Summary
Four well differentiated gastric adenocarcinoma cell lines from German patients have been established from primary tumors (St 23132, St 3051) and lymph node metastases (St 2474, St 2957). The tumor cells were isolated by enzymatic or mechanical treatment. All four lines grew as solid tumors in nude mice and formed colonies in soft agar. The doubling time of the cells in culture was 25–32 h. Further characteristics of the lines were a considerable chromosomal aneuploidy, (the chromosomal numbers varying from 30–109 with many numerical and structural abnormalities), a stable keratin expression (Ck 8, 18, 19), the expression and secretion of CEA and CA-19-9 and the overexpression of c-myc. The four stomach cancer cell lines described here are not only a useful addition to the small number of existing lines, but also represent ideal tools for studying tumorigenicity of human stomach cancers in vitro and in vivo.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- FCS :
-
fetal calf serum
- PBS :
-
posphate-buffered saline
- SDS :
-
sodium dodecyl sulfate
References
Akiyama S, Amo H, Watanabe T, Matsuyama M, Sakamato J, Imaizumi H, Kondo T, Takagi H (1988) Characteristics of three human gastric cancer cell lines, NU-GC-2, NU-GC-3 and NU-GC-4. Jpn J of Surg 18:438–446
Bos JL, Veerian-de Fries M, Marshall CJ, Veeneman GH, Van Boom JH, Vander Eb AJ (1986) A human gastric carcinoma contains a single mutated and an amplified normal allele of the Ki-ras oncogene. Nucl Acids Res 12:1209–1217
Comings DE, Peters KE (1981) Two-dimensional gel electrophoresis of nuclear particles. Cell Nucleus 9:89–118
Correa P (1985) Clinical implications of recent developments in gastric cancer pathology and epidemiology. Semin Oncol 12:2–10 (1985)
Dippold WG, Klingel R, Kerlin M, Schwaeble M, Meyer zum Büschenfelde KH (1991) Stimulation of pancreas and and gastric cell growth by interleukin 3 and granulocyte-macrophage colony-stimulating factor. Gastroenterology 100:1338–1344
Erisman MD, Rothberg PG, Diehl RE, Morse CC, Spandorfer JM, Astrin SM (1985) Deregulation of c-myc gene expression in human colon carcinoma is not accompanied by amplification or rearrangements of the gene. Mol Cell Biol 5:1969–1976
Faller G, Vollmers HP, Weiglein I, Pfaff M, Zink C, Marx A, Müller-Hermelink HK (1990) HAB-1, a new heteromyeloma for stable human monoclonal antibody-production. Br J Cancer 62:595–598
Ferti-Passantanopoulou AD, Panani AD, Vlachos JD, Raptis SA (1987) Common cytogenetic findings in gastric cancer. Cancer Genet Cytogenet 24:63–73
Ferti-Passantanopoulou AD, Panani AD, Raptis S (1988) Cytogenetic study of rectosigmoidal adenocarcinomas. Cancer Genet Cytogenet 34:101–104
Gown AM, Vogel AM (1982) Monoclonal antibodies to intermediate filament protein of human cells: unique and cross-reacting antibodies. J Cell Biol 95:414–424
Hancock K, Tsang VCW (1982) India ink staining of proteins on nitrocellulose paper. Anal Biochem 133:157–162
Hojo J (1977) Establishment of cultured cell lines of human stomach cancer origin and their morphological characteristics. Niigata Igakusai Zassi 91:737–763
Laboisse CL, Augeron C, Couturier-Turpin MH, Gespach C, Cheret AM, Potet F (1982) Characterization of a new human gastric cancer cell line HGT-1 bearing histamin H2-receptor. Cancer Res 42:1541–1548
Laemmli UK (1978) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lin CH, Fu ZM, Liu YI, Jang JL, Xu JF, Chen OS, Chen HM (1984) Investigation of SGC-7901 cell line established from human gastric carcinoma cells. Chin Med J 97:831–834
Little CD, Nau MM, Carney DN, Gazdar AF, Minna JD (1983) Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306:194–196
MacDonald JS, Cohn I, Gunderson LL (1985) Cancer of the stomach. In: DeVita VT, Hellmann S, Rosenberg SA (eds). Cancer, principles and practice of oncology. JB Lippincott Co, Philadelphia, pp 659–690
Mettlin C (1988) Epidemiologic studies in gastric adenocarcinoma. In: Douglass HO (ed). Gastric cancer. Churchill Livingstone, New York, pp 1–25
Moll R, Franke WW, Schiller DL (1982) The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24
Motoyama T, Hojo H, Watanabe H (1986) Comparison of seven cell lines from human gastric carcinomas. Acta Pathol Jpn. 36:65–83
Müller J, O’Connoer R, Stulle K, Vollmers HP, Müller-Hermelink HK (1988) Charakterisierung von Zellinien menschlicher Magenkarzinome. Verh Dtsch Ges Pathol 72:214–217
Nakasato F, Sakamoto H, Mori M, Hayashi K, Shimosato Y, Nishi M, Takao S, Nakatani K, Terada M, Sugimura T (1984) Amplification of the c-myc oncogene in human stomach cancers. Gann 75:737–742
O’Farrell PH (1975) High-resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021
O’Hara B, Oskarsson M, Tainsky M, Blair DG (1986) Mechanism of activation of human ras genes cloned from a gastric adenocarcinoma and a pancreatic carcinoma cell line. Cancer Res 46:4695–4700
Ohuchi N, Horan Hand P, Merlo G, Fujita J, Mariani-Constantini R, Thor A, Nose M, Callahan R, Schlom J (1987) Enhanced expression of c-Ha-ras p21 in human stomach adenocarcinomas defined by immunoassays using monoclonal antibodies and insitu hybridisation. Cancer Res 47:1413–1420
Park JG, Frucht H, LaRocca RV, Bliss DP, Kurita Y, Chen TR, Henslee JG, Trepel JB, Jensen RT, Johnson BE, Bang YJ, Kim JP, Gazdar AF (1990) Characteristics of cell lines established from human gastric carcinoma. Cancer Res 50:2773–2780
Pfaff M, O’Connor R, Vollmers HP, Müller-Hermelink HK (1990) Human monoclonal antibody against a tissue polypeptide antigen-related protein from a patient with a signet-ring cell carcinoma of the stomach. Cancer Res 50:5192–5198
Saal K, Vollmers HP, Höhn H, Müller-Hermelink HK (1988) Cytogenetic studies on human gastric cancers. Cancer Genet Cytogenet 38:74
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press
Shibuya M, Yokota J, Ueyama Y (1985) Amplification and expression of a cellular oncogene (c-myc) in human gastric adenocarcinoma cells. Mol Cell Biol 5:414–418
Tahara E (1990) Growth factor receptors and oncogene in human gastrointestinal carcinomas. Cancer Res Clin Oncol 116:121–131
Terano A, Nakada R, Mutoh H, Hiraishi H, Ota S, Shiina S, Shimada T, Itoh Y, Kimura K, Shiga J (1991) Characterization of a newly established cell line (JR-ST) derived from human gastric gastric signet ring cell cancer, producing tumor markers. Gastroenterol Jpn 26:7–13
Vollmers HP, Birchmeier W (1983) Monoclonal antibodies inhibit the adhesion of B16 melanoma cells in vitro and block lung metastases in vivo. Proc Natl Acad Sci USA 80:6863–6869
Vollmers HP, Birchmeier W (1983) Monoclonal antibodies that prevent adhesion of B16 melanoma cells and reduce metastases in mice: crossreaction with human cells. Proc Natl Acad Sci USA 80:3729–3733
Vollmers HP, Imhof B, Wieland I, Birchmeier W (1985) Monoclonal antibodies NORM-1 and NORM-2 induce more normal behaviour in tumor cells in vitro and in vivo. Cell 40:547–557
Vollmers HP, O’Connor R, Müller J, Kirchner T, Müller-Hennelink HK (1989) SC-1, a functional human monoclonal antibody against autologous stomach carcinoma cells. Cancer Res 49:2471–2476
Weinstock J, Baldwin GS (1988) Binding of gastrin 17 to human gastric carcinoma cell lines. Cancer Res 48:932–937
Yamamoto T, Ikawa S, Akivagma T, Semba K, Nomura N, Mivajima N, Saito T, Tovoshima K (1986) Similarity of protein encoded by the human c-erbB-2 gene to epidermal growth factor receptor. Nature 319:230–234
Yanagihara K, Sevama T, Tsumurava M, Kamada N, Yokoro K (1991) Establishment and characterization of human signet ring cell gastric carcinoma cell lines with amplification of the c-myc oncogene. Cancer Res 1:381–386
Yoshida K, Takanasha A, Kvo E, Ito M, Niimoto M, Hattori T, Tahara E (1989) Epidermal growth factor induces the expression of its receptor gene in human gastric carcinoma cell line TMK-1. Jpn J Cancer Res 80:743–746
Yoshikawa K, Ueda R, Obata Y, Utsumi KR, Notake K, Takashi T (1986) Human monoclonal antibody reactive to stomach carcinoma produced by mouse-human hybridoma technique. Jpn J Cancer Res 77:1122–1133
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vollmers, H.P., Stulle, K., Dämmrich, J. et al. Characterization of four new gastric cancer cell lines. Virchows Archiv B Cell Pathol 63, 335–343 (1993). https://doi.org/10.1007/BF02899281
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02899281