Summary
The presence in human gastric juice of a lipase secreted by the gastric mucosae has been reported previously, but its exact cellular origin has not yet been established. Polyclonal antibodies specific to human gastric lipase (HGL) were prepared, and used by an immunofluorescence technique to label cells producing HGL. This immunocytolocalization was correlated with that of pepsin (chief cells) and parietal cells using specific polyclonal or monoclonal antibodies.
Our results clearly establish that HGL is exclusively located in the chief cells of fundic mucosa; furthermore, it was found to be always co-located with pepsin. No HGL was observed in the parietal or mucus cells. HGL was always detected intracellularly, either in secretory granules of the apical region of the chief cells, or revealed by more diffuse cytoplasmic labelling.
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Abbreviations
- HGL:
-
Human gastric lipase
- SDS PAGE:
-
Sodium dodecyl sulfate-polyAcrylamid gel electrophoresis
- PBS:
-
Phosphate buffer saline
References
Abrams CK, Hamosh M, Lee TC, Ansher AF, Collen MJ, Benjamin SB, Hamosh P (1986) Gastric lipase in the human stomach. Clin Res 34:436A
Barrowman JA, Darnton SJ (1970) The lipase of rat gastric mucosa. A histochemical demonstration of the enzymatic activity against a medium chain triglyceride. Gastroenterology 59:13–21
Bodmer MW, Angal S, Yarranton GT, Harris TJR, Lyons A, King DJ, Pieroni G, Rivière C, Verger R, Lowe PA (1987) Molecular cloning of a human gastric lipase and expression of the active enzyme in yeast. Biochim Biophys Acta 909:237–244
De Nigris SJ, Fink CS, Hamosh M, Kasbekar DK, Hamosh P (1985) Fat digestion: species differences in the source of preduodenal lipolytic enzymes. Fed Proc 44:811
De Nigris SJ, Hamosh M, Kasbekar DK, Fink CS, Lee TC, Hamosh P (1985) Secretion of human gastric lipase from dispersed gastric glands. Biochim Biophys Acta 836:67–72
Feracci H, Maroux S (1980) Rabbit intestinal aminopeptidase N. Purification and molecular properties. Biochim Biophys Acta 599:448–468
Gargouri Y, Pioroni G, Riviére C, Saunière JF, Lowe PA, Sarda L, Verger R (1986) Kinetic assay of human gastric lipase on short and long chain triacylglycerol emulsions. Gastroenterology 91:919–925
Hamosh M (1984) Lingual lipase. In: Borgström B, Brockman HL (eds) Lipases. Elsevier, Amsterdam, pp 49–81
Hamosh M (1986) Lingual lipase. Gastroenterology 90:1290–1292
Moreau H, Gargouri Y, Lecat D, Junien JL, Verger R (1988a) Screening of preduodenal lipases in several mammals. Biochim Biophys Acta 959:247–252
Moreau H, Gargouri Y, Bernadac A, Pieroni G, Laugier R, Saunière JF, Lecat D, Junien JL, Verger R (1988b) Etude biochimique et physiologique des lipases préduodénales d'origines animale et humaine: revue. Rev Fr Corps Gras 4:169–176
Moreau H, Laugier R, Gargouri Y, Verger R (1988c) Human gastric lipase is entirely of gastric fundic origin. Gastroenterology 95:1221–1226
Moreau J, Stupnick S, Bouisson M, Bertrand C, Balas D, Ribet A (1987) Localisation et aspects quantitatifs de la sécrétion de la lipase gastrique chez le sujet sain et l'insuffisant pancréatique exocrine (IPE). Gastroentérol Clin Biol 11:213A
Perret JP (1982) Lipolyse gastrique chez le lapereau. Origine et importance physiologique de la lipase. J Physiol (Paris) 78:221–230
Roberts IM, Jaffe R (1986) Lingual lipase: immunocytochemical localization in the rat Von Ebner gland. Gastroenterology 90:1170–1175
Schonheyder F, Volquartz K (1946) The gastric lipase in man. Acta Physiol Scand 11:349–360
Szafran Z, Szafran H, Popiela T, Trompeter G (1978) Coupled secretion of gastric lipase and pepsin in man following pentagastrin stimulation. Digestion 18:310–318
Tiruppathi C, Balasubramanian KA (1982) Purification and properties of an acid lipase from human gastric juice. Biochim Biophys Acta 712:692–697
Tokuyasu KT (1973) A technique for ultracryotomy of cell suspension and tissues. J Cell Biol 503–509
Towbin H, Stachelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:503–509
Vatier H (1983) Pepsinogène, pepsine: sécrétion, rôle physiologique, approche biochimique. Gastroentérol Quotid 1:19–26
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Moreau, H., Bernadac, A., Gargouri, Y. et al. Immunocytolocalization of human gastric lipase in chief cells of the fundic mucosa. Histochemistry 91, 419–423 (1989). https://doi.org/10.1007/BF00493829
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DOI: https://doi.org/10.1007/BF00493829