Abstract
Torpor is used in small sized birds and mammals as an energy conservation trait. Considerable effort has been put towards elucidating the mechanisms underlying its entry and maintenance, but little attention has been paid regarding the exit. Firstly, we demonstrate that the arousal phase has a stereotyped dynamic: there is a sharp increase in metabolic rate followed by an increase in body temperature and, then, a damped oscillation in body temperature and metabolism. Moreover, the metabolic peak is around two-fold greater than the corresponding euthermic resting metabolic rate. We then hypothesized that either time or energy could be crucial variables to this event and constructed a model from a collection of first principles of physiology, control engineering and thermodynamics. From the model, we show that the stereotyped pattern of the arousal is a solution to save both time and energy. We extended the analysis to the scaling of the use of torpor by endotherms and show that variables related to the control system of body temperature emerge as relevant to the arousal dynamics. In this sense, the stereotyped dynamics of the arousal phase necessitates a certain profile of these variables which is not maintained as body size increases.
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Introduction
To keep a high body temperature under adverse environmental conditions is energetically costly to endotherms in general and particularly for the small-sized ones. For these, the alternative to undergo torpor is a beneficial option to promote survival (Wang and Wolowyk 1988; Heldmaier et al. 2004; Dausmann and Warnecke 2016; Staples 2016; Geiser et al. 2017). Torpor encompasses, albeit in a somewhat confusing nomenclature (Cheng 2008; Mohr et al. 2020), hibernation and daily torpor, and the main differences between them are a more intense metabolic downregulation and longer time-frame in the former, as well as the seasonality of the former. Therefore, birds and mammals which undergo torpor abandon euthermia and downregulate their metabolic rate to reach lower values accompanied by a fall in body temperature (Heldmaier and Ruf 1992; Nogueira de Sá and Chaui-Berlinck 2022) whilst maintaining a steady state condition which may last for several hours, in the case of daily torpor bouts, or for days, weeks and even months, in the case of hibernators.
Descriptive features of the arousal
From a general perspective, during the entry into a torpor bout, body temperature and metabolic rate drop until they reach a new steady-state and, during arousal, they start to change again, reaching back values close to the previous euthermic condition (e.g., Heldmaier et al. 2004; Geiser 2004; Mohr et al. 2020).
Although considerable effort has been put towards elucidating the possible mechanisms underlying entry into torpor and its maintenance (Geiser 2004; Brown et al. 2007; Swoap 2008; Ruf and Geiser 2015; Staples 2016), little attention has been paid regarding the possible mechanisms which may underlie its exit. Such an exit seems to obey a somewhat stereotyped dynamics, as described by several authors. For instance:
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“During an arousal, oxygen uptake showed an exponential increase to its peak, followed by a slight decline to a stable plateau … This pattern of oxygen consumption and activity corresponds with that found by Kayser (1965) who showed that the plateau in oxygen consumption corresponded with rectal temperature of 37 °C … Similarly, Wang (1978) showed that maximal heat loss (and presumably body temperature) in arousing S. richardsonii was not attained slightly after oxygen consumption plateaued … This phase may last from < 1 h in the bat, Eptesicus fuscus (Hayward and Ball, 1966) to ca. 2 h in the ground squirrel …” (Thomas et al. 1990)
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“Arousal was characterized by a metabolic rate overshoot and a rise of body temperature, followed by postarousal with rest metabolic rate and normothermic body temperature of ± 35 °C, usually lasting for only a few hours …” (Song et al. 1997)
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“The shape of this arousal curve, with an initial exponential increase in metabolism, an overshoot, and then an exponential decrease to normal resting levels, is typical of that seen during arousal from torpor in other mammal species.” (Webb and Ellison 1998)
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“Interestingly, one parameter was unchanged by ambient temperature, the temperature gain index. For all ambient temperatures, squirrels reached maximal rewarming rates when they had generated 30–40% of the heat required to reach a euthermic body temperature.” (Utz et al. 2007)
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“However, when the time to rewarm from a body temperature of 5–30 °C was compared, there was no difference among ambient temperature groups”. (Karpovich et al. 2009)
Very similar descriptions of metabolic rate and body temperature profiles during the arousal phase can be found in many other references (e.g., Song et al. 1995; Heldmaier et al. 2004; Sprenger and Milsom 2022), and thus we go to the next step in the analysis.
Quantitative features of the arousal
To migrate from these qualitative descriptions to gain a more quantitative overview of the exit from the torpid state, we examined published graphical data and results encompassing the arousal phase. From these procedures, Table 1 and Fig. 1 were generated. The following relationships were then found.
As expected, due to the extensive nature of energy and the more pronounced drops in body temperature during hibernation (mean Tb = 9.2 °C), there is a significant relation between body mass and time to metabolic peak in hibernation (scaling exponent = 0.29 (0.10–0.47), p-value = 0.0008, r2 = 0.71). This scaling rule results in about \({t}_{\mathrm{peak}}=25\cdot {M}_{b}^{0.29}\) minutes in the mass range from 5 to 800 g to attain metabolic peak, which represents a time frame from 50 to 180 min. On the other hand, daily torpor did not show such a significant relationship between time to metabolic peak and body mass (scaling exponent = 0.05, p-value = 0.77, r2 = 0.006), and the mean time to peak is 78.5 min. In daily torpor, mean Tb = 18.2 °C. These results confirm the textual descriptions of the arousal time presented before and one can assume the exit phase to take something between 1 and 2 h (Fig. 1A).
Next, we examined the association between the time to metabolic peak and the time to rewarm (i.e., the time to attain body temperature near the previous euthermic levels). Clearly, unless for some plots where the time scale did not allow for sharp distinction between the metabolic and the thermic events, the time to the metabolic peak precedes the time to complete rewarming. However, there is no correlation between these times, i.e., the time difference Δt is somewhat fixed: non-significant regression between Δt vs time to peak (slope = − 0.08, p-value = 0.59, r2 = 0.012, see Fig. 1B). The mean value from the metabolic peak to the rewarming time is 23 min. Once again, this reassures the textual description of the arousal presented earlier.
Finally, we considered the value of the metabolic peak and two important points emerge from the analysis. First, there is a ratio between the metabolic peak and the euthermic resting metabolic rate. Second, this ratio does not depend either on the mass of the animal (non-significant regression with slope = 0.13, p-value = 0.54, r2 = 0.017) or on the status (hibernation or daily torpor, p-value for t-test between the groups = 0.74). The ratio has a mean value of 2.09 ± 0.20. Therefore, not only these results confirm the description of a metabolic peak in the arousal phase but they also indicate that a ratio of Mpeak ≅ 2⋅ME composes this metabolic overshoot.
The stereotyped pattern of arousal from torpor
In short, the exit has a somewhat stereotyped dynamic characterized by a sudden increase in both metabolic rate and body temperature followed by several drops and rises until a euthermic steady-state is reached. The peak in metabolic rate is 2 times higher than the euthermic resting metabolic rate. Furthermore, the exit from torpor is a considerably faster process than the entry—while the full torpid state will be attained many hours after the onset of the metabolic downregulation, arousal takes about 1–2 h to reach a metabolic peak and, then, to go to values close to the euthermic body temperature (see, for instance, a detailed description of the arousal phase in terms of body temperature, oxygen consumption, carbon dioxide production and ventilatory rates, times and timing among them in (Sprenger and Milsom 2022)).
From these preliminaries analyses, we construct idealized curves representing body temperature and metabolic rate during the exit phase from the torpid state. This is illustrated in Fig. 2.
At this point, it is important to keep in mind that the increase in metabolic rate, i.e., the metabolic peak, to a value of 2⋅ME implies that the body temperature setpoint must present an initial period of upregulation that leads to very fast changes in the reference temperature, a topic to be dealt later on (see orange control loop in Fig. 3 lower panel). Otherwise, the dynamic is restricted to remain close to euthermic resting metabolic rate and body temperature, without reaching the 2⋅ME peak detected in this previous analysis.
Hypothesis and objective of the present study
Since torpor is considered an energy conserving mechanism, it is possible that events of the exit phase can be elucidated by the study of its metabolic cost. Also, considering the ubiquity of the duration of the arousal, time spent seems to be another weighting factor in the phenomenon. Thus, because of the stereotyped nature of the dynamic observed, we hypothesized that either time or energy could be crucial variables to this phase, which appears to be regulated to allow a secure return to euthermia.
The energy savings of the torpid state were addressed long ago even taking into account the arousal phase (Wang 1979). However, a quantitative model that allows to test whether time or energy is the relevant variable at stake during this phase is not found in the literature. Then, considering the oscillatory features of the arousal phase, the peak in metabolic rate and the importance of time and/or energy consumption to the organism, we constructed a model based on thermal and physiological principles that matches the currently available data to test our hypothesis. The model allows us to explain the relevance of the stereotyped dynamics in terms of optimization (in this case, minimization) of the process based on two criteria: time and energy spent.
Methods
List of symbols
c | Thermal capacitance of body fluids (= 4.12 J g–1 K–1) | T0 | Initial body temperature at the beginning of the arousal phase |
m | Body mass | TS | Operational reference body temperature |
h | Thermal conductance | k | Metabolic rate linear gain |
TB | Body temperature | α | Constant = \({h}^{2}/4\cdot m\cdot c\) |
TE | Euthermic body temperature | r | Non-dimensional gain = \(k/\alpha\) |
TA | Ambient temperature | γ | \(\frac{h}{2\cdot m\cdot c}\) |
M | Metabolic rate | Ω | \(\frac{\alpha }{m\cdot c}\cdot (r-1)\) |
ME | Euthermic resting metabolic rate | g | Linear gain for the setpoint temperature control system |
Q | Power output (heat loss) |
Modelling
A living organism is a control volume, i.e., it exchanges energy and mass with its surroundings (e.g., (Bejan 2002)). In mammals and birds, metabolic rate is the main power input to this control volume. Specifically, during exit from torpor, the system is considered to exchange only heat with the environment which, then, functions as the power output. Also, any mass exchange, e.g., through breathing, is considered irrelevant to metabolism and temperature changes which take place during the process. Any mechanical activity performed by the animal is absent or minimal due to the very nature of torpor. The temperature of the organism (TB) is affected by the power balance between its metabolic rate (M) and heat loss (Q). The scheme in Fig. 3 summarizes the processes described above.
Basics
Metabolic rate contributes to an increase in temperature due to an increase in internal energy, while heat loss gives rise to a decrease. So, the net change in body temperature (in degrees Celsius, °C) is given by the power balance and such a balance that determines body temperature (TB) change can be quantitatively represented as Eq. 1 (Chaui-Berlinck et al. 2005; Sunagawa and Takahashi 2016)—see Fig. 3 lower panel:
In which t, m and c are, respectively, time (in seconds), body mass (in kilograms, kg) and specific heat capacity of the organism (in joules per °C per kg); and, for the sake of notation, it is tacitly assumed that both M and Q are energy fluxes (in watts). M is a positive quantity as long as metabolic activity occurs. For an endotherm, the sign of Q is rarely positive (heat gain). TB is the body temperature.
It was shown that heat loss is linearly proportional to the difference between body and ambient temperatures [for instance (Snapp and Heller 1981)]. Therefore, through Newton’s law of cooling, the heat exchange with the environment is approximated as:
where h is the thermal conductance (in watts per °C) and TA is the ambient temperature.
During the entire process of exit from torpor, TA is considered to be a constant. Total thermal conductance is considered to follow a scaling relationship with mass (Calder 1987; Schleucher and Withers 2001):
A combination of 1 and 2 gives rise to a differential equation:
Rearranging:
where the time dependence of TB and M is yet to be determined.
Temperature regulation
The effect of metabolic rate on body temperature is evident by the fact that the main strategy utilized by endotherms to regulate their body temperature is by adjusting metabolic rate (Nakamura and Morrison 2008; Morrison 2018). Experimental data indicate that body temperature is regulated through a linear control of metabolic rate (e.g., Boulant 2006; Cabanac 2006; Romanovsky 2018), and see (Heller and Colliver 1974; Heller et al. 1974; Chaui-Berlinck et al. 2002, 2005) for specific modelling):
where TS is the operational reference body temperature for the system. There is a debate whether such a term should be setpoint, reference, or some other designation (e.g., Cabanac 2006; Romanovsky 2018)). It is not within the scope of this study to participate in this debate; thus, it will not be addressed. Even more important than do not take part in this debate are the facts that from the standpoint of control whether a reference value is a pre-set in the system or a construct of its interacting parts is not relevant and that a reference value can be changed at will.
Therefore, the terms setpoint and reference will be used interchangeably only to indicate to which temperature the system is lead to by the controller (Sunagawa and Takahashi 2016). The parameter k is the linear gain of the control system (in watts per °C per second)—it represents the intensity of change in metabolic rate given a certain deviation in body temperature from the setpoint TS.
Treating the torpor condition as a steady state (dM/dt and dTB/dt equal to zero), both body temperature and the operational reference are equal and at a fixed value T0 (the initial body temperature). For the organism to exit from torpor and reach euthermia, the setpoint should increase to euthermic values, such that metabolic rate starts to increase according to Eq. 6. The following equation describes changes in TS at the onset of exit from torpor:
In which TE is euthermic body temperature and g is the linear gain for the setpoint temperature control system (in seconds–1) occurring once the organism prepares to leave the torpor condition. In other words, instead of a non-linear discontinuous step change in the temperature setpoint at a given time, Eq. 7 represents a continuous change leaving the torpid setpoint towards the euthermic one, and the steepness of the change is given by the linear gain g. Considering the initial condition TS(t = 0) = T0, the solution to this first order differential equation is given by:
The constant g is the inverse of the characteristic time of TS. The characteristic time of changes in body temperature is given by the factor mc/h (see Eq. 5). If we assume g is higher than the possible values for k, then TS reaches the euthermic value TE faster than dM/dt reaches zero (see Eq. 6). In other words, the change in TS from T0 to TE marks the beginning of the process of metabolic rate increase. Therefore:
Taking into consideration this explicit equation for TS, regulation of body temperature is represented by:
Linear control system
Next, we consider the influence of both the power balance on body temperature (M − Q, see Eq. 1), and its regulation through the linear control described above (see Eq. 6). Mass and thermal conductance are individual-dependent parameters and were kept constant to study the effects of the linear gain k. We can identify a constant term, \(\alpha ={h}^{2}/4\cdot m\cdot c\), and also define a non-dimensional variable \(r=k/\alpha\) (see below). This non-dimensional variable \(r\) is directly proportional to the linear gain of the metabolic rate (Eq. 6), or, in other words, it can be interpreted as the gain in the system.
Equations 5 and 10 form an ordinary, first order, non-homogeneous system of differential equations. Through manipulation of Eq. 5 it is possible to express M in terms of TB and its derivative:
Differentiation leads to:
Equation 12 can be inserted into Eq. 10:
And rearranged:
where TS was reinserted. The solution of Eq. 14 can be split in two: the general solution to the homogeneous equation and the particular solution to the non-homogeneous one. The homogeneous solution is given by the characteristic equation:
The solution of the quadratic Eq. 15 is:
We then introduce the following simplifying combinations of factors:
After inserting Eqs. 17 into Eq. 16, the solution becomes:
The value of Ω and, thus, of r, is important to determine the behaviour of the system. The three possible solutions are Ω < 0, Ω = 0 and Ω > 0, which translate to r < 1, r = 1 and r > 1, respectively. This defines three bands of the metabolic rate control system: \(k<{h}^{2}/4\cdot m\cdot c\), \(k={h}^{2}/4\cdot m\cdot c\) and \(k>{h}^{2}/4\cdot m\cdot c\). These three bands are classically referred to as overdamped, critically damped and underdamped systems, respectively, due to the similarities to a spring–mass system. Since k is the linear gain of body temperature regulation, the solutions will be referred to match the properties of the control system: low, critical, and high gain. These three solutions will be identified by their respective values of r, a non-dimensional value that encompasses the relationship between the gain k and \({h}^{2}/4\cdot m\cdot c\). Their functional forms for the homogeneous solutions (hom) are as follows:
The constants C1 and C2 are to be determined by applying initial conditions to the system of Eqs. 5 and 10 and are different for each case. A particular solution to the non-homogeneous Eq. 14 is given by TB,part = TS, where the subscript part was used to specify the particular solution. This results in the unique general solution of Eq. 14 (according to Picard’s uniqueness and existence theorem):
The dynamic for metabolic rate is given by reintroduction of TB into Eq. 11:
By recognizing that, at euthermia, the organism should have constant body temperature TE and applying the euthermic condition to Eq. 4, euthermic resting metabolic rate ME can be defined:
The application of initial conditions to the three pairs of Eqs. 22/25, 23/26 and 24/27 defines the values of the constants C1 and C2 for each case. As described before, initial body temperature is given by T0 and initial body temperature change, dTB/dt, is zero. This is equivalent to affirm that initial metabolic rate, M(t = 0), should be given by h(T0–TA) = M0. The subsequent derivation of C1 and C2, together with Eq. 28, results in the three complete solutions of the system.For r < 1:
For r = 1:
For r > 1:
The dynamics for r > 1 behave similarly to the experimental data currently available (see Figs. 1, 4 and 5), where both the damped oscillations and a distinct peak of metabolic rate are observed.
Results
General dynamics
Figure 4 shows the time course given by Eqs. 33 and 34 for different values of r > 1 (see caption). The numerical values of the parameters body mass, specific heat capacity, thermal conductance, euthermic body temperature and ambient temperature are specified in the Appendix—Upregulation of Ts.
At this point, we have the mathematical framework for the exit from torpor. However, the 2·ME peak is not yet achieved. Therefore, as anticipated, there should be a period of fast changes in TS towards the euthermic value. This can be accomplished by an upregulation of TS (see Appendix and Fig. 3 lower panel in orange). In fact, this resembles a mirror image of the entry phase where Sunagawa & Takahashi have shown a reduction in the sensitivity (i.e., gain) of the thermo-control system (Sunagawa and Takahashi 2016).
Given the timeframe of exit from torpor, a mean value of 1.5 h was chosen as the period of upregulation, in which the aimed value is 45 °C. This is also consistent with the 60 min time-frame of changes in hypercapnic ventilatory response observed in the arousal from hibernation in 13-lined ground squirrels (Ictidomys tridecemlineatus—(Sprenger and Milsom 2022)).
The time course of Eqs. 33 and 34 with the upregulation in TS can be seen in Fig. 5 for different values of r. The metabolic peak of approximately 2·ME is now obtained. It is important to note that, even though the aimed value for the setpoint is 45 °C, the actual body temperature is kept within the physiological range.
What is left to tackle in this study is a relationship between these different values of r and the hypothesized optimization of either time and/or energy spent. This is shown in Fig. 6, that contain the total time and energy spent during exit from torpor—up to the point when Tb reaches TE for the first time—for different r values.
The minimum in each plot was determined to be at r = 72.5 for time and r = 58 for energy spent. The minimal values are shown in Table 2, together with an intermediate value of r, 65.5. As it can be clearly observed, there is little to no variation in time and energy spent within this range of r. In other words, the exact value of r is irrelevant to the minimization of both these quantities, as long as the control system of the organism remains close to these high r values. The choice of 65.5, although arbitrary, illustrates this. Figure 7 shows how metabolic rate and body temperature behave according to this value. Both the total energy and total time spent are close to the minimum and the 2·ME peak is achieved, giving rise to a dynamic quite similar to the one presented in Fig. 1.
Scaling
A broader application of our model was tested to understand how body mass affects the minimal values of time and energy spent, and also the values of r that satisfy these minima. Figures 8 and 9 show how these relations hold. Minimal time spent scales with an exponent of 0.55, while minimal energy, with 1.03. In other words, time scales approximately with the square root of body mass and energy is linear with body mass, with an inclination of 0.25 kJ/g. On the other hand, r values do not show a simple scaling rule, and, in fact, have an optimum value of around 65 for body mass below 100 g, and around 25 for body mass above 100 g (see Fig. 9). It is important to keep in mind that there are no confirmed examples of true torpor in large mammals (e.g., (Heldmaier et al. 2004)), and, therefore, the scaling of the thermal conductance is more adequate for small animals.
Comparing the model to real data of daily torpor
The model was applied to a mammal of 25 g (Sminthopsis macroura) exiting daily torpor (Geiser et al. 1996). The parameters of the simulation were TA = 19, TE = 34 and T0 = 21, as in the original experiment. Figure 10 shows the comparison between real data and the model—notice the striking resemblance between them.
Discussion
Initially, it is important to note that the aim of the present study was neither to address any specific case nor to make any model fitting to particular events. Our goal was to consider a general problem—the somewhat stereotyped dynamics of the arousal from the torpid state—and use minimal information to address the issue of whether this stereotyped dynamic optimize the transition to the euthermic condition. We hypothesized that minimization in time or in energy consumption might underlie this metabolic behaviour and addressed the issue building a mathematical framework.
The model was constructed from a collection of first principles of physiology, control engineering and thermodynamics, namely: the power balance between metabolic rate and heat exchange, and endothermic body temperature control system. Moreover, the model is quite parsimonious: it is based on only three coupled linear differential equations (eqs. 1, 6 and 7). When we applied these principles to the torpor arousal phase, the model produced patterns in both body temperature and metabolic rate dynamics that reproduce real data accurately (compare Figs. 1 and 7 and see Fig. 10).
During torpor, an animal is subjected to physiological conditions that might jeopardize survival, as the low macromolecular turnover and cellular repairing rates (Storey 2010), thermal stress associated to energy depletion or other oxidative stressors (Carey et al. 1999, 2000). In fact, the arousal phase, due to the increased oxygen demand, might impose risks for its own (Lee et al. 2002; Storey 2010). In this sense, it is ample documented the use of the so-called cold arousals in bats during hibernation (Bartonička et al. 2017; Mayberry et al. 2018). These cold arousals are slight elevations in body temperature without completing the return to euthermia and seem to fulfil a yet unknown role. Besides cold arousals, hibernating animals might present the so-called alarm arousals, which are a complete return to euthermic conditions due to external perturbations (Utz and van Breukelen 2013) and follow a similar time pattern as the natural ones. Therefore, the full arousal is also expected to preserve the integrity of the organism in the best possible way.
Given that torpor is thought to be an energy-preserving mechanism, it makes sense that the energy spent should be minimized during the whole process, including its exit. Moreover, before they get access to food after arousal, most of the hibernators and daily torpids shall become active (Hume et al. 2002; Cotton and Harlow 2010; Tøien et al. 2011; Gao et al. 2012; Hindle et al. 2015) and, thus, need their energy storages to not be completely exhausted (Dausmann and Warnecke 2016; Bartsiokas and Arsuaga 2020).
The model shows that the stereotyped pattern of exit from torpor is in fact a double solution: it saves time and preserves energy storages. In other words, even though our primary hypotheses were a minimization in either time or energy, we found that it is possible for the controller to simultaneously minimize both (see Table 2).
Figure 6 shows that there is a given range of r, approximately between 58 and 72.5, for which there is little to no variation in both the time and the energy spent during exit from torpor. This parameter r is a measure of the intensity of the linear gain of the control system of body temperature. Therefore, the higher the value of r, the greater the acceleration of metabolic rate responses. The opposite is true for low values of r, which imply a slow response in metabolism.
The fall in body temperature during the entry phase is accounted for by a decrease in the hypothalamic setpoint (Heller and Kilduff 1985) and changes related to the autonomic nervous system (i.e., heart and respiratory rates, cerebral blood flow and oxygen consumption) lead changes in body temperature (Drew et al. 2007). The rise in body temperature and metabolic rate during exit from torpor is credited to a rise in the hypothalamic setpoint (Sunagawa and Takahashi 2016). Therefore, similar to the entrance phase, changes in metabolic rate precede changes in body temperature. The current view regarding the activation of heat production in the arousal from torpor holds that it is a sympathetically driven process (Braulke and Heldmaier 2010; Nedergaard and Cannon 2018; Shi et al. 2021)). This increase in sympathetic activity might explain both the increase in heat production and the overshoot in the temperature setpoint. In this sense of sympathetic activation, it was observed that systolic blood pressure has an overshoot that precedes body temperature peak (similar to what happens to metabolic rate) during the arousal phase from hibernation in Syrian hamsters (Horwitz et al. 2013), indicating, as in the case of the hypercapnic ventilatory response cited earlier, that the references in the controllers are in change during this phase. The termination in the setpoint overshoot is probably due to the rise in body temperature itself which would close a loop in another control layer. This investigation is left to other studies.
From a slight different perspective, the alarm arousals cited earlier show an interesting point: body temperature increases at a faster rate than in natural arousals (Utz and van Breukelen 2013). This fact, as the authors conclude, might indicate that natural arousals are controlled to occur at a slower pace than the maximum possible for the animal. This observation reinforces our present analysis: the stereotyped time-profile of natural arousals is a controlled process to minimize both time and energy during the exit from torpor.
Thus, we conjecture that the exit from torpor occurs by obeying the following events:
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1.
once the arousal is triggered in the central nervous system, during a brief period of time (e.g., 1 h) the setpoint is overshot to a value above the euthermic temperature;
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2.
this causes a huge acceleration in metabolic rate according to the gain in the control system;
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3.
then, body temperature starts to rise in an accelerated way as well;
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4.
after that brief period of time the setpoint is set to the euthermic value and metabolic rate and body temperature tend to damped oscillations around their respective euthermic values.
The importance of the gain in the control system lies in the amount of time and energy spent during this arousal process. Experimental data evidence that small hibernators mammals have, indeed, a higher gain in their temperature control system than larger non-hibernating ones (Heller et al. 1974), and, also, that such a gain is higher in euthermia than in the torpid state (Sunagawa and Takahashi 2016), as predict by the model.
Our modelling implies that large mammals are restricted in the use of torpor due to the optimal values of the linear gain in temperature regulation, around r = 25. These values, combined with the setpoint parameters τ and TG (see Appendix), do not allow a metabolic peak of 2·ME (simulations not shown). Clearly, there are many factors involved in the restriction of metabolic depression in large endotherms other than the optimality in the arousal phase which are beyond the present modelling.
Large mammals are observed to hibernate with only a small drop in TB and a slow exit profile (Tøien et al. 2011; Choukèr et al. 2019), without the metabolic peak characteristic of torpor. This suggests a metabolic restriction in the arousal, as indicated by our model. In this sense, as proposed by other authors, body temperature should not be the criterion to define hibernation (Dausmann et al. 2005). From the present study, we suggest that this ratio between Mpeak and ME could be employed to distinguish among depressed metabolic states.
Temperature effects on metabolic rate is a highly debatable issue. Many understand it as a causative factor: body temperature itself causes the metabolic rate attained. Some understand it as a permissive factor: the increase in body temperature allows for an increase in chemical reaction rates (Chaui-Berlinck et al. 2004). The former, usually cited as “Q10 effect”, is incompatible with metabolic control of thermoregulation while the latter does not interfere with thermoregulation via metabolic control. The problem is that which view of “temperature effects” the authors are considering is seldom made explicit in the reports.
While there is an equivocal debate regarding temperature effects on metabolic rate, viz “Q10 effects”, in the entry phase (Snyder and Nestler 1990; Heldmaier and Ruf 1992; Heldmaier et al. 1999, 2004; Ortmann and Heldmaier 2000; Nogueira de Sá and Chaui-Berlinck 2022), the arousal was never truly credited to these effects. Utz and collaborators (Utz et al. 2007) made an attempt to input the increase in metabolic rate to temperature effects (Q10 effects) during the exit phase and verified, as the authors state, “the curious fact” that the arousing animals show a decrease in their rates of rewarming around the middle of the process even though body temperature continues to rise, and clearly this cannot be explained through temperature effects on metabolic rate. Our model clarifies this because there are no causative temperature effects on metabolic rate at all, and the “curious fact” highlights the control system at full action, indeed. Clearly, this does not preclude temperature as a permissive effect.
Data availability
All data are available in the main text.
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Appendix
Appendix
Upregulation of T s
The graphical visualization of the dynamics of M and TB, determined by Eqs. 29 through 34, requires that numerical values are assigned to all constants. The organism is considered to have body mass m = 50 g, specific heat capacity c = 4.12 J/oC·gram (Haemmerich et al. 2005), euthermic body temperature TE = 38 °C and initial temperature T0 = 12 °C. Considering Eq. 3 and a mass of 50 g, total thermal conductance (h) has a value of 0.001310 watts/oC (0.22 mL O2 h−1 °C−1). The environment is considered to be at constant temperature TA = 10 °C. These values result in an euthermic resting metabolic rate ME = 1.16 watts and an initial metabolic rate M0 = 0.083 watts, independently of the gain k. For the sake of comparison, the elephant shrew Elephantulus rozeti with body mass of 45 g at TA of 10 °C has an euthermic oxygen consumption rate of 5 mL O2 g−1 h−1 which results in 1.26 watts (Lovegrove et al. 2001).
The time course of metabolic rate and body temperature described by Eqs. 34–39 are plotted in Fig.
11. Note that the brighter lines, which represent the high gain case, have dynamics that resemble the stereotyped behaviour of exit from torpor shown in Fig. 1. Therefore, in the remaining of the analysis, we focus only on values of r greater than 1.
As explained in the main text, we have the mathematical framework for the general behaviour of the exit from torpor, nevertheless, without the 2·ME peak. This can be accomplished by an upregulation of TS. To implement the change in the setpoint during the process of exit, Eq. 7 was changed to:
where TF(t) is given by:
TG is a value which the setpoint would try to achieve before aiming for TE, τ is the time at which the setpoint dynamic would change and \(\theta\)(t) is the Heaviside Step Function. Equation 36 describes a function which has constant value TG until time \(\tau\), after which there is a step change to TF(t > \(\tau\)) = TE. Considering the same 50 g organism described before, we have TG = TE + 7 °C (in this case, 45 °C) and \(\tau\) = 5,400 s (1.5 h). New equations for TS, TB and M were obtained through the following system:
Numerical integrations were done for TS, M and TB—the unique dynamic for TS is shown in Fig.
12, while some dynamics for M and TB, which depend on r, are shown in Fig. 5 of the main text.
Calculations of the total time and energy spent during the process were done for different values of r. Total time was defined as the time at which TB reaches and crosses TE for the first time (after that, it starts to oscillate around TE). The total energy spent was the integral of M(t) from time zero to that total time. Figures 6 and 7 show these results (see main text).
For different values of r, TB will cross TE at different phases of the oscillation, giving rise to the discontinuity seen in Figs. 6 and 7, at around r = 85. The insets in Fig. 5 illustrate this.
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Nogueira-de-Sá, P.G., Bicudo, J.E.P.W. & Chaui-Berlinck, J.G. Energy and time optimization during exit from torpor in vertebrate endotherms. J Comp Physiol B 193, 461–475 (2023). https://doi.org/10.1007/s00360-023-01494-5
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DOI: https://doi.org/10.1007/s00360-023-01494-5