Abstract
A human isoform of Collapsin Response Mediator Protein (CRMP) family proteins, CRMP-1, has been identified as a novel invasion suppressor. The aim of this study was to determine CRMP-1 expression pattern in placentas during normal pregnancy and elucidate the clinical significance of CRMP-1 expression in the placentas of women with early-onset preeclamptic pregnancies. We recruited 66 normal healthy pregnant Chinese women and 60 Chinese patients with preeclampsia [early-onset prereclampsia(ePE), n = 30 and late-onset preeclampsia(lPE) n = 30]. Gestational age-matched normal healthy pregnant women were used as controls of early-onset and late-onset preeclampsia, which were 23–33 + 6weeks, n = 18 and control B: 34–40 weeks, n = 20). Quantitative RT-PCR, Western blot analysis and immunohistochemistry were used to analyze the expressions of CRMP-1 in placentas. Expression of CRMP-1 was detected in syncytio- and cytotrophoblasts of all groups using immunohistochemistry. CRMP-1 was most abundantly expressed in syncytiotrophoblasts, moderately in cytotrophoblasts and the intermediate trophoblasts especially in the first trimester. The placental expression of CRMP-1 is particularly striking in the first trimester and decreases throughout pregnancy. There is a significant increase in CRMP-1 expression in the placenta of ePE but not of lPE, as compared to gestational-matched controls. The aberrant upregulation of CRMP-1 expression may link to the mechanism of developing ePE.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Gou WL. Hypertensive disorder in pregnancy). In: Cao ZY, ed. Chinese Obstetrics and Gynecology, 2nd ed. Beijing: People’s Medical Publishing House Co., LTD; 2004:383–342.
Walker JJ. Pre-eclampsia. Lancet. 2000;356(9237):1260–1265.
Meekins JW, Pijnenborg R, Hanssens M, McFadyen IR, van Asshe A. A study of placental bed spiral arteries and Trophoblast invasion in normal and severe preeclamptic pregnancies. Br J Obstet Gynaecol. 1994;101(8):669–674.
Von Dadelszen P, Magee LA, Roberts JM. Subclassification of preeclampsia. Hypertens Pregnancy. 2003;22(2):143–148.
Eastabrook G, Brown M, Sargent I. The origins and end-organ consequence of pre-eclampsia. Best Pract Res Clin Obstet Gynecol. 2011;25(4):435–447.
Huppertz B. Placental origins of preeclampsia: challenging the current hypothesis. Hypertension. 2008;51(4):970–975.
Raymond D, Peterson E. A critical review of early-onset and late-onset preeclampsia. Obstet Gynecol Surv. 2011;66(8):497–506.
Qiao C, Wang C, Zhao J, Liu C, Shang T. Elevated expression of KiSS-1 in placenta of Chinese women with early-onset preeclampsia. PLoS One. 2012;7(11):e48937. doi:10.1371/journal.pone.0048937.
Oudejans CB, van Dijk M, Oosterkamp M, Lachmeijer A, Blankenstein MA. Genetics of preeclampsia: paradigm shifts. Hum Genet. 2007;120(5):607–612.
Crispi F, Llurba E, Domínguez C, Martín-Gallán P, Cabero L, Gratacós E. Predictive value of angiogenic factors and uterine artery Doppler for early-versus late-onset preeclampsia and intrauterine growth restriction. Ultrasound Obstet Gynecol. 2008;31(3):303–309.
Rosslenbroich V, Dai L, Baader SL, Noegel AA, Gieselmann V, Kappler J. Collapsin response mediator protein-4 regulates F-actin bundling. Exp Cell Res. 2005;310(2):434–444.
Quinn CC, Gray GE, Hockfield S. A family of proteins implicated in axon guidance and outgrowth. J Neurobiol. 1999;41(1):158–164.
Shih JY, Lee YC, Yang SC, Hong TM, Huang CY, Yang PC. Collapsin response mediator protein-1: a novel invasion-suppressor gene. Clin Exp Metastasis. 2003;20(1):69–76.
Goshima Y, Nakamura F, Strittmatter P, Strittmatter SM. Collapsin-induced growth cone collapse mediated by an intracellular protein related to UNC-33. Nature. 1995;376(6540):509–514.
Wang LH, Strittmatter SM. Brain CRMP forms heterotetramers similar to liver dihydropyrimidinase. J Neurochem. 1997;69(6):2261–2269.
Fukada M, Watakabe I, Yuasa-Kawada J, et al. Molecular characterization of CRMP5, a novelmember of the collapsin response mediator protein family. J Biol Chem. 2000;275(48):37957–37965.
Arimura N, Inagaki N, Chihara K, et al. Phosphorylation of collapsin response mediator protein-2 by Rho-kinase Evidence for two separate signaling pathways for growth cone collapse. J Biol Chem. 2000;275(31):23973–23980.
Shih JY, Yang SC, Hong TM, et al. Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells. J Natl Cancer Inst. 2001;93(18):1392–1400.
Chen JJ, Peck K, Hong TM, et al. Global analysis of gene expression in invasion by a lung cancer model. Cancer Res. 2001; 61(13):5223–5230.
Gauster M, Moser G, Orendi K, Huppertz B. Factors involved in regulating trophoblast fusion: potential role in the development of preeclampsia. Placenta. 2009;30(suppl A):S49–S54.
Matsuo T, Stauffer JK, Walker RL, Meltzer P, Thiele CJ. Structure and promoter analysis of the human unc-33-like phosphoprotein gene: E-box required for maximal expression in neuroblastoma and myoblasts. J Biol Chem. 2000;275(32):16560–16568.
Inagaki H, Kato Y, Hamajima N, Nonaka M, Sasaki M, Eimoto T. Differential expression of dihydropyrimidinase-related protein genes in developing and adult enteric nervous system. Histochem Cell Biol. 2000;113(1):37–41.
Raper JA. Semaphorins and their receptors in vertebrates and invertebrates. Curr Opin Neurobiol. 2000;10(1):88–94.
Carty DM, Delles C, Dominiczak AF. Novel biomarkers for predicting preeclampsia. Trends Cardiovasc Med. 2008;18(5):186–194.
Wikström AK, Larsson A, Eriksson UJ, Nash P, Olovsson M. Early postpartum changes in circulating pro- and antiangiogenic factors in early-onset and late-onset preeclampsia. Acta Obstetet Gynecol. 2008;87(2):146–153.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Qiao, C., Wang, C., Jin, F. et al. Expression of Collapsin Response Mediator Protein 1 in Placenta of Normal Gestation and Link to Early-Onset Preeclampsia. Reprod. Sci. 22, 495–501 (2015). https://doi.org/10.1177/1933719114549847
Published:
Issue Date:
DOI: https://doi.org/10.1177/1933719114549847