Abstract
Visceral leishmaniasis (VL) is one of the most severe forms of leishmaniasis which is fatal if left untreated. Sterol biosynthetic pathway in Leishmania is currently being explored for its therapeutic potential. In the present study, we have evaluated the antileishmanial efficacy of mevastatin, a known inhibitor of 3-hydroxy-3-methyl glutaryl-CoA reductase (HMGR) enzyme. Mevastatin inhibited Leishmania donovani promastigotes and intracellular amastigotes with an 50 % inhibitory concentration (IC50) value of 23.8 ± 4.2 and 7.5 ± 1.1 μM, respectively, without exhibiting toxicity towards host cell line. Mevastatin also inhibited recombinant L. donovani HMGR (LdHMGR) enzyme activity with an IC50 value of 42.2 ± 3.0 μM. Kinetic analysis revealed that the inhibition of recombinant LdHMGR activity by mevastatin was competitive with HMG-CoA. Mevastatin-treated parasites exhibited 66 % reduction in ergosterol levels with respect to untreated parasites. Incubation of mevastatin-treated L. donovani promastigotes with ergosterol resulted in revival of cell growth, whereas cholesterol supplementation failed to cause reversal in cell death. To further prove the specificity of mevastatin for HMGR enzyme, HMGR-overexpressing parasites were used which showed almost threefold resistance to mevastatin. It also induced morphological changes in the parasite accompanied by lipid body accumulation. Hence, antileishmanial effect of mevastatin was due to the inhibition of HMGR, which eventually leads to reduction in ergosterol levels and hence parasite death. The present study may have implications in the treatment of visceral form of leishmaniasis.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Leishmania is an obligate pathogenic parasite which causes worldwide human morbidity and mortality. They are transmitted to humans by the bite of the female sandfly of the genus Phlebetomus (Murray et al. 2005). The estimated incidence of leishmaniasis is 0.4 million cases of the visceral leishmaniasis (VL) and 1.2 million cases of the cutaneous form (Alvar et al. 2012). Antimonial compounds remain the drug of choice for the treatment of leishmaniasis despite reports of high toxicity and other severe side effects. Amphotericin B and pentamidine are second-line drugs which are also reported to possess serious side effects (Croft and Olliaro 2011). Miltefosine, an oral drug approved for VL in India, has also shown severe side effects. Till date, there is no vaccine against leishmaniasis, and control of VL is entirely dependent upon chemotherapy which itself is not free from its own drawbacks. Increasing cases of resistance to commonly available drugs has posed a serious problem for the disease treatment. Therefore, identification of novel drug targets and effective drugs with less toxicity and therapeutic switching of available drugs are few strategies being implied to combat leishmaniasis.
Sterol biosynthetic pathway has been extensively explored in trypanosomatids for the development of new drugs (de Souza and Rodrigues 2009; Roberts et al. 2003). Currently, the enzymes of this pathway are also being studied in Leishmania because of its unique disparity with its mammalian counterpart (Dinesh et al. 2014b). In fungi and Leishmania, this pathway produces ergosterol which in host is replaced by cholesterol (Jimenez-Jimenez et al. 2008). In eukaryotic cells, sterols are important components which are responsible for membrane fluidity and permeability. They serve as precursor and regulator of cell cycle and development (Roberts et al. 2003). One of the enzymes which are currently being explored in our laboratory is 3-hydroxy-3-methyl glutaryl-CoA reductase (HMGR) which has statin as one of the well-known inhibitors. Statins are potent inhibitors of cholesterol biosynthesis which are extensively used in the treatment of hyperlipidemia and also considered useful for the prevention of cardiovascular diseases (Taylor et al. 2013). Statins impede cholesterol synthesis by inhibiting HMGR, which is a rate-limiting enzyme in sterol synthesis. Statins have been found to inhibit the growth of many protozoan parasites like Plasmodium falciparum and Trypanosomatidae family such as Trypanosoma cruzi and various Leishmania species (Montalvetti et al. 2000; Pradines et al. 2007; Urbina et al. 1993; Yokoyama et al. 1998). However, recent studies on statins have been widely investigated for their clinical benefits in various diseases like colon, lung, kidney, bone cancer, and central nervous system, as well as diabetes (Liao 2005) apart from exhibiting immunomodulatory effects (Danesh et al. 2003). We had previously demonstrated the antileishmanial potential of inhibitors belonging to statin class of compounds like atorvastatin, simvastatin, and antidepressants like ketanserin and mianserin via targeting HMGR enzyme (Dinesh et al. 2014a, b; Singh et al. 2014).
Mevastatin also known as compactin belongs to the family of statins which are selective inhibitors of HMGR (Fig. 1a). It is the first member of the statin family which is reported as an inhibitor of cholesterol biosynthesis in Penicillum citrinum (Endo et al. 1976). Since the discovery of mevastatin in 1976, various other statins have been propelled by distinct pharmaceutical companies (Korlipara 2002; Teo and Burton 2002). Mevastatin has shown inhibition against P. falciparum parasite in a stage-dependent manner by altering the biosynthesis of dolichol, dolichyl-P (dolichyl phosphate), and isoprenyl-PP (isoprenyl pyrophosphate) species (Couto et al. 1999). Inhibition of mevalonate pathway by mevastatin is reported to induce apoptosis in human myeloma cell line (Shipman et al. 1998). In mice, it was reported to upregulate the endothelial nitric oxide synthase mRNA and protein levels by inhibiting HMGR. It also reduces the cerebral injury in mice at 20 mg/kg dosage (Amin-Hanjani et al. 2001). Furthermore, it has been shown to inhibit the growth of theca-interstitial cells by interfering with mevalonic acid production thus leading to reduction of both basal- and insulin-induced activation (Kwintkiewicz et al. 2006). Considering the above-cited emerging therapeutic uses of mevastatin, we have made an attempt to find out the antileishmanial potential of mevastatin for the first time and have elucidated its mode of action.
Materials and methods
Materials
Mevastatin (2S)-2-methyl-(1S,7S,8S,8aR)-1,2,3,7,8,8a-hexahydro-7-methyl-8-[2-[(2R,4R)-tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl]ethyl]-1-naphthalenyl butanoate), protease inhibitor cocktail, and (R,S)-3-hydroxy-3-methylglutaryl coenzyme A sodium salt hydrate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mevastatin was dissolved in 20 % ethanol, and stock solution of this drug was stored at −20 °C. Fetal bovine serum (FBS) and RPMI-1640 medium were purchased from Gibco/BRL, Life Technologies Scotland, UK.
Parasite and macrophages
Promastigote form of L. donovani wild-type (WT, MHOM/80/IN/Dd8) were cultured at 24 °C in RPMI-1640 HEPES-modified medium supplemented with 0.2 % sodium bicarbonate, 100 μg/mL penicillin, 100 μg/mL streptomycin, 100 μg/mL gentamycin, and 10 % heat-inactivated FBS. The medium was maintained at pH 7.2. Promastigotes, transfected with pspα hygroα shuttle vector and pspα hygroα-LdHMGR episomal overexpression construct, were routinely maintained in 60 μg/mL hygromycin antibiotic as reported earlier (Singh et al. 2014). These mutant parasites were used in all subsequent experiments. THP-1 cells were routinely cultured in RPMI-1640 medium supplemented with 10 % FBS and maintained at 37 °C in a humidified atmosphere with 5 % CO2.
Evaluation of in vitro antileishmanial activity
Drug sensitivity of L. donovani promastigotes was assayed colorimetrically by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay as described earlier (Mosmann 1983). Briefly, 2 × 105 log phase promastigotes of L. donovani were incubated for 48 h and treated with increasing concentrations of mevastatin (10–100 μM) at 24 °C for 48 h. The percentage viability of promastigotes was calculated relatively by considering 100 % viability in untreated promastigotes. The results were expressed as mean ± SD of at least three independent experiments. Miltefosine was used as the control drug.
Effect of mevastatin on intracellular L. donovani amastigotes was assessed via parasite rescue and transformation assay (Jain et al. 2012). Approximately 2 × 105 THP-1 monocytes were differentiated into macrophages by treating with 20 ng/mL of phorbol myristate acetate (PMA). Macrophages were allowed to adhere for 48 h and infected with 2 × 106 L. donovani promastigotes at 37 °C in 5 % CO2 for 24 h. Unadhered parasites were removed by washing with RPMI medium and the internalized parasite form, i.e., amastigotes were treated with mevastatin at different concentrations. Parasite-infected macrophages were lysed and amastigotes were reverted back to the promastigotes by growing under suitable conditions. Finally, MTT assay was performed as described earlier. Miltefosine was used as the standard drug. Assay was performed in triplicates, and results were representative of three independent experiments.
Mammalian cytotoxicity studies
THP-1 monocytes were cultured in RPMI-1640 medium with 10 % FBS at 37 °C in 5 % CO2-humidified incubator. Cells were seeded at 2 × 105 cells in 96-well microplate, differentiated by adding PMA (20 ng/mL), and incubated at 37 °C with 5 % CO2 for 48 h. After incubation, unadhered cells were removed by washing with serum-free medium. The cells were incubated at 37 °C for 48 h in a humidified atmosphere of 5 % CO2 after addition of different concentrations of mevastatin and miltefosine ranging from 10 to 100 μM (10, 20, 50, and 100 μM). The cells were subsequently treated with MTT dye and incubated at 37 °C for 4 h. The cells were then centrifuged at 3,000g, and the pellets were dissolved in DMSO. Viability of the cells was analyzed with a plate reader at a wavelength of 540 nm.
Evaluation of the effect of mevastatin on HMGR activity in total cell lysates of L. donovani promastigotes
The total cell lysate was isolated from L. donovani promastigotes by using freeze–thaw lysis method. Briefly, 1 × 108 cells were seeded and incubated for 48 h. The 50 % inhibitory concentration (IC50) of mevastatin (23 μM) was added, and the cells were further incubated at 24 °C for 48 h. The cells were harvested at 6,000g for 10 min and washed with phosphate buffer pH 7.4. The cell pellet was resuspended in lysis buffer [50 mM KH2PO4 (pH 7.2), 1 mM DTT, 2 mM PMSF, and 0.5 mg/mL leupeptin] and incubated on ice for 10 min. The cells were lysed by repeated freeze–thaw cycles in liquid nitrogen. The supernatant was collected after centrifugation at 12,000g for 30 min at 4 °C from untreated and mevastatin-treated samples to analyze HMGR activity by a method described earlier (Hurtado-Guerrrero et al. 2002). Protein amount was determined by bicinchoninic acid method using bovine serum albumin as the protein standard (Smith et al. 1985).
Evaluation of the effect of mevastatin on recombinant HMGR activity
Recombinant L. donovani HMGR was overexpressed in Escherichia coli and purified using Nickel affinity chromatography (Dinesh et al. 2014b). In order to evaluate the effect of mevastatin on recombinant LdHMGR enzyme, activity of purified enzyme was assayed in the presence of increasing concentrations of mevastatin. Briefly, the reaction mixture contained 50 mM KH2PO4, 50 mM KCl, 5 mM DTT, 1 mM EDTA, 0.27 mM NADPH, 0.27 mM HMG-CoA, and recombinant enzyme in a final volume of 200 μL at pH 7.2. The HMG-CoA-dependent oxidation of NADPH was monitored at 340 nm (Singh et al. 2014).
For inhibition kinetics, HMGR enzyme was preincubated with inhibitor for 5 min. Reaction was monitored at 340 nm at regular time intervals until the rate of reaction became constant to detect the disappearance of NADPH. In order to determine the rate of reaction, different concentrations of the substrate HMG-CoA (6.25 to 1,200 μM) were added to the enzyme in the presence of 42 and 100 μM of mevastatin. Double reciprocal plots were generated for the calculation of various kinetic parameters. The parameters were evaluated by nonlinear regression using Michaelis-Menten equation. One unit (1 U) of HMGR is defined as the amount of enzyme which catalyzes the oxidation of 1 μmol of NADPH per minute.
Effect of mevastatin on ergosterol levels of L. donovani promastigotes
Neutral lipids were isolated and separated by high-pressure liquid chromatography (HPLC) as previously described (Singh et al. 2014). Approximately 1 × 106 L. donovani promastigotes were incubated at 24 °C for 48 h and treated with mevastatin for 48 h. Promastigotes were harvested, washed with phosphate-buffered saline (PBS), and resuspended in a mixture of methanol/chloroform/water and incubated on ice for 1 h. After centrifugation, the supernatant was collected and a mixture of water/chloroform (1:1 v/v) was added. The mixture was vortexed for 45 s and centrifuged at 3,000g. The organic layer containing sterols was collected. Samples were prepared and subjected to HPLC method with a C18 column (Phenomenex) equipped with UV detector wavelength of 282 nm. Estimation of ergosterol was carried out by HPLC as described previously (Ng et al. 2008). Ergosterol (Sigma) was used as a standard.
Exogenous supplementation of sterols for reversal of mevastatin-mediated growth inhibition
Reversal of mevastatin-mediated growth inhibition of L. donovani promastigotes was studied as described previously (Dinesh et al. 2014b). The drug-treated cells were exogenously supplemented with different concentrations of cholesterol (50, 100, and 200 mmol/L) and ergosterol (50, 100, and 200 mmol/L). MTT assay was performed to estimate the viability of L. donovani promastigotes upon rescue by cholesterol and ergosterol. All the assays were performed in triplicates, and solvent alone and solvent plus ergosterol were used as controls.
Effect of mevastatin on LdHMGR-overexpressing promastigotes
To determine the specificity of LdHMGR towards mevastatin, we had evaluated its effect on HMGR-overexpressing transgenic parasites which were generated by transfecting the pspα hygroα-LdHMGR construct into promastigotes as reported previously (Singh et al. 2014). Wild-type promastigotes were taken as control. Effect of mevastatin on the growth of wild-type and LdHMGR overexpressors was evaluated by MTT assay.
Alterations in parasite morphology by scanning electron microscopy
L. donovani untreated and mevastatin-treated promastigotes (IC50 and IC90) were harvested and washed twice in ice-cold PBS followed by 4 % (w/v) paraformaldehyde in PBS. The cells were fixed in 4 % (w/v) paraformaldehyde containing 2.5 % glutaraldehyde (Sigma) in PBS and allowed to adhere to poly-l-lysine-coated coverslips at room temperature for 12 h. Samples were dehydrated for 10 min at each step of an ascending ethanol series, mounted on metallic stub by a bioadhesive carbon tape, and sputtered with a thin gold layer. Finally, morphological changes in the parasites were visualized by scanning electron microscope (S-3400N, Hitachi, Japan).
Accumulation of lipid bodies by fluorescence microscopy
Approximately 2 × 106 L. donovani promastigotes were treated with an IC50 (23.3 μM) and IC90 (100 μM) concentration of mevastatin for 48 h. Control and treated parasites were harvested and washed twice in ice-cold PBS. Lipid bodies were labeled with 10 μg/ml of Nile Red (Sigma) and incubated for 30 min at room temperature. Cells were fixed with 4 % paraformaldehyde in PBS and allowed to adhere to poly-l-lysine-coated coverslips. Formation of lipid bodies in the parasites was observed under fluorescent microscope (Leica, Germany) at a magnification of ×100. The images were recorded with a DC300F Leica camera.
Statistical analysis
Statistical analysis of the data was performed using GraphPad Prism software version 5.0. To compare two different groups, one-way analysis of variance (ANOVA) was used. Only values of p ≤ 0.05 were considered as significant.
Results
In vitro activity of mevastatin against L. donovani promastigotes, intracellular amastigotes, and evaluation of mammalian cytotoxicity
Effect of mevastatin on the survival of L. donovani promastigotes was assayed by incubating the parasites with different concentrations of mevastatin (0–100 μM), and percentage viability was calculated by performing MTT assay. Solvent-treated samples were taken as controls in the present study. It was observed that mevastatin induced dose-dependent inhibition of promastigote growth. The IC50 in promastigotes was found to be 23.8 ± 4.2 μM (Fig. 1b). Miltefosine was taken as reference drug. Mevastatin inhibitory effect on intracellular amastigotes was evaluated by transformation rescue assay. The inhibition of amastigotes was also concentration dependent, and the calculated IC50 value was 7.5 ± 1.1 μM (Fig. 1c). The IC50 of miltefosine, standard drug used, was 14 μM against promastigotes and 4 μM against amastigotes, respectively, which correlated well with previously published results (Corral et al. 2014). Cytotoxicity of mevastatin against host cells in vitro was determined using THP-1-differentiated macrophages and showed no inhibitory effect till 100 μM (Fig. 1d). Therefore, the selectivity index for mevastatin was calculated to be >13.3 μM.
Mevastatin inhibits LdHMGR in a competitive manner
To evaluate specific inhibition of mevastatin towards recombinant LdHMGR, the enzyme was purified from E. coli using conditions as mentioned earlier (Dinesh et al. 2014b). Mevastatin caused dose-dependent reduction in enzyme activity with the IC50 value of 42.2 ± 3.0 μM upon treatment with different concentration of mevastatin ranging from 10 to 100 μM (Fig. 2a). To know the inhibitory mechanism of mevastatin, enzyme inhibition studies were performed at different time intervals. The results indicated that the enzyme follows classical Michaelis-Menten kinetics with increasing concentration of HMG-CoA as the substrate (Fig. 2b). The calculated K m and V max were found to be 38.4 ± 6.5 μM and 1.1 ± 0.1 units/mg, respectively, and were similar to the results reported earlier (Dinesh et al. 2014b). At 42 μM mevastatin concentration, K m increased to 81.26 ± 1.7 μM, and it increased further to a K m value of 122.6 ± 9.8 μM at 100 μM of mevastatin. Since the V max of the enzyme remained constant with an increase in apparent K m; it suggested that mevastatin could be acting as a competitive inhibitor of HMGR. The effect of mevastatin (IC50 concentration) on the total cell lysate of promastigotes was also evaluated, and ∼40 % reduction in the HMGR activity in mevastatin-treated promastigote cell lysate compared to untreated cell lysate was observed (Fig. 2c).
Mevastatin alters ergosterol levels in L. donovani promastigotes
To evaluate the effect of mevastatin on ergosterol levels, neutral lipids were isolated from drug-treated and untreated promastigotes and subjected to HPLC. Figure 3a represents standard ergosterol peak obtained at a retention time of ∼11.8 min which correlated with earlier reported data (Dinesh et al. 2014a). Subpanels b and c of Fig. 3, respectively, showed the chromatogram separation of ergosterol isolated from control and mevastatin-treated samples with a peak at a retention time of 11.8 min. Results demonstrated ∼66 % inhibition of ergosterol levels in mevastatin-treated cells compared to the untreated cells (Fig. 3d), thereby demonstrating that mevastatin interferes with the ergosterol biosynthetic pathway.
Ergosterol but not cholesterol reverses mevastatin-mediated growth inhibition of L. donovani promastigotes
To further confirm whether the antiproliferative effect of mevastatin was due to the depletion of ergosterol levels, the promastigotes were treated with mevastatin at its IC50 value for 48 h. Subsequently, the cells were supplemented with ergosterol and cholesterol exogenously to see the restoration of inhibition upon supplementation of sterols by MTT assay. Reversal data revealed that 200 mmol/L of ergosterol causes significant revival of the growth (80 %), whereas the same concentration of cholesterol supplementation had no effect on the revival of the growth (Fig. 3e). Thus, it is clearly evident that mevastatin-mediated Leishmania cell death was only due to the depletion of ergosterol levels. Ergosterol and cholesterol alone did not cause any cytotoxicity to the cells.
HMGR overexpression renders L. donovani promastigotes resistant to mevastatin inhibition
To further prove the specificity of mevastatin towards the HMGR enzyme, effect of mevastatin on the viability of HMGR-overexpressing Leishmania promastigotes was carried out by using HMGR-overexpressing transgenic parasites as reported earlier. The wild-type and HMGR-overexpressing parasites were treated with a range of mevastatin concentration (10–100 μM) and incubated for 48 h. HMGR overexpressors exhibited an IC50 value of 68 ± 4.1 μM, i.e., ∼3-fold higher than the IC50 value of wild-type promastigotes (Fig. 4). Our results confirm that the overexpression of HMGR leads to decreased sensitivity towards mevastatin, and hence HMGR is a specific target for mevastatin.
Mevastatin leads to alteration in parasite morphology
Changes in the morphology of the promastigotes upon mevastatin treatment were investigated using SEM. Figure 5a–c shows the elongated and flagellated shape of the untreated parasites. Subpanels d–f and g–i of Fig. 5 show parasite morphology at an IC50 value of 23.8 μM of mevastatin and IC90 value of 100 μM of mevastatin, respectively. At the IC50 value, the parasite starts losing its elongated shape, and at IC90, it assumes a rounded morphology. This revealed that mevastatin treatment results in alteration in parasite morphology.
Mevastatin causes lipid body accumulation
Cytoplasmic lipid body accumulation upon mevastatin treatment was evaluated by Nile Red staining, which binds to neutral lipids. The fluorescence images demonstrated an accumulation of lipid bodies that are randomly distributed throughout the cytoplasm. As shown in Fig. 6c–f, there was a gradual increase in lipid body formation in parasites with the increase in concentration of mevastatin compared to mevastatin-untreated cells (Fig. 6a, b).
Discussion
In the absence of vaccine for prevention of leishmaniasis, the current treatment regimen is completely based on chemotherapy which is far from satisfactory. Therefore, there is an urgent need to search for new compounds with an antileishmanial potential (Gonzale-Coloma et al. 2012; Mishra et al. 2013). This is the first report demonstrating the antileishmanial activity of mevastatin. Mevastatin, also known as compactin or ML236B, belongs to polyketide group of drugs which includes anticancer drugs, cholesterol-lowering drugs, and immunosuppressants to name a few (Harsha et al. 2013). Statins are known inhibitors of HMGR which catalyzes the rate-limiting step of the sterol biosynthetic pathway leading to synthesis of cholesterol and ergosterol in humans and in fungi and Leishmania, respectively (Stancu and Sima 2001). Besides, statins are also reported to inhibit synthesis of farnesyl pyrophosphate, geranylgeranyl pyrophosphate, guanosine triphosphate-binding protein Ras, and Ras-like proteins (Cordle et al. 2005). Statins have gained lot of attention because of its potential to inhibit the growth of bacteria (Catron et al. 2004), yeast (Song et al. 2003), and protozoa (Wong and Davis 2009). Statins are also reported to exert a broad range of antiviral activity against HIV-1 (Amet et al. 2008), poliovirus (Liu et al. 2006), and cytomegalovirus (Potena et al. 2004). Simvastatin has been shown to inhibit the growth of both procyclic and epimastigote forms of trypanosome with an IC50 value of 25–50 μM (Coppens et al. 1995). Recently, we had reported the antileishmanial efficacy of statins such as atorvastatin and simvastatin (Dinesh et al. 2014b).
Mevastatin is reported to possess hexahydronapthalene skeleton substituted with a β-hydroxy-δ-lactone moiety (Endo 1992). Couto et al. reported that mevastatin alters the synthesis of N-linked oligosaccharides in P. falciparum through the inhibition of dolichol synthesis which is required for isoprenoid pathway. Mevastatin was found to inhibit P. falciparum at 120 μM (Couto et al. 1999). It was also reported to exert antibacterial activity against Chlamydia trachomatis which causes urinogenital infections and blindness (Bashmakov et al. 2010). Mevastatin possesses in vitro anti-hepatitis C virus (HCV) activity (Delang et al. 2009). Mevastatin is also reported to abrogate proliferation of ovarian theca interstitial cell by inhibiting cholesterol biosynthesis (Izquierdo et al. 2004). It has also exhibited anticancer properties by inhibiting colon cancer cell (Ukomadu and Dutta 2003). In humans, HMGR is specifically inhibited by family of potent competitive and irreversible inhibitors like simvastatin and lovastatin (Goldstein and Brown 1990). An intrusion with the mevalonate pathway has been already exploited to fight against Leishmania parasite.
In the current study, we have demonstrated the effect of mevastatin on mevalonate pathway and its specific inhibition of the parasite HMGR enzyme. We observed that mevastatin inhibits not only the extracellular parasite stage but also the intracellular parasite stage in low micromolar range. The IC50 value of mevastatin was determined as 23.8 μM which is very close to the IC50 value of 19.4 μM for atorvastatin and is lower when compared to simvastatin (73.2 μM) against Leishmania donovani promastigotes (Dinesh et al. 2014b). Interestingly, cytotoxicity of mevastatin on macrophage cell line suggested that it has no toxic effect up to 48 h which shows it is parasite selective. The parasite rescue/transformation assay is used to determine the effect of mevastatin on intracellular amastigotes. IC50 value was determined as 7.5 μM which suggested that amastigotes, which are important in establishing the infection, exhibited more sensitivity than promastigotes.
Since mevastatin is a known inhibitor of HMGR enzyme, we evaluated its effect on recombinant LdHMGR enzyme. Mevastatin inhibits the activity of HMGR in a dose-dependent manner. The IC50 value was determined as 42.2 μM which was surprisingly higher than the IC50 concentration determined from the cell-based assay. Kinetic analysis revealed that the inhibition of the L. donovani HMGR activity by mevastatin was competitive. The structural similarity between mevastatin and HMG-CoA and the observed competition by these two molecules explained the structure–activity relationship in the inhibition of HMGR (Endo 1992). It was earlier reported that the inhibition of HMGR by mevastatin is competitive with respect to HMG-CoA in case of rat liver enzyme (Endo et al. 1976). Mevastatin-mediated inhibition of yeast HMGR is reported to be competitive with respect to HMG-CoA (Nakamura and Abeles 1985). However, when the effect of mevastatin was tested on the native parasite HMGR enzyme, it caused 70 % inhibition at 20 μM of mevastatin concentration. Ergosterol is reported to be essential for survival of Leishmania and fungi (Kulkarni et al. 2013). To prove that mevastatin interferes with the sterol biosynthetic pathway, we looked at the alteration in the end product of sterol metabolism, i.e., ergosterol levels. We observed that due to mevastatin treatment, the ergosterol levels drastically reduced (66 %) confirming the specificity of mevastatin to sterol biosynthetic pathway of the parasite. To validate further, various concentrations of ergosterol and cholesterol were added exogenously to the mevastatin-treated cells for the revival of growth. Interestingly, ergosterol caused 80 % reversal of mevastatin-mediated growth inhibition, but cholesterol supplementation could not overcome the growth inhibition. Previous reports indicated that mevastatin-induced block in cell cycle progression in fibroblast cells was overcome by supplementation of mevalonate not cholesterol (Endo 1992). Apart from causing inhibition of HMGR, mevastatin also caused change in parasite morphology from elongated to rounded shape. Mevastatin-induced morphological changes were also reported in microglial cells and fibroblast cells (Schmidt et al. 1982). Effect of mevastatin on accumulation of lipid storage bodies was confirmed through Nile Red staining by fluorescence microscopy. The increased deposition of lipid bodies may suggest the interference of drug with the phospholipids and sterol content. Similar effect on lipid deposition in parasites of Leishmania and Trypanosome was earlier reported with various drugs like 22,26-azasterol, S-limonene, and amiodarone (Britta et al. 2014; de Macedo-Silva et al. 2011; Rodrigues et al. 2002).
Our findings suggested that mevastatin specifically inhibits the sterol metabolism of L. donovani by targeting HMGR. Based on the above results, the following sequence of events are unfolded upon mevastatin treatment (i) inhibition of HMGR enzyme, (ii) alteration in ergosterol levels, (iii) lipid body accumulation, and (iv) parasite death. In conclusion, the efficacy of mevastatin as a potential candidate for treatment of leishmaniasis has been explored, and its mode of action has been elucidated. The effect of mevastatin in an experimental animal model of VL will provide significant insights into its anti-VL efficacy.
References
Alvar J, Velez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M, WHO Control Team (2012) Leishmaniasis worldwide and global estimates of its incidence. PLoS One 7(5):e35671
Amet T, Nonaka M, Dewan MZ, Saitoh Y, Qi X, Ichinose S, Yamamoto N, Yamaoka S (2008) Statin-induced inhibition of HIV-1 release from latently infected U1 cells reveals a critical role for protein prenylation in HIV-1 replication. Microbes Infect 10(5):471–480
Amin-Hanjani S, Stagliano NE, Yamada M, Huang PL, Liao JK, Moskowitz MA (2001) Mevastatin, an HMG-CoA reductase inhibitor, reduces stroke damage and upregulates endothelial nitric oxide synthase in mice. Stroke 32(4):980–986
Bashmakov YK, Zigangirova NA, Pashko YP, Kapotina LN, Petyaev IM (2010) Chlamydia trachomatis growth inhibition and restoration of LDL-receptor level in HepG2 cells treated with mevastatin. Comp Hepatol 9(1):3
Britta EA, Scariot DB, Falzirolli H, Ueda-Nakamura T, Silva CC, Filho BP, Borsali R, Nakamura CV (2014) Cell death and ultrastructural alterations in Leishmania amazonensis caused by new compound 4-nitrobenzaldehyde thiosemicarbazone derived from S-limonene. BMC Microbiol 14(1):236
Catron DM, Lange Y, Borensztajn J, Sylvester MD, Jones BD, Haldar K (2004) Salmonella enterica serovar Typhimurium requires nonsterol precursors of the cholesterol biosynthetic pathway for intracellular proliferation. Infect Immun 72(2):1036–1042
Coppens I, Bastin P, Levade T, Courtoy PJ (1995) Activity, pharmacological inhibition and biological regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in Trypanosoma brucei. Mol Biochem Parasitol 69(1):29–40
Cordle A, Koenigsknecht-Talboo J, Wilkinson B, Limpert A, Landreth G (2005) Mechanisms of statin-mediated inhibition of small G-protein function. J Biol Chem 280(40):34202–34209
Corral MJ, Gonzalez-Sanchez E, Cuquerella M, Alunda JM (2014) In vitro synergistic effect of amphotericin B and allicin on Leishmania donovani and L. infantum. Antimicrob Agents Chemother 58(3):1596–1602
Couto AS, Kimura EA, Peres VJ, Uhrig ML, Katzin AM (1999) Active isoprenoid pathway in the intra-erythrocytic stages of Plasmodium falciparum: presence of dolichols of 11 and 12 isoprene units. Biochem J 341(Pt 3):629–637
Croft SL, Olliaro P (2011) Leishmaniasis chemotherapy—challenges and opportunities. Clin Microbiol Infect 17(10):1478–1483
Danesh FR, Anel RL, Zeng L, Lomasney J, Sahai A, Kanwar YS (2003) Immunomodulatory effects of HMG-CoA reductase inhibitors. Arch Immunol Ther Exp 51(3):139–148
de Macedo-Silva ST, de Oliveira Silva TL, Urbina JA, de Souza W, Rodrigues JC (2011) Antiproliferative, ultrastructural, and physiological effects of amiodarone on promastigote and amastigote forms of Leishmania amazonensis. Mol Biol Int 2011:876021
de Souza W, Rodrigues JCF (2009) Sterol biosynthesis pathway as target for anti-trypanosomatid drugs. Interdiscip Perspect Infect Dis 2009:1–19
Delang L, Paeshuyse J, Vliegen I, Leyssen P, Obeid S, Durantel D, Zoulim F, Op de Beeck A, Neyts J (2009) Statins potentiate the in vitro anti-hepatitis C virus activity of selective hepatitis C virus inhibitors and delay or prevent resistance development. Hepatology 50(1):6–16
Dinesh N, Kaur PK, Swamy KK, Singh S (2014a) Mianserin, an antidepressant kills Leishmania donovani by depleting ergosterol levels. Exp Parasitol 144:84–90
Dinesh N, Pallerla DS, Kaur PK, Kishore Babu N, Singh S (2014b) Exploring Leishmania donovani 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) as a potential drug target by biochemical, biophysical and inhibition studies. Microb Pathog 66:14–23
Endo A (1992) The discovery and development of HMG-CoA reductase inhibitors. J Lipid Res 33(11):1569–1582
Endo A, Kuroda M, Tanzawa K (1976) Competitive inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase by ML-236A and ML-236B fungal metabolites, having hypocholesterolemic activity. FEBS Lett 72(2):323–326
Goldstein JL, Brown MS (1990) Regulation of the mevalonate pathway. Nature 343(6257):425–430
Gonzale-Coloma A, Reina M, Saenz C, Lacret R, Ruiz-Mesia L, Aran VJ, Sanz J, Martinez-Diaz RA (2012) Antileishmanial, antitrypanosomal, and cytotoxic screening of ethnopharmacologically selected Peruvian plants. Parasitol Res 110(4):1381–1392
Harsha N, Rao SS, Sridevi V, Lakshmi MVVC, Kanthi T (2013) Optimization of physicochemical and nutritional parameters for the production of mevastatin using Pencillium citrinum MTCC 1256. IOSR J Pharm 3(1):40–45
Hurtado-Guerrrero R, Pena-Diaz J, Montalvetti A, Ruiz-Perez LM, Gonzalez-Pacanowska D (2002) Kinetic properties and inhibition of Trypanosoma cruzi 3-hydroxy-3-methylglutaryl CoA reductase. FEBS Lett 510(3):141–144
Izquierdo D, Foyouzi N, Kwintkiewicz J, Duleba AJ (2004) Mevastatin inhibits ovarian theca-interstitial cell proliferation and steroidogenesis. Fertil Steril 3:1193–1197
Jain SK, Sahu R, Walker LA, Tekwani BL (2012) A parasite rescue and transformation assay for antileishmanial screening against intracellular Leishmania donovani amastigotes in THP1 human acute monocytic leukemia cell line. J Vis Exp 70:e4054
Jimenez-Jimenez C, Carrero-Lerida J, Sealey-Cardona M, Ruiz Perez LM, Urbina JA, Gonzalez Pacanowska D (2008) Delta24(25)-sterol methenyltransferase: intracellular localization and azasterol sensitivity in Leishmania major promastigotes overexpressing the enzyme. Mol Biochem Parasitol 160(1):52–59
Korlipara K (2002) Statin therapy: rationale for a new agent, rosuvastatin. Int J Clin Pract 56(5):379–387
Kulkarni MM, Reddy N, Gude T, McGwire BS (2013) Voricanozole suppresses the growth of Leishmania species in vitro. Parasitol Res 112(5):2095–2099
Kwintkiewicz J, Foyouzi N, Piotrowski P, Rzepczynska I, Duleba AJ (2006) Mevastatin inhibits proliferation of rat ovarian theca-interstitial cells by blocking the mitogen-activated protein kinase pathway. Fertil Steril 86(4 Suppl):1053–1058
Liao JK (2005) Clinical implications for statin pleiotropy. Curr Opin Lipidol 16(6):624–629
Liu S, Rodriguez AV, Tosteson MT (2006) Role of simvastatin and methyl-β-cyclodextin on inhibition of poliovirus infection. Biochem Biophys Res Commun 347(1):51–59
Mishra J, Madhubala R, Singh S (2013) Visceral and post-Kala-Azar dermal leishmaniasis isolates show significant difference in their in vitro drug susceptibility pattern. Parasitol Res 112(3):1001–1009
Montalvetti A, Pena-Diaz J, Hurtado R, Ruiz-Perez LM, Gonzalez-Pacanowska D (2000) Characterization and regulation of Leishmania major 3-hydroxy-3-methylglutaryl-CoA reductase. Biochem J 349(Pt 1):27–34
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63
Murray HW, Berman JD, Davies CR, Saravia NG (2005) Advances in leishmaniasis. Lancet 366(9496):1561–1577
Nakamura CE, Abeles RH (1985) Mode of interaction of beta-hydroxy-beta-methylglutaryl coenzyme A reductase with strong binding inhibitors: compactin and related compounds. Biochemistry 24(6):1364–1376
Ng HE, Raj SS, Wong SH, Tey D, Tan HM (2008) Estimation of fungal growth using the ergosterol assay: a rapid tool in assessing the microbiological status of grains and feeds. Lett Appl Microbiol 46(1):113–118
Potena L, Frascaroli G, Grigioni F, Lazzarotto T, Magnani G, Tomasi L, Coccolo F, Gabrielli L, Magelli C, Landini MP, Branzi A (2004) Hydroxymethyl-glutaryl coenzyme a reductase inhibition limits cytomegalovirus infection in human endothelial cells. Circulation 109(4):532–536
Pradines B, Torrentino-Madamet M, Fontaine A, Henry M, Baret E, Mosnier J, Briolant S, Fusai T, Rogier C (2007) Atorvastatin is 10-fold more active in vitro than other statins against Plasmodium falciparum. Antimicrob Agents Chemother 51(7):2654–2655
Roberts CW, McLeod R, Rice DW, Ginger M, Chance ML, Goad LJ (2003) Fatty acid and sterol metabolism: potential antimicrobial targets in apicomplexan and trypanosomatid parasitic protozoa. Mol Biochem Parasitol 126(2):129–142
Rodrigues JC, Attias M, Rodriguez C, Urbina JA, Souza W (2002) Ultrastructural and biochemical alterations induced by 22,26-azasterol, a delta(24(25))-sterol methyltransferase inhibitor, on promastigote and amastigote forms of Leishmania amazonensis. Antimicrob Agents Chemother 46(2):487–499
Schmidt RA, Glomset JA, Wight TN, Habenicht AJ, Ross R (1982) A study of the influence of mevalonic acid and its metabolites on the morphology of Swiss 3T3 cells. J Cell Biol 95(1):144–153
Shipman CM, Croucher PI, Russell RG, Helfrich MH, Rogers MJ (1998) The bisphosphonate incadronate (YM175) causes apoptosis of human myeloma cells in vitro by inhibiting the mevalonate pathway. Cancer Res 58(23):5294–5297
Singh S, Dinesh N, Kaur PK, Shamiulla B (2014) Ketanserin, an antidepressant, exerts its antileishmanial action via inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) enzyme of Leishmania donovani. Parasitol Res 113(6):2161–2168
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150(1):76–85
Song JL, Lyons CN, Holleman S, Oliver BG, White TC (2003) Antifungal activity of fluconazole in combination with lovastatin and their effects on gene expression in the ergosterol and prenylation pathways in Candida albicans. Med Mycol 41(5):417–425
Stancu C, Sima A (2001) Statins: mechanism of action and effects. J Cell Mol Med 5(4):378–387
Taylor F, Huffman MD, Macedo AF, Moore TH, Burke M, Davey Smith G, Ward K, Ebrahim S (2013) Statins for the primary prevention of cardiovascular disease. Cochrane Database Syst Rev 1:CD004816
Teo KK, Burton JR (2002) Who should receive HMG CoA reductase inhibitors? Drugs 62(12):1707–1715
Ukomadu C, Dutta A (2003) p21-dependent inhibition of colon cancer cell growth by mevastatin is independent of inhibition of G1 cyclin-dependent kinases. J Biol Chem 278(44):43586–43594
Urbina JA, Lazardi K, Marchan E, Visbal G, Aguirre T, Piras MM, Piras R, Maldonado RA, Payares G, de Souza W (1993) Mevinolin (lovastatin) potentiates the antiproliferative effects of ketoconazole and terbinafine against Trypanosoma (Schizotrypanum) cruzi: in vitro and in vivo studies. Antimicrob Agents Chemother 37(3):580–591
Wong RPM, Davis TME (2009) Statins as potential antimalarial drugs: low relative potency and lack of synergy with conventional antimalarial drugs. Antimicrob Agents Chemother 53(5):2212–2214
Yokoyama K, Trobridge P, Buckner FS, Scholten J, Stuart KD, Van Voorhis WC, Gelb MH (1998) The effects of protein farnesyltransferase inhibitors on trypanosomatids: inhibition of protein farnesylation and cell growth. Mol Biochem Parasitol 94(1):87–97
Acknowledgments
We thank Prof. S. S Sharma, NIPER, S.A.S. Nagar for allowing us to use the fluorescence microscope and Mr. Rahul Mahajan, NIPER, S.A.S. Nagar for handling the scanning electron microscope. Special thanks to Mr. Neerupudi Kishore Babu for critically reading of the manuscript.
Author contributions
SS conceived and designed the experiments, contributed the reagents, and prepared the manuscript. ND and NS performed the experiments and prepared the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Dinesh, N., Soumya, N. & Singh, S. Antileishmanial effect of mevastatin is due to interference with sterol metabolism. Parasitol Res 114, 3873–3883 (2015). https://doi.org/10.1007/s00436-015-4618-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-015-4618-5