Abstract
The annual routines and seasonal ecology of herbivorous zooplankton species are relatively well known due to their tight coupling with their pulsed food source, the primary production. For higher trophic levels of plankton, these seasonal interactions are less well understood. Here, we study the mid-winter feeding of chaetognaths in high-Arctic fjord ecosystems. Chaetognaths are planktivorous predators which comprise high biomass in high-latitude seas. We investigated the common species Parasagitta elegans around the Svalbard archipelago (78–81°N) during the winters of 2012 and 2013. Our samples consisted of individuals (body lengths 9–55 mm) from three fjords, which were examined for gut contents (n = 903), stable isotopes, fatty acid composition, and maturity status (n = 352). About a quarter of the individuals contained gut contents, mainly lipid droplets and chitinous debris, whilst only 4 % contained identifiable prey, chiefly the copepods Calanus spp. and Metridia longa. The δ15N content of P. elegans, and its average trophic level of 2.9, confirmed its carnivorous position and its fatty acid profile [in particular its high levels of 20:1(n-9) and 22:1(n-11)] confirmed carnivory on Calanus. Observations of undeveloped gonads in many of the larger P. elegans, and the absence of small individuals <10 mm, suggested that reproduction had not started this early in the year. Its average feeding rate across fjords and years was 0.12 prey ind.−1 day−1, which is low compared to estimates of spring and summer feeding in high-latitude environments. Our findings suggest reduced feeding activity during winter and that predation by P. elegans had little impact on the mortality of copepods.
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Introduction
The Arctic is a highly seasonal environment, with conditions in the marine pelagic varying greatly between summer and winter. The extreme cycles in solar illumination and sea ice cover create strong seasonality in primary production (Ji et al. 2013), which affects food availability for organisms at higher trophic levels (Varpe 2012). Food availability for herbivores such as Calanus copepods is severely reduced during the polar night. Many grazers survive winter food shortages by resting at depth and sustaining themselves on storage lipids accumulated when food was last available (e.g. Conover 1988). Although pelagic omnivores and carnivores may experience a less-pulsed food source than for the herbivores, they may also display adaptations to a seasonal food source (e.g. Newbury 1971; Choe et al. 2003; Kraft et al. 2013). However, for many predators we lack knowledge of their feeding opportunities and activities during winter.
The chaetognath Parasagitta elegans (Verrill 1873) is highly represented in Arctic mesozooplankton communities, numerically and in biomass terms (e.g. Søreide et al. 2003; Hopcroft et al. 2005). This typically neritic species (Bieri 1959) can exert high predation pressure on Arctic copepods and compete with larval fish (e.g. Samemoto 1978). Some authors have proposed that chaetognaths are not particularly sensitive to seasonality (e.g. Hagen 1999), suggesting that their varied diets (reviewed by Terazaki 2004) and non-visual food search should allow them to find adequate nutrition throughout the year. Lipid-rich copepods, often preferred prey, diapause in large densities and in an unalert state during winter, so they may be easy prey for some carnivores (Darnis et al. 2012). However, some chaetognath species at high latitudes are found to contain considerable amounts of lipids, including wax esters, which may be used for storage (e.g. Lee 1974; Kruse et al. 2010). Furthermore, winter growth rates of P. elegans in the Arctic is lower than those in spring and summer (Grigor et al. 2014), and these observations could indicate reduced winter feeding and resting strategies in this chaetognath.
We report here on the winter feeding ecology of P. elegans in the European high Arctic. We used three different methods to assess its diet and trophic level: gut content analysis, which records recent feeding, as well as stable isotope and fatty acid trophic marker (FATM) analyses, which provide additional information on its feeding history, over time frames of weeks to months (Graeve et al. 2005). We calculated feeding rates and possible impact on copepod populations. Although we did not expect mid-winter reproduction (e.g. Kramp 1939; Grigor et al. 2014), we also examined their level of maturity, allowing us to infer how close P. elegans is to reproducing, which links to how actively individuals are expected to feed.
Materials and methods
Study area
Chaetognaths were collected from waters around Svalbard (78–81°N) during the winters of 2012 and 2013, during the ARCTOS “Polar Night Cruises” (8 to 21 January 2012 and 9 to 18 January 2013).
The Svalbard archipelago is a meeting place for warm Atlantic water sourced from more southern regions and colder Arctic water from the north. The northward moving West Spitsbergen Current deposits Atlantic water into fjords on the west coast, with one branch finally turning north-east at the top of Spitsbergen and affecting conditions in the Arctic Ocean (Saloranta and Haugen 2001). We sampled three fjords in 2012: Rijpfjorden (station R3), Isfjorden proper, and Adventfjorden, a tributary fjord of Isfjorden (station ISA, see Fig. 1). The latter two locations are hereafter referred to as “Isfjorden”. Another fjord, Kongsfjorden, was sampled in 2013, and Rijpfjorden was re-sampled. See Table S1 for full details on the sampling activities. Isfjorden (78°N, 14°E) and Kongsfjorden (79°N, 11.5°E) are influenced by Atlantic water masses and the outer parts are ice free for much of the year (Svendsen et al. 2002; Nilsen et al. 2008), causing important seasonal changes in biodiversity and animal populations in these fjords. Rijpfjorden, in contrast (80°N, 22°E), is influenced by Arctic water, typically remaining ice-covered from December/February to July (Wallace et al. 2010).
Physical and biological environment
At all stations, vertical profiles of salinity, temperature, density, and fluorescence were obtained using a CTD (SBE 9) and processed following standard Sea Bird Electronics (SBE) procedures. In 2012, a steep thermocline was observed in Rijpfjorden between ~70 and 100 m, over which temperature rose to 2 °C and then fell again to 0.5 °C at ~200 m (Fig. S1 in the Supplement). At the mouth of Isfjorden, the thermocline was much weaker, with temperature increasing gradually to 2 °C at ~200 m. At Isfjorden (station ISA), temperature varied little with depth (Fig. S1). At all stations, salinity varied with depth in similar ways to temperature. A strong halocline occurred in Rijpfjorden only, at the same depths as the thermocline (Fig. S1). Fluorescence was very low (<0.6 g l−1) at all stations in January 2012.
Zooplankton sampling
Horizontal trawls of the large Methot Isaac Kidd gear (MIK, 3.14-m2 opening, 1.5-mm mesh) were used to obtain large numbers of P. elegans for the various dietary analyses (Table S1). MIK sampling depths in Rijpfjorden and Isfjorden (in 2012) captured all water masses in the fjords (melt water, Arctic water/mixed Fram Strait water and Arctic deep water). In contrast, most MIK sampling for chaetognaths in 2013 (Kongsfjorden and Rijpfjorden) was carried out in the upper 20 m (Table S1). This sampling decision was taken to reduce haul time, as chaetognaths can easily become damaged and stressed during net sampling, which poses several problems for gut content analyses (Baier and Purcell 1997).
In Rijpfjorden in 2012, vertical hauls of the smaller Multi-Plankton Sampler gear (MPS, 0.25-m2 opening, 0.2-mm mesh) were also performed to collect zooplankton community data (see also Daase et al. 2014) and to source smaller P. elegans for gut content analyses (sampled strata: 260–200 m, 200–100 m, 100–50 m, 50–20 m, 20–0 m; see Table S1 for details). The MPS captured higher densities of smaller P. elegans (10–19 mm) in the upper 20 m than the MIK (0.5 ± 0.4 compared to 0.1 ± 0.1 ind. m−3), but fewer individuals ≥20 mm (1.8 ± 1.7 compared to 5.9 ± 8.2 ind. m−3). Individuals <10 mm were not captured by either gear in Rijpfjorden or Isfjorden in 2012. Grigor et al. (2014) similarly showed that the MPS captured the smaller size fraction of P. elegans more efficiently than a larger “WP3” net with a 1-m2 opening. MPS samples collected from Isfjorden ISA on 27 January 2012 were also analysed for abundance data.
Sample processing
Upon retrieval of each MIK haul, the cod end was immediately transferred to a marked bucket and diluted up to the 10 l mark. A 0.6 l sub-sample was taken for community analysis and fixed in 4 % buffered formalin–seawater solution. Webster et al. (2013) show presence–absence data for zooplankton in Rijpfjorden from the community samples from 2012. From a second 0.6 l sub-sample, 100 P. elegans were randomly picked out for gut content analyses (Table S1) and preserved in 4 % buffered formalin–seawater solution. On the 2012 cruise, 75–150 P. elegans were also picked out of this second sub-sample for stable isotope analyses (n individuals = 675, Table S1), and 30 individuals were randomly picked out for fatty acid trophic marker (FATM) analyses (n individuals = 290, Table S1). These samples were frozen at −80 °C until the end of the cruise and then stored at −80 °C for further processing.
Gut content analyses
A total of 202 individuals from 2012 (body lengths 10–55 mm) and 701 from 2013 (body lengths 9–42 mm) were dissected and analysed for gut contents. In 2012, a minimum of 10 individuals were dissected from each MIK haul. In 2013, we examined the guts of all 100 individuals preserved from each MIK haul; 3–10 smaller individuals were sourced from each MPS haul (Table S1).
Parasagitta elegans individuals were measured to the nearest millimetre (excluding the caudal fin) and stained with a solution of lignin-red, used to stain tissues of crustacean prey in their guts (Falkenhaug 1991), and borax carmine, capable of highlighting the gonads of prey (Pierce 1941), diluted 50 times in water. Head widths were measured to the nearest 0.01 millimetre under the binocular microscope. All visible gut contents were described. Detected prey was identified to the lowest possible taxonomic level and photographed using a digital camera connected to a dissecting microscope (Leica DFC 320).
Food-containing ratio and feeding rate
The food-containing ratio (FCR) is the proportion of individuals in a population with food in their gut. Some only include chaetognaths containing identifiable prey in the FCR calculation (FCRmin, e.g. Falkenhaug 1991; Kruse et al. 2010). However, this approach may not account for loss of prey items from guts during net towing, due to damage or stress-induced regurgitation (Baier and Purcell 1997, see “Discussion”). Other studies also included individuals with other signs of recent prey digestion (gut lipids and debris, e.g. Samemoto 1978; Froneman and Pakhomov 1998), which may give an upper estimate of the proportion feeding (FCRmax). FCRmax measurements may not be appropriate for some chaetognath species, such as those that contain oil vacuoles in the centre of their bodies. This includes another Arctic species, Eukrohnia hamata (Möbius 1875), in which oil vacuoles may be for storage or buoyancy (Øresland 1990; Pond 2012, pers. obs.). However, these oil vacuoles do not occur in P. elegans. We calculated both FCRmax and FCRmin for P. elegans from each haul. We also calculated feeding rates (FR: no. of prey items consumed ind.−1 day−1) according to Eq. (1). Only individuals containing identifiable prey were included in the FR calculation.
where n prey = mean no. of identifiable prey per chaetognath, and t dig = digestion time in hours. Feigenbaum (1982) suggested a t dig of 10.2 h for P. elegans from a laboratory study on starved specimens from Vineyard Sound, maintained at 0 °C. As the water temperature in all our study fjords was close to 0 °C (Fig. S1), we used Feigenbaum’s t dig estimate.
Proportions of individuals per haul containing different gut types were analysed using Kruskal–Wallis tests.
Stable isotope analyses
As the ratios of 13C/12C and 15N/14N in organisms at different trophic levels tend to differ considerably, stable isotope analyses can be used to examine marine food web structure and the transfer of energy between trophic levels (McConnaughey and McRoy 1979). Stable isotope analyses were performed on our 2012 samples at the Institute for Energy Technology in Kjeller, Norway, using similar methods to Søreide et al. (2006). Three replicate samples each containing 25 P. elegans were analysed from seven of the MIK hauls, whilst six replicate samples of 25 inds. were analysed from an eighth MIK haul at 225m in Rijpfjorden (Table S1). All P. elegans had body lengths within the range of 10–50 mm. The whole body of each chaetognath was used, except lipids and non-dietary carbon (i.e. carbonates), which were removed before analyses by Soxhlet extraction with CH2Cl2. Lipids have notably lower levels of 13C than proteins and carbohydrates (van Dongen et al. 2002), and their removal reduces a main source of measurement variability between individuals (Hobson and Welch 1992). C/N ratios were expressed as the deviation from standards in ppt (‰) according to Eq. (2) (Søreide et al. 2006).
where X = 13C or 15N and R = the corresponding ratios 13C/12C or 15N/14N. International standards, Pee Dee Belemnite for δ13C (PDB: USGS 24), and atmospheric air for δ15N (IAEA-N-1 and 2) were used to determine R. Carbon and nitrogen composition were expressed as percentages of animal dry weight.
Determination of trophic level
The trophic level (TL) of P. elegans in each fjord was calculated as the difference between its average δ15N content and that of the winter food web baseline, assuming a constant fractionation between trophic levels [Eq. (3), Søreide et al. 2006].
where α = trophic level of the food web baseline and ΔN = the trophic enrichment factor for δ15N (average amplification) per trophic level. We took the abundant grazer Calanus glacialis (α = 2) to represent the winter food web baseline. This decision was made because P. elegans are thought to be carnivores that typically do not feed on primary production but feed often on Calanus, and primary production is anyway low in winter (pers. comm.: J Søreide).
We used a δ15N value for Calanus glacialis of 9.6 ± 0.2 ‰, estimated for individuals from 300 m in Svalbard fjords during December (Søreide et al. 2008). We used ΔN = 3.4 ‰, also determined for the European Arctic by Søreide et al. (2006).
Fatty acid analyses
Groups of primary producers and some herbivores produce fatty acids that are unique to them, known as fatty acid trophic markers (FATMs, Dalsgaard et al. 2003). Higher consumers possess a lower capacity to synthesise their own fatty acids, or to modify those received from their prey. Therefore, when FATMs persist in a predator for some time with little modification, they can be used to quantify its diet (Dalsgaard et al. 2003).
Determination of the fatty acid signatures of P. elegans from Isfjorden and Rijpfjorden (in 2012) were performed on triplicate samples from nine MIK hauls (Table S1) at UNILAB, Tromsø, Norway, using similar methods to Wold et al. (2011). Each sample contained 10 pooled individuals with dry weights between 0.001 and 0.05 g. Total lipid was extracted in 15 ml 2:1 chloroform–methanol with butylated hydroxytoluene (BHT) (Folch et al. 1957). Each sample was supplemented with a known amount of the fatty acid 21:0, as an internal standard, and trans-methylated in methanol containing 1 % sulphuric acid with toluene for 16 h at 50 °C. The relative compositions (%) of FA methyl esters were determined on an Agilent 6890 N gas chromatograph, equipped with a fused silica, wall-coated capillary column with an Agilent 7683 injector and flame ionisation detection. The fatty acid methyl esters were identified and quantified by gas chromatography. Results are given as relative percentages of the various fatty acids identified in specimens from the two fjords. Differences in average fatty acids proportions between sampled depths in Isfjorden and Rijpfjorden were analysed using one-way ANOVA. Differences in the fatty acid profile between fjords was analysed using the Mann–Whitney U test (sum rank test).
Mid-winter maturity status
Chaetognaths are hermaphrodites possessing male and female gonads (Alvarino 1992). The male gonads (testes and seminal vesicles) produce and secrete sperm, whilst the female gonads (ovaries and seminal receptacles) produce ova and receive sperm from a sexual partner (Alvarino 1992). Mature P. elegans are characterised by advanced ovaries containing large oocytes, high sperm loads filling the tail section of the animal, and pairs of pronounced seminal receptacles and vesicles (Russell 1932; Choe et al. 2003). We described the maturity of 29 of the largest individuals (40–51 mm, 8 × 1 mm size classes), and in 323 smaller individuals (13–39 mm, 26 × 1 mm size classes) in MIK samples from all fjords (both years, Table S1). Measured individuals were stained with borax carmine solution to highlight their gonads (Pierce 1941). Maturity assessments were made under the binocular microscope following the methods of Grigor et al. (2014). Ovaries were measured to the nearest 0.1 mm, and individuals showing advanced ovary development were taken as those with ovaries ≥5.4 mm, whilst shorter ovaries <5.4 mm were considered to be poorly developed. The volume of loose sperm in the tail was estimated to the nearest 25 %. Low sperm loads could suggest that sperm is filling the tail or has already been released. However, when sperm has already been released, remaining sperm appears more sparsely distributed. Therefore, spent individuals can be separated from those which have not yet secreted sperm. The size of the seminal receptacles was also described (see Grigor et al. 2014 for further details).
Results
Chaetognath abundance and prey field
In Rijpfjorden in 2012, the most abundant zooplankton taxa based on the MPS sampling were the larger calanoid copepod Calanus finmarchicus and the smaller copepods Pseudocalanus spp. (Calanoida) and Oithona similis (Cyclopoida) (Table 1). P. elegans was the most abundant chaetognath, but overall chaetognaths were also less abundant than the non-copepod taxa Oikopleura spp. (Tunicata) and Beroe cucumis (Ctenophora) (Table 1). Average P. elegans abundances in Rijpfjorden MIK trawls ranged from 2.4 ± 1.2 ind. m−3 (225 m) to 14.7 ± 7.0 ind. m−3 (75 m), comparing relatively well with the MPS results. For further details on the polar night zooplankton community in Rijpfjorden, see Webster et al. (2013), Kraft et al. (2013), and Daase et al. (2014).
Gut contents
Observed gut contents were copepods, chitinous debris, lipid droplets, and other detritus that could not be identified. The proportions of P. elegans with gut contents (FCRmax) varied between fjords and years (Kruskal–Wallis test, P < 0.01), ranging from 9 to 53 % (Fig. 2). Some individuals contained two or more gut content types. Lipid droplets were the most common gut observation overall (Figs. 3, 4), observed in 106 (11.7 %) of the 903 chaetognaths. A total of 33 individuals (3.7 %) contained identifiable copepod prey, including C. finmarchicus, Metridia longa, and harpacticoid copepods. Proportions containing prey (FCRmin) also varied between fjords and years (Kruskal–Wallis test, P < 0.05), ranging from 0 to 10 % (Fig. 2). FRs ranged from 0.00 to 0.24 prey ind.−1 day−1 (mean = 0.12 prey ind.−1 day−1). Amongst feeding P. elegans, median per haul proportions with prey varied with fjord (Kruskal–Wallis test, P < 0.005), but proportions with other gut contents did not (P > 0.05, Fig. 3). The relationship between chaetognath head width and body length was linear (y = 0.031x + 0.37, R 2 = 0.79), and prey was identified in individuals with head widths ≥0.87 mm (Fig. 4). Per haul proportions of P. elegans with gut contents increased with head width size class (Kruskal–Wallis test, P < 0.05, Fig. 4), but amongst feeders, proportions with each gut content type did not vary with head width (P > 0.05, Fig. 4).
Body composition
Values for carbon and nitrogen isotopes varied little between fjords and sampling depths (Table 2). The δ13C content of P. elegans ranged from −22.0 ± 0.3 ‰ (Rijpfjorden) to −21.5 ± 0.2 ‰ (Isfjorden), whilst δ15N content ranged from 12.5 ± 0.3 ‰ to 12.9 ± 0.1 ‰ (both Isfjorden). Average trophic level (TL) based on the δ15N content was 2.9, and average C/N ratio over fjords and depths was 3.1 (Table 2).
Parasagitta elegans fatty acid signature did not differ between Isfjorden and Rijpfjorden (Mann–Whitney U test, P > 0.05). Similarly, percentage composition of almost all fatty acids did not differ between sampling depths in the two fjords (one-way ANOVA, P > 0.05). The high levels of 18:1(n-9) compared with those of 18:1(n-7) strongly indicate carnivory. The Calanus fatty acid marker 20:1(n-9) and 22:1(n-11) were both abundant. The dinoflagellate marker docosahexaenoic acid (DHA) 22:6(n-3) was recorded in interestingly high amounts, whilst eicosapentaenoic acid (EPA) 20:5(n-3) was recorded in moderate to high amounts (Table 3).
Mid-winter maturity status
Amongst the largest individuals in the population (body lengths 40–51 mm), 55 % had well-developed seminal receptacles, whereas 93 % had relatively high sperm volumes in their tails (50–100 %) and all had advanced ovaries (≥5.4 mm). In smaller individuals (lengths 13–39 mm), 45 % had well-developed seminal receptacles and 42 % had advanced ovaries; 80 % had synthesised relatively high sperm volumes (filling 50–100 % of the tail area).
Discussion
Studies during the polar night
Studies of plankton ecology in the high Arctic are typically restricted to spring and summer, due to the logistical difficulties of sampling during winter. The polar night cruise, of which our study formed a part, offered unique possibilities for a better understanding of polar night marine ecology (see Berge et al. 2012; Webster et al. 2013; Daase et al. 2014).
We show that the common chaetognath P. elegans remains an active carnivore during the polar night. This arrow worm is not in a dormant and non-feeding state although its feeding rates may be considerably lower than in spring and summer, and reproduction is absent (or occurring at very low rates) at this time of year. These observations add to the increasing awareness of activity levels and ecological interactions of pelagic organisms during the polar night.
Feeding activity and rates
Copepods are abundant in Svalbard fjords during winter (Table 1) and as a non-visual predator P. elegans should, as opposed to fish and other visual predators, be able to encounter and catch them also during the polar night. The behaviour of some copepods during the polar night (i.e. diapausing in an unalert state) could make them particularly susceptible to predators such as chaetognaths (Darnis et al. 2012). About a quarter of the P. elegans individuals showed signs of recent feeding (mainly lipid droplets). The body of P. elegans does not contain a centralised oil vacuole, suggesting that all lipid droplets in P. elegans guts remained from recently digested prey, yet only 4 % contained identifiable prey. Based on Feigenbaum’s (1982) digestion time estimate of 10.2 h at 0 °C (likely longer in colder waters), our findings would suggest that most individuals had not fed for several hours before their capture. In contrast, Falkenhaug (1991) identified prey in 36 % of the P. elegans population collected from the Barents Sea in summer. Our average estimate of per capita feeding in P. elegans was 0.12 prey ind.−1 day−1, corresponding to 0.66 prey ind.−1 m−3 consumed per day by the P. elegans population (based on the abundance data in Table 1). Their predation impact on Calanus is therefore low given the high Calanus abundances (Table 1, see also Daase et al. 2014). Calanus are assumed to be in a dormant, non-feeding state during winter (Falk-Petersen et al. 2009). Daase et al. (2014) reported on Calanus from the same cruise in 2012 and found that the C. finmarchicus population in Rijpfjorden comprised mainly copepodite stage CV and some CIVs, which could indicate overwintering. However, these authors also noted that “the bulk of the C. finmarchicus and C. glacialis population was found close to the surface and not at greater depth where they presumably should overwinter”, suggesting they were not in an unalert dormancy phase, at least not in January. If not, these copepods may be more alert to the presence of predators than assumed, allowing them to avoid or escape chaetognaths.
Our FR estimate is similar to that obtained for immature specimens in northern Sweden in autumn–winter (0.2 prey ind.−1 day−1) and much lower than reported for spring–summer (0.7–0.9 prey ind.−1 day−1, Øresland 1987). These findings suggest that feeding rates for P. elegans at high-latitude drop during winter, corresponding well with the reduced growth rates observed during winter (Grigor et al. 2014), and the lack of reproduction at this time of year (this study). In less seasonal environments, energetic requirements may vary less between the seasons, accounting for relatively higher feeding rates at lower latitudes, also during winter and early spring (e.g. up to 1.33 prey ind.−1 day−1 in Vineyard Sound, Feigenbaum 1982).
However, care should be taken when comparing estimates of feeding rates between studies, because each study detects prey with various levels of precision. For example, we did not search for mandible remains in guts to detect further signs of recent copepod digestion, as in other studies (e.g. Falkenhaug 1991; Giesecke and Gonzalez 2004). Feeding estimates in chaetognaths are also highly sensitive to sampling methodology. As well as the risk of damage to their fragile bodies, stressed chaetognaths may also regurgitate gut contents, leading to underestimates of true feeding rates. Baier and Purcell (1997) estimated prey loss from guts of ~50 % when tows were longer than 2 min. Such short hauls can be unfeasible, especially when using large trawl nets such as the MIK, and when sampling populations in deeper waters. Further studies should therefore utilise new zooplankton imaging devices for observing chaetognaths in the water column without necessarily capturing them in nets. Optical methods are promising avenues (e.g. Schulz et al. 2010, Sainmont et al. 2014).
Energetics
Stable isotope and FATM analyses confirmed the position of P. elegans as a predator during the polar night. From its average trophic level (TL) of 2.9, it can be classified as a carnivore according to a trophic model devised for the European Arctic (Søreide et al. 2006, in which carnivores had TLs between 2.9 and 3.3). δ15N (an indicator of protein content) and δ13C (an indicator of organic matter content) values varied little with station or depth, and agree well with values from the Barents Sea in March (Søreide et al. 2006). δ15N values were, however, lower than reported from the Bering Sea (14.7 ± 0.7 ‰, Lovvorn et al. 2005), suggesting that P. elegans in the sub-Arctic Pacific typically feed higher up the food chain (also see “Lipid profile” section of “Discussion”). Many factors affect the elemental composition of zooplankton species, including age, size maturity status, reproductive strategies, as well as variations in the composition and growth rates of primary producers (Ikeda 1974), yet for P. elegans in the Barents Sea, δ15N values seem to vary little throughout the year. Søreide et al. (2006) reported δ15N values ranging from 11.9 ± 0.2 ‰ in spring to 12.2 ± 0.1 ‰ in winter. δ13C values varied slightly more between spring (−19.3 ± 0.8 ‰) and winter (−20.8 ± 0.3 ‰).
Lipid profile
The high levels of the FATMs 20:1(n-9) and 22:1(n-11) confirm that P. elegans is part of the Arctic Calanus-based food web (Falk-Petersen et al. 2007; Wold et al. 2011). Furthermore, the high level of 22:6(n-3) indicates that the base of the food chain is dominated by dinoflagellates, which can be very abundant in waters north of Svalbard in spring–summer (Hegseth and Sundfjord 2008). This lipid signature agrees well with previous results from the European sub-Arctic (Falk-Petersen et al. 1987). Both the presence of green detritus in P. elegans guts, and the surprisingly high levels of the dinoflagellate fatty acid marker docosahexaenoic acid, may suggest that some omnivory and/or detritivory occurs. Non-carnivorous feeding in chaetognaths has been suggested by Casanova et al. (2012). In the Canadian Arctic, Eukrohnia hamata and Pseudosagitta maxima have been observed ingesting green detritus under the microscope (pers. obs.). Eukrohnia species in Antarctica were found to contain higher amounts of 16:0, reflecting feeding on the Antarctic copepods including Rhincalanus gigas (Kruse et al. 2010). We suggest that winter feeding is captured in these results, following the suggestions of Graeve et al. (2005) that a new signal (source of food) will be visible relatively quickly (~4–8 days after feeding). If P. elegans starves during autumn and early winter, and if 22:6(n-3) undergoes little transformation or metabolism (Dalsgaard et al. 2003), it remains possible that this signal is retained from feeding on Calanus during a previous summer or autumn bloom (Falk-Petersen et al. 1990; Wold et al. 2011). However, this study and others (e.g. Feigenbaum 1982; Øresland 1987; Falkenhaug 1991) suggest a low likelihood of long-term fasting in P. elegans.
An important difference between the two main chaetognath species in Arctic waters, Eukrohnia hamata and P. elegans, is that the former typically possess oil vacuoles in the centre of their bodies, whilst the latter do not (Øresland 1990; Pond 2012, pers. obs.). Previous studies that considered the presence of oil vacuoles in this species to reflect recent feeding may therefore have overestimated feeding rates (e.g. Samemoto 1978; Froneman and Pakhomov 1998). The role of these vacuoles in E. hamata (e.g. buoyancy, storage) is not yet clear (Pond 2012), but unlike P. elegans, E. hamata has a cosmopolitan range. In the Arctic, both species commonly occur at epipelagic depths (Bieri 1959), but in the North Pacific, E. hamata populations are commonly found residing deeper, and throughout a wider depth range than P. elegans (e.g. Bieri 1959; Alvarino 1964). Maintaining such wide vertical distributions would certainly require a strong control of buoyancy, which could be offered by having centrally positioned oil vacuoles (Pond 2012). Lipid reserves form a central component of the life history of polar zooplankton species (Falk-Petersen et al. 2009; Varpe et al. 2009). In Antarctica, 34 % of examined Eukrohnia bathyantarctica individuals were found to contain oil droplets in summer compared to 57 % of individuals in winter. This species also contained relatively high amounts of the fatty acid 18:1(n-9), which is found in storage lipids (Kruse et al. 2010). If the primary role of oil vacuoles is storage, this could suggest that survival and possibly reproduction in E. hamata is less dependent on concurrent food intake than in P. elegans. Studies of the extent of capital breeding (Varpe et al. 2009) in E. hamata are therefore needed.
Reproduction
Small P. elegans <10 mm were absent from MPS samples from January, and whilst many individuals above 20 mm had synthesised high sperm loads, many of the largest P. elegans specimens in the nets (40–51 mm) still lacked well-developed seminal receptacles, suggesting that they were not fully mature (Russell 1932; Choe et al. 2003). Grigor et al. (2014) frequently observed pronounced receptacles in individuals ≥20 mm from February to May, after which they disappeared. In the Arctic, breeding of P. elegans generally takes place in spring and summer (Kramp 1939; Ussing 1939; Bogorov 1940; Grigor et al. 2014); this is in contrast to Eukrohnia hamata, which may also reproduce in winter (pers. obs.). Saito and Kiørboe (2001) showed that P. elegans <5 mm in the North Sea fed almost exclusively on prey <350 μm in length. In Svalbard, the prey in this size range is represented by small cyclopoids such as O. similis, as well as Calanus nauplii and the young Calanus stages. In winter, individuals may be waiting for increased food input before they reproduce or for suitable food for the young and newborns to feed on (in terms of size and availability). By investing energy into maturation during winter, egg hatching can be scheduled to coincide with the reproduction of a wide range of copepod prey in spring and summer, and the buoyancy of chaetognath eggs may also allow them to hatch in shallow waters (Hagen 1999 and references therein) amongst their grazing prey. In another fjord on Svalbard, large numbers of P. elegans eggs became visible in the water column in March 2003, suggesting that conditions for reproduction begin to improve at this time of year (Hirche and Kosobokova 2011).
Concluding remarks
Knowledge of an organism’s activity level and foraging ecology outside of the windows of the main primary production period (spring–summer) is key to establishing an understanding of the full annual routine (Varpe 2012). Here, we have focused on the polar night feeding ecology of a predatory zooplankter, adding to recent work on the life history and vertical distribution (Grigor et al. 2014). We found the chaetognath P. elegans to feed during the polar night, but with feeding rates lower in winter than have been reported from other seasons. As growth rates (e.g. Dunbar 1962; Grigor et al. 2014) may also decrease at this time and reproduction does not seem to occur, individuals should have lower-energy requirements and require less food. Although several copepod taxa may rest at depth in an unalert state during the Arctic winter, our study shows that mortality on copepods caused by P. elegans in the water column is low at this time of year. This study did, however, not sample the zone immediately above the seafloor, the hyperbenthic zone, where high densities of chaetognaths are known to aggregate during winter (Choe and Deibel 2000), and where abundances of resting copepods may also peak. The activities of P. elegans in this zone, as well as those of the other Arctic species Eukrohnia hamata and Pseudosagitta maxima, require more attention.
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Acknowledgments
We are grateful to the crew and scientific staff on the two R/V Helmer Hanssen cruises for their help in sampling and especially Carl Ballantine. We wish to thank Clare Webster and Dr. Katarzyna Blachowiak-Samolyk for providing the zooplankton community data from Rijpfjorden in 2012, and Ida Helene Funderud Kallevik and Eike Stübner for MPS data from Isfjorden ISA in 2012. Thanks also to the Institute for Energy Technology for running the stable isotope analyses, UNILAB for running the fatty acid analyses, and Anette Wold and Janne Søreide for suggestions and comments on the fatty acid and isotope results. JG and AM received stipends from Québec-Océan, and gratefully acknowledge Québec-Océan and Professor Louis Fortier for their scientific support. This is a contribution towards the two projects Marine Night (226417) and Circa (214271), funded by the Research Council of Norway.
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Grigor, J.J., Marais, A.E., Falk-Petersen, S. et al. Polar night ecology of a pelagic predator, the chaetognath Parasagitta elegans . Polar Biol 38, 87–98 (2015). https://doi.org/10.1007/s00300-014-1577-8
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DOI: https://doi.org/10.1007/s00300-014-1577-8