M33 resulted in an accumulation of infected DCs in LNs.
Consequently, viral spread was greatly reduced [97]. Since an M33 point mutant lacking Gq signaling exhibited an identical phenotype, the constitutive signaling activity of M33 appears to be required to promote the exit of infected DCs from LNs via HEVs (Fig. 3). Furthermore, US28 could substitute for M33 in promoting DC recircularization while signaling-deficient US28 was inactive [97]. This suggests that a similar mechanism of vGPCR-driven viral dissemination via DC recircularization which may function through the downregulation of DC retention signals could also be true for HCMV infection. In summary, these experiments indicate a key role for cytomegalovirus vGPCRs in systemic viral spread.
Regulation of HCMV latency by the vGPCR US28
Several studies reported that the HCMV-encoded vGPCR US28 is expressed during lytic infection and latency [98, 99]. However, its function during latency has remained undefined for a long time and is only recently beginning to be elucidated. Humby and O’Connor were the first to demonstrate that US28 was required during latency either in an in vitro latency model or using primary ex vivo-cultured CD34(+) hematopoietic progenitor cells (HPCs) [100]. They observed that infection of CD34(+) HPCs with viruses lacking the US28 gene resulted in transcription from the major immediate early promoter and the production of infectious virus. Mechanistically, it is suggested that US28 attenuates mitogen-activated protein kinase (MAPK) and NF-κB signaling as well as c-fos expression in latently infected cells, which is required for suppression of the HCMV major immediate early promoter (MIEP) to prevent lytic infection [101, 102]. This is unexpected since previous studies reported that a similar set of signal transduction pathways is utilized by US28 to activate the MIEP upon infection of permissive cell types [103]. This strongly stresses the cell-context dependency of vGPCR signaling. An alternative mechanism was proposed by Zhu et al.: they described a requirement for US28 to activate the STAT3-iNOS-NO axis which reprograms HPCs into a unique monocyte subset to achieve latency [104]. Thus, although different studies concordantly demonstrate a requirement for US28 signaling to maintain HCMV latency in CD34(+) progenitor cells and CD14(+) monocytes, the exact way how US28 generates a host environment conducive to latency requires further investigation.
Importantly, however, those studies strongly support the notion that US28 might serve as an appealing drug target during latency. On the one hand, inverse agonists may be used to interfere with US28 signaling. This induces viral reactivation in latently infected cells [101]. Consequently, cytotoxic T-cell killing of these normally immunologically undetectable cells may help to purge the latent viral reservoir [105]. Although the potency of the presently available inverse agonists of US28 (e.g. VUF2274) is limited, there is an ongoing intense search for optimized drugs for pharmacological modulation of this vGPCR [106]. An alternative strategy makes use of the fact that US28 is a membrane protein exhibiting a high rate of constitutive internalization thus efficiently sequestering chemokines from the environment [45]. This inspired the concept of using chemokine-based immunotoxins to target cytomegalovirus-infected cells. Spiess et al. designed a synthetic CX3CL1 variant with increased affinity and specificity for US28 that was fused with the cytotoxic domain of Pseudomonas Exotoxin A [107]. This immunotoxin not only caused a direct killing of lytically infected cells but the specific targeting of latently infected cells could also robustly reduce virus reactivation [107, 108]. Although concerns regarding potential side effects of such an immunotoxin-based antiviral approach may delay its use in vivo, the ex vivo depletion of latently infected HPCs before stem cell transplantation may constitute an alternative feasible approach to decrease the burden of latent HCMV.
Concluding remarks
Although cytomegalovirus-encoded vGPCRs have first been detected approximately 30 years ago, only recently novel functions of these signal transduction molecules with utmost importance for viral pathogenesis could be unraveled. On the one hand, there is accumulating evidence that vGPCR signaling is required for systemic viral spread via DC recircularization. On the other hand, interference with vGPCR signaling interrupts latency fostering viral reactivation. Thus, cytomegalovirus-encoded vGPCRs emerge as an Achilles heel of cytomegaloviruses, since pharmacological modulation of these receptors in combination with conventional drugs like ganciclovir may not only arrest active infection but could also help to reduce the latent viral reservoir.
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Acknowledgements
This work was supported by the Deutsche Forschungsgemeinschaft (STA357/7-1), the Wilhelm-Sander-Stiftung (2016.087.1) and the Kompetenznetzwerk Zytomegalie Baden-Württemberg (KSKV002).
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Frank, T., Niemann, I., Reichel, A. et al. Emerging roles of cytomegalovirus-encoded G protein-coupled receptors during lytic and latent infection. Med Microbiol Immunol 208, 447–456 (2019). https://doi.org/10.1007/s00430-019-00595-9
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DOI: https://doi.org/10.1007/s00430-019-00595-9