Abstract
Tobramycin is an essential and extensively used broad-spectrum aminoglycoside antibiotic obtained through alkaline hydrolysis of carbamoyltobramycin, one of the fermentation products of Streptoalloteichus tenebrarius. To simplify the composition of fermentation products from industrial strain, the main byproduct apramycin was blocked by gene disruption and constructed a mutant mainly producing carbamoyltobramycin. The generation of antibiotics is significantly affected by the secondary metabolism of actinomycetes which could be controlled by modifying the pathway-specific regulatory proteins within the cluster. Within the tobramycin biosynthesis cluster, a transcriptional regulatory factor TobR belonging to the Lrp/AsnC family was identified. Based on the sequence and structural characteristics, tobR might encode a pathway-specific transcriptional regulatory factor during biosynthesis. Knockout and overexpression strains of tobR were constructed to investigate its role in carbamoyltobramycin production. Results showed that knockout of TobR increased carbamoyltobramycin biosynthesis by 22.35%, whereas its overexpression decreased carbamoyltobramycin production by 10.23%. In vitro electrophoretic mobility shift assay (EMSA) experiments confirmed that TobR interacts with DNA at the adjacent tobO promoter position. Strains overexpressing tobO with ermEp* promoter exhibited 36.36% increase, and tobO with kasOp* promoter exhibited 22.84% increase in carbamoyltobramycin titer. When the overexpressing of tobO and the knockout of tobR were combined, the production of carbamoyltobramycin was further enhanced. In the shake-flask fermentation, the titer reached 3.76 g/L, which was 42.42% higher than that of starting strain. Understanding the role of Lrp/AsnC family transcription regulators would be useful for other antibiotic biosynthesis in other actinomycetes.
Key points
• The transcriptional regulator TobR belonging to the Lrp/AsnC family was identified.
• An oxygenase TobO was identified within the tobramycin biosynthesis cluster.
• TobO and TobR have significant effects on the synthesis of carbamoyltobramycin.
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Introduction
Streptoalloteichus tenebrarius, also known as Streptomyces tenebrarius (Tamura et al. 2008), is an actinomycete that produces carbamoyltobramycin through fermentation. The fermented products are commonly utilized in the industry for producing tobramycin via alkaline hydrolysis (Koch et al. 1973). Tobramycin is an essential and extensively used broad-spectrum antibiotic belonging to the aminoglycoside (Park et al. 2013). It was primarily employed clinically for treating severe infectious diseases caused by Gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa (Wang et al. 2012; Wasserman et al. 2015). Additionally, it exhibited bactericidal effect, even in treating multi-drug resistant microorganisms (MDR) (Pagkalis et al. 2011; Rosalia et al. 2022). The antibacterial mechanism of action involves the binding of tobramycin to the aminoacyl-tRNA recognition site (A-site) on the 30S subunit of bacterial ribosomes, which prevents formation of the normal 70S complex and inhibits protein translation ultimately leading to bacterial death. Furthermore, the deoxygenation at C-3′ reduces the susceptibility to phosphorylation of tobramycin and thereby enhances its efficacy as an antibiotic (Kim et al. 2016).
In early years, traditional mutagenesis breeding techniques such as ultraviolet radiation (UV) and nitrosoguanidine (NTG) were primarily used to obtain high-yielding strains (Qattan and Khattab 2019). However, these traditional breeding methods have limitations of low effectiveness and a demand of an extensive time and labor investment. During the past two decades, various antibiotic biosynthetic gene clusters have been identified (Kudo and Eguchi 2009). The tobramycin biosynthesis gene clusters AJ579650 and AJ810851 and the apramycin biosynthesis gene cluster AJ629123 have been released in National Center for Biotechnology Information (NCBI) database (Kharel et al. 2004; Kudo and Eguchi 2009; Wehmeier and Piepersberg 2009). Genetic modification of the related genes could be carried out based on the analysis of gene clusters and modern molecular biology technology to improve the metabolic and production performance of strains. Firstly, blocking byproduct biosynthesis pathway may increase the proportion of main product in fermentation (Hong and Yan 2012; Ni et al. 2011; Xiao et al. 2014). Secondly, increased copy number of entire biosynthetic gene clusters could also be beneficial to improve secondary metabolite production (Chen et al. 2010; Mitousis et al. 2021). However, since Streptomyces have the linear chromosomes and plasmids (Chen et al. 2002), such engineered high-yield strains may be genetically unstable due to the presence of large segments of duplication within the genomes. It was preferable to obtain the strains with comparatively stable fermentation in practical industrial production.
Streptoalloteichus tenebrarius Tb used in this study is an industrialized bacterium, mainly producing apramycin, carbamoyltobramycin, and a small amount of carbamoylkanamycin B. Based on the research regarding the aminoglycoside secondary metabolites with 2-deoxystreptamine structure, apramycin, tobramycin, and kanamycin B were all synthesized from the initial substrate D-glucose (Kudo et al. 2021). The key intermediate paromamine was obtained through a series of reactions from D-glucose. Due to the low substrate selectivity of dehydrogenase TobQ, aminotransferase TobB, glucosyltransferase TobM2, and aminoacyltransferase TobZ (Park et al. 2011), there are parallel pathways in the biosynthesis of tobramycin and kanamycin B (Fig. 1) (Ni et al. 2011; Parthier et al. 2012; Tamegai et al. 2002). 6′-Oxolividamine is the last common intermediate splitting the tobramycin and apramycin pathways and is produced from paromamine via sequential dehydration (AprD4), deoxygenation (AprD3), and dehydrogenation (AprQ) reactions (Kim et al. 2016; Kudo et al. 2017; Lv et al. 2016). Then, 7′-N-acetyl-demethylaprosamine with the unique bicyclic octose core structure is synthesized through the aldolase AprG (Fan et al. 2023; Oconnor et al. 1976). To minimize the cytotoxicity, AprU, AprP, and AprI may perform acetylation, phosphorylation, and methylation modifications on intermediates (Sun et al. 2022; Zhang et al. 2022a). Finally, apramycin was synthesized through glycosylation and elimination of phosphate group (Zhang et al. 2021).
With the understanding of synthesis pathways, it is possible to redirect metabolism towards the biosynthesis of tobramycin by knocking out key genes in the apramycin synthesis pathway. However, due to the different metabolic backgrounds of the starting strains, blocking the synthesis of byproduct apramycin may not always produce good results. For example, after knocking out the aprH-M gene by Hong (Hong and Yan 2012), the biosynthesis of apramycin was blocked in Streptomyces tenebrarius Tt49 and metabolism flowed towards the biosynthesis of carbamoyltobramycin, resulting in a significant increase in the fermentation titer of carbamoyltobramycin. Xiao et al. (2014) knocked out the NDP-octodiose synthase gene aprK, and the production of carbamoyltobramycin was increased by 9% in S. tenebrarius Tt49, which was different from the 35% of decrease in Streptoalloteichus tenebrarius 2444 reported by Mitousis (Mitousis et al. 2021).
The biosynthetic gene cluster of secondary metabolites in Streptomyces usually includes structural genes, regulatory genes, and resistance genes. Secondary metabolites synthesis is usually regulated by pathway-specific or global regulatory proteins. The generation of antibiotics can be significantly affected by altering relevant regulatory proteins, especially the pathway-specific regulatory proteins within the cluster (Liu et al. 2019, 2017a, 2021). The leucine-responsive regulatory protein (Lrp/AsnC) family is widespread in bacteria and archaea (Ziegler and Freddolino 2021), which regulates a variety of cellular activities (Brinkman et al. 2003; Peeters and Charlier 2010), such as amino acid metabolism, virulence, motility, nutrient transport, stress tolerance, and antibiotic resistance. The role of Lrp/AsnC family transcription regulators in regulating the biosynthesis of secondary metabolite in the actinomycetes system was still insufficient understanding. It has been reported that Lrp/AsnC family proteins negatively regulate the biosynthesis of erythromycin (Liu et al. 2019, 2017a, 2021) and bitespiramycin (Lu et al. 2019) and positively regulate actinorhodin (Liu et al. 2017b; Yu et al. 2016), thaxtomin A (Liu et al. 2023), and lincomycin (Xu et al. 2020, 2023), while there have been no reports on the metabolic synthesis of aminoglycoside so far.
In this study, a mutant mainly producing carbamoyltobramycin was constructed with apramycin blocked through gene disruption. Due to the presence of common intermediates in the biosynthesis process of apramycin and tobramycin, the regulation of carbon flux through the apramycin and tobramycin pathways appears to be entangled. Blocking the pathway of apramycin may affect the biosynthesis of tobramycin. Due to the differences in the background expression of the producing strains, blocking the reconstruction of metabolic pathways through byproduct biosynthesis may not necessarily lead to completely positive results. So we conducted research on the regulation of tobramycin biosynthesis. We investigated a transcriptional regulator TobR located in the tobramycin biosynthetic gene cluster. The structure of TobR has shown similarity to the Lrp/AsnC family. Gene tobR deletion and overexpression strains were constructed to investigate its regulatory role in carbamoyltobramycin production. In vitro electrophoretic mobility shift assay (EMSA) assays verified that TobR directly regulated its neighboring gene tobO by interacting with the promoter fragments of tobO. Additionally, we constructed two tobO overexpression strains with different promoters that both greatly improved the production of carbamoyltobramycin. Finally, the combination of tobR disruption and tobO overexpression resulted in an engineered strain with a higher yield of carbamoyltobramycin compared with respective tobR disruption or tobO overexpression strains.
Materials and methods
Bacterial strains and general fermentation and growth conditions
Strains in this study are listed in Table 1. The parental strain S. tenebrarius Tb (referred as Tb) was obtained from Livzon Pharmaceutical Group Inc. (Guangdong, China). E. coli DH5α (TransGen Biotech, China) was used as the host to construct, maintain, and amplify plasmids. E. coli BL21(DE3) was used for protein expression (Invitrogen). E. coli ET12567/pUZ8002 was a kind gift from Professor Yiling Du (Zhejiang University, China). E. coli ET12567/pUZ8002 was used for conjugation to transform plasmids into S. tenebrarius strains.
E. coli was cultured in LB liquid medium (1% w/v tryptone, 0.5% w/v yeast extract, and 1% w/v NaCl) or on LB agar plates at 37 °C, 220 rpm. All S. tenebrarius strains were grown on ISP4 solid medium (BD, USA) for spore preparation or conjugation and in yeast extract-malt extract (YEME, 0.3% w/v yeast extract, 0.3% w/v malt extract, 2.5% w/v sucrose, 0.5% w/v polypeptone and 1% w/v glucose) liquid medium for preparation of genomic DNA and seed medium. The fermentation medium (5% w/v soyabean powder, 1% w/v fish meal, 1% w/v corn flour, 0.8% w/v NH4Cl, 0.6% w/v silkworm powder, 0.7% w/v CaCO3, 3% w/v soya-bean oil, 0.025% w/v CaCl2, and 1.5% w/v glucose) were used for production of carbamoyltobramycin.
For culturing E. coli ET12567/pUZ8002 carrying related constructed plasmid used for intergeneric conjugation, antibiotics were supplemented to growth media at the following final concentrations: kanamycin, 25 μg/mL; spectinomycin, 50 μg/mL; and chloramphenicol, 25 μg/mL. For intergeneric conjugation, after co-culturing for about 20 h, spectinomycin and nalidixic acid were coated on ISP4 agar plates at the final concentration of 100 μg/mL and 25 μg/mL, respectively.
Plasmid construction
All plasmids and primers used in this study are listed in Tables S1 and S2, respectively. The full-length nucleotide sequence of streptomycin 3″-adenylyltransferase (Protein ID: QID24729.1) gene spc was codon-optimized for S. tenebrarius and ordered from Sangon Biotech (Shanghai, China) as a synthetic DNA, which replaced the gene aac(3)IV of vectors pOJ260 (Changsha Yingrun Biotechnology Co., Ltd, Hunan, China) and pIJ8660 (Sun et al. 1999) and constructed the plasmids pSpc260 and pIJ8660-spc. Then, nucleotide sequence of promoters ermEp* (Bibb et al. 1994) and kasOp* (Wang et al. 2013) were inserted into the vector pIJ8660-spc with the XhoI and BglII sites, respectively, and constructed the plasmids pSpc8660 and pSpc-kasOp*. Plasmids pSpc260, pSpc8660, and pSpc-kasOp* derived from pOJ260 and pIJ8660 were used for genome editing in S. tenebrarius.
The gene cassette tobR was amplified from the genomic DNA of Tb using primer pair 28-tobR-F/R. Plasmid vector fragment was amplified from pET28a( +) using primer pair 28a-V-F/R. The cassette fragments were then individually cloned into pET28a( +) using the ClonExpress II one-step cloning kit (Vazyme biotech, Nanjing, China) and confirmed by sequencing, yielding plasmids pET28a-tobR for gene expression in E. coli BL21(DE3).
Accordingly, the overexpression plasmids pSpc8660-tobR and pSpc8660-tobO were also constructed as mentioned above, using primer pairs v152-F/R, ermE-tobR-F/R, and ermE-tobO-F/R. The overexpression plasmid pSpc-kasOp*-tobO was constructed by primers V-kasOp-tobO-F/v152-R and tobO-F/ermE-tobO-R.
For deletion of the intergenic region within the gene aprJ, two DNA fragments flanking the region were amplified from the genomic DNA of Tb using primer pairs aprJ-F1/R1 and aprJ-F2/R2, respectively. Plasmid vector fragment was amplified from pSpc260 using primer pairs v1139-F/R and connected the homologous arms at both ends through fusion PCR using primer pair aprJ-F1/R2 and then cloned into pSpc260 generating the disruption plasmid pSpc260-△aprJ. Besides, the disruption plasmid pSpc260-△aprK, pSpc260-△aprQ, pSpc260-△aprI, pSpc260-△aprM, pSpc260-△tobR, and pSpc260-△tobO constructed as mentioned above, using primer pairs aprK-F1/R1/F2/R2, aprQ-F1/R1/F2/R2, aprI-F1/R1/F2/R2, aprM-F1/R1/F2/R2, tobR-F1/R1/F2/R2, and tobO-F1/R1/F2/R2.
Construction of S. tenebrarius strains
For gene deletion, suicide plasmids pSpc260-△aprJ, pSpc260-△aprK, pSpc260-△aprQ, pSpc260-△aprI, pSpc260-△aprM, pSpc260-△tobR, and pSpc260-△tobO derived from pOJ260 were transformed into E. coli ET12567/pUZ8002 and then introduced into S. tenebrarius by conjugation. Single crossover recombination strains were selected by culturing the transformants on ISP4 plates which containing 100 μg/mL spectinomycin at 37 °C. Subsequently, after three rounds of sporulation on plates without antibiotics, double crossover mutants were selected (Figs. S1, S2, and S3). To verify the genotype of double exchange strains, primer pairs di-aprJ-F/R, di-aprK-F/R, di-aprQ-F/R, di-aprI-F/R, di-aprM-F/R, di-tobR-F/R, and di-tobO-F/R were used for PCR. Name the successfully constructed strains as Tb-△aprJ, Tb-△aprK, Tb-△aprQ, Tb-△aprI, Tb-△aprM, Tb-△aprJ-△tobR, and Tb-△aprJ-△tobO.
The overexpression plasmids pSpc8660-tobR, pSpc8660-tobO, and pSpc-kasOp*-tobO were introduced into S. tenebrarius as mentioned above. To get the overexpression strains, exconjugants were selected on ISP4 agar plates supplemented with spectinomycin and identified by PCR. These overexpression strains were named as Tb-△aprJ/ermEp*-tobR, Tb-△aprJ/ermEp*-tobO, Tb-△aprJ/kasOp*-tobR, and Tb-△aprJ-△tobR /ermEp*-tobO, respectively. The empty vector pSpc8660 was also transferred into Tb-△aprJ and Tb-△aprJ-△tobR to generate control strains named Tb-△aprJ/pSpc8660 and Tb-△aprJ-△tobR/pSpc8660.
Heterologous expression and purification of TobR
For heterologous expression of TobR protein in E. coli BL21(DE3), the tobR was amplified by PCR from the genome of Tb with the primer pair 28-tobR-F/R. Then, it was cloned into pET28a and generating an expression plasmid with N-terminal His-tag fusion. The constructed plasmid pET28a-tobR was introduced into E. coli BL21(DE3), and the protein expression was induced with IPTG at a final concentration of 0.1 mM at 30 °C for 10 h. His6-tagged TobR protein was extracted and purified on a Ni2+-NTA spin column (Shenggong). The quality of the purified protein was estimated by sodium dodecyl sufate polyacrylamide gel electrophoresis (SDS-PAGE). The protein concentration was measured by Pierce BCA Protein Assay Kit (Shanghai, Thermo Fisher Scientific Co., Ltd).
Electrophoretic mobility shift assays (EMSAs)
The EMSAs were performed as described previously (Hellman and Fried 2007). PCR was performed using Tb-△aprJ genome as a template and ptobO-F/R primers to obtain the fragment named as PtobO probe, which is the intergenic sequence between tobR and tobO. Moreover, the promoter regions of each transcriptional unit in the tobramycin biosynthesis cluster were amplified by PCR with 7 pairs of primers, including ptobE-F/R, ptobT-F/R, ptobB-F/R, ptobZ-F/R, ptobS1-F/R, ptobM1-F/R, and ptobA-F/R, respectively (Table S2). These probes were named PtobE, PtobT, PtobB, PtobZ, PtobS1, PtobM1, and PtobA. The 100 ng DNA probes were incubated individually with various concentrations of His6-tagged TobR in binding buffer (10 mL pH 7.5 Tris–HCl, 5 mM MgCl2, 60 mM KCl, 10 mM DTT, 50 mM EDTA, and 10% glycerol) at 30 °C for 20 min in 20 μL reaction mixture. After incubation, the samples were fractionated on 6% native PAGE gels in ice-cold 0.5 × TBE buffer at 100 V for 120 min.
Fermentation
The strains were cultured on MS agar plates for about 5–7 days at 37 °C for sporulation. The spores were then inoculated into 30 mL YEME medium (seed medium) in 250-mL flasks and cultured at 37 °C, 220 rpm for 20 h. The seed culture was then inoculated into the 30 mL fermentation medium giving a 3% vaccination dose and then cultured at 37 °C, 220 rpm for 144 h.
Quantitative analysis
Dilute the supernatant to a certain ratio and derivatize it with 2% 2,4-dinitrofluorobenzene and then filter it through a Millipore membrane. The aminoglycosides antibiotics were analyzed as previously described (Barends et al. 1987) with some modification. For the analysis of tobramycin, culture samples from fermentation were centrifuged at 10,000 g for 5 min to remove the mycelia; the supernatants were derivatized with 2% 2,4-dinitrofluorobenzene and filtered through a Millipore membrane (pore diameter, 0.22 μm). Samples were analyzed by high-performance liquid chromatography (HPLC) through the C-18 column (Hypersil BDS 5 μm, 4.6 mm × 250 mm), with a UV detector at 365 nm. A 0.01 mM ammonium acetate aqueous solution (the pH adjusted with phosphoric acid to 4.0)/acetonitrile (47: 53, v/v) was used as the mobile phase with an elution rate of 1 mL/min. All results were reported as the average of biological triplicates.
RNA preparation and qRT-PCR assay
Cells of Tb-△aprJ, Tb-△aprJ/ermEp*-tobO, and Tb-△aprJ/kasOp*-tobO grown in fermentation medium for 24 h, 72 h, and 120 h were harvested by centrifugation. Total RNA was collected using the EASYspin Plus RNA extraction/purification kit (Aidlab Biotechnologies Co., Ltd). The integrality and quantity of the RNA were detected by 1% agarose gel electrophoresis and a microplate reader. RNA samples were treated by reverse transcription using the ReverTra AceTM qPCR RT Master Mix with gDNA Remover kit (TOYOBO). The obtained cDNAs were used as templates for qPCR. qPCR was employed on the TransStart Top Green qPCRSuperMix (TransGen Biotech) using corresponding primers listed in Table S2. A 20 μL reaction mixture contained 10 μL 2 × TransStart Top Green qPCRSuperMix, 0.4 μL cDNA, 0.4 μL per primer (about 0.2 μM), and 8.8 μL RNase-free ddH2O. The running conditions were 95 °C for 10 min (step 1), 30 cycles of 94 °C for 10 s, 56 °C for 10 s, and 72 °C for 10 s (step 2). Each experiment was carried out with three independent biological replicates and three experimental replicates. As its constant transcriptional level between Tb-△aprJ and its derivatives, the endogenous gapA gene was used as an internal control to normalize samples.
Bioinformatics analysis
Online tools are available for protein homology analysis, multiple sequence alignment and structure prediction. The BLAST search engine provided by the National Center for Biotechnology Information (https://blast.ncbi.nlm.nih.gov/Blast.cgi) was used for protein homology analysis. Online comparison tool CLUSTALW online sequence alignment tool (https://www.genome.jp/tools-bin/clustalw/) was used for multiple sequence alignment. The ΑlphaFold 2 (https://colab.research.google.com/github/sokrypton /ColabFold/blob/main/ΑlphaFold2.ipynb) was used to predict the structure of TobR and TobO.
Results
Elimination of byproduct apramycin
To investigate the effect of blocking the biosynthesis of byproduct apramycin on the production of carbamoyltobramycin, we knocked out several key enzymes in the biosynthesis pathway of apramycin including putative phosphosugar mutase AprJ, NDP-octose synthase AprK, aminoglycoside 6′-dehydrogenase AprQ, N-methyltransferase AprI, and glycosyltransferase AprM. As shown in Fig. 2a, in comparison with the parent strain Tb, Tb-△aprJ, Tb-△aprK, Tb-△aprQ, and Tb-△aprI showed comparable biosynthesis of carbamoyltobramycin, while the production of apramycin was significantly decreased to less than 1% of the production of carbamoyltobramycin. However, the biosynthesis of tobramycin in Tb-△aprM was severely impacted with the production of carbamoyltobramycin decreased to 0.34 g/L, only 12.8% of the initial strain (2.64 g/L), and the byproduct apramycin was still synthesized although its yield (0.09 g/L) was decreased to 23% of the original level (0.39 g/L) (Fig. 2b). Gene aprM putatively encoding glycosyltransferase might be functionally similar to TobM1, a putative aminoglycoside 4-glucosaminyltransferase, or TobM2, a putative 6-glucosyltransferase, which participated in tobramycin and apramycin pathway (Fig. S4 and table S3). In addition, the Tb-△aprQ and Tb-△aprI mutants showed new peaks on the HPLC spectrum. Due to the blockage of the biosynthetic pathway of apramycin, there may be an accumulation of intermediate products, and the new peak appeared after the aprQ was knocked out could be lividamine or 6′-dehydro-6′-oxoparomamine (Wang et al. 2021). Furthermore, the new compound appearing in the Tb-△aprI strain might be N’-demethyl-apramycin (Zhang et al. 2022a). As for Tb-△aprJ and Tb-△aprK, the fermentation of carbamoyltobramycin was not affected, and the byproduct was almost completely removed. And there was no significant changes observed in the process of cell growth and spore synthesis for Tb-△aprJ and Tb-△aprK. The mutant Tb-△aprJ was selected for further research due to its slightly higher production of 2.64 g/L carbamoyltobramycin compared with 2.59 g/L of Tb-△aprK. Overall, blocking the biosynthesis of apramycin in this work did not have a significant impact on the production of carbamoyltobramycin. Actually, the effect of blocking apramycin synthesis on production of carbamoyltobramycin was strain-specific. For example, by blocking the biosynthesis of apramycin in strains S. tenebrarius Tt49, the production of carbamoyltobramycin was increased by sixfold in the study of Hong (Hong and Yan 2012) and by 9% in the study of Xiao (Xiao et al. 2014). However, Lena Mitousis et al. found that blocking the biosynthesis of apramycin decreased the production of carbamoyltobramycin by 35% in S. tenebrarius 2444 (Mitousis et al. 2021). We found that the starting strains S. tenebrarius Tt49 that Xiao and Hong used mainly produced apramycin, while the proportion of apramycin and carbamoyltobramycin in S. tenebrarius 2444 that Lena Mitousis used was similar. And the proportion of apramycin was only 14.8% of carbamoyltobramycin in our starting strain S. tenebrarius Tb. Hence, effect of knocking out the gene of apramycin biosynthesis pathway on the carbamoyltobramycin synthesis would be strain-specific.
Bioinformatics analysis of TobR in S. tenebrarius
With the strain eliminating the production of main byproduct, we attempted to improve the yield of carbamoyltobramycin in the engineered strains by transcriptional regulation reconstruction. After analyzing the whole tobramycin biosynthesis gene cluster (Table S3), we found a gene tobR that may encode a transcriptional regulator TobR, and no other genes in the cluster may encode transcriptional regulators. According to the prediction results of BlastP, TobR may belong to the the Lrp/AsnC family. We first carried out a multiple sequence alignment between TobR and other Lrp/AsnC family members. TobR exhibited about 20% sequence identity with most of previously reported Lrp homologs in PDB database, with the highest similarity with the Lrp/AsnC of E. coli, reaching 29.37% (Fig. S5). Furthermore, TobR showed high sequence similarity of 68.89–88.27% with its homologues from actinomycetes such as Streptoalloteichus hindustanus (WP_073480789.1), Actinokineospora alba (WP_228769743.1), Alloactinosynnema sp. L-07 (CRK55752.1), Nonomuraea sp. KC401 (WP_138203008.1), and Kribbella antibiotica (WP_138203008.1) (Fig. S6). Therefore, the understanding of TobR function in this study might be useful for illustrating the role of Lrp/AsnC family transcription regulators in other actinomycetes.
Although the Lrps homologues from different sources exhibited low sequence conservation characteristics (Kawashima et al. 2008), their structure was highly similar (Peeters and Charlier 2010). As shown in Fig. 3, it typically consists of two domains including one N-terminal DNA binding structure with a common helix-turn-helix folding (HTH motif) and the other with a typical αβ-ligand binding domain of sandwich folding at C-terminal (Ettema et al. 2002). These two domains are linked by a flexible loop with a length of approximately 15 amino acids (de los Rios and Perona 2007; Reddy et al. 2008). In the regulation process of biological reaction, Lrps proteins normally function with status of multimer including dimer, tetramer, hexamer, octamer, and dodecamer that are usually observed for other Lrp/AsnC family transcription regulators (Brinkman et al. 2000; Koike et al. 2004; Leonard et al. 2001; Pritchett et al. 2009). The transition of different association conformations may influence the interaction effect between DNA and protein (Jeong et al. 2015). The structural model of TobR was predicted using Alphafold 2, and the plDDT score indicates the high prediction accuracy (Fig. S7). Then, the structure of TobR was compared with the structures of other Lrp/AsnC family transcriptional regulators reported in the PDB database (Fig. 3). TobR exhibits the Lrp/AsnC family structural feature that HTH motif is linked to the αβ-ligand binding domain through a flexible loop, suggesting that TobR belongs to Lrp/AsnC family.
Inactivation and overexpression of tobR in S. tenebrarius
The mutant strain Tb-△aprJ-△tobR and the starting strain Tb-△aprJ were carried out to investigate the impact of TobR on S. tenebrarius growth. The cells were collected and weighted during the fermentation process, and the growth and sporulation of each strain were observed during the cultivation on MS solid medium at 37 °C. There was no significant difference in wet bacterial mass and mycelial growth or spore formation between the two strains, indicating that TobR may not play a role in regulating mycelial growth and morphological differentiation (Fig. 4a&4b).
The strains Tb-△aprJ, Tb-△aprJ-△tobR, Tb-△aprJ/ermEp*-tobR, and their control strain Tb-△aprJ/pSpc8660 were also fermented in shake flasks to investigate the production yield. As shown in the Fig. 4c, in comparison with the parent strain Tb-△aprJ, Tb-△aprJ-△tobR improved the production of carbamoyltobramycin by 22.35%, from 2.64 to 3.23 g/L. However, overexpression of the tobR in Tb-△aprJ reduced the production of target product by 10.32%, from 2.56 to 2.37 g/L. These results suggested that the Lrp/AsnC family transcription regulator TobR might have a negative effect on the carbamoyltobramycin biosynthesis in S. tenebrarius.
qRT-PCR and EMSA analysis of TobR
The yield of carbamoyltobramycin was increased in TobR knockout strains, suggesting that TobR negatively regulates the biosynthesis of tobramycin. qRT-PCR assay was performed in further investigate the impact of TobR at 72 h in fermentation. As shown in Fig. 5, the transcription levels of the adjacent gene tobO and each transcription unit related to the tobramycin biosynthetic pathway were compared in Tb-△aprJ and Tb-△aprJ-△tobR strains. The transcription levels of the transcription units tobO, tobB, tobE, and tobZ in the Tb-△aprJ-△tobR significantly decreased compared with the control group Tb-△aprJ, while the changes in tobS1 and tobM1 were not significant. Moreover, the genes involved in these transcription units were all speculated to be genes in the tobramycin biosynthesis pathway. Furthermore, the transcription level of tobT, which is presumed to encode the transporter (Fig. 7f), increased by 9.3-fold compared to the original level.
Due to the inability to summarize patterns from changes in transcription levels of relevant genes, we further confirmed the mechanism of TobR through EMSA analysis. We expressed His6-tagged TobR in E. coli BL21(DE3) and examined its affinity to PtobO (tobR-tobO-int) with EMSA. With the induction of IPTG, BL21-TobR effectively expressed the target protein with a size of 21.5 kDa, consistent with the predicted size of TobR-His6 fusion protein (Fig. 6a). The fusion protein was well-expressed in soluble form and mainly distributed in the supernatant without formation of inclusion bodies. Purification through a Ni2+-NTA spin column from the supernatant yielded pure TobR protein (Fig. 6a). A TobR-PtobO complex formed in a concentration-dependent manner was observed, and the higher the concentration of TobR added, the more complex formed (Fig. 6b).
Besides the interaction between TobR and its neighboring genes, we created 500 bp DNA probes containing promoter regions for various transcription units within the gene cluster to investigate whether TobR directly regulates synthetic genes by analyzing their DNA–protein binding status in EMSA experiments (Fig. 6c). Under the 2 μM concentrations of TobR, there was no obvious binding between TobR and each probe (Fig. 6d). Further, improve the protein concentrations of TobR to 5 μM, but no binding was found (Fig. S8), indicating that there was no direct regulation relationship between TobR and genes within the tobramycin biosynthesis gene cluster.
Bioinformatics analysis of TobO
According to the qRT-PCR and EMSA results of TobR, the Lrp/AsnC family regulator TobR might affect the synthesis of tobramycin by directly acting on its adjacent gene tobO (Fig. 6). We then investigated the involvement of TobO in the biosynthesis of carbamoyltobramycin in S. tenebrarius using bioinformatics tools. The tobO is a 984 bp gene encoding a protein TobO consisting of 327 amino acids. A sequence alignment was performed with the sequence of TobO as input using the tool of protein blast in the NCBI database. The results showed that TobO possessed a sequence identity of 71.33% and 70.32% with TauD/TfdA family dioxygenase from Actinokineospora diospyrosa (WP_253887134.1) and Actinokineospora sp. PR83 (WP_236229521.1), respectively. Additionally, it showed a sequence identity of 60.32% with L-asparagine oxygenase from Streptomyces rubradiris (GHH24494.1) suggesting that tobO might encode an α-ketoglutarate-dependent non-haem iron enzyme. Sequence and structure alignment were then performed between TobO and the known structure asparagine oxygenase (PDB ID: 2OG5) from Streptomyces coelicolor (Strieker et al. 2007), showing a sequence similarity of 47.0% (Fig. 7a). It was determined that His142, Glu144, and His278 were the catalytic triad of TobO, which formed active center with ferrous ion (Fig. 7b). Generally, L-asparagine oxygenase is responsible for the oxidation of L-asparagine and converting α-ketoglutarate into succinate and CO2 (Fig. 7c). Based on the sequence analysis and structural characteristics of TobR and L-asparagine oxygenase, TobO might be an amino acid oxidase, responsible for the related metabolic reactions of amino acids in the organism.
Inactivation and overexpression of tobO in S. tenebrarius
To explore the effect of tobO on carbamoyltobramycin production, the shake flask fermentation yield of starting strain Tb-△aprJ, overexpression strains Tb-△aprJ/ermEp*-tobO, Tb-△aprJ/kasOp*-tobO, a knockout strain Tb-△aprJ-△tobO, and an empty vector control Tb-△aprJ/pSpc8660 were measured. The titer of carbamoyltobramycin was increased by 36.36% and 22.84% in the overexpression strains ermEp*-tobO (3.60 g/L) and kasOp*-tobO (3.24 g/L) compared with the starting strain Tb-△aprJ (2.64 g/L), while there was no significant change in Tb-△aprJ-△tobO (2.71 g/L) (Fig. 8a). The biomass during fermentation were also analyzed, and the wet cell cumulant of Tb-△aprJ/ermEp*-tobO (0.148 g/mL) was slightly higher than that of starting strain Tb-△aprJ (0.135 g/mL) (Fig. 8b), indicating that the overexpression of tobO might promote the growth of strains during the fermentation process. However, there was no significant difference observed in the sporulation status in MS solid medium between Tb-△aprJ/ermEp*-tobO and Tb-△aprJ-△tobO (Fig. 8c).
In order to further determine the role of tobO, we measured the transcripts of tobO at mycelial growth (24 h), product accumulation (72 h), and stabilization stage (120 h) during the fermentation process in Tb-△aprJ, Tb-△aprJ/ermEp*-tobO, and Tb-△aprJ/kasOp*-tobO by qRT-PCR. The transcriptional level of tobO in Tb-△aprJ after 72 h and 120 h of growth was increased by 6.2- and 2.8-folds, respectively, compared with 24 h (Fig. 8d), indicating that tobO may play a role in the synthesis of secondary metabolites. In addition, the transcripts of tobO in overexpressed strains Tb-△aprJ/ermEp*-tobO and Tb-△aprJ/kasOp*-tobO at 72 h was increased by 2.4- and 1.7-folds respectively compared with in Tb-△aprJ, suggesting that the overexpression of tobO did improve the transcripts of tobO. However, ermEp* showed higher promoter activity compared with kasOp* in this experiment, different from previous literature (Wang et al. 2013). The different bacterial species may be the key reason. The strain used in this experiment was Streptoalloteichus tenebrarius, belonging to Pseudoocardiaceae, while the Streptomyces coelicolor, Streptomyces venezuelae, and Streptomyces avermitilis in which the kasOp* was tested belong to Streptomycetaceae (Bai et al. 2015; Dong et al. 2020; Myronovskyi and Luzhetskyy 2016).
Moreover, both knocking out tobR and overexpressing tobO increased the fermentation titer of carbamoyltobramycin, and TobR could directly interact with DNA fragments of the PtobO intergenic region. tobO was a possible target gene regulated by TobR, which negatively regulates biosynthesis of tobramycin by inhibiting transcriptional expression of tobO. However, the knockout of the tobO did not have a significant impact on the production of carbamoyltobramycin (Fig. 7a). The transcripts of tobO during fermentation of Tb-△aprJ and Tb-△aprJ-△tobR strains indicate that the transcription level of tobO was rapidly decreased by 0.02-, 0.29- and 0.08-folds compared with Tb-△aprJ at 24 h, 72 h, and 120 h, respectively, after blocking tobR, which demonstrate that TobR has a positive regulatory effect on the expression of the tobO.
In addition, the transcription status of each transcription unit in the tobramycin biosynthesis gene cluster of Tb-△aprJ/ermEp*-tobO was also measured. As shown in Fig. 7e, the transcription levels of all transcription units were significantly increased in the overexpressing strains. Among them, tobT showed the highest increase in transcription levels, which was 32.8-fold of the original level.
Combination of tobR disruption and tobO overexpression
Both tobR knockout and tobO overexpression promoted the fermentation production of carbamoyltobramycin. We hence wondered if the additive effect could be achieved by combining tobR knockout and tobO overexpression. Production of carbamoyltobramycin was detected in shake flask fermentation after overexpressing the tobO following the knockout of the tobR. The mutant strain Tb-△aprJ-△tobR/ermEp*-tobO produced carbamoyltobramycin with a higher titer compared with strains with single modifications. The shaking flask fermentation level of this strain reached 3.76 g/L (Fig. 9), a significant increase of 42.42% compared with the Tb-△aprJ strain. The results suggested that the combination of tobR knockout and tobO overexpression showed a beneficially additive impact on the carbamoyltobramycin biosynthesis.
Discussion
In this study, the mutant strain Tb-△aprJ mainly producing carbamoyltobramycin was first selected with the main byproduct apramycin completely blocked by gene disruption of aprJ. The elimination of fermentation by-product from industrial production strain can significantly improve product quality while reducing the input of production costs during the purification process.
Intriguingly, a novel Lrp-like protein TobR was identified from tobramycin biosynthesis cluster of S. tenebrarius with bioinformatics analysis. The gene lrp is typically found in close proximity to its target gene (Peeters and Charlier 2010). For example, in Sulfolobus solfataricus (Song et al. 2013), LysM directly regulates the adjacent lysWXJK operon. In Halobacterium salinarum R1 (Schwaiger et al. 2010), LrpA1 inhibits its neighboring aspB3. SACE_Lrp in Saccharopolyspora erythraea suppresses the transcription level of its adjacent gene SACE_4838 and affects erythromycin biosynthesis (Liu et al. 2017a). Additionally, the Lrp/AsnC family transcription regulator SCO3361 found in Streptomyces coelicolor directly regulates the expression of its neighboring gene SCO3362 (Liu et al. 2017b). The similarity among the Lrp/AsnC family implies that TobR may directly bind with tobR-tobO-int and control the expression of tobO. As expected, through the EMSA analysis of TobR, we found it directly combined with the promoter region of the tobO operon rather than other transcription units in tobramycin biosynthesis cluster. Meanwhile, disruption of tobR and overexpression of tobO increased carbamoyltobramycin production by 22.34% (3.23 g/L) and 36.36% (3.60 g/L), respectively, compared with the parent strain Tb-△aprJ.
Furthermore, this work reveals that the transcription of tobO is positively regulated by TobR, but TobR may negatively regulate the biosynthesis of tobramycin through other regulation, and the regulatory mechanism of TobR still remains questionable. The TobR in this study may differ from the Lrp/AsnC family transcription regulatory factors reported in the literature that directly inhibit the expression of adjacent genes (Liu et al. 2019). The increase in tobO transcription level can significantly promote the biosynthesis of tobramycin, while the knockout of tobO did not have an inhibitory effect. Therefore, the increase of tobramycin synthesis caused by the knockout of tobR was likely due to the presence of other regulated key genes throughout the entire genome in S. tenebrarius. TobR may have other binding sites outside of the tobramycin biosynthesis gene cluster range. For example, SSP_Lrp was a global regulator directly affecting the expression of three positive regulatory genes, and it was a negative regulator involved in the spiramycin and bitespiramycin biosynthesis (Lu et al. 2019). In the biosynthesis regulation of actinomycins, it was found that SCO3361 simultaneously affects the transcription levels of whiB, ssgB, and amfC although SCO3361 only bound with amfC (Liu et al. 2017b).
Moreover, by measuring transcription levels of tobT, TobR inhibits the transcription of tobT through indirect regulation. The transporter TobT may play a very important role in the biosynthesis of tobramycin, and TobR may be involved in the efflux of products or substrate uptake during the secondary metabolism of tobramycin. An increase in TobT transcription level can promote the biosynthesis of tobramycin. And protein TobO was identified as an α-ketoglutarate-dependent non-haem iron amino acid oxidase in S. tenebrarius with bioinformatics analysis. Members of the α-ketoglutarate-dependent non-haem iron enzyme superfamily are widely found in prokaryotes, eukaryotes, and archaea. These enzymes typically require Fe2+ as a metal cofactor and α-ketoglutarate as a co-substrate for catalysis of various reactions such as hydroxylation, ring cleavage, C–C bond cleavage, cis–trans isomerization, desaturation, intramolecular peroxidation, and heterocycle formation (Gao et al. 2018). Overexpression of tobO promoted the biosynthesis of carbamoyltobramycin, and no synthesis blockade or inhibition phenomenon was observed in the knockout strain. Protein function analysis suggested that the catalytic reaction of TobO was not involved in the biosynthetic pathway of carbamoyltobramycin. TobO does not directly provide precursor substances for biosynthesis of carbamoyltobramycin or modify secondary metabolites and intermediate products. The α-ketoglutarate were produced through transamination reactions. Subsequently, under the catalytic action of α-ketoglutarate-dependent non-haem iron enzymes, succinate is generated from α-ketoglutarate and enters central metabolism (Hu et al. 2022; Zhang et al. 2022b). The overexpression of TobR may enhance the decomposition and utilization of amino acids, thereby providing more energy and material for central metabolism through the tricarboxylic acid cycle. Under conditions of higher material and energy utilization efficiency, TobO might promote the growth of mycelium at the primary metabolic stage and provide more producer for the production of secondary metabolite when entering the product synthesis stage. Additionally, adjusting the metabolic flux leads to a reduction in glucose metabolism while increasing the use of amino acids in the system. More glucose is directed towards secondary metabolic synthesis pathways such as carbamoyltobramycin biosynthesis, which requires sufficient precursor materials. As a result, the overexpression of TobO promotes the bacterial growth and the biosynthesis of secondary metabolite.
Finally, the combination strain with tobR knockout and tobO overexpression has a beneficially additive impact on the production of carbamoyltobramycin, increased by 42.42% (3.76 g/L) compared with strain Tb-△aprJ (2.64 g/L). The engineered strains obtained in this study are potentially useful in producing carbamoyltobramycin in the industrial field. In addition, the exploration of novel transcriptional regulatory factors TobR and oxidase TobO in this article can also provide new research ideas for the efficient production of other secondary metabolites.
Data availability
The authors declare that all the data supporting the findings of this study are available within the paper, and its Supplementary Information is available from the corresponding author on request.
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Acknowledgements
We would like to thank Livzon Pharmaceutical Group Inc. (Guangdong, China) for their kind help in providing the Streptoalloteichus tenebrarius Tb.
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This work was financially supported by National Key Research and Development Program (2019YFA0905404).
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FY: methodology, investigation, writing-original draft. JYQ, CXT, and ZL: methodology, investigation. XHL, WMB, and YLR: conceptualization, supervision. YHR and LJP: conceptualization, revision, supervision. All authors read and approved the manuscript.
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Feng, Y., Jiang, Y., Chen, X. et al. Improving the production of carbamoyltobramycin by an industrial Streptoalloteichus tenebrarius through metabolic engineering. Appl Microbiol Biotechnol 108, 304 (2024). https://doi.org/10.1007/s00253-024-13141-2
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DOI: https://doi.org/10.1007/s00253-024-13141-2