Abstract
Biodegradable polyethylene oxide (PEO)/chitosan (CS)/poly(ε-caprolactone) (PCL)/olive oil composite nanofibers were fabricated by electrospinning process. The prepared nanofibers were characterized using SEM and FTIR analysis. A response surface methodology based on Box-Behnken design (BBD) was used to predict the average diameter of electrospun nanofibers based on electrospinning parameters including voltage, flow rate and tip-collector distance. The optimum experimental average diameter of electrospun nanofibers was found to be 86 nm which was in good agreement with the predicted value by the BBD analysis (88 nm). In vitro release of olive oil incorporated PEO/CS/PCL/olive oil nanofibers demonstrated a rapid release of olive oil during the first 3 h which enhanced gradually afterwards. Good attachment, spreading, cell proliferation, as well as nontoxic behavior of PEO/CS/PCL/olive oil nanofibrous scaffolds on HDF fibroblast cells were proved by cytotoxicity studies. Furthermore, the high antibacterial activity of PEO/CS/PCL/olive oil composite nanofibers against Gram-negative bacteria E. coli and Gram-positive S. aureus was observed. This study suggests that the prepared PEO/CS/PCL/olive oil composite nanofibrous scaffolds could be used as an ideal patch for wound dressing applications.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
I. S. Chronakis, J. Mater. Proc. Technol., 167, 283 (2005).
A. J. Meinel, O. Germershaus, T. Luhmann, H. P. Merkle, and L. Meinel, Eur. J. Pharm. Biopharm., 81, 1 (2012).
P. Nakielski, T. Kowalczyk, K. Zembrzycki, and T. A. Kowalewski, J. Biomed. Res. Part B, 103, 282 (2015).
J. S. Im, J. Yun, Y. M. Lim, H. I. Kim, and Y. S. Lee, Acta Biomater., 6, 102 (2010).
B. Song, C. Wu, and J. Chang, J. Biomed. Res. Part B, 100, 2178 (2012).
Y. N. Lin, K. M. Chang, S. Jeng, P. Y. Lin, and R. Q. Hsu, J. Mater. Sci.-Mater. Med., 22, 571 (2010).
J. Venugopal, S. Low, A. T. Choon, and S. Ramakrishna, J. Biomed. Res. Part B, 84, 34 (2010).
P. C. Caracciolo, V. Thomas, Y. K. Vohra, F. Buffa, and G. A. Abraham, J. Mater. Sci.-Mater. Med., 20, 2129 (2009).
C. Xu, C. Lei, L. Meng, C. Wang, and Y. Song, J. Biomed. Res. Part B, 100, 1435 (2012).
H. Zhou, A. H. Touny, and S. B. Bhaduri, J. Mater. Sci. Mater. Med., 22, 1183 (2011).
V. Leung, R. Hartwell, S. S. Elizei, H. Yang, A. Ghahary, and F. Ko, J. Biomed. Res. Part B, 102, 508 (2014).
K. T. Shalumon, K. H. Anulekha, V. Sreeja, S. V. Nair, K. P. Nair, and R. J. Chennazhi, Int. J. Biol. Macromol., 49, 247 (2011).
D. S. Katti, K. W. Robinson, F. K. Ko, and C. T. Laurencin, J. Biomed. Res. Part B, 70, 286 (2004).
S. Y. Gu, Z. M. Wang, J. Ren, and C. Y. Zhang, Mater. Sci. Eng. C, 29, 1822 (2009).
T. J. Sill and H. A. Recum, Biomaterials, 29, 1989 (2008).
Y. Zhang, C. T. Lim, S. Ramakrishna, and Z. Huang, J. Mater. Sci.-Mater. Med., 16, 933 (2005).
R. Salehi, M. Irani, M. Eskandani, K. Nowruzi, S. Davaran, I. Haririan, Int. J. Polym. Mater. Polym. Biomat., 63, 609 (2014).
K. T. Shalumon, N. S. Binulal, N. Selvamurugan, S. V. Nair, M. Deepthy, T. Furuike, H. Tamura, and R. Jayakumar, Carbohydr. Polym. 77, 863 (2009).
U. S. Sajeev, A. K. Anoop, M. Deepthy, and S. V. Nair, Bull. Mater. Sci., 31, 343 (2008).
N. S. Binulal, M. Deepthy, N. Selvamurugan, K. T. Shalumon, S. Suja, M. Ullas, R. Jayakumar, and S. V. Nair, Tissue Eng. Part A, 16, 393 (2010).
Y. T. Jia, J. Gong, X. H. Gu, H. Y. Kim, J. Dong, and X. Y. Shen, Carbohydr. Polym., 67, 403 (2007).
K. T. Shalumon, K. H. Anulekha, C. M. Girish, S. V. Prasanth, and R. J. Nair, Carbohydr. Polym., 80, 413 (2010).
Y. S. Zhou, D. Z. Yang, and J. Nie, Chinese Chem. Lett., 18, 118 (2007).
P. Zahedi, I. Rezaeian, S. O. RanaeiSiadat, S. H. Jafari, and P. Supaphol, Polym. Adv. Technol., 21, 77 (2010).
C. K. S. Pillai and C. P. Sharma, Biomater. Artif. Organs, 22, 179 (2009).
X. Y. Li, X. Y. Kong, S. Shi, X. H. Wang, G. Guo, F. Luo, X. Zhao, Y. Q. Wei, and Z. Y. Qian, Carbohydr. Polym., 82, 904 (2010).
J. Han, T.-X. Chen, C. J. B. White, and L. M. Zhu, Int. J. Pharm., 382, 215 (2009).
T. Wang, X. K. Zh, X. T. Xue, and D. Y. Wu, Carbohydr. Polym., 88, 75 (2012).
R. Gurfinkel, M. Palivatkel-Naima, R. Gleisinger, L. Rosenb, and J. Singer, Am. J. Emerg. Med., 30, 79 (2012).
Z. X. Cai, X. M. Mo, K. H. Zhang, L. P. Fan, A. L. Yin, C. L. He, and H. S. Wang, Int. J. Mol. Sci., 11, 3529 (2010).
S. Pandamooz, A. Hadipour, H. Akhavan-Niaki, M. Pourghasem, Z. Abedian, A. M. Ardekani, M. Golpour, M. H. Zuhair, and A. Mostafazadeh, Biotechnol. Appl. Biochem., 59, 254 (2012).
T. Mosmann, J. Immunol. Methods, 65, 55 (1983).
C. Srisitthiratkul, V. Pongsorrarith, and N. Intasanta, Appl. Surf. Sci., 257, 8850 (2011).
L. D. Tijing, M. T. G. Ruelo, A. Amarjargal, H. R. Pant, C.-H. Park, and C. S. Kim, Mater. Chem. Phys., 134, 557 (2012).
Y. Park, I. H. Lee, and G. N. Bea, J. Ind. Eng. Chem., 14, 707 (2008).
S. H. Tan, R. Inai, M. Kotaki, and S. Ramakrishna, Polymer, 46, 6128 (2005).
N. Sultana, M. I. Hassan, and M. M. Lim, “Composite Synthetic Scaffolds for Tissue Engineering and Regenerative Medicine”, pp.13–24, Springer International Publishing, Berlin, 2015.
S. Sarmila, S. Abhishek, N. Rajashree, A. R. Phani, and P. L. Nayak, Carbohydr. Polym., 80, 413 (2009).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zarghami, A., Irani, M., Mostafazadeh, A. et al. Fabrication of PEO/chitosan/PCL/olive oil nanofibrous scaffolds for wound dressing applications. Fibers Polym 16, 1201–1212 (2015). https://doi.org/10.1007/s12221-015-1201-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12221-015-1201-8