Abstract
The proliferative activity of serrated adenomas of the large intestine was determined by examining proliferating cell nuclear antigen (PCNA). The PCNA labeling index, determined by visual inspection, and the PCNA area rate, determined with the newly developed image processor for analytical pathology (IPAP), of serrated adenoma were found to be similar to the values for tubular adenoma, and indicated the presence of high proliferative activity in the bottoms of crypts. Determination of the pattern of distribution of PCNA-positive cells indicated the presence of a proliferative zone in the lower region or bottom of the serrated adenoma. However, 5 of the 20 serrated adenomas exhibited an irregular on widely extended proliferative zone, and 2 were complicated by cancer. These findings indicated that serrated adenoma is also a highly proliferative tumor and that it may be complicated by cancer if atypia is increased and disturbance of the proliferative zone is present.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Longacre TA, Fenoglio-Preiser CM. Mixed hyperplastic adenomatous polyps/serrated adenoma. Am J Surg Pathol 1990;14:524–537.
Urbanski SJ, Macron N, Kossakowska AE, et al. Mixed hyperplastic adenomatous polyps: An underdiagnosed entity. Am J Surg Pathol 1984;8:551–556.
Fenoglio-Preiser CW. Hyperplastic polyps, adenomatous polyps, and mixed hyperplastic adenomatous polyps of the colon: Definitions. In: Karr JP (ed) Basic clinical perspectives of colorectal polyps and cancer. New York: Alan R. Liss, 1988;3–12.
Wada R, Abe H, Miwa H, et al. Examination of cell dynamics in various epithelial lesions in the large intestine by in vitro BrdU labeling (in Japanese). Cancer Clin 1991;37:119–123.
Miyachi K, Fritzler MJ, Tan EM. Autoantibody to a nuclear antigen in proliferating cells. J Immunol 1978;121:2228–2234.
Takasaki Y, Deng JS, Tan EM. A nuclear antigen associated with cell proliferation and blast formation. J Exp Med 1981;154:1899–1909.
Bravo R, Fey SJ, Bellatin J, et al. Identification of a nuclear antiger and a cytoplasmic polypeptide whose relative proportions are sensitive to change in the rate of cell proliferation. Exp Cell Res 1981;136:311–319.
Lee MYWT, Tan CK, Downey KM, et al. Further studies of calf thymus DNA polymerase delta purified to homogeneity by a new procedure. Biochemistry 1984;23:1906–1913.
Bravo R, Frank R, Brundell PA, et al. Cyclin/PCNA is the auxiliary protein of DNA polymerase-δ. Nature 1987;326:515–517.
Bravo R, Macdonald-Bravo H. Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle. J Cell Biol 1987;168:1549–1554.
Ogata K, Kurki P, Cellis JE, et al. Monoclonal antibodies to a nuclear protein (PCNA/cyclin) associated with DNA replication sites. Exp Cell Res 1987;168:475–486.
Morris GF, Mathews MB. Regulation of proliferating cell nuclear antigen during the cell cycle. J Biol Chem 1989;264: 13856–13864.
Matsuno Y, Hirohashi S, Fukuya S, et al. Heterogeneity of proliferative activity in nodule-in-nodule lesions of small hepatocellular carcinoma. Jpn J Cancer Res 1990;81:1137–1140.
Theunissen PHMH, Leers MPG, Bollen ECM. Proliferating cell nuclear antigen antigen (PCNA) expression in formalinfixed tissue of non-small cell lung carcinoma. Histopathology 1993;20:251–255.
Sitonen SM, Kallioniemi OP, Isola JJ. Proliferating cell nuclear antigen immunohistochemistry using monoclonal antibody 19A2 and a new antigen retrieval technique has prognostic impact in archival paraffin-embedded node-negative breast cancer. Am J Pathol 1993;142:1081–1089.
Hall PA, Levision DA, Woods AL, et al. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: An index of cell proliferation with evidence of deregulated expression in some neoplasmas. J Pathol 1990;162:285–294.
Robbins BA, Vega D, Ogata K, et al. Immunohistochemical detection of proliferating cell nuclear antigen in solid human malignancies. Arch Pathol Lab Med 1987;111:841–845.
Kawakita N, Seki S, Sakaguchi H. Analysis of proliferating hepatocytes using a monoclonal antibody aggainst proliferating cell nuclear antigen/cyclin in embedded tissues from various liver diseases fixed in formaldehyde. Am J Pathol 1992;140:513–520.
Al-Sheneber IF, Shibata HR, Sampalis J, et al. Prognostic significance of proliferating cell nuclear antigen expression in colorectal cancer. Cancer 1992;71:1954–1959.
Jain S, Filipe PA, Hall PA, et al. Prognostic value of proliferating cell antigen in gastric carcinoma. J Clin Pathol 1991;44:655–659.
Watanabe T, Katsura Y, Yoshitake A. IPAP: Image processor for analytical pathology. J Toxicol Pathol 1994;7:353–361.
Haapasalo HK, Sallinen PK, Hellen PT, et al. Comparison of three quantification methods for PCNA immunostaining: Applicability and relation to survival in 83 astrocytic neoplasmas. J Pathol 1993;171:207–214.
Goldman H, Ming S-C, Hickok DF. Nature and significance of hyperplastic polyps of the human colon. Arch Pathol 1970;89:349–354.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fujishima, N. Proliferative activity of mixed hyperplastic adenomatous polyp/serrated adenoma in the large intestine, measured by PCNA (proliferating cell nuclear antigen). J Gastroenterol 31, 207–213 (1996). https://doi.org/10.1007/BF02389519
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02389519