Abstract
A protonemal branch was induced on a side wall of a fern filamentous protonema by cell centrifugation and subsequent polarized-red light irradiation as described in a previous paper (Wada 1995, J. Plant Res. 108: 501–509). Changes in microtubule (MT) and microfilament (MF) patters during the branch development were observed under fluorescence microscopy. A ring-like band of cortical MTs (MT-ring) and MFs similar to a preprophase band or a subapical ring structure (Murataet al. 1987) appeared transiently at the future branching site before cell swelling, the first visible step of branch formation. At this stage, the nucleus was located far from the branching site and the MT-ring appeared to be connected to the nucleus by endoplasmic MFs as well as with endoplasmic MTs. The MT-ring disappeared when cell wall swelling occurred. When the cell wall swelling began, a fan-like pattern of cortical MTs emanating from the new growing tip was established and the MTs reached the opposite flank of the protonema. When a new branch started to elongate and the nucleus moved into the branch, a faint subapical ring of MTs appeared at the subapical part of the new branch. Strands of MTs and MFs emanating from the nuclear front end reached a part of the subapical ring.
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Abbreviations
- MT:
-
microtubule
- MF:
-
microfilament
- DAPI:
-
4′, 6, diamidino-2-phenylindole
References
Doonan, J. H. 1991. The cytoskeleton and moss morphogenesis.In C.W. Lloyd, ed., The Cytoskeletal Bases of Plant Growth and Form. Academic Press, London, pp. 289–301.
Doonan, J.H., Jenkins, G.I., Cove, D.J. andLloyd, C.W. 1986. Microtubule connect the migrating nucleus to the prospective division site during side branch formation in the moss,Physcomitrella patens. Eur. J. Cell Biol.41: 157–164.
Joos, U., van Aken, J. andKristen, U. 1994. Microtubules are involved in maintaining the cellular polarity in pollen tubes ofNicotiana sylvestris. Protoplasma179: 5–15.
Kadota, A. andWada, M. 1989. Circular arrangment of cortical F-actin around the subapical region of a tipgrowing fern protonemal cell. Plant Cell Physiol.30: 1183–1186.
Kadota, A. andWada, M. 1995. Cytoskeletal aspects of nuclear migration during tip-growth in the fernAdiantum protonemal cell. Protoplasma188: 170–179.
Kiss, J.Z., Wang-Cahill, F. andKiss, H.G. 1995. The ultrastructure of the early development ofSchizaea pusilla gametophytes. Inter. J. Plant. Sci.156: 131–142.
Lancelle, S.A., Cresti, M. andHepler, P.K. 1987. Ultrastructure of the cytoskelenton in freeze-substituted pollen tubes ofNicotiana alata. Protoplasma140: 141–150.
Lloyd, C.W. 1983. Helical microtubular arrays in onion root hairs. Nature305: 311–313.
Lloyd, C.W., Pearce, K.J., Raulins, D.J., Ridge, R.W. andShaw, P.J. 1987. Endoplasmic microtubules connect the advancing nucleus to the tip of legume root hairs, but F-actin is involved in basipetal migration. Cell Motil. Cytoskel.8: 17–36.
Mineyuki, Y. andFuruya, M. 1986. Involvement of colchicine-sensitive cytoplasmic element in premitotic nuclear positioning ofAdiantum protonemata. Protoplasma130: 83–90.
Murata, T., Kadota, A., Hogetsu, T. andWada, M. 1987. Circular arrangement of cortical microtubules around the subapical part of a tip-growing fern protonema. Protoplasma141: 135–138.
Murata, T. andWada, M. 1989a. Re-organization of microtubules during preprophase band development inAdiantum protonemata. Protoplasma151: 73–80.
Murata, T. andWada, M. (1989b. Organization of cortical microtubules and microfibril deposition in reponse to blue-light induced apical swelling in a tip-growingAdiantum protonemal cell. Planta178: 334–341.
Murata, T. andWada, M. 1992. Cell cycle specific disruption of the preprophase band of microtubules in fern protonemata: Effects of displacement of the endoplasm by centrifugation. J. Cell Sci.101: 93–98.
Ootaki, T. 1965. Branching in fern gametophyte,Pteris vittata, under various light conditions. Cytologia30: 182–193.
Picket-Heaps, J.D. andNorthcote, D.H. 1966a. Organization of microtubules and endoplasmic reticulum during mitosis and cytokinesis in wheat meristems. J. Cell. Sci.1: 109–120.
Picket-Heaps, J.D. andNorthcote, D.H. 1966b. Cell division in the formation of the stomatal complex of the young leaves of wheat. J. Cell. Sci.1: 121–128.
Schmiedel, G. andSchnepf, E. 1979. Side branch formation and orientation in the caulonema of the moss,Funaria hygrometrica: Normal development and fine structure. Protoplasma100: 367–383.
Schmiedel, G. andSchnepf, E. 1980. Polarity and growth of caulonema tip cells of the mossFunaria hygrometrica. Planta147: 405–413.
Shibaoka, H. 1994. Plant hormone-induced changes in the orientation of cortical microtubules: Alterations in the cross linking between microtubules and the plasma membrane. Annu. Rev. Plant Physiol. Plant Mol. Biol.45: 527–544.
Stetler, D.A. andDeMaggio, A.E. 1972. An ultrastructural study of fern gametophytes during one-to two-dimensional development. Amer. J. Bot.59: 1011–1017.
Terasaka, O. andNiitsu, T. 1994. Differential roles of microtubule and actin-myosin cytoskeleton in the growth ofPinus pollen tubes. Sex Plant Reprod.7: 264–272.
Vogelmann, T. C., Bassel, A.R. andMiller, J.H. 1981. Effects of microtubule inhibitors on nuclear migration and rhizoid differentiation in germinating fern spores (Onoclea sensibilis). Protoplasma109: 295–316.
Wada, M. 1995. Nuclear behavior during branch formation in a centrifugedAdiantum protonema and the nuclear polarity. J. Plant Reser.108: 501–509.
Wada, M. andMurata, T. 1991. The cytoskeletons in fern protonemal growth in relation to photomorphogenesis.In C.W. Lloyd, ed., The Cytoskeletal Basis of Plant Growth and Form. Academic Press, London, pp. 277–288.
Wada, M. andO'Brien, T.P. 1975. Observations on the structure of the protonema ofAdiantum capillus-veneris L. undergoing cell division following white-light irradiation. Planta126: 213–237.
Wada, M. andSugai, M. 1994. Photobiology of ferns.In R.E. Kendrick and G.H.M. Kronenberg, eds., Photomorphogenesis in Plants. Kluwer Academic Publishers, Dordrecht. pp. 783–802.
Webb, M.C. andGunning, B.E.S. 1991. The microtubular cytoskeleton during development of the zygote, proembryo and free-nuclear endosperm inArabidopsis thaliana (L.) Heynh. Planta184: 187–195.
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Wada, M., Nozue, K. & Kadota, A. Cytoskeletal pattern changes during branch formation in a centrifugedAdiantum protonema. J. Plant Res. 111, 53–58 (1998). https://doi.org/10.1007/BF02507149
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DOI: https://doi.org/10.1007/BF02507149