Abstract
Freshwater lakes in the Hudson Bay Lowlands (HBL) area of Ontario are expected to undergo considerable physical, chemical and biological changes related to climatic change; however, the nature of those changes is still very uncertain. As a first step to improve our understanding of fish communities within these subarctic lakes, we aimed to: (a) characterize trophic dynamics of several large-bodied species within three HBL lakes and (b) determine whether trophic dynamics of selected species in the HBL lakes differed from the same species in Southern Ontario lakes. We found that species-specific trophic position and littoral resource reliance varied significantly within and among the HBL lakes of differing biological communities, chemistry and morphometry. Although several significant differences were evident among lakes in the northern and southern regions, we did not find striking consistent differences in trophic dynamics. Based on observations of high variation in trophic position and/or littoral reliance, we can hypothesize that changes in food resources resulting from climatic change would have little impact on most of the large-bodied species.
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Introduction
Climatic change is emerging as a driving force behind many ecological changes within freshwater ecosystems (Mooij et al. 2005; Moss 2010). Some climatic factors that can influence biological communities in aquatic environments include changes in temperature, precipitation, water residence time, water level and ice cover (Jensen et al. 2007; Whitehead et al. 2009; Carter and Schindler 2012). Alteration of these factors can directly impact individual species or lead to shifts in community composition, biodiversity and altered trophic interactions among species (Brown et al. 2007; Keller 2007; Chessman 2009; Kratina et al. 2012).
The climate of Northern Ontario is expected to change dramatically by the latter part of this century. The northernmost areas are predicted to experience a substantial increase in annual (5–7 °C) and winter temperatures (9–10 °C) (Colombo et al. 2007). Consequently, freshwater lakes in the Hudson Bay Lowlands (HBL) area of Ontario are expected to undergo considerable physical, chemical and biological changes related to climatic change (McKenney et al. 2010; Rühland et al. 2013); however, the nature of those changes is still very uncertain, especially for biota in lakes at higher trophic levels such as fish.
The HBL comprise one of the largest wetland complexes in the world and contain globally significant carbon stores (Macrae et al. 2004). The area is high in biodiversity and very sensitive to climate change (Far North Science Advisory Panel 2010). There are thousands of lakes and ponds across the landscape. Lakes of the HBL are largely unaffected by anthropogenic stressors such as acid rain and nutrient enrichment; however, given its ecological importance study of this area is key to developing an understanding of the potential impacts of climate change. Even though the HBL lakes are in a climatically sensitive region, they have received little research attention (Abraham et al. 2011); thus, there is generally a lack of information regarding how a warmer climate is likely to alter the biological communities within them.
In light of recent evidence of significant changes in lower trophic levels (plankton) from paleolimnological records within HBL lakes (Brazeau et al. 2013; Rühland et al. 2013), it is important that we assess whether and how fish communities will respond to climatic change. Climate change has the potential to directly and indirectly impact fish communities because they are ectotherms, and their activities and trophic interactions are highly dependent on temperature. Furthermore, as integrators in lake food webs (Vander Zanden and Vadeboncoeur 2002), it is of vital importance that we understand whether and how fish communities will change because loss or significant alterations in fish abundance or size composition can create changes that cascade throughout aquatic ecosystems.
One of the major challenges in freshwater ecology is predicting the consequences of environmental change on ecosystem structure and function. As a first step to improve our understanding of whether and how climate change can potentially alter food web structure and function of HBL lakes, we compare fish populations from the HBL lakes to those in warmer areas of Ontario including the Muskoka-Haliburton and Kawartha-Renfrew regions. Specifically, our objective is to determine whether trophic dynamics differ substantially among populations of several large-bodied fish species (that is, dominant piscivores and benthivores) in Northern versus Southern Ontario regions. This study is the first to apply stable isotope analyses to the examination of food web structure in subarctic HBL lakes. Stable isotope analysis is a very useful molecular technique for tracing energy flows and examining trophic relationships among predators and their prey and has been widely applied in aquatic food web studies (Peterson and Fry 1987; Fry 2006). We used carbon and nitrogen stable isotope data for two purposes here: (a) to characterize trophic interactions, energy transfer routes and niche overlap among large-bodied fish within three HBL lakes that are located in the Sutton Ridges area, a unique geological area within the HBL that offered the widest range of lake types, and (b) to determine whether the dietary carbon source and trophic position for large-bodied fish species differ between the HBL lakes in the north and southern Muskoka-Haliburton and Kawartha-Renfrew lakes. We hypothesize that the wide ranging chemical, biological and morphometric differences among HBL lakes in the study area will result in significant variability of trophic interaction and energy transfer routes of selected large-bodied species within and among lakes. Further, if climate plays an overriding influence on trophic dynamics and energy transfer even in such variable systems, we would then expect that there will be significant and consistent differences between the northern HBL populations and those found in the southern lakes.
Methodology
Study lakes
Our data set consists of a total of nine lakes, three from each region (Table 1; Fig. 1). We used available data to select lakes in the Muskoka-Haliburton and Kawartha-Renfrew areas that contained at least one of the species found in the HBL lakes to facilitate comparison between regions. Within a given region, we selected lakes that ranged widely in morphometry and chemical characteristics. Lake areas and maximum depths ranged from 1,230 to 2,720 ha and 1.7–35.2 m in the HBL, 59–1,240 ha and 10–64 m in the Kawartha-Renfrew region, and 43.9–93.2 ha and 12–38 m in the Muskoka-Haliburton region.
The geology of the surrounding lake catchments differs among regions. Catchments of the Kawartha-Renfrew lakes consist predominantly of calcareous bedrock, whereas the bedrock of the Muskoka-Haliburton lakes is primarily the granitic gneiss characteristic of the Precambrian Shield. The HBL lake catchments are more complex with a combination of Precambrian bedrock influencing the higher elevation lakes of the Sutton Ridge and more recent Paleozoic or calcareous bedrock surrounding the lower elevation lakes.
The difference in geology among and within regions is reflected in the chemistry of the lakes; consequently, alkalinity, dissolved organic carbon (DOC) and total phosphorus (TP) concentrations are comparatively higher in the Kawartha-Renfrew and HBL lakes than in the Muskoka-Haliburton lakes (Table 1). Based on the Organization of Economic Cooperation and Development (OECD) classification (OECD 1982), the Muskoka-Haliburton lakes were all oligotrophic (TP range = 3.1–5.8 μg L−1), whereas the HBL lakes ranged from oligotrophic to mesotrophic (6.6–19.7 μg L−1), and the Kawartha-Renfrew lakes from mesotrophic to eutrophic (15.1–43.6 μg L−1). Ranges in alkalinity in the HBL, Kawartha-Renfrew and Muskoka-Haliburton lakes were 63.1–106.0 mg L−1 CaCO3, 75.0–123.0 mg L−1 CaCO3 and 3.9–4.7 mg L−1 CaCO3, respectively; whereas ranges in DOC concentrations were 5.8–9.8, 5.5–10.0 and 4.0–6.6 mg L−1, respectively. The pH ranged from 7.6–8.1, 8.0–8.1 and 5.8–6.1 in the HBL, Kawartha-Renfrew and Muskoka-Haliburton lakes, respectively.
Climatically, the HBL is considerably cooler and drier than the Kawartha-Renfrew and Muskoka-Haliburton regions. The mean annual temperature for the HBL region between 2000 and 2010 was −5.7 ± 1.5 °C (mean and standard deviation for Churchill, Manitoba station) compared to 7.2 ± 1.1 and 5.4 ± 0.8 °C for the Kawartha-Renfrew (Peterborough and Kingston, Ontario stations) and Muskoka-Haliburton (Haliburton, Ontario station) regions, respectively (Environment Canada, http://www.cccma.ec.gc.ca/hccd/). The total precipitation was lower in the HBL with 558 ± 109 mm compared to 995 ± 118 and 1,184 ± 122 mm for the Kawartha-Renfrew and Muskoka-Haliburton regions, respectively (Environment Canada, http://www.cccma.ec.gc.ca/hccd/).
Biologically, the three HBL lakes differed widely in their phytoplankton, zooplankton, benthic invertebrate and fish communities (Keller et al. 2011, 2013). Phytoplankton biovolume and genera richness were highest in Opinnagau East Lake (1,249 mm3 m−3 and 69, respectively) and lowest in Hawley Lake (271.7 mm3 m−3 and 44, respectively). Zooplankton density was highest in Spruce Lake (5.40 × 104 m−3) and lowest in Hawley Lake (1.91 × 104 m−3), but zooplankton species richness was highest in Opinnagau East Lake (12) and lowest in Hawley Lake (8). The profundal benthic macro-invertebrate community of Hawley Lake (10 species) was richer compared to Opinnagau East Lake (6 species) and Spruce Lake (3 species). The fish species richness and diversity of Hawley Lake (15 species and Shannon H′ = 2.01) were higher than that of Spruce Lake (10 species and Shannon H′ = 1.81). A complete fish survey was not available for Opinnagau East Lake.
Sample collection
Fish and invertebrates
All fish and invertebrate samples used for stable isotope analyses were collected between 2009 and 2010 during the summer months when surface water temperatures were above 18 °C (18–20 °C for HBL, 21–23 °C for Kawartha-Renfrew and 20–21 °C for Muskoka-Haliburton regions). Fish were caught from throughout the lakes using standard gill netting methods developed by the Ministry of Natural Resources for the Broadscale Monitoring Program (Sandstrom et al. 2008) or the NORDIC Index Netting Program (Morgan and Snucins 2005). Multi-mesh gillnets (benthic) consisting of 12 different mesh sizes (stretched mesh sizes of 13–127 mm for Broadscale Monitoring and 10–110 mm for NORDIC Index nets) were randomly distributed across lakes, with the number of net sets dependent on lake area and depth. The number of benthic multi-mesh gillnets set ranged from four to ten in the HBL, 13–33 in the Kawartha-Renfrew region and 24–32 in the Muskoka-Haliburton region. We used either all of the fish caught or a random selection covering the full size range of the total catch obtained from each lake (see Table 2) for stable isotope analyses. For each fish, we recorded length and removed a skinless, dorsal muscle sample from the mid-region above the lateral line for isotope analyses.
Benthic invertebrates were collected by the kick-and-sweep method (David et al. 1998) or an Ekman dredge (Luek et al. 2013). For the Kawartha-Renfrew and HBL lakes, kick-and-sweep sampling was done with a 500-μm mesh net at 5–10 locations within each lake. For the Muskoka-Haliburton lakes, a 15 cm × 15 cm Ekman dredge was used to collect nine samples from three locations within each lake (Luek et al. 2013). All collected samples were sorted to obtain samples of Amphipoda, Chironomidae, Emphemeroptera and Corixidae.
Pelagic zooplankton samples were collected with a series of conical mesh nets. Zooplankton for the Kawartha-Renfrew and HBL lakes were collected with 75- and 200-µm mesh nets, whereas 84- and 128-µm mesh nets were used in the Muskoka-Haliburton lakes to collect zooplankton and Chaoborus, respectively (Luek et al. 2013).
Water chemistry
Water samples for chemical analyses were obtained from 0.5 m below the surface at the deepest station in each lake at the time of fish and invertebrate sampling. Chemical analyses were performed following the standard analytical protocols of the Ontario Ministry of the Environment (Ontario Ministry of the Environment 1983; www.desc.ca).
Stable isotope analyses
Carbon and nitrogen stable isotope analyses of dried and ground fish, zooplankton and benthic invertebrates were conducted in either the Water Quality Center at Trent University, the Environmental Quality Analysis Laboratory at the University of Regina or the Stable Isotope in Nature Laboratory at the University of New Brunswick. These are all accredited laboratories that routinely use a number of certified international standards for calibration when performing isotopic analyses.
For this multi-lake study, we calculated trophic position (TPos) of individual fish using a one source model as follows:
where δ15Nfish is the nitrogen isotope value for fish, δ15Nbase is the isotope value for baseline organisms, 3.4 ‰ is the trophic fractionation value of δ15N and 2 is the trophic position of the primary consumers used as baseline organisms (Post 2002; Anderson and Cabana 2007). Here, we calculated mean δ15Nbaseline values for each study lake using the δ15N of a number of benthic invertebrates (Amphipoda, Chironomidae, Emphemeroptera and Corixidae) and zooplankton obtained from each lake.
Using the carbon isotopic data, we also estimated the reliance on littoral resources as follows:
where δ13Cfish, δ13Cpelagic base and δ13Clittoral base are the fish and baseline carbon isotope values, respectively (adapted from Vander Zanden and Vadeboncoeur 2002). Here, we used zooplankton as the pelagic baseline and a number of benthic invertebrates (Amphipoda, Chironomidae, Emphemeroptera and Corixidae) as the littoral baseline organisms.
Isotopic niche space analysis
We used SIBER (Jackson et al. 2011) in the SIAR package (Parnell and Jackson 2013; R Core Team 2013) to calculate trophic niche space in isotopic dimensions of a given fish species in each lake. This method was first described by Layman et al. (2007) and written into a Bayesian model accessible in the R environment by Jackson et al. (2011). Species-specific trophic niche space was calculated for fish species within each of the study lakes. Then, trophic niche space was compared between fish species within each lake as well as between lakes for each fish species by calculating the likelihood that a given probability of a niche space size of one fish species in a specific lake is (a) larger than the niche of the same fish species in any other lake or (b) larger than the niche of any other fish species in the same lake. Any likelihood 90 % > p < 95 % was defined as marginally significant, while likelihoods >95 % were considered significant.
The SIAR program also allows calculation of the trophic niche overlap between two given species (Jackson et al. 2011). This procedure is however not based on a Bayesian approach but rather uses a standard ellipse area calculation corrected for small sample size (Jackson et al. 2011). The procedure returns an area for each of the two input species trophic ellipses in the isotope space (radius of the standard ellipse represents 1 SD of variance in bivariate space) as well as the area of overlap. For this purpose, calculations were made using the percent of overlap between species focussing on the overlap of the trophic niche space of the top predators in the HBL lakes, namely northern pike (Esox lucius) and lake trout (Salvelinus namaycush) in Hawley Lake, and northern pike and walleye (Sander vitreus) in Spruce Lake.
Statistical analyses
Species-specific comparisons of trophic position and littoral reliance among lakes were carried out with parametric and nonparametric statistics in the JMP 10 statistical program. One-way ANOVAs followed by post hoc Tukey HSD tests were primarily used to test for differences among lakes for a given species. In a few cases where Levene’s test indicated that the assumption of homogeneity of variances was violated, we used nonparametric Wilcoxon tests followed by Dunn’s paired comparisons to determine significant differences among lakes. Bonferroni-corrected critical p values were used to determine significance of ANOVAs and Wilcoxon results when necessary.
Results
Fish in HBL lakes
Isotopic values, trophic dynamics and energy pathways
The carbon and nitrogen isotopic values differed among large-bodied fish species within each of the three HBL lakes (Fig. 2, δ13C: ANOVA and Wilcoxon: p ≤ 0.008; δ15N: ANOVA and Wilcoxon: p ≤ 0.0001). Isotopic variability among species within lakes was highest in Hawley Lake, lowest in Opinnagau East Lake and intermediate in Spruce Lake (Fig. 2).
The calculated trophic position varied among species within the HBL lakes. In general, lake trout, northern pike and walleye occupied the highest trophic positions, whereas lake whitefish (Coregonus clupeaformis) and white sucker (Catostomus commersonii) occupied the intermediate and lowest trophic positions, respectively (Fig. 3). In Hawley Lake, lake trout trophic position (mean trophic position ± SD = 4.75 ± 0.11) was significantly higher (Dunn: p ≤ 0.005) than lake whitefish (3.45 ± 0.35) and white sucker (2.89 ± 0.13), but not northern pike (3.58 ± 0.07). In Spruce Lake, walleye trophic position (4.45 ± 0.16) was significantly higher (Dunn: p ≤ 0.009) than lake whitefish (3.62 ± 0.43) and white sucker (3.29 ± 0.31), but not northern pike (4.23 ± 0.16). In Opinnagau East Lake, northern pike occupied a higher trophic position (3.41 ± 0.17) (Tukey HSD: p < 0.001) than lake whitefish (2.89 ± 0.43) and white sucker (2.90 ± 0.12). Lake whitefish trophic position was not significantly different from white sucker in any of the lakes (p ≥ 0.124).
The estimated littoral resource reliance differed significantly among species (Fig. 3) within all of the HBL lakes (Wilcoxon: p ≤ 0.008). In Opinnagau East Lake, northern pike were more reliant on littoral sources (mean littoral reliance proportion ± SD = 0.98 ± 0.03) compared to lake whitefish (0.72 ± 0.24) and white sucker (0.80 ± 0.20) (Dunn: p ≤ 0.013). In Spruce Lake, northern pike (0.45 ± 0.17) were less reliant on littoral sources compared to lake whitefish (0.65 ± 0.13) (Dunn: p = 0.01). In Hawley Lake, lake trout (0.65 ± 0.14) were less reliant on littoral sources compared to northern pike (0.97 ± 0.03) and white sucker (0.93 ± 0.07).
Niche space
The SIBER model indicated that there were differences in trophic niche space among fish species within lakes, as well as for some fish species among lakes (Table 3). In deep Hawley Lake, northern pike and lake trout occupied the smallest niche spaces of the four sampled fish species. White sucker and lake whitefish were lower in the food web, but occupied significantly larger niches. In Opinnagau East Lake, lake whitefish had a significantly larger niche space than white suckers, but there were no other trophic niche differences among species in the fish community in this shallow lake. In Spruce Lake, the smallest of the three HBL lakes, northern pike and walleye occupied similar sizes of trophic niches, while white suckers had the smallest trophic niche space of the community.
For the three fish species that were present in all of the HBL lakes: northern pike, white sucker and lake whitefish, species-specific analysis of differences in trophic niche spaces among lakes revealed differences in trophic niche sizes only for northern pike and white sucker. Northern pike had the largest trophic niche in Spruce Lake, while niche space in Opinnagau East Lake and Hawley Lake were not different from each other. White sucker exhibited the largest trophic niche space in Hawley Lake, with Opinnagau East Lake and Spruce Lake being significantly smaller, but not different from each other.
The calculated isotopic niche spaces indicated that overlap of northern pike with other predators varied in Spruce Lake and Hawley Lake. Northern pike and walleye had overlapping trophic niche spaces in Spruce Lake (27.7 % of walleye niche space was shared with northern pike, where as 10.4 % of northern pike niche space was shared with walleye), but northern pike and lake trout did not overlap in their trophic niches in Hawley Lake (0 % for the two species).
Regional comparison among fish species and lakes
Trophic dynamics
Significant differences in the trophic position occupied by northern pike, walleye and white sucker were found between northern and southern lakes (Fig. 4; Table 4). The trophic position of northern pike varied among four lakes (Fig. 4a; Wilcoxon p < 0.0001), with the highest trophic positions recorded in the northern Spruce Lake population. The northern pike population from southern Muskrat Lake was significantly different from northern Hawley Lake but not Spruce Lake or Opinnagau East Lake. Walleye trophic position varied among the four lakes in which they were found (Fig. 4b; Wilcoxon p < 0.0001), with the northern population from Spruce Lake having the highest trophic position compared to the southern populations. The southern Little Lake walleye population had a significantly lower trophic position compared to northern Spruce Lake and southern Muskrat Lake and Lower Buckhorn Lake. The trophic position of white sucker differed among five lakes (Fig. 4c; Wilcoxon p = 0.006), with the highest positions recorded within the Spruce Lake population. The trophic position of northern Hawley Lake white suckers was significantly lower than those collected from Red Chalk Main in the south and Spruce Lake in the north.
To determine whether fish size could have affected the above trophic position comparisons, we tested whether size of a given species varied among lakes using total length as an indicator of fish size. There was no difference in body size among lakes for northern pike (ANOVA: F 2,35 = 1.28, p = 0.29, respectively). For white sucker, even though a significant difference in size was evident among lakes (ANOVA: F 3,33 = 4.66, p = 0.009), the regression between total length and trophic position was not significant (Regression: r 2 = −0.01, p = 0.59). For walleye, significant differences in size were evident among lakes (Wilcoxon: χ 2 = 12.10, p = 0.007) and a significant relationship was found between total length and trophic position (Regression: r 2 = 0.34, p < 0.0001).
Energy pathways
Several species-specific differences in the reliance on littoral resources were evident among lakes (Fig. 5; Table 4). For northern pike, the population from northern Spruce Lake had a significantly lower reliance on littoral sources compared to southern Muskrat Lake and northern Hawley Lake and Opinnagau Lake (Fig. 5a, Dunn: p ≤ 0.0001). Walleye from southern Muskrat Lake had a significantly greater littoral reliance compared to northern Spruce Lake and southern Lower Buckhorn (Fig. 5b, Dunn: p ≤ 0.009). Walleye from southern Little Lake also had a greater reliance on littoral sources compared to northern Spruce Lake (Dunn: p = 0.0002). The white sucker population from northern Spruce Lake was less reliant on littoral carbon sources compared to southern Muskrat Lake and northern Hawley Lake (Fig. 5c, Dunn: p ≤ 0.003).
Discussion
Trophic dynamics and energy pathways in the HBL lakes
Overall, the energy pathways and trophic positions of dominant large-bodied fish species were very different among the three HBL lakes which varied in chemistry, morphometry and biological communities. In general, Opinnagau East Lake and Hawley Lake fish were more reliant on littoral food, whereas Spruce Lake fish appeared to be more dependent on pelagic resources. These differences were supported by variability in the phytoplankton and zooplankton communities (Keller et al. 2011, 2013). In Spruce Lake, there were more pelagic basal food resources as both phytoplankton biovolume and zooplankton density were high; however, the similarity between zooplankton and benthic invertebrate carbon isotopic values suggests that there may have been some exchange of carbon resources between the littoral and pelagic pathways. In contrast to Spruce Lake, in deeper oligotrophic Hawley Lake, the pelagic pathway was weaker as both phytoplankton biovolume and zooplankton density were the lowest among the three HBL lakes. This is further supported by lake chemistry which shows that Hawley Lake has a lower TP concentration, hence lower pelagic productivity, compared to Spruce Lake (6.6 and 10.6 µg L−1, respectively). In mesotrophic Opinnagau East Lake, fish appeared to be primarily littoral despite a high open water phytoplankton biovolume and moderate zooplankton density because of the shallow nature of the lake.
In Hawley Lake, the differences in trophic position and lack of overlap in isotopic niche area for piscivorous northern pike and lake trout indicate that they were likely exploiting different food sources and habitats. A separation in diet is possible for these piscivores since Hawley Lake is relatively deep; thereby accommodating physical habitat separation with lake trout primarily occupying deeper coldwater and northern pike remaining in fairly shallow but cool water (Scott and Crossman 1973; Casselman and Lewis 1996). Diet separation was also facilitated by the ample abundance of prey species within the lake since 12 species of the resident 15 species (Keller et al. 2011) can readily serve as potential food sources. Lake whitefish was one of two large-bodied planktivores that could have served as an important route of pelagic energy for lake trout; whereas white sucker was one of two large-bodied benthivorous fish species that served as an important energy route of littoral carbon to piscivorous northern pike.
In Hawley Lake, the differences in trophic position between lake whitefish and white sucker suggest that they were utilizing very different habitats and/or food sources. Typically, white suckers are opportunistic benthic feeders capable of foraging on a wide array of benthic resources (Scott and Crossman 1973). In contrast, lake whitefish can be pelagic planktivores even though they can exploit benthic resources when competitors are present (Carl and McGuiness 2006).
In Spruce Lake, the comparable littoral reliance, trophic position and isotopic niche overlap of piscivorous northern pike and walleye indicate that they probably share many food sources in common and/or they were consuming food sources with similar isotopic values. Coexistence between pike and walleye was likely facilitated by spatial and temporal differences in prey selection and feeding habits even though there was some niche overlap between species. While large individuals of walleye and northern pike can venture and forage within both the littoral and pelagic area (Scott and Crossman 1973; Andersen et al. 2008; Bozek et al. 2011), body size differences between species lead to differences in prey selection. Spruce Lake contains a moderately rich prey fish community with eight species of the ten resident fish species (Keller et al. 2011) serving as potential prey. Further, northern pike is primarily a diurnal feeder which contrasts with the crepuscular feeding of walleye (Scott and Crossman 1973; Ryder 1977).
The shallow nature of Opinnagau East Lake appears to influence trophic interaction among the fish species present, resulting in northern pike primarily feeding in the littoral zone and lake whitefish and white sucker having similar trophic dynamics and energy pathways. A strong littoral energy pathway to pike is expected in a shallow waterbody such as Opinnagau East Lake. The nearly identical trophic position and similar littoral reliance of lake whitefish and white sucker in this very shallow lake (Z max = 1.7 m) indicate that they were feeding on a comparable composition of food items. The diet similarity between these two species may be a result of the very shallow nature of Opinnagau East Lake with little opportunity for the typical separation of open water and littoral food sources. The trophic dynamics or isotopic overlap described above for the three species in Opinnagau East Lake is unusual for deeper thermally stratified lakes, but is likely a common scenario across the HBL landscape since the majority of lakes are very shallow.
Potential climate change impacts on fish in the HBL lakes?
We extended our study to a regional comparison in order to speculate about the potential impacts of climate change on the trophic ecology of these large-bodied fish species. Recent evidence indicated that climatic change has the potential to impact plankton and fish within HBL lakes and rivers (Gunn and Snucins 2010; Rühland et al. 2013). Examination of paleolimnological data shows that long-term changes in the algal community have resulted from climatic warming within four HBL lakes (Rühland et al. 2013). Warm temperatures in 2001 led to significant mortalities of brook charr (Salvelinus fontinalis) and white sucker downstream from Hawley Lake in the Sutton River (Gunn and Snucins 2010). However, the potential for more widespread effects on fish in the HBL is not known.
From our findings, it is unlikely that climatic warming would have a dramatic impact on the trophic ecology of northern pike. The regional comparison between HBL and southern lakes indicated that despite climatic, morphometric and chemical differences among regions and lakes, the trophic position and littoral reliance of pike populations from two of the three HBL lakes were similar to Muskrat Lake in the south. Pike can tolerate temperatures up to 29 °C even though they are mesothermal; nevertheless, excessively warm temperatures have been found to affect growth and recruitment to some extent (Casselman 1996, 2002; Casselman and Lewis 1996). Instead, for northern pike, climate change effects will likely be more indirect via alteration of prey availability (Winfield et al. 2008). However, our survey of the three HBL lakes suggests that pike are quite versatile in food use and will likely adapt to changing food sources as their variable trophic position and energy pathways among lakes indicate considerable diet plasticity. Furthermore, the ability of pike to feed on benthic invertebrates and conspecifics (Bry et al. 1992; Venturelli and Tonn 2005) will likely aid in their trophic adaptability as the climate changes.
Based on our limited data, it is unclear whether climatic change will affect walleye trophic dynamics. Even though we found some differences in trophic position and littoral reliance between populations in the northern and southern lakes, we cannot rule out that the differences may not have been attributable to body size differences among populations. It is possible that trophic position and littoral reliance differences between Spruce Lake and the southern lakes were due to size differences of the animals; the larger size of Spruce Lake walleye can explain their higher trophic position compared to Little Lake, and their lower littoral reliance compared to the southern lakes. Walleye are able to readily feed on whatever species are available to them, but as they grow and increase in size they are able to take larger fish as prey because they are gape-limited predators (Scott and Crossman 1973; Graeb et al. 2005). Furthermore, larger fish are able to forage away from the littoral zone, especially when there is niche overlap and interspecific competition with other piscivores such as northern pike (see above discussion).
White suckers too will likely be able to adapt to diet changes under climate change. From past studies based on stomach analyses rather than isotopic inference, suckers are known to exhibit diet plasticity and also the ability to tolerate a relatively wide range of environmental conditions, despite their preference for coolwater habitats (Lyons et al. 2009). Here, we present several lines of evidence to support this inference. First, in agreement with previous studies, we found that suckers are obligatory benthic feeders (Scott and Crossman 1973); hence, they should adapt to changes in benthic food type and availability. Second, this species is ubiquitous across the various climatic zones of North America; a distribution pattern confirmed in this study since they were found in the HBL and southern lakes. Third, the regional comparisons indicate that there were no consistent differences in trophic position and littoral reliance between northern and southern populations. In fact, a couple of notable similarities in populations were evident in lakes that are quite different in morphology; the Hawley Lake white suckers were similar to the southern population in Muskrat Lake, and the Spruce Lake population was similar to the Red Chalk Main population. Although food changes are unlikely to affect suckers, extreme heating, such as the documented 2001 Sutton River event (Gunn and Snucins 2010), could lead to some mortality.
Lake whitefish populations in shallow lakes are probably among the most vulnerable of the northern populations of the HBL. Lake whitefish is known to be a coolwater species with a preference for thermal habitats between 15 and 20 °C during warm summer months, which they are able to access as they vertically migrate through the water column diurnally (Edsall 1999; Gorsky et al. 2012). In the small number of deep lakes, such as Hawley Lake, in HBL, lake whitefish should be able to avoid thermal stress by moving to cooler hypolimnetic waters. However, the real concern with future warming is that there may be a lack of suitable thermal habitat in shallow systems which are typical of the HBL region such as Opinnagau East Lake.
Most of the lakes in the HBL region are very shallow and often large and windswept, such as Opinnagau East Lake, which may facilitate rapid equilibration with air temperature. With climate warming, the coolwater species white sucker and lake whitefish in such lakes may potentially be exposed to an increased incidence of summer episodes of direct thermal stress. However, on the other hand, a shorter ice-covered period with future warming may reduce the incidence of winterkill in such shallow lakes. Given the recent evidence of increasing productivity in HBL lakes (Brazeau et al. 2013; Rühland et al. 2013), fish growth and/or abundance may be expected to generally increase in these systems.
Lake trout are very rare in the HBL, restricted to only Hawley Lake of the HBL lakes in this study and three other comparatively deep lakes (Aquatuk Lake, North Raft Lake and Sutton Lake) within close proximity in the Sutton Ridges area, where an outcrop of the Precambrian Shield protrudes through the sedimentary rock of the Lowlands. In temperate areas, warming air temperatures have not been shown to significantly reduce the coldwater habitat available for lake trout in lakes of sufficient depth to thermally stratify (Keller et al. 2005). In fact, coldwater habitat has sometimes increased with climate warming, due in part to the effects of increased DOC on thermal stratification (Keller et al. 2005, 2007).
Hawley Lake has undergone a dramatic shift in thermal structure with the warmer air temperatures of recent decades in the HBL. In contrast to the very weakly stratified or unstratified thermal conditions observed during the 1970s and 1980s, Hawley Lake was strongly stratified during a particularly intense heating episode in 2001 (Gunn and Snucins 2010). Since then, all measured lake temperature profiles (2009–2012) have shown much stronger thermal stratification than in the 1970s and 1980s (W. Keller, Laurentian University, Sudbury, Ontario; unpublished data) although stratification was not as strong as in 2001.
We speculate that the impacts of climatic change on lake trout in the HBL lakes will likely be dampened considerably because of the morphometry of native lake trout lakes. For example, lake trout in deep Hawley Lake will not likely be directly threatened as the climate warms in the next few decades, but they will occupy a more strongly thermally stratified habitat similar to that of lake trout populations in southern areas. This scenario is also likely for Sutton Lake, a large, deep (Z max > 25 m) lake immediately upstream of Hawley Lake. However, in the other two, shallower, HBL lakes with lake trout populations, North Raft and Aquatuk lakes (Z max < 15 m), surface warming and increased hypolimnetic oxygen depletion with increased productivity could start to reduce the habitat favorable for lake trout (Dillon et al. 2003).
Important next steps for the HBL region
Prior to our study, very little was known about the lake food webs of the HBL region in Northern Ontario. Here, we have characterized fish communities in three HBL lakes and extended our investigation to a regional comparison in order to speculate about potential impacts of climate change on the trophic ecology of select species. We show that energy pathways and trophic position varied among the three HBL lakes which differed in chemistry, morphometry and biological communities; and that most of the large-bodied species exhibit wide variability in their apparent diet and therefore will likely adapt to changing food resources resulting from climatic change. However, we must acknowledge that in focusing on vulnerability of diet changes, we are only addressing a narrow range of potential climate change effects and our inferences are limited by the size of our dataset. Nevertheless, we believe that these are valuable first steps which can be used to direct future research to quantitatively assess whether climate change will impact large-bodied species found within the HBL lakes.
In light of forecasted dramatic climate change in this sensitive region, it is imperative that we characterize the biological communities in more lakes and determine potential impacts on food web dynamics. There is a general lack of data for HBL lakes because access is limited and costly expeditions are required for work in this vast, very remote area. Despite this, we strongly recommend sampling of additional HBL systems for stable isotopes, especially shallow systems such as Opinnagau East Lake which are characteristic of the region. Stable isotopes are ideal for studying food web dynamics in these lakes because we are able to obtain invaluable time-integrated information even from snapshot sampling. Future research should also involve application of habitat availability models (e.g., Dillon et al. 2003; Lester et al. 2004), especially those that consider the potential impact of multiple factors such as hydrology, climate, nutrient inputs, water clarity and land-use changes (Jones et al. 2006). This will allow for more quantitative determination of species-specific changes in optimal habitat within the HBL lakes. Furthermore, combining assessments of food web dynamics and optimal habitat modeling will allow for a more comprehensive evaluation of potential climate change impacts on fish communities in HBL lakes.
References
Abraham KF, McKinnon LM, Jumean Z, Tully SM, Walton LR, Stewart HM (2011) Hudson plains ecozone: status and trends assessment. in canadian biodiversity: ecosystem status and trends 2010, Technical Ecozone Report. Canadian Council of Resource Ministers, Ottawa, ON 445 pp
Andersen M, Jacobsen L, Gronkjaer P, Skov C (2008) Turbidity increases behavioural diversity in northern pike, Esox lucius L., during early summer. Fish Manag Ecol 15:377–383
Anderson C, Cabana G (2007) Estimating the trophic position of aquatic consumers in river food webs using stable nitrogen isotopes. J North Am Benthol Soc 26:273–285
Bozek MA, Haxton TJ, Raabe JK (2011) Walleye and sauger habitat. In: Barton BA (ed) Biology, management, and culture of walleye and sauger. American Fisheries Society, Bethesda, pp 133–197
Brazeau ML, Poulain AJ, Paterson AM, Keller W, Sanei H, Blais JM (2013) Recent changes in mercury deposition and primary productivity inferred from sediments of lakes from the Hudson Bay Lowlands, Ontario, Canada. Environ Pollut 173:52–60
Brown LE, Hannah DM, Milner AM (2007) Vulnerability of alpine stream biodiversity to shrinking glaciers and snowpacks. Glob Change Biol 13:958–966
Bry C, Basset E, Rognon X, Bonamy F (1992) Analysis of sibling cannibalism among pike, Esox lucius, juveniles reared under semi-natural conditions. Environ Biol Fishes 35:75–84
Carl LM, McGuiness F (2006) Lake whitefish and lake herring population structure and niche in ten south-central Ontario lakes. Environ Biol Fish 75:315–323
Carter JL, Schindler DE (2012) Responses of zooplankton populations to four decades of climate warming in lakes of southwestern Alaska. Ecosystems 15:1010–1026
Casselman JM (1996) Age, growth and environmental requirements of pike. In: Craig JF (ed) Pike: biology and exploitation. Chapman & Hall, London, pp 69–101
Casselman JM (2002) Effects of temperature, global extremes, and climate change on year-class production of warmwater, coolwater, and coldwater fishes in the great lakes basin. Am Fish Soc Symp 32:39–60
Casselman JM, Lewis CA (1996) Habitat requirements of northern pike (Esox lucius). Can J Fish Aquat Sci 53(Suppl 1):161–174
Chessman BC (2009) Climatic changes and 13-year trends in stream macroinvertebrate assemblages in New South Wales, Australia. Glob Change Biol 15:2791–2802
Colombo SJ, McKenney DW, Lawrence KM, Gray PA (2007) Climate change projections for Ontario: practical information for policymakers and planners. Climate Change Research Report CCRR—05. Ontario Ministry of Natural Resources
David SM, Sommers KM, Reid RA, Hall RJ, Girard RE (1998) Sampling protocols for the rapid bioassessment of streams and lakes using benthic macroinvertebrates. Ontario Ministry of Environment, ON 65 pp
Dillon PJ, Clark BJ, Molot LA, Evans HE (2003) Predicting the location of optimal habitat boundaries for lake trout (Salvelinus namaycush) in Canadian Shield lakes. Can J Fish Aquat Sci 60:959–970
Edsall TA (1999) The growth-temperature relation of juvenile lake whitefish. Trans Am Fish Soc 128:962–964
Fry B (2006) Stable isotope ecology. Springer Science+Business Media, New York
Gorsky D, Zydlewski J, Basley D (2012) Characterizing seasonal habitat use and diel vertical activity of lake whitefish in Clear Lake, Maine, as determined with acoustic telemetry. Trans Am Fish Soc 141:761–771
Graeb BDS, Galarowicz T, Wahl DH, Dettmers JM, Simpson MJ (2005) Foraging behavior, morphology, and life history variation determine the ontogeny of piscivory in two closely related predators. Can J Fish Aquat Sci 62:2010–2020
Gunn J, Snucins E (2010) Brook charr mortalities during extreme temperature events in Sutton River, Hudson Bay Lowlands, Canada. Hydrobiologia 650:79–84
Jackson AL, Inger R, Parnell AC, Bearhop S (2011) Comparing isotopic niche widths among and within communities: SIBER—Stable Isotope Bayesian Ellipses in R. J Anim Ecol 80:595–602
Jensen OP, Benson BJ, Magnuson JJ, Card VM, Futter MN, Soranno PA, Stewart KM (2007) Spatial analysis of ice phenology trends across the Laurentian Great Lakes region during a recent warming period. Limnol Oceanogr 52:2013–2026
Jones ML, Shuter BJ, Zhao Y, Stockwell JD (2006) Forecasting effects of climate change on Great Lakes Fisheries: models that link habitat supply to population dynamics can help. Can J Fish Aquat Sci 63:457–468
Keller W (2007) Implications of climate warming for Boreal Shield lakes: a review and synthesis. Environ Rev 5:99–112
Keller W, Heneberry J, Leduc J (2005) Linkages between weather, dissolved organic carbon and coldwater habitat in a Boreal Shield lake recovering from acidification. Can J Fish Aquat Sci 62:340–346
Keller W, Paterson AM, Somers KM, Dillon PJ, Heneberry J, Ford A (2008) Relationships between dissolved organic carbon concentrations, weather, and acidification in small Boreal Shield lakes. Can J Fish Aquat Sci 65:786–795
Keller W, Paterson A, Jones C, Heneberry J, Winter J, Johnston T, Haslam L, Gunn J, Houle J, White M, MacPhee S, Smol J, Rühland K, Blais J, Poulain A, Brazeau M, Wolfe B, Wilson H, Mueller D, Persaud A, Dillon P, Finkelstein S (2011) Progress Report- Aquatic ecosystem studies in the Hawley Lake/Sutton River area of the Hudson Bay Lowlands, 2009–2010. Cooperative Freshwater Ecology Unit, Laurentian University, ON 50 pp
Keller W, MacPhee S, Sarrazin-Delay C, Gunn J, Smol J, Rühland K, Hargan K, Jeziorski A, Hadley K, Blais J, Poulain A, Brazeau M, Wolfe B, White H, Mueller D, Koponen L, Persaud A, Dillon P, Finkelstein S, Bunbury J, Packalen M, Paterson A, Jones C, Heneberry J, Winter J, Johnston T, Haslam L, and Houle J (2013) Second Progress Report—Aquatic ecosystem studies in the Hawley Lake/Sutton River area of the Hudson Bay Lowlands, 2009–2012. Cooperative Freshwater Ecology Unit, Laurentian University, ON 52 pp
Kratina P, Greig HS, Thompson PL, Carvalho-Pereira TSA, Shurin JB (2012) Warming modifies trophic cascades and eutrophication in experimental freshwater communities. Ecology 93:1421–1430
Layman CA, Quattrochi JP, Peyer CM, Allgeier JE (2007) Niche width collapse in a resilient top predator following ecosystem fragmentation. Ecol Lett 10:937–944
Lester NP, Dextrase AJ, Kushneriuk RS, Rawson MR, Ryan PA (2004) Light and temperature: key factors affecting walleye abundance and production. Trans Am Fish Soc 133:588–605
Luek A, Morgan GE, Wissel B, Gunn JM, Ramcharan CW (2013) Impaired littoral energy pathways cause a shift to pelagic resources by fish in recovering lake food webs. Ecol Freshw Fish 22:348–360
Lyons J, Zorn T, Stewart J, Seelbach P, Wehrly K, Wang L (2009) Defining and characterizing coolwater streams and their fish assemblages in Michigan and Wisconsin, USA. North Am J Fish Manag 29:1130–1151
Macrae ML, Bello RL, Molot LA (2004) Long-term carbon storage and hydrological control of CO2 exchange in tundra ponds in the Hudson Bay Lowland. Hydrol Process 18:2051–2069
McKenney DW, Pedlar JH, Lawrence K, Gray P, Colombo SJ, Crins WJ (2010) Current and projected future climatic conditions for ecoregions and selected natural heritage areas in Ontario. Climate Change Research Report CCCR-16. Ontario Ministry of Natural Resources
Mooij WM, Hulsmann S, De Senerpont Domis LN, Nolet BA, Bodelier PLE, Boers PCM, Pires LMD, Gons HJ, Ibelings BW, Noordhuis R, Portielje R, Wolfstein K, Lammens EHRR (2005) The impact of climate change on lakes in the Netherlands: a review. Aquat Ecol 39:381–400
Morgan GE, Snucins E (2005) Manual of instructions and provincial biodiversity Benchmark values NORDIC index netting. Ontario Ministry of Natural Resources
Moss B (2010) Climate change, nutrient pollution and the bargain of Dr. Fautus. Freshw Biol 55:175–187
Ontario Ministry of the Environment (1983) Handbook of analytical methods for environmental samples. Volume 1 & 2. Ontario Ministry of the Environment, ON 686 pp
Organization for Economic Co-operation and Development (1982) Eutrophication of waters: monitoring, assessment and control. Organization for economic and co-operative development, Paris, France
Parnell A, Jackson AL (2013) SIAR: Stable isotope analysis in R. R package version 4.2. http://CRAN.R-project.org/package=siar
Peterson BJ, Fry B (1987) Stable isotopes in ecosystem studies. Annu Rev Ecol Syst 18:293–320
Post DM (2002) Using stable isotopes to estimate trophic position: models, methods and assumptions. Ecology 83:703–718
R Core Team (2013) R: a language and environment for statistical computing. Vienna, Asutria: R Foundation for Statistical Computing, Vienna, Austria
Rühland KM, Paterson AM, Keller W, Michelutti N, Smol JP (2013) Global warming triggers the loss of a key Arctic refugium. Proc R Soc B 280:1887–1895
Ryder RA (1977) Effects of ambient light variations on behavior of yearling, subadult, and adult wall-eyes (Stizostedion vitreum vitreum). J Fish Res Board Can 34:1481–1491
Sandstrom S, Rawson M, Lester N (2008) Manual for broad-scale fish community monitoring; using large mesh and small mesh gillnets. Peterborough, Ontario Ministry of Natural Resources, ON 32 pp
Scott WB, Crossman EJ (1973) Freshwater fishes of Canada. Bulletin 184. Fisheries Research Board of Canada, 966 pp
Vander Zanden MJ, Vadeboncoeur Y (2002) Fishes as integrators of benthic and pelagic food webs in lakes. Ecology 83:2152–2161
Venturelli PA, Tonn WM (2005) Invertivory by northern pike (Esox lucius) structures communities of littoral macroinvertebrates in small boreal lakes. J North Am Benthol Soc 24:904–918
Whitehead PG, Wilby RL, Battarbee RW, Kernan M, Wade AJ (2009) A review of the potential impacts of climate change on surface water quality. Hydrol Sci J 54:101–123
Winfield IJ, Ben James J, Fletcher JM (2008) Northern pike (Esox lucius) in a warming lake: changes in population size and individual condition in relation to prey abundance. Hydrobiologia 601:29–40
Acknowledgments
This work was supported by the Ontario Ministry of the Environment through the Climate Change and Multiple Stressor Research Program at Laurentian University. Albert Chookomolin and Gilbert Chookomolin assisted with the field studies in the HBL. Lee Haslam and Jason Houle collected the fish samples from the HBL and Muskoka-Haliburton Lakes. Jeff Amos of MNR provided the fish community data for Spruce Lake. Matthew Bond and Cam McCauley from the Ministry of Natural Resources (MNR) along with numerous field and laboratory technicians from Trent University assisted with sample collection and processing for the Kawartha-Renfrew Lakes. We also thank three anonymous reviewers for their comments that helped to improve our paper.
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Persaud, A., Luek, A., Keller, W. et al. Trophic dynamics of several fish species in lakes of a climatically sensitive region, the Hudson Bay Lowlands. Polar Biol 38, 651–664 (2015). https://doi.org/10.1007/s00300-014-1628-1
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DOI: https://doi.org/10.1007/s00300-014-1628-1