Abstract
Demyelination is the pathological hallmark of multiple sclerosis (MS) lesions. Considering the involvement of hippocampus in MS, we aim to evaluate the effect of vitamin D3 on molecular events in the dorsal hippocampus (CA1 area) following the induction of experimental demyelination in rats. All experiments were carried out on adult male Wistar rats. For demyelination induction, 2 μL lysophosphatidyl choline (LPC) was injected into the CA1 area of rat brain using stereotaxic surgery. Animals were treated with vitamin D3 dissolved in sesame oil at doses of 5 μg/kg intra-peritoneally for 7, 14 and 21 days post receiving LPC. The hippocampus tissue was then removed to measuring the expression of Olig2 (marker of OPCs), GFAP (marker of astrocyte) and Nogo-A (axonal growth inhibitor) genes into lesion. RT-PCR analysis indicated that following hippocampus demyelination Olig2, GFAP and Nogo-A genes expression were significantly increased on days 7, 14 and 21 post lesion. While administration of vitamin D3 for 7, 14 and 21 days post lesion significantly caused a decrease in Olig2, GFAP and Nogo-A genes expression. Our results indicated the positive effect of vitamins D3 on process of remyelination by enhancing oligodendrocyte precursors’ recruitment and decreasing of inhibitory genes such as Nogo-A and GFAP in the context of demyelinating diseases like MS.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Compston, A. and Coles, A., Lancet, 2008, vol. 372, pp. 1502–1517.
Trapp, B.D. and Nave, K.A., Annu. Rev. Neurosci., 2008, vol. 31, pp. 247–269.
Irvine, K.A. and Blakemore, W.F., Brain, 2008, vol. 131, pp. 1464–1477.
Di Bello, I.C., Dawson, M.R., Levine, J.M., and Reynolds, R., J. Neurocytol., 1999, vol. 28, pp. 365–381.
Watanabe, M., Toyama, Y., and Nishiyama, A.., J. Neurosci. Res., 2002, vol. 69, pp. 826–836.
Sofroniew, M.V., Neuroscientist, 2005, vol. 11, pp. 400–407.
Fontoura, P. and Steinman, L.., J. Neurol. Sci., 2006, vol. 245, pp. 201–210.
Magalon, K., Cantarella, C., Monti, G., Cayre, M., and Durbec, P., Eur. J. Neurosci., 2007, vol. 25, pp. 761–771.
Wingerchuk, D.M., Mt. Sinai. J. Med., 2011, vol. 78, pp. 221–230.
Wergeland, S., Torkildsen, Ø., Myhr, K., Aksnes, L., Jarl Mørk, S., and Bø, L., PLoS ONE, 2011, vol. 6, no. 10, pp. 1–8.
Løken-Amsrud, K.I., Holmøy, T., Bakke, S.J., Beiske, A.G., Bjerve, K.S., Bjørnara, B.T., Hovdal, H., Lilleås, F., Midgard, R., Pedersen, T., Benth, J.S., Sandvik, L., Torkildsen, O., Wergeland, S., and Myhr, K.M., Neurology, 2012, vol. 79, pp. 267–273.
Simpson, S., Jr., Taylor, B., Blizzard, L., Ponsonby, A.L., Pittas, F., Tremlett, H., Dwyer, T., Gies, P., and van der Mei, I., Ann. Neurol., 2010, vol. 68, pp. 193–203.
Myhr, K.M.., J. Neurol. Sci., 2009, vol. 286, pp. 104–108.
Soilu-Hanninen, M., Aivo, J., Lindstrom, B.M., Elovaara, I., Sumelahti, M.L., Farkkila, M., Tienari, P., Atula, S., Sarasoja, T., Herrala, L., Keskinarkaus, I., Kruge, J., Kallio, T., Rocca, M.A., and Filippi, M.., J. Neurol. Neurosurg. Psychiatry, 2012, vol. 83, pp. 565–571.
Geurts, J.J., Bo, L., Roosendaal, S.D., Hazes, T., Daniëls, R., Barkhof, F., Witter, M.P., Huitinga, I., and van der Valk, P.., J. Neuropathol. Exp. Neurol., 2007, vol. 66, no. 9, pp. 819–827.
Garcion, E., Sindji, L., Nataf, S., Brachet, P., Darcy, F., and Montero-Menei, C.N., Acta. Neuropathol., 2003, vol. 105, no. 5, pp. 438–448.
Mosayebi, G., Ghazavi, A., and Payani, M.A.., J. Iran. Univ. Med. Sci., 2006, vol. 13, no. 52, pp. 189–196.
Mozafari, S., Javan, M., Sherafat, M., Mirnajafi-Zadeh, J., Heibatollahi, M., Pour Beiranvand, S., Tiraihi, T., and Ahmadiani, A., Neuromol. Med., 2011, vol. 13, pp. 138–150.
Sherafat, M., Javan, M., Mozafari, S., Mirnajafi-Zadeh, J., and Motamedi, F., Neurochem. Res., 2011, vol. 36, pp. 1887–1895.
Majlessi, N., Kadkhodaee, M., Parviz, M., and Naghdi, N., Brain. Res., 2003, vol. 963, pp. 244–251.
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K., Short Protocols in Molecular Biology, New York, NY: John Wiley, 1992.
Khezri, S., Javan, M., Goudarzvand, M., Semnanian, S., and Baharvand, H., J Mol. Neurosci., 2013, vol. 51, no. 2, pp. 298–306.
Tarbali, S. and Khezri, S., J. Neurol. Sci. [Turk.], 2015, vol. 32, no. 3, pp. 482–493.
Baas, D., Prufer, K., Ittel, M.E., Kuchler-Bopp, S., Labourdette, G., Sarlieve, L.L., and Brachet, P., Glia, 2000, vol. 31, pp. 59–68.
Clemens, T.L., Garrett, K.P., Zhou, X.Y., Pike, J.W., Haussler, M.R., and Dempster, D.W., Endocrinol., 1988, vol. 122, no. 4, pp. 1224–1230.
Langub, M.C., Herman, J.P., Malluche, H.H., and Koszewski, N.J., Neurosci., 2001, vol. 104, pp. 49–56.
Hayes, C.E., Proc. Nutr. Soc., 2000, vol. 59, pp. 531–534.
Makinodan, M., Tatsumi, K., Okuda, H., Manabe, T., Yamauchi, T., Noriyama, Y., Kishimoto, T., and Wanaka, A., Neurochem. Int., 2008, vol. 53, pp. 374–381.
Murray, K. and Lledo, P.M., Pathol. Biol., 2006, vol. 54, pp. 266–269.
Nishiyama, A., Lin, X.H., Giese, N., Heldin, C.H., and Stallcup, W.B.., J. Neurosci. Res., 1996, vol. 43, pp. 299–314.
Fancy, S.P., Zhao, C., and Franklin, R.J., Mol. Cell. Neurosci., 2004, vol. 27, pp. 247–254.
Franklin, R.J., Nat. Rev. Neurosci., 2002, vol. 3, pp. 705–714.
Liu, Z., Hu, X., Ca, J., Liu, B., Peng, X., Wegner, M., and Qiu, M., Dev. Biol., 2007, vol. 302. pp. 683–693.
Ligon, K.L., Fancy, S.P.J., Franklin, R.J.M., and Rowitch, D.H., Glia, 2006a, vol. 54, pp. 1–10.
Ligon, K.L., Kesari, S., Kitada, M., Sun, T., Arnett, H. A., Alberta, J.A., Anderson, D.J., Stiles, C.D., and Rowitch, D.H., Proc. Natl. Acad. Sci. U S A, 2006b, vol. 103, pp. 7853–7858.
Dehghan, S., Javan, M., Pourabdolhossein, F., Mirnajafi-Zadeh, J., and Baharvand, H.., J. Mol. Neurosci., 2012, vol. 48, pp. 77–85.
van Halteren, A.G.S., Tysma, O.M., van Etten, E., Mathieu, C., and Roep, B.O., J. Autoimmun., 2004, vol. 23, no. 3, pp. 233–239.
Smith, M.E., and Eng, L.F.., J. Neurosci. Res., 1987, vol. 18, pp. 203–208.
Oblinger, M.M. and Singh, L.D., Int. J. Dev. Neurosci., 1993, vol. 11, pp. 149–156.
Zaaraoui, W., Deloire, M., Merle, M., Girard, C., Raffard, G., Biran, M., Inglese, M., Petry, K.G., Gonen, O., Brochet, B., Franconi, J.M., and Dousset, V., MAGMA, 2008, vol. 21, pp. 357–362.
Spach, K.M., Pedersen, L.B., Nashold, F.E., Kayo, T., Yandell, B.S., Prolla, T. A., and Hayes, C.E., Physiol. Genomics, 2004, vol. 18, pp. 141–151.
Koutsoudakia, P., Skripuletz, T., Gudia, V., Moharregh-Khiabania, D., Hildebrandt, H., Trebst, C., and Stangel, M., Neurosci. Lett., 2009, vol. 451, pp. 83–88.
Nystad, A.E., Wergeland, S., Aksnes, L., Myhr, K.M., Bø, L., and Torkildsen, Ø., APMIS, 2014, vol. 122, pp. 1178–1186.
Kubes, M., Cordier, J., Glowinski, J., Girault, J.A., and Chneiweiss, H., J. Neurochem., 1998, vol. 71, pp. 1307–1314.
Vallano, M.L., Beaman-Hall, C.M., Mathur, A., and Chen, Q., Glia, 2000, vol. 30, pp. 154–164.
Fawcett, J.W. and Asher, R.A., Brain. Res. Bull., 1999, 49, pp. 377–391.
Chen, M.S., Huber, A.B., van der Haar, M.E., Frank, M., Schnell, L., Spillmann, A.A., Christ, F., and Schwab, M.E., Nature, 2000, vol. 403, pp. 434–439.
Wang, X., Chun, S.J., Treloar, H., Vartanian, T., Greer, C.A., and Strittmatter, S.M., J. Neurosci., 2002, vol. 22, no. 13, pp. 5505–5515.
Satoh, J., Onoue, H., Arima, K., and Yamamura, T.., J. Neuropathol. Exp. Neurol., 2005, vol. 64, no. 2, pp. 129–138.
Meier, S., Brauer, A.U., Heimrich, B., Schwab, M., Nitsch, R., and Savaskan, N., FASEB. J., 2003, vol. 17, pp. 1153–1155.
Fontoura, P., Ho, P. P., DeVoss, J., Zheng, B., Lee, B.J., Kidd, B.A., Garren, H., Sobel, R.A., Robinson, W.H., Tessier-Lavigne, M., and Steinman, L., J. Immunol., 2004, vol. 173, pp. 6981–6992.
Karnezis, T., Mandemakers, W., McQualter, J.L., Zheng, B., Ho, P.P., Jordan, K.A., Murray, B.M., Barres, B., Tessier-Lavigne, M., and Bernard, C.C., Nat. Neurosci., 2004, vol. 7, pp. 736–744.
Goudarzvand, M., Javan, M., Mirnajafi-Zadeh, J., Mozafari, S., and Tiraihi, T., Cell. Mol. Neurobiol., 2010, vol. 30, pp. 289–299.
Smolders, J., Moen, S.M., Damoiseaux, J., Huitinga, I., and Holmøy, T.., J. Neurol. Sci., 2011, vol. 311, pp. 37–43.
Kalueff, A.V., Minasyan, A., Keisala, T., Kuuslahti, M., Miettinen, S., and Tuohimaa, P., Neurol. Disord. Drug. Targets, 2006, vol. 5, pp. 363–371.
Neveu, I., Naveilhan, P., Baudet, C., Brachet, P., and Metsis, M., Neuroreport., 1994, vol. 6, no. 1, pp. 124–126.
Kang, H. and Schuman, E.M., Neurosci, Lett., 2000, vol. 282, no. 3, pp. 41–44.
Author information
Authors and Affiliations
Corresponding author
Additional information
The article is published in the original.
Rights and permissions
About this article
Cite this article
Tarbali, S., Khezri, S. & Rahmani, F. Analysis of molecular events associated with adult rat dorsal hippocampus demyelination following treatment with vitamin D3. Neurochem. J. 11, 95–103 (2017). https://doi.org/10.1134/S1819712416040139
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1819712416040139