Abstract
The effects of phosphate concentration on plant growth and photosynthetic performance were examined in leaves of Zizania latifolia. Plants were grown for four weeks in a solution containing 0, 0.16, 0.64, and 2.56 mM orthophosphate. The results showed that the highest net photosynthetic rate (P N) was achieved at 0.64 mM orthophosphate, which corresponded to the maximum content of organic phosphorus in leaves. Low phosphorus (low-P) content in the culture solution inhibited plant growth, affecting plant height, leaf length, leaf number, tiller number, and fresh mass of leaf, sheath, culm, root, and total plant. In addition, we observed that low-P (0.16 mM) did not hinder the growth of roots but increased the root:shoot ratio, and significantly decreased the chlorophyll content, P N, stomatal conductance, and transpiration rate, but increased the intercellular CO2 concentration. Additionally, low-P significantly decreased the maximum carboxylation rate of Rubisco, the maximum rate of ribulose-1,5-bisphosphate regeneration, the effective quantum yield of PSII photochemistry, photochemical quenching coefficient, and electron transport rate, but increased the nonphotochemical quenching. However, the maximal quantum yield of PSII photochemistry was not significantly affected by low-P. High phosphorus (2.56 mM) caused only a slight decrease in gas-exchange parameters. Therefore, the decrease in growth of P-deficient Z. latifolia plants could be attributed to the lowered photosynthetic rate.
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Abbreviations
- Chl:
-
chlorophyll
- C i :
-
intercellular CO2 concentration
- E :
-
transpiration rate
- ETR:
-
electron transport rate
- FM:
-
fresh mass
- Fv/Fm :
-
maximal quantum yield of PSII photochemistry
- g s :
-
stomatal conductance
- J max :
-
maximum rate of RuBP regeneration
- NPQ:
-
nonphotochemical quenching
- P:
-
phosphorus
- Pi :
-
inorganic phosphorus
- P N :
-
net photosynthetic rate
- Po :
-
organic phosphorus
- Ptot :
-
total phosphorus
- qP :
-
photochemical quenching coefficient
- V cmax :
-
maximum carboxylation rate of Rubisco
- ΦPSII :
-
effective quantum yield of PSII photochemistry
References
Campbell C.D., Sage R.E.: Interactions between the effects of atmospheric CO2 content and P nutrition on photosynthesis in white lupin (Lupinus albus L.). — Plant Cell Environ. 29: 844–853, 2006.
Chiera J., Thomas J., Rufty T.: Leaf initiation and development in soybean under phosphorus stress. — J. Exp. Bot. 53: 473–481, 2002.
Clarkson D.T., Carvajal M., Henzler T. et al.: Root hydraulic conductance: diurnal aquaporin expression and the effects of nutrient stress. — J. Exp. Bot. 51: 61–70, 2000.
De Groot C.C., Marcelis L.F.M., Van den Boogaard R. et al.: Growth and dry-mass partitioning in tomato as affected by phosphorus nutrition and light. — Plant Cell Environ. 24: 1309–1317, 2001.
De Groot C.C., Van den Boogaard R., Marcelis L.F.M. et al.: Contrasting effects of N and P deprivation on the regulation of photosynthesis in tomato plants in relation to feedback limitation. — J. Exp. Bot. 54: 1957–1967, 2003.
Duchein M.C., Bonicel A., Betsche T.: Photosynthetic net CO2 uptake and leaf phosphate concentrations in CO2 enriched clover (Trifolium subterraneum L.) at three levels of phosphate nutrition. — J. Exp. Bot. 44: 17–22, 1993.
Duff S.M.G., Sarath G., Plaxton W.C.: The role of acid phosphatases in plant phosphorus metabolism. — Physiol. Plantarum 90: 791–800, 1994.
Foyer C., Spencer C.: The relationship between phosphate status and photosynthesis in leaves. Effects on intracellular orthophosphate distribution, photosynthesis and assimilate partitioning. — Planta 167: 369–375, 1986.
Fredeen A.L., Raab T.K., Rao I.M. et al.: Effects of phosphorus nutrition on photosynthesis in Glycine max. — Planta 181: 399–405, 1990.
Fredeen A.L., Rao I.M., Terry N.: Influence of phosphorus nutrition on growth and carbon partitioning in Glycine max. — Plant Physiol. 89: 225–230, 1989.
Fujita K., Okada M., Lei K. et al.: Effect of P-deficiency on photoassimilate partitioning and rhythmic changes in fruit and stem diameter of tomato (Lycopersicon esculentum) during fruit growth. — J. Exp. Bot. 54: 2519–2528, 2003.
Genty B., Briantais J.M., Baker N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. — Biochim. Biophys. Acta 990: 87–92, 1989.
Ghannoum O., Conroy J.P.: Phosphorus deficiency inhibits growth in parallel with photosynthesis in a C3 (Panicum laxum) but not two C4 (P. coloratum and Cenchrus ciliaris) grasses. — Funct. Plant Biol. 34: 72–81, 2007.
Guo Y.P., Chen P.Z., Zhang L.C. et al.: [Phosphorus deficiency stress aggravate photoinhibition of photosynthesis and function of xanthophyll cycle in citrus leaves.] — J. Plant Nutr. Fertil. Sci. 9: 359–363, 2003. [In Chinese]
Hammond J.P., White P.J.: Sucrose transport in the phloem: integrating root responses to phosphorus starvation. — J. Exp. Bot. 59: 93–109, 2008.
Jacob J., Lawlor D.W.: Dependence of photosynthesis of sunflower and maize on phosphate supply, ribulose-1,5-bisphosphate carboxylase/oxygenase activity, and ribulose-1,5-bisphosphate pool size. — Plant Physiol. 98: 801–807, 1992.
Jacob J., Lawlor D.W.: Stomatal and mesophyll limitations of photosynthesis in phosphate deficient sunflower, maize and wheat plants. — J. Exp. Bot. 42: 1003–1011, 1991.
Jiang L.N., Fu J.R., Fu C.H. et al.: [Effects of balance fertilization on yields and quality of jiaobai (Zizania caduciflora L.).] — Acta Agron. Zhejiang. 15: 161–166, 2003. [In Chinese]
Kavanová M., Lattanzi F.A., Grimoldi A.A. et al.: Phosphorus deficiency decreases cell division and elongation in grass leaves. — Plant Physiol. 141: 766–775, 2006.
Krause G.H., Weis E.: Chlorophyll fluorescence and photosynthesis: The Basics. — Annu. Rev. Plant Phys. 42: 313–349, 1991.
Lambers H., Shane M.W., Cramer M.D. et al.: Root structure and functioning for efficient acquisition of phosphorus: matching morphological and physiological traits. — Ann. Bot.-London 98: 693–713, 2006.
Lewis J.D., Griffin K.L., Thomas R.B. et al.: Phosphorus supply affects the photosynthetic capacity of loblolly-pine grown in elevated carbon-dioxide. — Tree Physiol. 14: 1229–1244, 1994.
Li S.C., Hu C.H., Gong J. et al.: [Effects of low phosphorus stress on the chlorophyll fluorescence of different phosphorus use efficient maize (Zea mays L.).] — Acta Agron. Sin. 30: 365–370, 2004. [In Chinese]
Lichtenthaler H.K.: Chlorophylls and carotenoids: Pigments of photosynthetic membranes. — Methods Enzymol. 148: 350–382, 1987.
Linka N., Weber A.P.M.: Intracellular metabolite transporters in plants. — Mol. Plant 3: 21–53, 2010.
Loustau D., Beahim M., Gaudillère J.P. et al.: Photosynthetic responses to phosphorous nutrition in two-year-old maritime pine seedlings. — Tree Physiol. 19: 707–715, 1999.
McMurtrie R.E., Wang Y.P.: Mathematical models of the photosynthetic response of tree stands to rising CO2 concentrations and temperature. — Plant Cell Environ. 16: 1–13, 1993.
Mollier A., Pellerin S.: Maize root system increment and development as influenced by phosphorous deficiency. — J. Exp. Bot. 50: 487–497, 1999.
Moor U., Põldma P., Tõnutare T. et al.: Effect of phosphite fertilization on growth, yield and fruit composition of strawberries. — Sci. Hortic.-Amsterdam 119: 264–269, 2009.
Müller P., Li X.P., Niyogi K.K.: Non-photochemical quenching. A response to excess light energy. — Plant Physiol. 125: 1558–1566, 2001.
Naeem M., Khan M.M.A., Moinuddin I.M. et al.: Phosphorus ameliorates crop productivity, photosynthetic efficiency, nitrogen-fixation, activities of the enzymes and content of nutraceuticals of Lablab purpureus L. — Sci. Hortic. 126: 205–214, 2010.
Nichols D.G., Jones D.L., Beardsell D.V.: The effect of phosphorus on the growth of Grevillea ‘Poorinda firebird’ in the soil-less potting mixture. — Sci. Hortic.-Amsterdam 11: l97–205, 1979.
Pieters A.J., Paul M.J., Lawlor D.W.: Low sink demand limits photosynthesis under Pi deficiency. — J. Exp. Bot. 52: 1083–1091, 2001.
Plesničar M., Kastori R., Petrović N. et al.: Photosynthesis and chlorophyll fluorescence in sunflower (Helianthus annuus L.) leaves as affected by phosphorus nutrition. — J. Exp. Bot. 45: 919–924, 1994.
Radin J.W., Eidenbock M.P.: Hydraulic conductance as a factor limiting leaf expansion of phosphorus-deficient cotton plants. — Plant Physiol. 75: 372–377, 1984.
Radin J.W., Mathews M.A.: Water transport properties of cortical cells in roots of nitrogen- and phosphorus-deficient cotton seedlings. — Plant Physiol. 89: 264–268, 1989.
Radin J.W.: Stomatal responses to water stress and to abscisic acid in phosphorus-deficient cotton plants. — Plant Physiol. 76: 392–394, 1984.
Raghothama K.G.: Phosphate acquisition. — Annu. Rev. Plant Phys. 50: 665–693, 1999.
Rao I.M., Terry N.: Leaf phosphate status, photosynthesis, and carbon partitioning in sugar beet: I. Changes in growth, gas exchange and Calvin cycle enzymes. — Plant Physiol. 90: 814–819, 1989.
Rodríguez D., Andrade F.H., Goudriaan J.: Effects of phosphorus nutrition on tiller emergence in wheat. — Plant Soil 209: 283–295, 1999.
Rodríguez D., Zubillaga M.M., Ploschuk E.L. et al.: Leaf area expansion and assimilate production in sunflower (Helianthus annuus L.) growing under low phosphorus conditions. — Plant Soil 202: 133–147, 1998.
Schachtman D.P., Reid R.J., Ayling S.M.: Phosphorus uptake by plants: from soil to cell. — Plant Physiol. 116: 447–453, 1998.
Shane M.W., McCully M.E., Lambers H.: Tissue and cellular phosphorus storage during development of phosphorus toxicity in Hakea prostrata (Proteaceae). — J. Exp. Bot. 55: 1033–1044, 2004.
Sharkey T.D.: Photosynthesis in intact leaves of C3 plants: Physics, physiology and rate limitations. — Bot. Rev. 51: 53–105, 1985.
Silber A., Ganmore-Neumann R., Ben-Jaacov J.: The response of three Leucadendron cultivars (Proteaceae) to phosphorus. — Sci. Hortic.-Amsterdam 84: 141–149, 2000.
Sugino Y., Miyoshi Y.: The specific precipitation of orthophosphate and some biochemical applications. — J. Biol. Chem. 239: 2360–2364, 1964.
Terry N., Ulrich A.: Effects of phosphorus deficiency on the photosynthesis and respiration of leaves in sugar beet. — Plant Physiol. 51: 43–47, 1973.
Usuda H., Shimogawara K.: Phosphorus deficiency in maize. I. Leaf phosphate status, growth, photosynthesis and carbon partitioning. — Plant Cell Physiol. 32: 497–504, 1991.
Vance C.P., Uhde-Stone C., Allan D.L.: Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. — New Phytol. 157: 423–447, 2003.
Van Kooten O., Snel J.F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology. — Photosynth. Res. 25: 147–150, 1990.
Wu Y.Q., Li Y., Yang J.W.: [Effects of high phosphorus on phosphorus absorption, transportation and use efficiency of tomato.] — Chin. Agr. Sci. Bull. 25: 162–165, 2009. [In Chinese]
Yan N., Wang X.Q., Xu X.F. et al.: Plant growth and photosynthetic performance of Zizania latifolia are altered by endophytic Ustilago esculenta infection. — Physiol. Mol. Plant P. 83: 75–83, 2013a.
Yan N., Xu X.F., Wang Z.D. et al.: Interactive effects of temperature and light intensity on photosynthesis and antioxidant enzyme activity in Zizania latifolia Turcz. plants. — Photosynthetica 51: 127–138, 2013b.
Zhang J.Z., Chu F.Q., Guo D.P. et al.: Cytology and ultrastructure of interactions between Ustilago esculenta and Zizania latifolia. — Mycol. Prog. 11: 499–508, 2012.
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Acknowledgements: This work was supported by the National Natural Science Foundation of China (31372055). The authors thank editors and anonymous reviewers for their valuable comments.
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Yan, N., Zhang, Y.L., Xue, H.M. et al. Changes in plant growth and photosynthetic performance of Zizania latifolia exposed to different phosphorus concentrations under hydroponic condition. Photosynthetica 53, 630–635 (2015). https://doi.org/10.1007/s11099-015-0149-7
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DOI: https://doi.org/10.1007/s11099-015-0149-7