Abstract
In order to investigate the culture characteristics of two indoor intensive Litopenaeus vannamei farming modes, recirculating aquaculture system (RAS) and water exchange system (WES), this study was carried out to analyze the water quality and nitrogen budget including various forms of nitrogen, microorganism and chlorophyll-a. Nitrogen budget was calculated based on feed input, shrimp harvest, water quality and renewal rate, and collection of bottom mud. Input nitrogen retained in shrimp was 23.58% and 19.10% respectively for WES and RAS, and most of nitrogen waste retained in water and bottom mud. In addition, most of nitrogen in the water of WES was TAN (21.32%) and nitrite (15.30%), while in RAS was nitrate (25.97%), which means that more than 76% of ammonia and nitrite were removed. The effect of microalgae in RAS and WES was negligible. However, bacteria played a great role in the culture system considering the highest cultivable cultivable bacterial populations in RAS and WES were 1.03×1010 cfu mL−1 and 2.92×109 cfu mL−1, respectively. Meanwhile the proportion of bacteria in nitrogen budget was 29.61% and 24.61% in RAS and WES, respectively. RAS and WES could realize shrimp high stocking culture with water consuming rate of 1.25 m3 per kg shrimp and 3.89 m3 per kg shrimp, and power consuming rates of 3.60 kw h per kg shrimp and 2.51 kw h per kg shrimp, respectively. This study revealed the aquatic environment and nitrogen budget of intensive shrimp farming in detail, which provided the scientific basis for improving the industrial shrimp farming.
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References
Ai, Q., Mai, K., Zhang, W., Xu, W., Tan, B., Zhang, C., and Li, H., 2007. Effects of exogenous enzymes (phytase, non-starch polysaccharide enzyme) in diets on growth, feed utilization, nitrogen and phosphorus excretion of Japanese seabass, Lateolabrax japonicus. Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology, 147: 502–508.
APHA, 1981. Standard Methods for the Examination of Water and Wastewater. APHA American Public Health Association.
Attramadal, K. J. K., Salvesen, I., Xue, R., Oie, G., Storseth, T. R., Vadstein, O., and Olsen, Y., 2012. Recirculation as a possible microbial control strategy in the production of marine larvae. Aquacultural Engineering, 46: 27–39.
Bendschneider, K., and Robinson, R. J., 1952. A new spectrophotometric method for the determination of nitrite in sea water. Journal of Marine Research, 2: 87–96.
Briggs, M. R. P., and Fvnge-Smith, S. J., 1994. A nutrient budget of some intensive marine shrimp ponds in Thailand. Aquaculture Research, 25: 789–811.
CFSY, 2016. China Fishery Statistical Yearbook. China Agriculture Publishing House, Beijing.
Chen, Y., Chen, K., and Hu, Y., 2006. Discussion on possible error for phytoplankton chlorophyll-a concentration analysis using hot-ethanol extraction method. Journal of Lake Sciences, 5: 550–552.
Colson, V., Sadoul, B., Valotaire, C., Prunet, P., Gaume, M., and Labbe, L., 2015. Welfare assessment of rainbow trout reared in a Recirculating Aquaculture System: Comparison with a Flow-Through System. Aquaculture, 436: 151–159.
Dierberg, F. E., and Kiattisimkul, W., 1996. Issues, impacts, and implications of shrimp aquaculture in Thailand. Environmental Management, 20: 649–666.
d’Orbcastel, E. R., Blancheton, J. P., and Aubin, J., 2009a. Towards environmentally sustainable aquaculture: Comparison between two trout farming systems using Life Cycle Assessment. Aquacultural Engineering, 40: 113–119.
d’Orbcastel, E. R., Blancheton, J. P., and Belaud, A., 2009b. Water quality and rainbow trout performance in a Danish Model Farm recirculating system: Comparison with a flow through system. Aquacultural Engineering, 40: 135–143.
d’Orbcastel, E. R., Ruyet, P. L., J., Bayon, N. L, and Blancheton, J. P., 2009c. Comparative growth and welfare in rainbow trout reared in recirculating and flow through rearing systems. Aquacultural Engineering, 40: 79–86.
Ferguson, S. J., 1994. Denitrification and its control. Antonie van Leeuwenhoek, 66: 89–110.
Ge, H., Li, J., Chang, Z., Chen, P., Shen, M., and Zhao, F., 2016. Effect of microalgae with semicontinuous harvesting on water quality and zootechnical performance of white shrimp reared in the zero water exchange system. Aquacultural Engineering, 72: 70–76.
Gelfand, I., Barak, Y., Even-Chen, Z., Cytryn, E., van Rijn, J., Krom, M. D., and Neori, A., 2003. A novel zero discharge intensive Seawater recirculating system for the culture of marine fish. Journal of the World Aquaculture Society, 34: 344–358.
Heisterkamp, I. M., Schramm, A., de Beer, D., and Stief, P., 2016. Direct nitrous oxide emission from the aquacultured Pacific white shrimp (Litopenaeus vannamei). Applied and Environmental Microbiology, 82: 4028–4034.
Hobbie, J. E., Daley, R. J., and Jasper, S., 1977. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Applied and Environmental Microbiology, 33: 1225–1228.
Holl, C. M., Glazer, C. T., and Moss, S. M., 2011. Nitrogen stable isotopes in recirculating aquaculture for super-intensive shrimp production: Tracing the effects of water filtration on microbial nitrogen cycling. Aquaculture, 311: 146–154.
Jackson, C., Preston, N., Thompson, P. J., and Burford, M., 2003. Nitrogen budget and effluent nitrogen components at an intensive shrimp farm. Aquaculture, 218: 397–411.
Kolarevic, J., Baeverfjord, G., Takle, H., Ytteborg, E., Reiten, B. K. M., Nergard, S., and Terjesen, B. F., 2014. Performance and welfare of Atlantic salmon smolt reared in recirculating or flow through aquaculture systems. Aquaculture, 432: 15–25.
Kuhn, D. D., Smith, S. A., Boardman, G. D., Angier, M. W., Marsh, L., and Flick Jr., G. J., 2010. Chronic toxicity of nitrate to Pacific white shrimp, Litopenaeus vannamei: Impacts on survival, growth, antennae length, and pathology. Aquaculture, 309: 109–114.
Lee, S., and Fuhrman, J. A., 1987. Relationships between biovolume and biomass of naturally derived marine bacterioplankton. Applied and Environmental Microbiology, 53: 1298–1303.
Lightner, D. V., and Redman, R., 1998. Shrimp diseases and current diagnostic methods. Aquaculture, 164: 201–220.
Lin, Y. C., and Chen, J. C., 2001. Acute toxicity of ammonia on Litopenaeus vannamei Boone juveniles at different salinity levels. Journal of Experimental Marine Biology and Ecology, 259: 109–119.
Lin, Y. C., and Chen, J. C., 2003. Acute toxicity of nitrite on Litopenaeus vannamei (Boone) juveniles at different salinity levels. Aquaculture, 224: 193–201.
Loh, J. Y., 2017. The role of probiotics and their mechanisms of action: An aquaculture perspective. World Aquaculture, 19–23.
Lue, S., Wang, X., and Han, B., 2009. A field study on the conversion ratio of phytoplankton biomass carbon to chlorophyll-a in Jiaozhou Bay, China. Chinese Journal of Oceanology and Limnology, 27: 793–805.
Martins, C. I. M., Eding, E. H., Verdegem, M. C. J., Heinsbroek, L. T. N., Schneider, O., Blancheton, J. P., d’Orbcastel, E. R., and Verreth, J. A. J., 2010. New developments in recirculating aquaculture systems in Europe: A perspective on environmental sustainability. Aquacultural Engineering, 43: 83–93.
Mei, Z. P., Finkel, Z. V., and Irwin, A. J., 2011. Phytoplankton growth allometry and size-dependent C:N stoichiometry revealed by a variable quota model. Marine Ecology Progress Series, 434: 29–43.
Muthuwan, V., 1991. Nutrient budget and water quality in intensive marine shrimp culture ponds. Master thesis. Asian Institute of Technology, Bangkok.
Naylor, R. L., Goldburg, R. J., Mooney, H., Beveridge, M., Clay, J., Folke, C., Kautsky, N., Lubchenco, J., Primavera, J., and Williams, M., 1998. Nature’s subsidies to shrimp and salmon farming. Science, 282: 883–884.
Oppenheimer, C. H., 1952. The growth and viability of sixtythree species of marine bacteria as influenced by hydrostatic pressure. Journal of Marine Research, 11: 10–18.
Otoshi, C. A., Arce, S. M., and Moss, S. M., 2003. Growth and reproductive performance of broodstock shrimp reared in a biosecure recirculating aquaculture system versus a flowthrough pond. Aquacultural Engineering, 29: 93–107.
Peres, H., and Oliva-Teles, A., 2006. Effect of the dietary essential to non-essential amino acid ratio on growth, feed utilization and nitrogen metabolism of European sea bass (Dicentrarchus labrax). Aquaculture, 256: 395–402.
Perez-Velazquez, M., Gonzalez-Felix, M. L., Gomez-Jimenez, S., Davis, D. A., and Miramontes-Higuera, N., 2008. Nitrogen budget for a low-salinity, zero-water exchange culture system: II. Evaluation of isonitrogenous feeding of various dietary protein levels to Litopenaeus vannamei (Boone). Aquaculture Research, 39: 995–1004.
Pfeffer, C. S., Hite, M. F., and Oliver, J. D., 2003. Ecology of Vibrio vulnificus in estuarine waters of eastern North Carolina. Applied and Environmental Microbiology, 69: 3526–3531.
Piedrahita, R. H., 2003. Reducing the potential environmental impact of tank aquaculture effluents through intensification and recirculation. Aquaculture, 226: 35–44.
Rappé, M. S., and Giovannoni, S. J., 2003. The uncultured microbial majority. Annual Reviews in Microbiology, 57: 369–394.
Rosenthal, H., Castell, J., Chiba, K., Forster, J., Hilge, V., Hogendoorn, H., Mayo, R., Muir, J., Murray, K., and Petit, J., 1986. Flow-through and recirculation systems. EIFAeC, 100.
Samocha, T., Fricker, J., Ali, A., Shpigel, M., and Neori, A., 2015. Growth and nutrient uptake of the macroalga Gracilaria tikvahiae cultured with the shrimp Litopenaeus vannamei in an Integrated Multi-Trophic Aquaculture (IMTA) system. Aquaculture, 446: 263–271.
Summerfelt, S., Sharrer, M., Gearheart, M., Gillette, K., and Vinci, B., 2009. Evaluation of partial water reuse systems used for Atlantic salmon smolt production at the White River National Fish Hatchery. Aquacultural Engineering, 41: 78–84.
Tal, Y., Schreier, H. J., Sowers, K. R., Stubblefield, J. D., Place, A. R., and Zohar, Y., 2009. Environmentally sustainable landbased marine aquaculture. Aquaculture, 286: 28–35.
Thakur, D. P., and Lin, C. K., 2003. Water quality and nutrient budget in closed shrimp (Penaeus monodon) culture systems. Aquacultural Engineering, 27: 159–176.
Thoman, E. S., Ingall, E. D., Davis, D. A., and Arnold, C. R., 2001. A nitrogen budget for a closed, recirculating mariculture system. Aquacultural Engineering, 24: 195–211.
Thompson, F. L., Iida, T., and Swings, J., 2004. Biodiversity of vibrios. Microbiology and Molecular Biology Reviews, 68: 403–431.
Turner, J. W., Good, B., Cole, D., and Lipp, E. K., 2009. Plankton composition and environmental factors contribute to Vibrio seasonality. Isme Journal, 3: 1082–1092.
Verdegem, M. C. J., Bosma, R. H., and Verreth, J. A. J., 2006. Reducing water use for animal production through aquaculture. International Journal of Water Resources Development, 22: 101–113.
Wang, Y., Kong, L. J., Li, K., and Bureau, D. P., 2007. Effects of feeding frequency and ration level on growth, feed utilization and nitrogen waste output of cuneate drum (Nibea miichthioides) reared in net pens. Aquaculture, 271: 350–356.
Weiler, R., 1979. Rate of loss of ammonia from water to the atmosphere. Journal of the Fisheries Board of Canada, 36: 685–689.
Acknowledgements
This study was supported by the China Agriculture Research System (No. CARS-47), the Taishan Industrial Leader Talent Project of Shandong Province (No. LJNY 2015002) and the Aoshan Innovation Project of Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02).We thank the manager and staff of the shrimp farm for providing experimental site, offering management data and the facilities.
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Chen, Z., Ge, H., Chang, Z. et al. Nitrogen Budget in Recirculating Aquaculture and Water Exchange Systems for Culturing Litopenaeus vannamei. J. Ocean Univ. China 17, 905–912 (2018). https://doi.org/10.1007/s11802-018-3584-9
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DOI: https://doi.org/10.1007/s11802-018-3584-9