Summary
General conditions for continuous expression of heterologous genes fromP L promoter in two fermenters connected in series have been established. The induction time of the bacterial cells is calculated as a function of the retention time in the inducing reactor. Using this model, it is possible to adapt fermentation parameters to the particular behaviour of any specific recombinant clone.
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Abbreviations
- F(t) :
-
flow at timet [ml/min]
- M T (t) :
-
culture induced, at timeT of fermentation, during a period up tot [ml]
- N T (t) :
-
culture induced, at timeT of fermentation, during a period fromt tot+dt [ml]
- p(t) :
-
product yield in a discontinuous culture [units/ml]
- P(t) :
-
product yield at the outlet of the fermenter [units/ml]
- v(t) :
-
volume of culture entered into the inducing reactor up to timet [ml]
- V :
-
volume of the inducing reactor [ml]
- θ:
-
retention time in the inducing reactor [min]
- τ(t):
-
average induction time at timet [min]
References
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Cubarsí, R., Benito, A., Vidal, M. et al. A model for continuous production of thermally induced recombinant proteins. Biotechnol Lett 13, 249–254 (1991). https://doi.org/10.1007/BF01041479
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DOI: https://doi.org/10.1007/BF01041479