Abstract
The phylogenetic relationships among seven Linguatula serrata (L. serrata) isolates collected from cattle, goats, sheep, dogs and camels in different geographical locations of Iran were investigated using partial 18S ribosomal RNA (rRNA) and partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequences. The nucleotide sequences were analysed in order to determine the phylogenetic relationships between the isolates. Higher sequence diversity and intraspecies variation was observed in the cox1 gene compared to 18S rRNA sequences. Phylogenetic analysis of the cox1 gene placed all L. serrata isolates in a sister clade to L. arctica. The Mantel regression analysis revealed no association between genetic variations and host species or geographical location, perhaps due to the small sample size. However, genetic variations between L. serrata isolates in Iran and those isolated in other parts of the world may exist and could reveal possible evolutionary relationships.
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Alborzi A., Molayan P.H., Akbari M. 2013. Prevalence of Linguatula serrata Nymphs in Mesenteric Lymph Nodes of Cattle and Buffaloes Slaughtered in Ahvaz Abattoir, Iran. Iranian Journal of Parasitology, 8, 327–332
Beaver P.C. 1984. Clinical parasitology. Philadelphia: Lea & Febiger
Drabick J.J. 1987. Pentastomiasis. Review of Infectious Diseases, 9, 1087–1094
el-Hassan A.M., Eltoum I.A., el-Asha B.M. 1991. The Marrara syndrome: isolation of Linguatula serrata nymphs from a patient and the viscera of goats. Transactions of the Royal Society of Tropical Medicine and Hygiene, 85, 309
Fard S.R., Ghalekhani N., Kheirandish R., Fathi S., Asl E.N. 2012. The prevalence of Linguatula serrata nymphs in camels slaughtered in Mashhad slaughterhouse, Northeast, Iran. Asian Pacific Journal of Tropical Biomedicine, 2, 885–888. DOI:10.1016/S2221-1691(12)60247-0
Gjerde B. 2013. Phylogenetic position of Linguatula arctica and Linguatula serrata (Pentastomida) as inferred from the nuclear 18S rRNA gene and the mitochondrial cytochrome c oxidase subunit I gene. Parasitology Research, 112, 3517–3525. DOI:10.1007/s00436-013-3534-9
Gul A., Deger S., Denizhan V. 2009. The prevalence of Linguatula serrata (Frohlich, 1789) nymphs in sheep in the Van province. Turkiye parazitolojii dergisi / Turkiye Parazitoloji Dernegi — Acta Parasitologica Turcica/Turkish Society for Parasitology, 33, 25–27
Hendrix C.M. 1998. Diagnostic veterinary parasitology. St. Louis, MO: Mosby
Khalil G.M. 1976. Prevalence of Linguatula serrata infection in animals from the Cairo abattoir. The Journal of Parasitology, 62, 126
Khalil G.M., Schacher J.F. 1965. Linguatula serrata in relation to halzoun and the marrara syndrome. The American Journal of Tropical Medicine and Hygiene, 14, 736–746
Koehsler M., Walochnik J., Georgopoulos M., Pruente C., Boeckeler W., Auer H., Barisani-Asenbauer T. 2011. Linguatula serrata tongue worm in human eye, Austria. Emerging Infectious Diseases, 17, 870–872. DOI:10.3201/eid1705.100790
Maleky F. 2001. A case report of Linguatula serrata in human throat from Tehran, central Iran. Indian Journal of Medical Sciences, 55, 439–441
Meshgi B., Asgarian O. 2003. Prevalence of Linguatula serrata infestation in stray dogs of Shahrekord, Iran. Journal of Veterinary Medicine. B, Infectious diseases and veterinary public health, 50, 466–467
Oluwasina O.S., Thankgod O.E., Augustine O.O., Gimba F.I. 2014. Linguatula serrata (Porocephalida: Linguatulidae) Infection among Client-Owned Dogs in Jalingo, North Eastern Nigeria: Prevalence and Public Health Implications. Journal of Parasitology Research, 2014:916120. DOI:10.1155/2014/916120
Ravindran R., Lakshmanan B., Ravishankar C., Subramanian H. 2008. Prevalence of Linguatula serrata in domestic ruminants in South India. The Southeast Asian Journal of Tropical Medicine and Public Health, 39, 808–812
Rezaei F., Tavassoli M., Javdani M. 2012. Prevalence and morphological characterizations of Linguatula serrata nymphs in camels in Isfahan Province, Iran. Veterinary Research Forum, 3, 61–65.
Shakerian A., Shekarforoush S.S., Ghafari Rad H. 2008. Prevalence of Linguatula serrata nymphs in one-humped camel (Camelus dromedarius) in Najaf-Abad, Iran. Research in Veterinary Science, 84, 243–245. DOI:10.1016/j.rvsc.2007.04.015
Tavassoli M., Tajic H., Dalir-Naghadeh B., Hariri F. 2007. Prevalence of Linguatula serrata nymphs and gross changes of infected mesenteric lymph nodes in sheep in Urmia, Iran. Small Ruminant Research, 72, 73–76. DOI 10.1016/j.smallrumres. 2006.08.013
Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680
Young E. 1975. Pentastomiasis (Armillifer and Linguatula sp.) infestations of wild animals in the Kruger National Park. Journal of the South African Veterinary Association, 46, 335–336
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Ghorashi, S.A., Tavassoli, M., Peters, A. et al. Phylogenetic relationships among Linguatula serrata isolates from Iran based on 18S rRNA and mitochondrial cox1 gene sequences. Acta Parasit. 61, 195–200 (2016). https://doi.org/10.1515/ap-2015-0027
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DOI: https://doi.org/10.1515/ap-2015-0027