Abstract
The potential toxicity of nanoparticles (NPs) is under debate. Information about TiO2 NPs phytotoxicity is still limited partly due to the different TiO2 NP forms that may be found in the environment. The present work investigated the impact of different TiO2 NPs forms (rutile and anatase) on germination, growth, cell cycle profile, ploidy level, and micronucleus formation in Lactuca sativa (lettuce) and Ocimum basilicum (basil). Seeds were exposed to anatase (ana) or rutile + anatase (rut+ana) at concentrations 5 - 150 mg dm-3 for 5 d and after that different parameters were analyzed. Rut+ana showed high potential to impair germination and growth. On the other hand, ana alone showed a positive influence on seedling growth. Despite that, ana induced severe alterations in cell cycle dynamics. Regarding species, basil was more sensitive to TiO2 NPs cytostatic effects (delay/arrest in G0/G1 phase), whereas in lettuce, TiO2 NPs were more genotoxic (micronucleus formation increase). Finally, we propose that, besides germination and plant growth, cell cycle dynamics and micronucleus formation can be sensitive biomarkers of these NPs.
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Abbreviations
- Ana:
-
anatase
- MN:
-
micronuclei
- NP:
-
nanoparticle
- rut:
-
rutile
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Acknowledgments: Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES) supported S. Silva (SFRH/BPD⁄74299⁄2010) and H. Oliveira (SFRH/BPD/111736/2015) grants from the financing program QREN–POPH/FSE–Tipologia 4.1–Formação Avançada. We want to thank for the for the financial support to CESAM (UID/AMB/50017) and UI QOPNA (FCT UID/QUI/00062/2013), to FCT/MEC through national funds and co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020. This work was also funded by FEDER/COMPET/POCI, POCI-01-0145- FEDER-006958 (UID/AGR/04033/2013).
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Silva, S., Oliveira, H., Silva, A.M.S. et al. The cytotoxic targets of anatase or rutile + anatase nanoparticles depend on the plant species. Biol Plant 61, 717–725 (2017). https://doi.org/10.1007/s10535-017-0733-8
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DOI: https://doi.org/10.1007/s10535-017-0733-8