Summary
The genetic relationships between two Prunus species, involved in rootstock breeding, were examined at the level of the ribosomal RNA genes. Twenty clones of P. cerasifera, a diploid species, and 12 clones of P. spinosa, a tetraploid wild species, were studied. The use of three heterologous ribosomal DNA probes covering different regions of the ribosomal tandem repeats enabled us to construct restriction maps for EcoRI and BamHI. We identified two unit types (unit I and unit II) in P. cerasifera. In P. spinosa, P. cerasifera units were present in addition to a third ribosomal unit type (unit III). These results appeared to confirm previous cytological studies (Salesses 1973) indicating that one of the genomes in P.spinosa has homology with the one from P. cerasifera.
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References
Appels R, Dvorak J (1982a) Theor Appl Genet 63:337–348
Appels R, Dvorak J (1982b) Theor Appl Genet 63:361–365
Appels R, Moran LB, Gustafson JP (1986) Can J Genet Cytol 28:673–685
Choumane W, Heizmann P (1988) Theor Appl Genet 76:481–489
Delcasso-Tremousaygue D (1987) Thèse de Doctorat de l'Université des Sciences et Techniques du Languedoc Montpellier II 164 p
Delseny M, Cooke R, Penon P (1983) Plant Sci Lett 30:107–119
Delseny M, McGrath JM, This P, Chevre AM, Quiros CF (1990) Genome 33:733–744
Doyle JJ, Doyle JL (1990) Focus 12 (1)
Eremin GV (1990) Acta Horticulturae 283:27–30
Eremin GV, Rassvetaeva EG, Kovaleva VV (1985) Trudy poprikladnoj botanike genetike i selektsii 77:39–42
Goldsbrough PB, Cullis CA (1981) Nucl Acid Res 9(6):301–1309
Grellet F, Delcasso-Tremousaygue D, Delseny M (1989) Plant Mol Biol 12:695–706
Kaneko T, Terachi T, Tsunewaki K (1986) Jpn J Genet 61:157–168
Keil RL, Roeder GS (1984) Cell 39:377–386
May CE, Appels R (1987) Theor Appl Genet 74:617–624
Nelson FK, Frankel W, Rajan TV (1989) Mol Cell Biol 9 (3):284–1288
Olmedilla A, Delcasso D, Delseny M, Cauwet-Marc AM (1985) Pl Syst Evol 150:263–274
Petes TD, Botstein D (1977) Proc Natl Acad Sci 74 (11):5091–5095
Russell PJ, Petersen RC, Wagner S (1988) Mol Gen Genet 211:541–544
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Proc Natl Acad Sci USA 81:8014–8018
Salesses G (1970) Arm Amélior Plantes 20:469–483
Salesses G (1973) Ann Amélior Plantes 23:145–161
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning A laboratory manual Cold Spring Harbor Laboratory Press
Seperack P, Slatkin M, Arnheim N (1988) Genetics 119:943–949
Vedel F, Delseny M (1987) Plant Physiol Biochem 25 (2):191–210
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Reynders-Aloisi, S., Grellet, F. Characterization of the ribosomal DNA units in two related Prunus species (P. Cerasifera and P. Spinosa). Plant Cell Reports 13, 641–646 (1994). https://doi.org/10.1007/BF00232937
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DOI: https://doi.org/10.1007/BF00232937