Abstract
The cytoprotective ATP receptor P2Y11 is upregulated during M2 macrophage differentiation and contributes to the anti-inflammatory properties of this macrophage subset. Here, we studied P2Y11-induced reprogramming of human M2 macrophages at the level of mRNA and protein expression. Upregulation of IL-1 receptor (IL-1R) and its known downstream effectors VEGF, CCL20 and SOCS3 as well as downregulation of the ATP-degrading ecto-ATPase CD39 emerged as hallmarks of P2Y11 activation. The anti-inflammatory signature of the P2Y11 transcriptome was further characterized by the downregulation of P2RX7, toll-like receptors and inflammasome components. P2Y11-induced IL-1R upregulation formed the basis for reinforced IL-1 responsiveness of activated M2 macrophages, as IL-1α and IL-1ß each enhanced P2Y11-induced secretion of VEGF and CCL20 as well as the previously reported shedding of soluble tumor necrosis factor receptor 2 (sTNFR2). Raising intracellular cyclic AMP (cAMP) in M2 macrophages through phosphodiesterase 4 inhibition enhanced P2Y11-driven responses. The cAMP-binding effector, exchange protein activated by cAMP 1 (Epac1), which is known to induce SOCS3, differentially regulated the P2Y11/IL-1R response because pharmacological Epac1 inhibition enhanced sTNFR2 and CCL20 release, but had no effect on VEGF secretion. In addition to cAMP, calcium and protein kinase C participated in P2Y11 signaling. Our study reveals how P2Y11 harnesses canonical and IL-1R signaling to promote an anti-inflammatory and pro-angiogenic switch of human M2 macrophages, which may be controlled in part by an Epac1-SOCS3 axis.
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Introduction
The concentration of adenosine triphosphate (ATP) in interstitial fluid of undisturbed tissues remains in the nanomolar range. However, stressed cells release ATP in a controlled or lytic manner, raising the levels of extracellular ATP to low or even high micromolar concentrations. For instance during infection, microbial products stimulate toll-like receptors (TLRs) to induce enhanced ATP release [1,2,3]. Extracellular ATP can now act as a damage-associated molecular pattern (DAMP) and cause the coordinate activation of ATP-responsive P2 purinergic receptors on immune cells [4, 5], which translate the ATP alarm into either pro-inflammatory, cytolytic (e.g. P2X7) or immunomodulatory, cytoprotective responses (e.g. P2Y11).
P2Y11 is an atypical representative of the P2Y family of G protein-coupled receptors (GPCRs), as it can couple to both, Gq and Gs proteins, and does not exist in rodents [6, 7]. Among immune cells, P2Y11 has been studied mainly in dendritic cells, macrophages and in T cells [8,9,10,11]. We, and others, have recently shown that P2Y11 mRNA and protein expression substantially increase during monocyte-to-macrophage differentiation [8, 12].
The lack of rodent models and the limited availability of P2Y11-specific research tools has long slowed down the systematic examination of P2Y11 signaling [13, 14]. In a recent study, we used transcriptome profiling of recombinant P2Y11 expressed in a P2R-deficient astrocytoma cell line to examine P2Y11 downstream signaling [15]. We found that P2Y11 promoted the release (shedding) of soluble TNF receptors through TNF-α converting enzyme (TACE), also known as ADAM17 (a disintegrin and metalloprotease 17), both in astrocytoma cells (sTNFR1) and in human M2 macrophages (sTNFR2). Moreover, we found that P2Y11 blocks TLR4-driven TNF-α secretion in M2 macrophages, altogether establishing P2Y11 as a target for anti-inflammatory strategies [15].
Gq protein coupling of P2Y11 results in phospholipase Cß-catalyzed production of second messengers, which mobilize Ca2+ (via inositol trisphosphate, IP3) and activate protein kinase C (via diacylglycerol, DAG) [16]. By coupling to Gs, P2Y11 can activate adenylyl cyclase (AC) and thus raise the intracellular levels of cAMP, which has potent anti-inflammatory activity [17]. Accordingly, we previously found that inhibition of cAMP degradation by blockade of PDE4 further enhanced the P2Y11-driven release of sTNFR2 from macrophages [15]. Intracellular cAMP acts via at least three mechanisms to modulate immune responses [17]: protein kinase A (PKA), also known as cAMP-dependent protein kinase, the two isoforms of exchange protein directly activated by cAMP, Epac1 and Epac2, as well as cyclic nucleotide-gated (CNG) ion channels in distinct cell types.
In the present work, we wanted to examine P2Y11 canonical and IL-1R signaling as well as the downstream effects that determine the functional phenotype of human M2 macrophages. We have established the first transcriptional profile of native P2Y11 signaling in its natural environment, also taking into account the levels of intracellular cyclic AMP. We not only provide evidence for P2Y11/IL-1R crosstalk in human M2 macrophages but also identify several novel P2Y11 target genes. In addition, the cAMP effector Epac1, a well-established inducer of suppressor of cytokine signaling 3 (SOCS3) [18, 19], turns out to be a regulator of P2Y11/IL-1R signaling. Collectively, the observed signature of response is consistent with a P2Y11-driven polarization of anti-inflammatory and pro-angiogenic M2 macrophages.
Materials and methods
Reagents
The slowly hydrolyzable ATP analog ATPγS [7, 20] (Sigma Aldrich, St. Louis, MO, USA) as well as the suramin analog NF340 [7, 10, 13, 21] (Santa Cruz, Dallas, TX, USA) served as P2Y11 receptor agonist (20 µM) and antagonist (20 µM), respectively. Further reagents utilized in this study include the PDE4-selective inhibitor rolipram (10 µM) (Sigma-Aldrich), BAPTA-AM (10 µM) (Sigma-Aldrich), calphostin C (250 nM) (Tocris), recombinant IL-1α and IL-1β (0.5–2 ng·ml−1) (R&D Systems, Minneapolis, MN, USA), the TACE/ADAM17 inhibitor TAPI-1 (20 µM) (Tocris), the selective inhibitor of Epac1 (R)-CE3F4 (20 µM) (Tocris), and the humanized anti-VEGF monoclonal antibody bevacizumab (0.5 µg ml−1) (Selleckchem, Houston, TX, USA).
Isolation of human monocytes and generation of M2 macrophages
Buffy coats from randomly selected anonymous donors were provided by the Central Institute for Blood Transfusion (Innsbruck, Austria) after written informed consent. Inclusion of healthy donors in the present study was approved by the local Institutional Review board (ethical committee number: 1087/2018).
PBMCs were isolated from these buffy coats by density gradient centrifugation (Lymphoprep; Stem Cell Technologies, Vancouver, Canada). Subsequently, monocytes were isolated from PBMCs by positive selection using CD14 microbeads (human; 130-050-201, Miltenyi Biotec, Bergisch Gladbach, Germany) and LS columns (130-042-401, Miltenyi Biotec). Freshly isolated monocytes were differentiated toward M2 macrophages for 6 days in RPMI1640 (Lonza, Basel, Switzerland) supplemented with 20% FBS (HyClone, Logan, UT, USA), 1% GlutaMAX (100×; Gibco/Thermo Fisher Scientific, Waltham, MA, USA), 10 mM HEPES (1 M), 1 mM sodium pyruvate (100 mM), 1% nonessential amino acid mixture (NEAA; 100×) and 1% Pen/Strep (10.000 U ml−1; all from Lonza) in the presence of 50 ng·ml−1 macrophage colony-stimulating factor (M-CSF; Miltenyi Biotec). Fresh medium containing 50 ng·ml−1 M-CSF was added on day 2 and day 5.
Stimulation of native P2Y11 in human M2 macrophages
On day 6, fully differentiated M2 macrophages were harvested, washed and seeded in 100 µl RPMI1640 supplemented with 5% FBS, 1% GlutaMAX, 10 mM HEPES, 1 mM sodium pyruvate, 1% NEAA and 1% Pen/Strep in 96-well plates (Corning/Costar, New York, USA) at a density of 5 × 104 cells per well. Cells were stimulated in duplicates with the P2Y11 receptor agonist ATPγS (20 µM) in the presence or absence of antagonists/inhibitors and/or recombinant cytokines for 24 h. Microscopic inspection and flow cytometry-based eFluor780 exclusion was used to exclude toxicity of the inhibitors used. Supernatants were harvested and cryopreserved at − 80 °C.
Stimulation of ectopic P2Y11 in human astrocytoma cells
Ectopic P2Y11 receptor was studied in the recombinant cell line ES-293-A (Perkin Elmer). This cell line originates from the glioma cell line 1321N1, a grade II brain astrocytoma naturally devoid of functional P2 receptors, which has been stably transfected with human P2Y11 receptor-coding cDNA. By using CRISPR/Cas9-mediated knockout, we previously generated a corresponding control cell line lacking P2Y11 receptors (P2RY11-KO) [15]. Both the P2Y11-expressing cell line (P2RY11) and the knockout control cell line (P2RY11-KO) were regularly sorted on a BD FACSAria at the local FACS core facility to select for presence or absence of P2Y11, respectively.
Upon confluence, cells were harvested, washed and 1 × 105 cells were seeded in 400 µl Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) FBS, 1 mM sodium pyruvate, 2 mM L-alanyl-L-glutamine (Glutamax), 100 units ml−1 of penicillin, and 100 µg ml−1 of streptomycin. At this stage, FBS at 10% was required to facilitate cell attachment. P2Y11 stimulation (24 h) occurred in the presence of 1% FBS and in the absence of G418 (geneticin), which was otherwise used at 400 µg ml−1 to select for stable transfectants as well as knockouts.
Flow cytometry
Cellular antigens were stained with fluorochrome-conjugated monoclonal (mouse) or polyclonal (rabbit, goat) antibodies. Isotype-matched control stainings were performed in parallel using the same concentration to determine unspecific background signals. First, cells were harvested, washed and stained with fixable viability dye eFluor 780 (eBioscience/Thermo Fisher Scientific) to enable dead cell discrimination. Following another washing step, cells were stained for 30 min at 4 °C in the dark in PBS (Lonza) containing 0.5% FBS and 50 µg ml−1 human IgG (Octapharma, Lachen, Switzerland) to block Fcγ receptors. In order to access intracellular antigens, cells were fixed with intracellular fixation buffer (Invitrogen, Waltham, MA, USA) for 30 min at room temperature in the dark after dead cell staining. Following two washing steps, cells were stained in permeabilization buffer (Nordic-MUbio, Susteren, Netherlands) for 30 min at room temperature in the dark. The following antibodies were used: rabbit polyclonal IgG antihuman P2Y11 receptor (bs-12071R-A-488; Bioss, Woburn, MA, USA), mouse monoclonal IgG2b antihuman CD14 (clone MϕP9, 345787-APC; BD Biosciences, Franklin Lakes, NJ, USA), mouse monoclonal IgG1 antihuman CD163 (clone GHI/61, 556018-PE; BD Biosciences), goat polyclonal IgG antihuman IL-1R1 (FAB269P-PE; R&D Systems) and mouse monoclonal IgG2b antihuman CD39 (clone TU66, 561444-FITC; BD Biosciences).
Acquisition and analysis of samples was performed on a FACSCanto II flow cytometer equipped with FACS Diva 6.1.2 software. The data were processed using FlowJo V7.2.5 software (BD Biosciences) by applying dead cell and doublet discrimination.
Transcriptome analysis
Gene expression was examined using the NanoString’s nCounter analysis system as described previously [15]. Fully differentiated M2 macrophages were seeded in 1 ml RPMI1640 supplemented with 5% FBS, 1% GlutaMAX, 10 mM HEPES, 1 mM sodium pyruvate, 1% NEAA and 1% Pen/Strep in 12-well plates (Corning/Costar) with a density of 5 × 105 cells per well. Cells were stimulated in duplicates with the P2Y11 receptor agonist ATPγS (20 µM) in the presence or absence of the P2Y11 receptor antagonist NF340 (20 µM) and/or the PDE4-selective inhibitor rolipram (10 µM). After 6 h, cells were harvested and total RNA was isolated from cell pellets using the RNeasy Plus Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. Cell supernatants were analyzed for the presence or absence of sTNFR2 to check the successful stimulation or inhibition of the P2Y11 receptor. Total RNA samples were then quality checked on an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) with an RNA 6000 Nano LabChip and samples with RNA integrity factors above 8.0 were used for further analysis. 50 ng of total RNA were used for hybridization reaction with the nCounter Host Response Panel Kit (human) according to supplier’s instructions (NanoString Technologies, Seattle, WA USA). Samples were processed at the Core Facility Molecular Biology at the Centre of Medical Research at the Medical University of Graz. The automated NanoString platform is based on fluorescent barcodes and digital readout allowing for the non-amplified measurement of 773 protein-coding mRNA sequences within one sample. The NanoString platform has been shown to be comparable with other technologies, with considerable sensitivity, reproducibility and technical robustness (https://www.nanostring.com/scientific-content/publications).
Raw data preprocessing and normalization were performed using nSolver 2.5 Software (NanoString Technologies, Seattle, WA USA) according to standard procedures (background subtraction, positive and negative controls normalization). Subsequently, gene counts were normalized to the geometric mean of the 40 reference genes. Normalized data were uploaded to Partek Genomic Suite Software v6.6 (Partek Inc, St Louis, MO). For statistical analysis, one-way ANOVA was calculated and genes with p < 0.05 and fold change of at least 1.5 were considered as differentially regulated.
Cytokine measurements
Cytokine levels in cell culture supernatants were assessed by cytometric bead arrays (CBA) from BD using human CBA Flex Sets for VEGF and sTNFR2 according to the manufacturer’s instructions. Supernatants were analyzed on a FACSCanto II flow cytometer and FCAP Array 1.0.1 software (BD).
The levels of CCL20 (MIP-3α) in cell culture supernatants were determined by ELISA using the Human MIP-3 alpha ELISA Kit (RayBiotech, Peachtree Corners, GA, USA) or the Human CCL20/MIP-3 alpha DuoSet (R&D Systems) according to the manufacturer’s instructions. Measurements were carried out on an Elx800 universal microplate reader (BioTek Instruments/Agilent, Winooski, VT, USA) and results were processed using the Gen5 3.09 data analysis software (BioTek Instruments/Agilent).
Data and statistical analysis
The data are presented as mean values ± SD. Sample sizes and experimental replicates are indicated in figure legends. Statistical analyses were conducted with the GraphPad Prism software (version 9). Statistical significance was determined by ordinary one-way ANOVA including Bonferroni correction. An output of p < 0.05 was accepted as significantly different in all tests. Significance levels are: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Results
Transcriptome profiling of native P2Y11 in human M2 macrophages reveals a strong signature of an anti-inflammatory and proangiogenic P2Y11/IL-1R crosstalk
To examine P2Y11 downstream signaling events, we set out to establish the transcriptional profile of P2Y11 activation. Although our previous study focused on ectopic P2Y11 in a recombinant cell system [15], we here investigated native P2Y11 in its natural environment. Human monocytes upregulate P2Y11 along with the M2 marker CD163 during macrophage differentiation induced by M-CSF (Fig. 1A, B) [8] and release sTNFR2 in response to triggering of P2Y11 with its specific agonist ATPγS (20 µM) (Fig. 1C) [15]. NF340 (20 µM), which is currently the most useful antagonist at the P2Y11 receptor [10], effectively prevented P2Y11-induced and rolipram-enhanced sTNFR2 release. Rolipram inhibits PDE4 and thus increases the levels of intracellular cAMP [22]. Using this well-defined experimental cell system [15], we analyzed gene expression with NanoString technology and found a distinct pattern of P2Y11/cAMP-driven reprogramming in human M2 macrophages (Fig. 1D). Activation of native P2Y11 caused upregulation of the IL-1R family members IL1R1, IL1RAP and IL1R2 [23] in a manner very similar to that previously observed with ectopic P2Y11 [15]. IL1RN, the gene encoding IL-1R antagonist, was also activated. The activating IL-1R is a complex of two chains, the IL-1α/IL-1ß-binding chain IL-1R1 (encoded by IL1R1) and the accessory protein IL-1R3 (encoded by IL1RAP), which is responsible for signaling [23].
NLRP3 inflammasome activation in macrophages requires two signals [24]. The first signal, also known as priming signal, can be provided by microbial or endogenous molecules that trigger toll-like receptors. The second signal, causing inflammasome activation, can be delivered by ATP-activated P2X7. Intriguingly, both priming (toll-like receptors) and secondary signals (P2RX7) were downregulated upon P2Y11 activation along with NLRP3, CASP1 and PYCARD, which encode the NLRP3 inflammasome components NLRP3, pro-caspase-1 and the adapter protein PYCARD (ASC) (Table 1). Moreover, in support of an efficient activation of P2Y11, ENTPD1, the gene encoding the ecto-ATPase CD39, was downregulated.
The P2Y11 transcriptome also contained the signature of an angiogenic response. In addition to the pro-angiogenic chemokine CXCL8 (IL8), which previously emerged from the transcriptional profile of ectopic P2Y11 [15], VEGFA and CCL20, encoding vascular endothelial growth factor and chemokine (C−C motif) ligand 20, respectively, were also found to be P2Y11 target genes in M2 macrophages (Fig. 1D). The observed P2Y11-driven anti-inflammatory and pro-angiogenic transcriptional responses were even more pronounced, when PDE4-mediated cAMP degradation was inhibited with rolipram. Finally, SOCS3, which encodes suppressor of cytokine signaling 3, was also upregulated by P2Y11 signaling (Fig. 1D). This was of particular interest, because SOCS3 is a known feedback regulator of IL-1 signaling [25] and an anti-inflammatory target gene of the cAMP-binding effector Epac1 [18, 19].
NanoString-based gene expression analysis reveals mRNA copy numbers for each individual gene and by using a set of negative control probes, the general gene expression threshold can be determined. We thus found that IL1R2, CCL20 and SOCS3 genes were silent in human M2 macrophages but were activated upon P2Y11 activation (not shown).
To validate selected candidates emerging from the NanoString analysis, we performed flow cytometric analyses and cytokine bead arrays. Upregulation of IL-1R1 expression on the surface of human M2 macrophages in response to P2Y11 activation with ATPγS is shown in Fig. 2A, B. P2Y11 stimulation in the presence of rolipram caused a significant further increase of IL-1R1 expression. NF340 effectively prevented both, P2Y11-driven and rolipram-boosted upregulation of IL-1R1 (Fig. 2A, B). Conversely, ENTPD1-encoded CD39, which acts as an ecto-ATPase on the surface of macrophages and other cell types to degrade extracellular ATP [26], was downregulated during P2Y11 signaling (Fig. 2A/C), thus facilitating the prolonged activation of P2Y11 by its physiological agonist ATP. VEGF secretion could also be validated (Fig. 2D) and showed a pattern almost identical to that of sTNFR2 release (Fig. 1C).
ATPγS-induced secretory responses are specific to P2Y11 and additionally depend on Ca2+ and protein kinase C
ATPγS is a selective P2Y11 receptor agonist [7]. According to the IUPHAR/BPS Guide to Pharmacology [27], ATPγS is primarily considered a full agonist at the P2Y11 receptor. In some recombinant cell systems, it may however also act as a partial agonist at P2Y1 [28] and as a full agonist at P2Y13 [29]. We therefore performed additional experiments using well-established antagonists of P2Y1 (MRS 2500) [30] and P2Y13 (MRS 2211) [31]. Importantly, both had little effect on ATPγS-induced and rolipram-enhanced VEGF secretion and sTNFR2 release (Fig. S1). In contrast, NF340 effectively prevented these responses. Therefore, we considered ATPγS a bona fide, potent, and selective P2Y11 agonist in human M2 macrophages.
The observed stimulatory effects of the PDE4 inhibitor rolipram clearly implicated cAMP signaling in sTNFR2 release, VEGF secretion and IL-1R upregulation but also raised the question of whether cAMP elevation is sufficient to induce these responses. To address this point, we directly activated AC with forsoklin. Forskolin had a very modest stimulatory effect on VEGF secretion and sTNFR2 release, which was enhanced in the presence of rolipram (Fig. 3A). However, forskolin was much less effective compared to ATPγS, suggesting that ATPγS-activated P2Y11 couples to additional signaling pathways. Rolipram alone had no effect. In contrast to VEGF secretion and sTNFR2 release, forskolin failed to upregulate IL-1R even in the presence of rolipram (Fig. 3A), indicating that cAMP elevation is not sufficient to increase IL-1R expression.
SQ22536 is probably the most widely used AC inhibitor in intact cell studies [32]. However, its potency is rather low. We tested SQ22536 in concentrations of up to 100 µM but did not observe significant inhibition of sTNFR2 release and VEGF secretion. We did not test higher concentrations as they might cause off-target effects [32].
The differential coupling of P2Y11 to G proteins not only leads to the elevation of intracellular cAMP but may also cause activation of Ca2+ and protein kinase C (PKC) signaling [16]. To investigate these second messengers in P2Y11-driven responses, we used the Ca2+ chelator BAPTA-AM [33] and the PKC inhibitor calphostin C [34]. BAPTA-AM significantly inhibited ATPγS-induced and rolipram-enhanced sTNFR2 release and VEGF secretion (Fig. 3B). Calphostin C also inhibited both, sTNFR2 release and VEGF secretion, but only sTNFR2 inhibition was significant.
In addition to cAMP-elevating agents such as rolipram (Figs. 1 and 2) or prostaglandin E2 [35], IL-1R expression is known to be stimulated by IL-1, Ca2+ and PKC [35,36,37]. Accordingly, BAPTA-AM significantly suppressed ATPγS-induced and rolipram-enhanced IL-1R upregulation (Fig. 3B). Calphostin C also showed an inhibitory effect, which was however less potent and not significant. Collectively, our data clearly implicate cAMP and Ca2+ in ATPγS-induced and rolipram-enhanced P2Y11 signaling in human M2 macrophages.
P2Y11-induced IL-1R upregulation translates into increased IL-1 responsiveness of human M2 macrophages: PDE4 inhibition potentiates the secretory response
Next, we investigated the regulation of P2Y11-induced VEGF secretion by exogenous IL-1 and by intracellular cAMP. The release of sTNFR2 served as a control in these experiments. IL-1α and IL-1ß moderately enhanced sTNFR2 release from M2 macrophages in a dose-dependent manner (Fig. S2). However, the induction of VEGF secretion by IL-1 cytokines was ineffective (Fig. 4). In contrast, the concurrent stimulation of P2Y11 and IL-1R resulted in the synergistic upregulation of both, sTNFR2 release and VEGF secretion (Fig. S2 and Fig. 4). The synergistic enhancement of the IL-1R—driven secretory response by ATPγS could effectively be inhibited with the P2Y11 antagonist NF340. Inducing the accumulation of intracellular cAMP by PDE4 blockade with rolipram potentiated the P2Y11/IL-1R—driven secretion of sTNFR2 and VEGF (Fig. S2 and Fig. 4C, D). The synergy of the P2Y11/IL-1R crosstalk was more pronounced for VEGF secretion than for sTNFR2 release.
The late stage accumulation of sTNFR2 and VEGF at 24 h might be considered part of an anti-inflammatory process that occurs in response to prolonged IL-1R activation and may therefore represent secondary, negative feedback signaling to control the initial pro-inflammatory IL-1R response. To address this point, we have performed kinetic analyses of sTNFR2 release and VEGF secretion (6, 12, and 24 h). P2Y11 signaling induced by ATPγS alone stimulated sTNFR2 release and VEGF secretion, which were clearly detectable at 6 h and continued to increase at 12 h but no longer at 24 h (Fig. S3). Although rolipram had little or no effect on ATPγS-induced secretory responses at 6 h, it modestly increased sTNR2 levels at 12 h and 24 h, and more effectively, the levels of VEGF at 24 h. IL-1 co-stimulation had relatively little effect at 6 h, but strongly enhanced the ATPγS-driven release of sTNFR2 as well as the secretion of VEGF, both at 12 h and at 24 h. Combined P2Y11 co-stimulation with rolipram and IL-1 resulted in the highest levels of sTNFR2 and VEGF, however, 24 h were required to express the full co-stimulatory effect.
Taken together, these observations suggested that the observed sTNFR2 release and VEGF secretion is the result of an immediate, direct response and not secondary to strong pro-inflammatory IL-1R signaling. Early P2Y11 canonical signaling causes IL-1R upregulation (Figs. 1D and 2A) and thus facilitates IL-1 co-stimulation, which, however, depends on protracted signaling and possibly also on enhanced gene expression [38].
VEGF has been implicated in the stimulation of ADAM17-dependent shedding of cell surface receptors [39], raising the possibility that P2Y11-induced VEGF promotes ADAM17-dependent sTNFR2 release. To test this, we used bevacizumab, a humanized anti-VEGF monoclonal antibody. Bevacizumab effectively neutralized P2Y11-induced VEGF, however, this had no effect on P2Y11-induced sTNFR2 release (Fig. S4), excluding the possibility that VEGF stimulates ADAM17 in our setting.
The cAMP binding effector protein Epac1 controls P2Y11-induced sTNFR2 release
The strong P2Y11-induced and rolipram-enhanced upregulation of SOCS3 (Fig. 5A), which is a target of Epac1 (Fig. 5B) [18], suggested an involvement of the cAMP-binding effector protein Epac1. To test this possibility, we used the (R)-enantiomer of CE3F4, which is a preferential inhibitor of Epac1 [40]. Epac1 inhibition with (R)-CE3F4 enhanced the P2Y11-mediated release of sTNFR2 (Fig. 5C) but had no effect on VEGF secretion (Fig. 5D). The release of sTNFR2 induced by ATPγS alone or ATPγS plus either IL-1 or rolipram was significantly enhanced by Epac1 inhibitor (R)-CE3F4. In contrast, the strong sTNFR2 release triggered by ATPγS plus IL-1 and rolipram appeared to approach saturation as (R)-CE3F4 was much less effective in enhancing it (Fig. 5C).
Next, we examined the role of ADAM17 in the P2Y11-driven and (R)-CE3F4-enhanced secretory response. Both, P2Y11 inhibitor NF340 (20 µM) and ADAM17 inhibitor TAPI-1 (20 µM) suppressed the ATPγS/IL-1-induced and (R)-CE3F4-enhanced release of sTNFR2 from M2 macrophages (Fig. 6). However, although NF340 also prevented the ATPγS/IL-1-induced secretion of VEGF, TAPI-1 as expected had no effect on this response (Fig. S5).
P2Y11/IL-1R crosstalk activates CCL20: PDE4 inhibition potentiates and Epac1 regulates the secretory response
We have also performed mRNA profiling of recombinant P2Y11 in a side-by-side stimulation of astrocytoma cells with either P2RY11 overexpression (P2RY11) or P2RY11 knockdown (P2RY11-KO) [15]. ATPγS induced strong upregulation of CCL20 mRNA in P2RY11 cells but not in P2RY11-KO cells (Fig. 7A). In fact, CCL20 was among the most highly activated genes in response to ectopic P2Y11 stimulation in astrocytoma cells. However, despite the much stronger signature of IL-1 signaling in P2RY11 cells [15], we found that ATPγS alone failed to stimulate CCL20 production and that exogenous IL-1α or IL-1ß were still required (Fig. 7B). Both, P2RY11 and P2RY11-KO cells produced low levels of CCL20 in response to increasing doses of IL-1. However, the synergistic upregulation with ATPγS was only observed in P2RY11 cells. Moreover, ATPγS/IL-1-driven CCL20 production in P2RY11 cells was effectively suppressed by NF340.
In human M2 macrophages, ATPγS also induced strong upregulation of CCL20 mRNA and rolipram enhanced this response (Fig. 8A). Both, ATPγS-induced and rolipram-enhanced CCL20 gene activation could be inhibited with NF340. It is important to note that only CCL20 was upregulated among the 24 CCL chemokines analyzed (Fig. 8B), implying a strong selectivity in P2Y11-induced CCL chemokine activation. The synergy between P2Y11 and IL-1R was extreme for CCL20 secretion. ATPγS or IL-1 alone failed to induce CCL20 secretion (Fig. 8C, D). The combination of ATPγS and IL-1 was required to induce low-level secretion of CCL20. Raising cAMP through PDE4 suppression appeared to be the critical step as it caused robust upregulation of P2Y11/IL-1R—driven CCL20 secretion (Fig. 8C, D).
Similar to VEGF secretion and sTNFR2 release, CCL20 production not only depended on cAMP but also on Ca2+ and PKC signaling (Fig. 9A). While BAPTA-AM partially inhibited CCL20 production driven by ATPγS/IL-1 and ATPγS/IL-1/rolipram, Calphostin C abrogated the response almost completely, confirming the particularly strong PKC dependence of CCL20 biosynthesis [41] and suggesting that Ca2+ mainly serves to support PKC activation.
Although all pathways of canonical signaling (cAMP, Ca2+ and PKC) contributed to P2Y11/IL-1R-mediated CCL20 production (Fig. 9A), Epac1 was found to regulate the response. CCL20 secretion stimulated by ATPγS plus IL-1 was strongly enhanced by Epac1 inhibitor (R)-CE3F4 (≈100% increase) (Fig. 9B). However, in contrast to sTNFR2 release, the rolipram-boosted CCL20 secretion could no longer be increased by Epac1 inhibition.
Discussion
We, and others, have previously shown that human P2Y11 is upregulated during M-CSF driven monocyte-to-macrophage differentiation [8, 12]. P2Y11 equips these cells with the ability to translate the extracellular danger signal ATP into cytoprotective responses. For this purpose, P2Y11 engages cAMP, Ca2+ and PKC signaling to promote IL-1R dependent anti-inflammatory and pro-angiogenic responses in human M2 macrophages (Fig. 10). We previously demonstrated that P2Y11 anti-inflammatory signaling includes the release of soluble TNF receptors as well as the suppression of LPS-induced TNF-α secretion [15]. Pro-angiogenic effects of P2Y11 could also be detected but were limited to CXCL8 (IL-8) secretion [8, 10, 15].
To confirm and extend the concept of P2Y11/IL-1R anti-inflammatory and pro-angiogenic signaling, we have now performed the first NanoString-based transcriptome analysis of native P2Y11 activation in its natural environment as a starting point and then validated the regulation of promising candidate genes at the protein level. In accordance with our previous observation in the astrocytoma-based recombinant cell system [15], P2Y11 activation in primary human monocyte-derived macrophages resulted in strong upregulation of IL-1R mRNA and protein. Moreover, IL1RAP was also upregulated. IL1RAP, also known as IL-1R3, encodes the IL-1R accessory protein, which functions as a co-receptor of the ligand-binding IL1R1 in IL-1 signaling. This observation was surprising, because IL1RAP is normally not regulated [23], with the exception of certain myeloid malignancies, where upregulated IL1RAP is known to correlate with poor prognosis [42]. Given the enhanced expression of P2Y11 in myeloid cells [8, 14], upregulation of IL1RAP in these hematopoietic tumors may in fact be driven by P2Y11 signaling.
Although IL-1R was upregulated in response to P2Y11 activation, gene and cell surface expression of the ecto-ATPase CD39 were downregulated, revealing a useful mechanism that ensures stabilization of extracellular ATP, the natural P2Y11 agonist, and thus facilitates prolonged anti-inflammatory P2Y11 signaling. The anti-inflammatory signature of P2Y11 activation that emerged from the NanoString analysis was impressive and included downregulation of the pro-inflammatory, pro-apoptotic ATP receptor P2X7 as well as of TLR5, TLR7 and TLR8. Moreover, in line with the current view that GPCRs can modulate NLRP3 inflammasome activation [43], P2Y11 activation also caused downregulation of all three NLRP3 inflammasome components (NLRP3, CASP1, PYCARD).
An inevitable consequence of P2Y11-induced inflammasome deactivation in M2 macrophages is their inability to secrete bioactive IL-1ß. The concomitant P2Y11-driven upregulation of IL-1R, on the other hand, renders P2Y11-activated M2 macrophages highly sensitive to exogenous IL-1 cytokines, which cause IL-1R activation and MyD88-dependent signaling ultimately leading to the induction of TGFß-activated kinase 1 (TAK1), IkB kinase (IKK) or mitogen-activated protein kinases (MAPK) (ERK, JNK, p38) (Fig. 10) [23, 44]. In this manner, IL-1R can support P2Y11, which has previously been shown to engage IKK and ERK as critical signaling components [8]. P2Y11-driven reprogramming thus enables M2 macrophages to respond efficiently to IL-1 produced during the pro-inflammatory phase by initiating an anti-inflammatory process that finally facilitates the resolution of inflammation.
The anti-inflammatory and pro-angiogenic responses triggered by P2Y11 also depended on Ca2+ and cAMP. The crosstalk between the second messengers Ca2+ and cAMP is well documented [45]. Our observation that Ca2+ chelation using BAPTA-AM suppressed ATPγS-induced and rolipram-enhanced responses to a similar degree suggested that the rolipram-induced increase in intracellular cAMP may serve to support Ca2+ signaling. cAMP activates PKA, which further promotes Ca2+ release from internal stores by directly phosphorylating voltage-gated Ca2+ channels [45]. The resulting high concentrations of Ca2+ in turn stimulate Ca2+-dependent ACs. P2Y11, which is the only P2Y receptor that couples to Gq (Ca2+) and Gs (cAMP), is perfectly equipped to initiate such mutual enhancement of Ca2+ and cAMP signaling. The resulting sustained levels of cAMP trigger translocation of the PKA catalytic subunit to the nucleus, where it supports the expression of anti-inflammatory genes [17].
The expression of SOCS proteins is induced or enhanced by pro-inflammatory cytokines, including IL-1ß, as part of a classical negative-feedback loop. In our study, the expression of SOCS3, a major regulator of inflammation [46], was upregulated during P2Y11 signaling and strongly enhanced by cAMP. This is in accordance with a previous report that had identified SOCS3 as a target of the cAMP effector Epac1 [18], and with the finding that SOCS3 can antagonize the effects of cAMP [47]. Altogether, these observations suggested that an Epac1-SOCS3 axis regulates cAMP signaling induced by P2Y11. In line with this consideration, Epac1 inhibitor (R)-CE3F4 further enhanced P2Y11-driven and ADAM17-mediated sTNFR2 release resulting in even higher levels of sTNFR2. In addition, Epac1 inhibition further increased P2Y11/IL-1R-induced CCL20 secretion. Our results thus establish a cAMP surveillance function of Epac1 in P2Y11 signaling and represent an example of ADAM17 regulation by Epac1, which has not been reported before. Epac1 has previously been implicated in P2X7/P2Y11 signaling in tumor-derived vascular endothelium, because pharmacological Epac1 activation reproduced effects that were observed after P2 receptor activation [48]. However, this work did not clarify whether increased cAMP levels resulted from AC activation through P2Y11-activated Gs proteins or through P2X7-mobilized Ca2+. In addition, NF157 was used to imply P2Y11 involvement. In contrast to NF340, NF157 is not fully selective among P2 receptors and has limited potency at the P2Y11 receptor [10, 13].
The exact targets of the Epac1/SOCS3-mediated control of P2Y11 signaling have not yet been identified. However, SOCS3, which is also activated by IL-1, has been shown to inhibit IL-1 signaling as part of a negative-feedback regulation by targeting the TRAF6/TAK1 complex [25]. Specifically, SOCS3 inhibited the IL-1 induced IKK/NF-κB and JNK/p38 pathways by binding and inactivating TRAF6. In addition, cAMP has been shown to inhibit ERK via Epac1-dependent induction of SOCS3 [49], altogether suggesting that P2Y11/IL-1R anti-inflammatory signaling is itself limited by a cAMP/Epac1/SOCS3-axis, which dampens IKK/NF-κB and MAPK pathways (Fig. 8). Moreover, P2Y11-induced IL-1R2 (IL1R2) and IL-1R antagonist (IL1RN) may contribute to the self-limitation of P2Y11/IL-1R signaling.
In addition to the anti-inflammatory effects, P2Y11 signaling caused an angiogenic switch. We, and others, previously reported IL8 (CXCL8) as a target of P2Y11 signaling [8, 10, 15]. Here, we identified the pro-angiogenic factors VEGF [50] and CCL20 [51] as additional P2Y11 targets. Consistent with the P2Y11/IL-1R crosstalk described in the present study, both VEGF and CCL20 are known to be induced by IL-1 [52, 53]. Compared to VEGF, CCL20 secretion was more strictly controlled. Although CCL20 mRNA was strongly increased in response to P2Y11 stimulation and further enhanced in the presence of rolipram, CCL20 protein could not be detected. IL-1 cytokines were obviously required to induce CCL20 mRNA translation and CCL20 secretion. In line with the work from Dinarello’s lab [38, 54], P2Y11 may serve as the first signal that rapidly activates the genes encoding IL-1 and IL-1R as well as CCL20. Cells containing untranslated or poorly translated mRNAs are now primed and small amounts of a second signal may immediately trigger mRNA translation. IL-1 is known to act as a second signal and to enhance its own expression [38], explaining our current observation that recombinant IL-1 can enhance all P2Y11-induced secretory responses. However, in accordance with our kinetic analyses, the particularly strong responses that are induced by P2Y11/cAMP-mediated IL-1R activation and enhanced by exogenous IL-1 are likely to depend on prolonged signaling and de novo or enhanced gene expression.
Our NanoString-based mRNA expression analysis revealed that human monocyte-derived macrophages generated with M-CSF did not express CCR6, the sole receptor for CCL20, indicating that the P2Y11-induced release of CCL20 represents a form of paracrine signaling.
The CCL20/CCR6 axis has been shown to promote cancer progression through pro-angiogenic and immunosuppressive effects as well as through direct stimulation of cancer cell migration and proliferation [55]. CCL20 may be pro-angiogenic by inducing invasion, sprouting and migration of CCR6-expressing endothelial cells [51]. CCL20 produced in the tumor microenvironment may also selectively attract Th17 cells expressing high levels of CCR6 [56]. This CCR6+ Th subset has been implicated in the IL-17-mediated induction of pro-angiogenic factors in the tumor microenvironment [57, 58]. Finally, the CCL20/CCR6 axis may also be immunosuppressive by inducing the selective recruitment of FoxP3-positive regulatory T cells [59]. Taken together, CCL20 secreted from P2Y11-activated M2 macrophages may directly promote tumor growth or indirectly support tumor progression through the recruitment of regulatory and pro-angiogenic immune cells.
CCL20 has also been implicated in inflammatory processes including inflammatory diseases [55], raising the question of whether P2Y11/IL-1R-driven CCL20 production is pro- or anti-inflammatory. Although we have no clear answer yet, it is important to note that ATPγS in combination with IL-1 induced only low levels of CCL20. An increase in intracellular cAMP, which is mainly considered to be anti-inflammatory [17], was required to enable robust CCL20 secretion, suggesting that P2Y11/IL-1R-induced CCL20 serves homeostatic purposes.
In summary, our work in human M2 macrophages provides strong evidence that the G protein-coupled ATP receptor P2Y11 develops its cyto- and tissue-protective effects through crosstalk with IL-1R (Fig. 10). P2Y11 engages all components of canonical signaling (Ca2+, PKC and cAMP) to stimulate anti-inflammatory and pro-angiogenic responses. P2Y11 has great potential for therapeutic development. From a translational point of view, targeting of P2Y11 may be desirable in inflammatory and infectious diseases as well as in malignant disorders. Although agonistic P2Y11 targeting would be required to attenuate macrophage-mediated hyper-inflammation as for instance associated with Covid-19 [60], antagonistic targeting would be necessary for an effective therapy of cancer. Myeloid malignancies with elevated IL1RAP [42] and CCR6+ cancers that respond to CCL20 such as liver cancer [55] might be first candidates.
Data availability
All data generated or analyzed during this study are included in this published article. Additional transcriptome profiling data are also available on request from the corresponding author [MT]. Some data may not be made available because of privacy or ethical restrictions.
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Open access funding provided by Austrian Science Fund (FWF). This work was supported by the Austrian Science Fund (P 33640-B to MT).
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DK and MT designed and supervised the study. HG, GD and DK performed monocyte isolation, differentiation and stimulation as well as propagation of cell lines. AR carried out cytokine measurements. DK and HG operated the Canto II flow cytometer. DK and MT analyzed and interpreted data. DK and MT prepared graphs, wrote and revised the manuscript. All authors reviewed, revised, and approved the manuscript for publication.
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Klaver, D., Gander, H., Dobler, G. et al. The P2Y11 receptor of human M2 macrophages activates canonical and IL-1 receptor signaling to translate the extracellular danger signal ATP into anti-inflammatory and pro-angiogenic responses. Cell. Mol. Life Sci. 79, 519 (2022). https://doi.org/10.1007/s00018-022-04548-z
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DOI: https://doi.org/10.1007/s00018-022-04548-z