Abstract
Objective
To investigate the mechanism of Chinese herbal medicine Dioscorea nipponica for the treatment of monosodium urate crystals-induced gouty arthritis (GA) in rats.
Methods
Sixty male Wistar rats were divided into 6 groups: normal, model, indomethacin and three total saponin (900, 300 and 100 mg/kg) groups. The liver, kidney and serum levels of lysosomal enzymes, antioxidant capacities, and inflammatory factors were measured. In addition, the mRNA and protein levels of the NALP3 inflammasome components in the mononuclear cells of rats’ peripheral blood were analyzed using real-time polymerase chain reaction and Western blotting methods, respectively.
Results
Total saponins groups could reduce the activities of β-galactosidase, β-N acetyl glucosamine enzyme, β-glucuronidase, acid phosphatase, and malonaldehyde as well as the contents of TNF-α, IL-1β and IL-8 (all P<0.05). They could also increase the activities of glutathione peroxidase and total superoxide dismutase (both P<0.05). Further studies showed that total saponins groups of high, middle and low doses could all increase the mRNA and protein levels of caspase-1, adapter apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC) and NALP3 in the mononuclear cells of peripheral blood (all P<0.05).
Conclusion
Dioscorea nipponica may treat GA by regulating lysosomal enzymes, antioxidant capacities and the NALP3 inflammasome.
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Abbreviations
- NALP3: NACHT:
-
neuronal apoptosis inhibitor protein, class 2 transcription activator, heterokaryon incompatibility, and telomerase-associated protein 1
- LRR:
-
leucine-rich repeat
- PYD:
-
(pyrin domain) domains-containing protein 3
References
Singh JA, Reddy SG, Kundukulam J. Risk factors for gout and prevention: a systematic review of the literature. Curr Opin Rheumatol 2011;23:192–202.
Choi HK, Atkinson K, Karlson EW, Willett W, Curhan G. Alcohol intake and risk of incident gout in men: a prospective study. Lancet 2004;263:1277–1281.
Choi HK, Atkinson K, Karlson EW, Willett W, Curhan G. Purin-rich food, dairy and protein intake, and the risk of gout in men. N Engl J Med 2004;350:1093–1103.
Richette P, Bardin T. Gout. Lancet 2010;375:318–328.
Smith HS, Bracken D, Smith JM. Gout: current insights and future perspectives. J Pain 2011;12:1113–1129.
Kummer JA, Broekhuizen R, Everett H, Agostini L, Kuijk L, Martinon F, et al. Inflammasome components NALP1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response. J Histochem Cytochem 2006;55:443–452.
Taniguchi S, Sagar J. Regulatory molecules involved in inflammasome formation with special reference to a key mediator protein, ASC. Semin Immunopathol 2007;29:231–238.
Singh JA. Advances in gout: some answers, more questions. Arthritis Res Ther 2010;12:136.
Hoffman HM, Wanderer AA. Inflamma some and IL-1beta-mediated disorders. Curr Allergy Asthma Rep 2010;10:229–235.
Terkeltaub RA. Clinical practice. Gout. N Engl J Med 2003;349:1647–1655.
Mccarty DJ. Urate crystals, inflammation, and colchicine. Arthritis Rheum 2008;58:S20–S40.
Sabina EP, Rasool MK, Mathew L, Ezilrani P, Indu H. 6-Shogaol inhibits monosodium urate crystal-induced inflammation-an in vivo and in vitro study. Food Chem Toxicol 2010; 48:229–235.
Chinese Pharmacopoeia Commission. The Pharmacopoeia of the Peple’s Republic of China. Beijing: The Chemical Industry Publishing House; 2010:250–251.
Ding XL, Li Q. Research progress of Chinese medicine of Rhizoma Dioscoreae Nipponicae. Chongqing J Res Chin Drugs Herbs (Chin) 2003;2:55–57.
Yu SY, Ji FR, Yu DH, Liu SM. Studies on chromatography fingerprint of Dioscorea nipponica serum. Chin J Exp Tradit Med Form (Chin) 2010;18:81.
Yu SY, Liu Y, Liu SM. Simultaneous determination of the contents of protodioscin, methy protodioscin and pseudo protodioscin in Rhizoma Dioscoreae Nipponicae with RP-HPLC. Chin J Exp Tradit Med Form (Chin) 2012;18:96–98.
Lu F, Liu L, Yu DH, Li XZ, Zhou Q, Liu SM. Therapeutic effect of Rhizoma Dioscoreae Nipponicae on gouty arthritis based on the SDF-1/CXCR4 and p38 mark pathway: an in vivo and in vitro study. Phytother Res 2014;28:280–288.
Zhou Q, Zhang C, Yu DH, Liu SM. Study on uric acid reducing effect of total saponins from Rhizoma Dioscoreae Nipponicae in treating hyperuricemia and in vitro study of its anti-inflammatory effect. Chin J Tradit Chin Med Pharm (Chin) 2013;28:1444–1448.
Zhou Q, Yu DH. Total saponins from Discorea nipponica ameliorate urate excretion in hyperuricemic mice. Planta Med 2014;80:1259–1268.
Xue J, Zhu RQ, Lu F, Liu SM, Zhou Q. Effects of total saponins from Discorea nipponica on the activities of β-galactosidase and β-N acetyl glucosamine enzyme in the liver of rats of gouty arthritis. Acta Chin Med Pharmacol (Chin) 2014;42:47–49.
Zhou Q, Lu F, Liu SM. The mechanism of cryopyrin inflammasome during the attack of gouty arthritis. Chin J Cell Mol Immunol (Chin) 2013;29:444–447.
Pascual E, Batle-Gualda E, Martinez A, Rosas J, Vela P. Synovial fluid analysis for diagnosis of intercritical gout. Ann Intern Med 1999;131:756–759.
Landis RC, Haskard DO. Pathogenesis of crystal-induced inflammation. Curr Rheumatol Rep 2001;3:36–41.
Gupta OP, Sharma N, Chand DA. Sensitive and selective model for evaluating anti-inflammatory activity-papaya latex-induced rat paw inflammation. J Pharmacol Toxicol Meth 1992;28:15–19.
Rassol M, Sabina EP, Lavanya B. Anti-inflammatory effect of Spirulina fusiformis on adjuvant-induced arthritis in mice. Biol Pharm Bull 2006;29:2483–2487.
Ramprasad VR, Shanthi P, Sachdanandam P. Evaluation of antioxidant effect of Semecarpus anacardium Linn. nut extract on the components of immune system in adjuvant arthritis. Vasc Pharmacol 2005;42:179–186.
Biju T, Shabeer MM, Amitha R, Rajendra BP, Suchetha K. Comparative evaluation of serum superoxide dismutase and glutathione levels in periodontally diseased patients: an interventional study. Indian J Dent Res 2014;25:613–616.
Grzanna R, Phan P, Polotsky A. Ginger-an herbal medicinal product with broad anti-inflammatory actions. J Med Food 2005;8:125–132.
Albano E. Free radical mechanisms in immune reactions associated with alcoholic liver disease. Free Radic Biol Med 2002;32:110–114.
Acknowledgments
The first author of this paper is currently working at the postdoctoral research station of Heilongjiang University of Chinese Medicine.
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Zhou Q and Zhang N carried out the experiments, Yu DH contributed to statistical analysis, Zhou Q wrote the manuscript, Lu SM designed the project and supervised the work and provided the facilities for the study. All authors read the manuscript and approved the final version.
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Supported by the National Natural Science Foundation of China (Nos. 81173618, 81403156), China Postdoctoral Science Foundation (No. 2015M57036), Heilongjiang Postdoctoral Special Assisted Fund (Nos. LBH-TZ0520, LBH-Z13191), Scientific Fund of Heilongjiang University of Chinese Medicine (No. 2013bs05) and the Outstanding Innovative Talent Support Programs of Heilongjiang University of Chinese Medicine
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Zhou, Q., Yu, Dh., Zhang, N. et al. Anti-inflammatory Effect of Total Saponin Fraction from Dioscorea nipponica Makino on Gouty Arthritis and Its Influence on NALP3 Inflammasome. Chin. J. Integr. Med. 25, 663–670 (2019). https://doi.org/10.1007/s11655-016-2741-5
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DOI: https://doi.org/10.1007/s11655-016-2741-5